JP5213297B2 - 単一ラベルオリゴヌクレオチド・プローブ - Google Patents

単一ラベルオリゴヌクレオチド・プローブ Download PDF

Info

Publication number
JP5213297B2
JP5213297B2 JP2002519680A JP2002519680A JP5213297B2 JP 5213297 B2 JP5213297 B2 JP 5213297B2 JP 2002519680 A JP2002519680 A JP 2002519680A JP 2002519680 A JP2002519680 A JP 2002519680A JP 5213297 B2 JP5213297 B2 JP 5213297B2
Authority
JP
Japan
Prior art keywords
probe
nucleic acid
target nucleic
oligonucleotide
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2002519680A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004506431A5 (enExample
JP2004506431A (ja
Inventor
ウィットワー,カール,ティー.
クロケット,アンドリュー,オー.
カプリン,ブライアン,イー.
スティーヴンソン,ウェイド
ワグナー,ロリ,エー.
チェン,ジャン
クスカワ,ノリコ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UNIVERSITY OF UTHA RESEARCHFOUNDATION
Original Assignee
UNIVERSITY OF UTHA RESEARCHFOUNDATION
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNIVERSITY OF UTHA RESEARCHFOUNDATION filed Critical UNIVERSITY OF UTHA RESEARCHFOUNDATION
Publication of JP2004506431A publication Critical patent/JP2004506431A/ja
Publication of JP2004506431A5 publication Critical patent/JP2004506431A5/ja
Application granted granted Critical
Publication of JP5213297B2 publication Critical patent/JP5213297B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
JP2002519680A 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ Expired - Lifetime JP5213297B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22472600P 2000-08-11 2000-08-11
US60/224,726 2000-08-11
US24061000P 2000-10-16 2000-10-16
US60/240,610 2000-10-16
PCT/US2001/025231 WO2002014555A2 (en) 2000-08-11 2001-08-10 Single-labeled oligonucleotide probes

Publications (3)

Publication Number Publication Date
JP2004506431A JP2004506431A (ja) 2004-03-04
JP2004506431A5 JP2004506431A5 (enExample) 2008-10-09
JP5213297B2 true JP5213297B2 (ja) 2013-06-19

Family

ID=26918967

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002519680A Expired - Lifetime JP5213297B2 (ja) 2000-08-11 2001-08-10 単一ラベルオリゴヌクレオチド・プローブ

Country Status (8)

Country Link
US (1) US6635427B2 (enExample)
EP (1) EP1307592B1 (enExample)
JP (1) JP5213297B2 (enExample)
AT (1) ATE332398T1 (enExample)
CA (1) CA2417986C (enExample)
DE (1) DE60121342T2 (enExample)
ES (1) ES2267803T3 (enExample)
WO (1) WO2002014555A2 (enExample)

Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
US7998673B2 (en) 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
EP1715063B1 (en) * 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
AU2003281275A1 (en) 2002-04-23 2004-01-23 Idaho Technology, Inc. Sample withdrawal and dispensing device
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
US7122384B2 (en) * 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
EP1570079B1 (en) 2002-12-06 2010-07-21 Roche Diagnostics GmbH Multiplex assay detection of pathogenic organisms
US8137616B2 (en) 2003-04-04 2012-03-20 Roche Molecular Systems, Inc. System for multi color real time PCR
JP4454249B2 (ja) * 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
WO2005051967A2 (en) * 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
FR2866349B1 (fr) * 2004-02-12 2008-05-16 Biomerieux Sa Procede de diagnostic/pronostic d'une thrombose
US20080032413A1 (en) * 2004-04-12 2008-02-07 Byeang-Hyean Kim Oligonucleotide For Detecting Target Dna Or Rna
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
AU2006204087B2 (en) * 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
DE602005000749T2 (de) 2005-01-18 2007-12-06 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels telezentrischer Anregungs- und Abbildungsoptiken
DE602005000877T2 (de) 2005-01-18 2007-12-20 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels Telezentrizität
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
DE502005008153D1 (de) 2005-11-23 2009-10-29 Roche Diagnostics Gmbh Polynukleotid mit Phosphatmimetikum
US7759470B2 (en) * 2006-02-20 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
ES2364983T3 (es) 2006-02-27 2011-09-20 F. Hoffmann-La Roche Ag Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio.
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
WO2008020090A1 (es) * 2006-08-18 2008-02-21 Laboratorios Indas, S.A. Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa
CN102851369B (zh) * 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
EP2126114B1 (en) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Method for detection of presence of target polynucleotide in samples
US20100068713A1 (en) * 2007-07-13 2010-03-18 Arkray, Inc. Probe for detecting mutation in jak2 gene and use thereof
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
AU2009262870B2 (en) 2008-05-30 2014-03-20 Gen-Probe Incorporated Compositions, kits and related methods for the detection and/or monitoring of Salmonella
US9393566B2 (en) * 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010135917A1 (zh) 2009-05-26 2010-12-02 厦门大学 一种检测核酸序列变异的方法
WO2010147848A2 (en) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
JP5635496B2 (ja) * 2009-10-30 2014-12-03 アークレイ株式会社 Egfr遺伝子多型検出用プローブおよびその用途
KR101446556B1 (ko) * 2009-11-19 2014-10-01 아크레이 가부시키가이샤 Mthfr 유전자 증폭용 프라이머 세트, 그것을 포함하는 mthfr 유전자 증폭용 시약 및 그 용도
WO2011071046A1 (ja) * 2009-12-07 2011-06-16 アークレイ株式会社 疾患関連遺伝子の多型検出用プローブおよびその用途
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
WO2011132801A1 (ja) * 2010-04-21 2011-10-27 独立行政法人理化学研究所 消光性ならびに蛍光性の核酸塩基類似体とその応用
JP5940771B2 (ja) * 2010-07-12 2016-06-29 アークレイ株式会社 abl遺伝子多型の検出用プローブおよびその用途
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US20120276533A1 (en) * 2011-04-28 2012-11-01 Arkray, Inc. Method for Simultaneously Detecting Polymorphisms of Acetaldehyde Dehydrogenase 2 and Alcohol Dehydrogenase 2
US10557170B2 (en) 2011-06-16 2020-02-11 Gendiag.Exe, S.L. Thromboembolic disease markers
PT2535424E (pt) 2011-06-16 2015-10-29 Gendiag Exe Sl Snps associados a doença tromboembólica
WO2012173274A1 (ja) * 2011-06-16 2012-12-20 日鉄環境エンジニアリング株式会社 核酸測定用の核酸プローブ
JP5986207B2 (ja) * 2011-09-06 2016-09-06 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ 生体高分子複合体を分析するための方法およびその使用
JP2013074888A (ja) * 2011-09-15 2013-04-25 Arkray Inc IL28B(rs8099917)とITPA(rs1127354)の変異を検出する方法
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
EP4570924A3 (en) * 2013-01-24 2025-09-03 California Institute of Technology Chromophore-based characterization and detection methods
EP2999796B1 (en) 2013-05-20 2017-10-25 BioVentures, LLC Gep5 model for multiple myeloma
GB201319180D0 (en) * 2013-10-30 2013-12-11 Mast Group Ltd Nucleic acid probe
US10640833B2 (en) 2014-06-26 2020-05-05 University Of Florida Research Foundation, Inc. Rapid detection of infectious agents
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
US11091799B2 (en) 2015-04-24 2021-08-17 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
WO2017138484A1 (ja) * 2016-02-09 2017-08-17 栄研化学株式会社 標的核酸を検出する方法およびそれに用いる核酸プローブ
WO2018023094A1 (en) 2016-07-29 2018-02-01 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus
CA3067602A1 (en) 2017-06-29 2019-01-03 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
US20230242654A1 (en) 2017-07-29 2023-08-03 Juno Therapeutics, Inc. Reagents for expanding cells expressing recombinant receptors
US20210071258A1 (en) 2017-09-01 2021-03-11 Juno Therapeutics, Inc. Gene expression and assessment of risk of developing toxicity following cell therapy
CN107727618B (zh) * 2017-09-04 2019-12-31 江苏大学 生物芯片和特异性切割位点dna序列检测汞离子的方法
EP3704229B1 (en) 2017-11-01 2023-12-20 Juno Therapeutics, Inc. Process for producing a t cell composition
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3877054B1 (en) 2018-11-06 2023-11-01 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
WO2020214867A1 (en) 2019-04-17 2020-10-22 Alpine Immune Sciences, Inc. Methods and uses of variant icos ligand (icosl) fusion proteins
KR20220034782A (ko) 2019-06-12 2022-03-18 주노 쎄러퓨티크스 인코퍼레이티드 세포 매개 세포 독성 요법 및 친생존 bcl2 패밀리 단백질 억제제의 병용 요법
US20220298222A1 (en) 2019-08-22 2022-09-22 Juno Therapeutics, Inc. Combination therapy of a t cell therapy and an enhancer of zeste homolog 2 (ezh2) inhibitor and related methods
EP4034674B1 (en) * 2019-09-23 2025-03-26 Universiteit Gent Probe and method for str-genotyping
KR102107589B1 (ko) * 2019-11-20 2020-05-07 주식회사 유진셀 단일 신호 형광 물질을 이용한 실시간 타겟 핵산 검출 및 정량 방법
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
EP4090734B1 (en) 2020-01-13 2024-11-06 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
KR20230022868A (ko) 2020-05-13 2023-02-16 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 공여자-배치 세포의 제조 방법
US20240216508A1 (en) 2020-06-26 2024-07-04 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
KR20250007548A (ko) 2022-04-15 2025-01-14 아스펜 뉴로사이언스 인코포레이티드 세포의 분화 상태를 분류하는 방법 및 관련 분화된 세포 조성물
WO2023230581A1 (en) 2022-05-25 2023-11-30 Celgene Corporation Methods of manufacturing t cell therapies
AU2023343062A1 (en) 2022-09-13 2025-04-03 Medicinal Genomics Corporation Psilocybe assay
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin-Elmer Corp., Foster City Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
GB9602025D0 (en) * 1996-02-01 1996-04-03 Amersham Int Plc Nucleoside analogues
US5716784A (en) 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
EP0954606B1 (en) 1996-05-15 2003-10-15 Biogenex Laboratories Non-nucleotide linking reagents
PT912766E (pt) 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US6451530B1 (en) * 1996-12-13 2002-09-17 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
SE522077C2 (sv) * 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
DE19858588B4 (de) 1998-08-22 2016-04-07 Qiagen Gmbh Farbstoffmarkiertes Oligonukleotid zum Markieren eines Nukleinsäuremoleküls
US6699661B1 (en) * 1999-04-20 2004-03-02 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
SE9902565D0 (sv) 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination

