JP2004506431A5 - - Google Patents

Download PDF

Info

Publication number
JP2004506431A5
JP2004506431A5 JP2002519680A JP2002519680A JP2004506431A5 JP 2004506431 A5 JP2004506431 A5 JP 2004506431A5 JP 2002519680 A JP2002519680 A JP 2002519680A JP 2002519680 A JP2002519680 A JP 2002519680A JP 2004506431 A5 JP2004506431 A5 JP 2004506431A5
Authority
JP
Japan
Prior art keywords
probe
nucleic acid
target nucleic
fluorescent
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2002519680A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004506431A (ja
JP5213297B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2001/025231 external-priority patent/WO2002014555A2/en
Publication of JP2004506431A publication Critical patent/JP2004506431A/ja
Publication of JP2004506431A5 publication Critical patent/JP2004506431A5/ja
Application granted granted Critical
Publication of JP5213297B2 publication Critical patent/JP5213297B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

JP2002519680A 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ Expired - Lifetime JP5213297B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US60/224,726 2000-08-11
US24061000P 2000-10-16 2000-10-16
US60/240,610 2000-10-16
PCT/US2001/025231 WO2002014555A2 (en) 2000-08-11 2001-08-10 Single-labeled oligonucleotide probes

Publications (3)

Publication Number Publication Date
JP2004506431A JP2004506431A (ja) 2004-03-04
JP2004506431A5 true JP2004506431A5 (enExample) 2008-10-09
JP5213297B2 JP5213297B2 (ja) 2013-06-19

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002519680A Expired - Lifetime JP5213297B2 (ja) 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ

Country Status (8)

Country Link
US (1) US6635427B2 (enExample)
EP (1) EP1307592B1 (enExample)
JP (1) JP5213297B2 (enExample)
AT (1) ATE332398T1 (enExample)
CA (1) CA2417986C (enExample)
DE (1) DE60121342T2 (enExample)
ES (1) ES2267803T3 (enExample)
WO (1) WO2002014555A2 (enExample)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
NZ521593A (en) * 2000-03-29 2004-11-26 Lgc Ltd Hybridisation beacon and method of rapid sequence detection and discrimination
CA2484215C (en) 2002-04-23 2015-02-24 Kirk Max Ririe Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
JP4377375B2 (ja) 2002-12-06 2009-12-02 エフ.ホフマン−ラ ロシュ アーゲー 病原性生物の多重分析検出
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
CA2525482C (en) 2003-04-04 2015-02-17 F. Hoffmann-La Roche Ag Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes
JP4454249B2 (ja) * 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
WO2005051967A2 (en) * 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
KR100885177B1 (ko) * 2004-04-12 2009-02-23 학교법인 포항공과대학교 표적 dna 또는 rna 탐지용 올리고뉴클레오티드
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
WO2006074222A2 (en) * 2005-01-03 2006-07-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
ES2285578T3 (es) 2005-01-18 2007-11-16 F. Hoffmann-La Roche Ag Visualizacion de señales de fluorescencia utilizando telecentricidad.
ES2284087T3 (es) 2005-01-18 2007-11-01 F. Hoffmann-La Roche Ag Generacion de imagenes de señales de fluorescencia mediante opticas telecentricas de excitacion y de generacion de imagenes.
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
EP1801114B8 (de) 2005-11-23 2009-12-02 Roche Diagnostics GmbH Polynukleotid mit Phosphatmimetikum
JP4713514B2 (ja) * 2006-02-20 2011-06-29 エフ.ホフマン−ラ ロシュ アーゲー 改善された標識試薬
CN103172700B (zh) 2006-02-27 2014-12-17 霍夫曼-拉罗奇有限公司 经由镁螯合的pcr热启动
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
EP3118333B1 (en) 2006-12-13 2019-04-03 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8247171B2 (en) * 2007-02-01 2012-08-21 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
KR101147277B1 (ko) * 2007-07-13 2012-05-21 아크레이 가부시키가이샤 Jak2 유전자의 변이 검출용 프로브 및 그 용도
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) * 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010135917A1 (zh) 2009-05-26 2010-12-02 厦门大学 一种检测核酸序列变异的方法
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
WO2011052754A1 (ja) * 2009-10-30 2011-05-05 アークレイ株式会社 Egfr遺伝子多型検出用プローブおよびその用途
JPWO2011062258A1 (ja) * 2009-11-19 2013-04-11 アークレイ株式会社 Mthfr遺伝子増幅用プライマーセット、それを含むmthfr遺伝子増幅用試薬およびその用途
JP5917144B2 (ja) * 2009-12-07 2016-05-11 アークレイ株式会社 疾患関連遺伝子の多型検出用プローブおよびその用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9285319B2 (en) 2010-04-21 2016-03-15 Riken Nucleic acid base analogs with quenching and fluorescent activities and applications thereof
JP5940771B2 (ja) 2010-07-12 2016-06-29 アークレイ株式会社 abl遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US20120276533A1 (en) * 2011-04-28 2012-11-01 Arkray, Inc. Method for Simultaneously Detecting Polymorphisms of Acetaldehyde Dehydrogenase 2 and Alcohol Dehydrogenase 2
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
EP2722388B1 (en) * 2011-06-16 2017-08-09 Nippon Steel & Sumikin Eco-Tech Corporation Nucleic acid probe for assaying nucleic acids
PT2535424E (pt) 2011-06-16 2015-10-29 Gendiag Exe Sl Snps associados a doença tromboembólica
WO2013034160A1 (en) * 2011-09-06 2013-03-14 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Methods for analyzing biological macromolecular complexes and use thereof
JP2013074888A (ja) * 2011-09-15 2013-04-25 Arkray Inc IL28B(rs8099917)とITPA(rs1127354)の変異を検出する方法
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
US20140213471A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
EP3567123B1 (en) 2013-05-20 2021-11-10 BioVentures, LLC Gep5 model for multiple myeloma
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
EP3189159A4 (en) 2014-06-26 2018-05-30 University of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
WO2016172632A2 (en) 2015-04-24 2016-10-27 Atila Biosystems, Inc Amplification with primers of limited nucleotide composition
CN113832215B (zh) * 2016-02-09 2024-02-06 荣研化学株式会社 对目标核酸进行检测的方法
MX2019001186A (es) 2016-07-29 2019-08-29 Juno Therapeutics Inc Metodos para evaluar la presencia o la ausencia de un virus capaz de replicacion.
JP2020526194A (ja) 2017-06-29 2020-08-31 ジュノー セラピューティクス インコーポレイテッド 免疫療法薬と関連する毒性を評価するためのマウスモデル
MX2020000900A (es) 2017-07-29 2021-01-08 Juno Therapeutics Inc Reactivos para expandir celulas que expresan receptores recombinantes.
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
CN111615556A (zh) 2017-11-01 2020-09-01 朱诺治疗学股份有限公司 产生t细胞组合物的方法
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
BR112021008738A2 (pt) 2018-11-06 2021-11-03 Juno Therapeutics Inc Processo para produzir células t geneticamente construídas
EP3955953A1 (en) 2019-04-17 2022-02-23 Alpine Immune Sciences, Inc. Methods and uses of variant icos ligand (icosl) fusion proteins
MX2021015317A (es) 2019-06-12 2022-03-11 Juno Therapeutics Inc Terapia de combinacion de una terapia citotoxica mediada por celulas y un inhibidor de una proteina de la familia bcl2 de prosupervivencia.
KR20220066892A (ko) 2019-08-22 2022-05-24 주노 쎄러퓨티크스 인코퍼레이티드 T 세포 요법 및 제스트 동족체 2의 인핸서 (ezh2) 억제제의 병용 요법 및 관련 방법
US20220340981A1 (en) * 2019-09-23 2022-10-27 Universiteit Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
MX2022008625A (es) 2020-01-13 2022-09-09 Aspen Neuroscience Inc Metodo de diferenciacion de celulas neuronales y composiciones relacionadas y metodos de uso.
US20230181641A1 (en) 2020-05-13 2023-06-15 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
KR20230042283A (ko) 2020-06-26 2023-03-28 주노 테라퓨틱스 게엠베하 재조합 수용체를 조건부로 발현하는 조작된 t 세포, 관련된 폴리뉴클레오티드 및 방법
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
US20250034662A1 (en) 2021-12-03 2025-01-30 Medicinal Genomics Corporation Psilocybe assay
CA3250467A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. METHODS FOR CLASSIFYING THE STATE OF DIFFERENTIATION OF CELLS AND ASSOCIATED COMPOSITIONS OF DIFFERENTIATED CELLS
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
EP0826066B1 (en) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
CA2253969A1 (en) 1996-05-15 1997-11-20 Rajkumar Siva Iyer Non-nucleotide linking reagents
CA2591550C (en) 1996-06-04 2009-04-28 University Of Utah Research Foundation Monitoring hybridization during pcr
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US6451530B1 (en) 1996-12-13 2002-09-17 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
US6465175B2 (en) * 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
CA2644688C (en) 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
SE9902565D0 (sv) 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
NZ521593A (en) 2000-03-29 2004-11-26 Lgc Ltd Hybridisation beacon and method of rapid sequence detection and discrimination

