JP5213297B2 - 単一ラベルオリゴヌクレオチド・プローブ - Google Patents
単一ラベルオリゴヌクレオチド・プローブ Download PDFInfo
- Publication number
- JP5213297B2 JP5213297B2 JP2002519680A JP2002519680A JP5213297B2 JP 5213297 B2 JP5213297 B2 JP 5213297B2 JP 2002519680 A JP2002519680 A JP 2002519680A JP 2002519680 A JP2002519680 A JP 2002519680A JP 5213297 B2 JP5213297 B2 JP 5213297B2
- Authority
- JP
- Japan
- Prior art keywords
- probe
- nucleic acid
- target nucleic
- oligonucleotide
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22472600P | 2000-08-11 | 2000-08-11 | |
| US60/224,726 | 2000-08-11 | ||
| US24061000P | 2000-10-16 | 2000-10-16 | |
| US60/240,610 | 2000-10-16 | ||
| PCT/US2001/025231 WO2002014555A2 (en) | 2000-08-11 | 2001-08-10 | Single-labeled oligonucleotide probes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004506431A JP2004506431A (ja) | 2004-03-04 |
| JP2004506431A5 JP2004506431A5 (enExample) | 2008-10-09 |
| JP5213297B2 true JP5213297B2 (ja) | 2013-06-19 |
Family
ID=26918967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002519680A Expired - Lifetime JP5213297B2 (ja) | 2000-08-11 | 2001-08-10 | 単一ラベルオリゴヌクレオチド・プローブ |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6635427B2 (enExample) |
| EP (1) | EP1307592B1 (enExample) |
| JP (1) | JP5213297B2 (enExample) |
| AT (1) | ATE332398T1 (enExample) |
| CA (1) | CA2417986C (enExample) |
| DE (1) | DE60121342T2 (enExample) |
| ES (1) | ES2267803T3 (enExample) |
| WO (1) | WO2002014555A2 (enExample) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10027113A1 (de) * | 1999-12-23 | 2001-09-27 | Andreas Hoeft | Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens |
| FI20000333A0 (fi) * | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
| US7998673B2 (en) | 2000-03-29 | 2011-08-16 | Lgc Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
| EP1715063B1 (en) * | 2000-03-29 | 2011-01-26 | LGC Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
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| US7785776B2 (en) * | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
| JP2004024035A (ja) * | 2002-06-21 | 2004-01-29 | Tosoh Corp | 核酸の検出方法 |
| CA2433473A1 (en) * | 2002-07-23 | 2004-01-23 | F. Hoffmann-La Roche Ag | Fluorescent hybridization probes with reduced background |
| US7122384B2 (en) * | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
| US7718361B2 (en) | 2002-12-06 | 2010-05-18 | Roche Molecular Systems, Inc. | Quantitative test for bacterial pathogens |
| EP1570079B1 (en) | 2002-12-06 | 2010-07-21 | Roche Diagnostics GmbH | Multiplex assay detection of pathogenic organisms |
| US8137616B2 (en) | 2003-04-04 | 2012-03-20 | Roche Molecular Systems, Inc. | System for multi color real time PCR |
| JP4454249B2 (ja) * | 2003-04-18 | 2010-04-21 | アークレイ株式会社 | 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| WO2005051967A2 (en) * | 2003-11-19 | 2005-06-09 | Allelogic Biosciences Corp. | Oligonucleotides labeled with a plurality of fluorophores |
| FR2866349B1 (fr) * | 2004-02-12 | 2008-05-16 | Biomerieux Sa | Procede de diagnostic/pronostic d'une thrombose |
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| EP1634965B1 (en) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Real time PCR with the addition of pyrophosphatase |
| AU2006204087B2 (en) * | 2005-01-03 | 2010-09-16 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primer for nucleic acid detection |
| DE602005000749T2 (de) | 2005-01-18 | 2007-12-06 | Roche Diagnostics Gmbh | Fluoreszenzabbildung mittels telezentrischer Anregungs- und Abbildungsoptiken |
| DE602005000877T2 (de) | 2005-01-18 | 2007-12-20 | Roche Diagnostics Gmbh | Fluoreszenzabbildung mittels Telezentrizität |
