ES2807530T3 - Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona - Google Patents
Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Download PDFInfo
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- ES2807530T3 ES2807530T3 ES17151869T ES17151869T ES2807530T3 ES 2807530 T3 ES2807530 T3 ES 2807530T3 ES 17151869 T ES17151869 T ES 17151869T ES 17151869 T ES17151869 T ES 17151869T ES 2807530 T3 ES2807530 T3 ES 2807530T3
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- Investigating Or Analysing Biological Materials (AREA)
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| JP5753720B2 (ja) * | 2010-04-22 | 2015-07-22 | アークレイ株式会社 | バイオセンサ |
| CN103003440B (zh) | 2010-07-23 | 2015-11-25 | 霍夫曼-拉罗奇有限公司 | 含两性离子缓冲液的组合物及其在电分析装置和方法中的用途 |
| EP2465936A1 (en) | 2010-12-20 | 2012-06-20 | LEK Pharmaceuticals d.d. | Enzymatic synthesis of statins and intermediates thereof |
| AU2011355209B2 (en) | 2010-12-20 | 2015-09-17 | Lek Pharmaceuticals D.D. | Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof |
| WO2012084194A1 (en) | 2010-12-22 | 2012-06-28 | Roche Diagnostics Gmbh | Systems and methods to compensate for sources of error during electrochemical testing |
| CN102559624B (zh) * | 2012-03-05 | 2013-10-16 | 浙江德清汇宁生物科技有限公司 | 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法 |
| EP2839267B1 (de) | 2012-04-19 | 2019-12-04 | F.Hoffmann-La Roche Ag | Verfahren und vorrichtung zur bestimmung einer analytkonzentration in blut |
| EP2811299A1 (en) | 2013-06-07 | 2014-12-10 | Roche Diagniostics GmbH | Test element for detecting at least one analyte in a body fluid |
| JP6895953B2 (ja) | 2015-09-25 | 2021-06-30 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ソルターゼａを利用してチオエステルを作製するための方法 |
| CN108026560A (zh) | 2015-09-25 | 2018-05-11 | 豪夫迈·罗氏有限公司 | 在低共熔溶剂中使用分选酶的转酰胺反应 |
| CN105331590B (zh) * | 2015-10-29 | 2020-02-28 | 英科隆生物技术（杭州）有限公司 | 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用 |
| CN105331591B (zh) * | 2015-10-29 | 2020-02-28 | 英科隆生物技术（杭州）有限公司 | PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置 |
| KR20180087329A (ko) | 2015-12-21 | 2018-08-01 | 에프. 호프만-라 로슈 아게 | 알칼리게네스 파에칼리스로부터의 돌연변이체 3-히드록시부티레이트 탈수소효소 뿐만 아니라 이의 관련 방법 및 용도 |
| JP6989528B2 (ja) | 2016-02-09 | 2022-01-05 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | ロドバクター・スフェロイデス（Ｒｈｏｄｏｂａｃｔｅｒ ｓｐｈａｅｒｏｉｄｅｓ）由来の変異型３−ヒドロキシブチレート脱水素酵素及びそれを含む方法 |
| CA3035874C (en) | 2016-10-05 | 2025-09-09 | F. Hoffmann-La Roche Ag | DETECTION REAGENTS AND ELECTRODE ARRANGEMENTS FOR MULTI-ANALYTICAL DIAGNOSTIC TEST ELEMENTS, AS WELL AS THEIR METHODS OF USE |
| CN115820608B (zh) * | 2022-08-31 | 2024-06-04 | 中国海洋大学 | λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用 |
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| DE3716957A1 (de) | 1987-05-20 | 1988-12-01 | Boehringer Mannheim Gmbh | Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten |
| DE4311464A1 (de) | 1993-04-08 | 1994-10-13 | Boehringer Mannheim Gmbh | Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase |
| DE19639169A1 (de) | 1996-09-24 | 1998-04-02 | Boehringer Mannheim Gmbh | Redoxaktive Verbindungen und deren Anwendung |
| JPH10243786A (ja) | 1997-03-03 | 1998-09-14 | Koji Hayade | 改変型グルコース脱水素酵素 |
| US5997817A (en) | 1997-12-05 | 1999-12-07 | Roche Diagnostics Corporation | Electrochemical biosensor test strip |
| JPH11243949A (ja) | 1998-03-03 | 1999-09-14 | Toyobo Co Ltd | Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法 |
| HUP0200285A2 (hu) * | 1999-02-19 | 2002-05-29 | Merck Patent Gmbh. | Glükóz-dehidrogenáz fúziós fehérjék és alkalmazásuk expressziós rendszerekben |
| JP2000350588A (ja) * | 1999-04-08 | 2000-12-19 | Koji Hayade | グルコース脱水素酵素 |
| TWI224136B (en) | 1999-04-30 | 2004-11-21 | Koji Sode | Glucose dehydrogenase |
| KR100519902B1 (ko) | 2000-10-27 | 2005-10-10 | 에프. 호프만-라 로슈 아게 | 가용성 피롤로퀴놀린 퀴논 의존성 글루코오스 디히드로게나아제의 변이체 |
| JP2004344145A (ja) * | 2002-05-27 | 2004-12-09 | Toyobo Co Ltd | 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体 |
| JP2004173538A (ja) * | 2002-11-25 | 2004-06-24 | Amano Enzyme Inc | ピロロキノリンキノン依存性グルコース脱水素酵素 |
| AU2005263591B2 (en) | 2004-07-20 | 2008-08-14 | F. Hoffmann-La Roche Ag | Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion |
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2007
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2008
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| JP5683809B2 (ja) | 2015-03-11 |
| BRPI0710131B1 (pt) | 2020-03-10 |
| HUE032804T2 (en) | 2017-11-28 |
| AU2007237478A1 (en) | 2007-10-25 |
| WO2007118647A1 (en) | 2007-10-25 |
| CN101421396B (zh) | 2016-01-20 |
| JP6185617B2 (ja) | 2017-08-23 |
| JP2013198497A (ja) | 2013-10-03 |
| EP2010649A1 (en) | 2009-01-07 |
| CA2649135C (en) | 2013-11-19 |
| EP3246402A1 (en) | 2017-11-22 |
| CA2649135A1 (en) | 2007-10-25 |
| JP2009533033A (ja) | 2009-09-17 |
| HUE049911T2 (hu) | 2020-11-30 |
| EP2010649B1 (en) | 2017-03-08 |
| KR20080106349A (ko) | 2008-12-04 |
| PL3246402T3 (pl) | 2020-10-19 |
| PT2010649T (pt) | 2017-05-03 |
| CN101421396A (zh) | 2009-04-29 |
| PT3246402T (pt) | 2020-07-14 |
| AU2007237478B2 (en) | 2011-05-19 |
| HK1131633A1 (zh) | 2010-01-29 |
| JP5937547B2 (ja) | 2016-06-22 |
| MX2008013001A (es) | 2008-10-17 |
| PL2010649T3 (pl) | 2017-08-31 |
| BRPI0710131A2 (pt) | 2011-08-02 |
| EP3246402B1 (en) | 2020-05-20 |
| ES2624805T3 (es) | 2017-07-17 |
| JP2016119904A (ja) | 2016-07-07 |
| KR101168623B1 (ko) | 2012-08-03 |
| US20090148874A1 (en) | 2009-06-11 |
| US7732179B2 (en) | 2010-06-08 |
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