Similar Documents

Publication Publication Date Title
JP2004506431A5 (enExample)
JP5385973B2 (ja) 反応における複数の核酸配列の同時検出
US6833257B2 (en) Fluorimetric detection system of a nucleic acid
CN101831496B (zh) 多重定量核酸扩增及解链测定法
DK2828399T3 (en) SYSTEM FOR DETECTING POLYMERASE CHAIN REACTION USING OLIGONUCLEOTIDS INCLUDING A PHOSPHOROTHIOATE GROUP
US20120107820A1 (en) Multiplexed Quantitative PCR End Point Analysis of Nucleic Acid Targets
CN107435067B (zh) 用于在核酸测定中改善熔链分辨和多重性的探针
KR20120017033A (ko) 반응 카트리지에서의 다중 핵산 서열의 검출
KR20090100380A (ko) 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
EP3011056B1 (en) Real-time multiplexed hydrolysis probe assay
JP2008527979A (ja) 核酸の選択的増幅のための組成物、方法およびキット
JP2007515956A (ja) 改良された選択的ライゲーションおよび増幅アッセイ
KR20040024556A (ko) 검출 시스템
JP2006501836A (ja) 検出システム
JP2017521056A (ja) 鎖侵入に基づくdna増幅法
JP2006333739A (ja) 単分子計測による核酸分析方法
US20050130213A1 (en) Selective ligation and amplification assay
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
JP2003506068A5 (enExample)
CN100478453C (zh) 添加焦磷酸酶的实时pcr
EP1474529A2 (en) Specific multiplex analysis of nucleic acids
BE1026457B1 (fr) Procédé et dispositif pour la détection et différenciation de mutations ponctuelles chez des microorganismes par oligochromatographie
US9260746B2 (en) Photoinduced electron transfer (PET) primer for nucleic acid amplification
US20040175704A1 (en) Compositions and methods for polynucleotide sequence detection
Wabuyele et al. Detection of low abundant mutations in DNA using single-molecule FRET and ligase detection reactions