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| US7759469B2 (en) | 2005-03-10 | 2010-07-20 | Roche Diagnostics Operations, Inc. | Labeling reagent |
| EP1700922B1 (en) | 2005-03-10 | 2016-08-24 | Roche Diagnostics GmbH | 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them |
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| DE502005008153D1 (de) | 2005-11-23 | 2009-10-29 | Roche Diagnostics Gmbh | Polynukleotid mit Phosphatmimetikum |
| US7759470B2 (en) * | 2006-02-20 | 2010-07-20 | Roche Diagnostics Operations, Inc. | Labeling reagent |
| ES2364983T3 (es) | 2006-02-27 | 2011-09-20 | F. Hoffmann-La Roche Ag | Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio. |
| RU2394915C2 (ru) * | 2006-03-24 | 2010-07-20 | Александр Борисович Четверин | Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления |
| WO2008020090A1 (es) * | 2006-08-18 | 2008-02-21 | Laboratorios Indas, S.A. | Método y kit para diagnosticar el riesgo de desarrollar una enfermedad trombótica venosa |
| CN102851369B (zh) * | 2006-12-13 | 2015-01-21 | 卢米耐克斯公司 | 用于实时pcr多重分析的系统和方法 |
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| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| AU2009262870B2 (en) | 2008-05-30 | 2014-03-20 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Salmonella |
| US9393566B2 (en) * | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
| EP2148187A1 (de) | 2008-07-25 | 2010-01-27 | Roche Diagnostics GmbH | Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion |
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| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ.ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| WO2010135917A1 (zh) | 2009-05-26 | 2010-12-02 | 厦门大学 | 一种检测核酸序列变异的方法 |
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| JP5635496B2 (ja) * | 2009-10-30 | 2014-12-03 | アークレイ株式会社 | Egfr遺伝子多型検出用プローブおよびその用途 |
| KR101446556B1 (ko) * | 2009-11-19 | 2014-10-01 | 아크레이 가부시키가이샤 | Mthfr 유전자 증폭용 프라이머 세트, 그것을 포함하는 mthfr 유전자 증폭용 시약 및 그 용도 |
| WO2011071046A1 (ja) * | 2009-12-07 | 2011-06-16 | アークレイ株式会社 | 疾患関連遺伝子の多型検出用プローブおよびその用途 |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| WO2011132801A1 (ja) * | 2010-04-21 | 2011-10-27 | 独立行政法人理化学研究所 | 消光性ならびに蛍光性の核酸塩基類似体とその応用 |
| JP5940771B2 (ja) * | 2010-07-12 | 2016-06-29 | アークレイ株式会社 | abl遺伝子多型の検出用プローブおよびその用途 |
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| JP2021511802A (ja) | 2018-01-31 | 2021-05-13 | ジュノー セラピューティクス インコーポレイテッド | 複製可能ウイルスの存在または非存在を評価するための方法および試薬 |
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-
2001
- 2001-08-10 JP JP2002519680A patent/JP5213297B2/ja not_active Expired - Lifetime
- 2001-08-10 CA CA2417986A patent/CA2417986C/en not_active Expired - Lifetime
- 2001-08-10 ES ES01959722T patent/ES2267803T3/es not_active Expired - Lifetime
- 2001-08-10 AT AT01959722T patent/ATE332398T1/de not_active IP Right Cessation
- 2001-08-10 WO PCT/US2001/025231 patent/WO2002014555A2/en not_active Ceased
- 2001-08-10 US US09/927,842 patent/US6635427B2/en not_active Expired - Lifetime
- 2001-08-10 DE DE60121342T patent/DE60121342T2/de not_active Expired - Lifetime
- 2001-08-10 EP EP01959722A patent/EP1307592B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ATE332398T1 (de) | 2006-07-15 |
| ES2267803T3 (es) | 2007-03-16 |
| CA2417986A1 (en) | 2002-02-21 |
| US6635427B2 (en) | 2003-10-21 |
| CA2417986C (en) | 2013-11-26 |
| JP2004506431A (ja) | 2004-03-04 |
| EP1307592B1 (en) | 2006-07-05 |
| DE60121342T2 (de) | 2007-06-28 |
| DE60121342D1 (de) | 2006-08-17 |
| EP1307592A2 (en) | 2003-05-07 |
| WO2002014555A2 (en) | 2002-02-21 |
| WO2002014555A3 (en) | 2003-02-27 |
| US20030022177A1 (en) | 2003-01-30 |
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