Also Published As

Publication number Publication date
ATE332398T1 (de) 2006-07-15
ES2267803T3 (es) 2007-03-16
CA2417986A1 (en) 2002-02-21
US6635427B2 (en) 2003-10-21
CA2417986C (en) 2013-11-26
JP2004506431A (ja) 2004-03-04
EP1307592B1 (en) 2006-07-05
DE60121342T2 (de) 2007-06-28
DE60121342D1 (de) 2006-08-17
EP1307592A2 (en) 2003-05-07
WO2002014555A2 (en) 2002-02-21
WO2002014555A3 (en) 2003-02-27
US20030022177A1 (en) 2003-01-30

Similar Documents

Publication Publication Date Title
JP5213297B2 (ja) 単一ラベルオリゴヌクレオチド・プローブ
Gibson The use of real-time PCR methods in DNA sequence variation analysis
EP3543352B1 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay
JP3439221B2 (ja) 蛍光共鳴エネルギー移動を利用する核酸分析方法
US6287781B1 (en) Method for detection of target nucleic acids using PCR
US6642001B1 (en) Generic SBE-FRET protocol
Vet et al. Design and optimization of molecular beacon real-time polymerase chain reaction assays
JP2004033216A (ja) アンプリコン融解曲線分析による遺伝子型分析
CN101680029A (zh) 核酸检测
JP2011519570A (ja) 反応における複数の核酸配列の同時検出
US20140017689A1 (en) Method for detecting nucleic acids
US7504218B2 (en) Key probe compositions and methods for polynucleotide detection
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
Caplin et al. LightCycler hybridization probes
KR20170142128A (ko) Cto 세트를 사용한 pto 절단 및 연장 어세이에 의한 타겟 핵산서열의 검출
Melting Papers in Press. First published May 19, 2005 as doi: 10.1373/clinchem. 2005.051516
NZ706144B2 (en) Detection of target nucleic acid sequences by pto cleavage and extension assay

Legal Events

Date Code Title Description
RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20040924

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080811

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20080811

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110517

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110816

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110823

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110916

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110927

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20111017

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20111024

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111117

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120724

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121012

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20121019

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20121225

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130107

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130109

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130205

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130226

R150 Certificate of patent or registration of utility model

Ref document number: 5213297

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20160308

Year of fee payment: 3

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term