ATE510027T1 - Pcr-heissstart durch magnesium-sequestrierung - Google Patents
Pcr-heissstart durch magnesium-sequestrierungInfo
- Publication number
- ATE510027T1 ATE510027T1 AT07703556T AT07703556T ATE510027T1 AT E510027 T1 ATE510027 T1 AT E510027T1 AT 07703556 T AT07703556 T AT 07703556T AT 07703556 T AT07703556 T AT 07703556T AT E510027 T1 ATE510027 T1 AT E510027T1
- Authority
- AT
- Austria
- Prior art keywords
- hot start
- pcr hot
- sequestration
- magnesium
- present
- Prior art date
Links
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 title 1
- 229910052749 magnesium Inorganic materials 0.000 title 1
- 239000011777 magnesium Substances 0.000 title 1
- 230000009919 sequestration Effects 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Compounds Of Alkaline-Earth Elements, Aluminum Or Rare-Earth Metals (AREA)
- Oscillators With Electromechanical Resonators (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06003903 | 2006-02-27 | ||
| PCT/EP2007/001585 WO2007096182A1 (en) | 2006-02-27 | 2007-02-23 | Pcr hot start by magnesium sequestration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE510027T1 true ATE510027T1 (de) | 2011-06-15 |
Family
ID=36423585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07703556T ATE510027T1 (de) | 2006-02-27 | 2007-02-23 | Pcr-heissstart durch magnesium-sequestrierung |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8026058B2 (de) |
| EP (1) | EP1989324B1 (de) |
| JP (1) | JP5022383B2 (de) |
| CN (2) | CN103172700B (de) |
| AT (1) | ATE510027T1 (de) |
| CA (1) | CA2638854C (de) |
| ES (1) | ES2364983T3 (de) |
| HK (1) | HK1186742A1 (de) |
| WO (1) | WO2007096182A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| US7910720B2 (en) * | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| EP2436777B1 (de) | 2009-05-26 | 2015-08-19 | Xiamen University | Verfahren zur erkennung von variationen in nukleinsäuresequenzen |
| JP5911495B2 (ja) | 2010-10-04 | 2016-04-27 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 同じ反応容器内での細胞溶解およびｐｃｒのための方法 |
| EP2625286B1 (de) | 2010-10-04 | 2020-04-15 | Roche Diagniostics GmbH | Verfahren für zelllyse in einem pcr-reaktionspuffer |
| JP5798631B2 (ja) | 2010-10-04 | 2015-10-21 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法 |
| EP3778915A1 (de) * | 2012-06-08 | 2021-02-17 | Ionian Technologies, LLC | Nukleinsäureamplifizierungen |
| US10633697B2 (en) | 2014-01-31 | 2020-04-28 | Qiagen Gmbh | Caution chelator hot start |
| EP3164507B1 (de) | 2014-07-02 | 2020-02-12 | Promega Corporation | Umkehrbare metallionenchelatoren |
| CN105755119A (zh) * | 2016-03-14 | 2016-07-13 | 江西中大赛尔生物技术股份有限公司 | 一种pcr热启动方法 |
| CN106544340A (zh) * | 2016-12-08 | 2017-03-29 | 山西大学 | 一种适于高at含量dna的pcr扩增体系及方法 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5449603A (en) | 1989-10-24 | 1995-09-12 | Stratagene | Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein |
| KR950013953B1 (ko) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
| ES2141088T3 (es) | 1990-02-16 | 2000-03-16 | Hoffmann La Roche | Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa. |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB2293238A (en) | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| DE69534217T2 (de) | 1994-12-09 | 2006-01-12 | Wakunaga Seiyaku K.K. | Verfahren zur hemmung von nichtspezifischer hybridisierung in primerverlängerungsreaktionen |
| CA2168712A1 (en) * | 1995-02-07 | 1996-08-08 | John William Henderson Sutherland | Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction |
| CA2176193A1 (en) | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| DK2080813T3 (da) | 1995-06-07 | 2011-03-28 | Gilead Sciences Inc | Nukleinsyreligander, som binder til og inhiberer DNA-polymeraser |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US6416948B1 (en) | 1996-06-03 | 2002-07-09 | The Governors Of The University Of Alberta | Methods for detection of rearranged DNA |
| EP1179600B1 (de) | 1996-06-04 | 2005-05-11 | University Of Utah Research Foundation | Überwachung der Hybridisierung während PCR |
| DE69727932T2 (de) | 1996-06-04 | 2005-03-10 | University Of Utah Research Foundation, Salt Lake City | Vorrichtung und verfahren zur durchführung und überwachung von polymerase kettenreaktionen |
| US6617114B1 (en) * | 1996-10-31 | 2003-09-09 | Karo Bio Ab | Identification of drug complementary combinatorial libraries |
| EP0930370B1 (de) | 1997-12-15 | 2007-08-08 | CSL Behring GmbH | Markierter Primer, geeignet für die Detektion von Nukleinsäuren |
| US6787305B1 (en) | 1998-03-13 | 2004-09-07 | Invitrogen Corporation | Compositions and methods for enhanced synthesis of nucleic acid molecules |
| US6391551B1 (en) | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
| DE19913923A1 (de) | 1999-03-26 | 2000-09-28 | Amphenol Tuchel Elect | Kontaktiereinrichtung für eine SIM-Karte |
| JP2002543795A (ja) | 1999-05-12 | 2002-12-24 | インビトロゲン・コーポレーション | 核酸合成の感度および特異性の増大のための組成物および方法 |
| ATE332398T1 (de) | 2000-08-11 | 2006-07-15 | Univ Utah Res Found | Einfach markierte oligonukleotidsonden |
| US6746104B2 (en) * | 2000-09-25 | 2004-06-08 | Picoliter Inc. | Method for generating molecular arrays on porous surfaces |
| EP1275735A1 (de) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| US6403341B1 (en) * | 2001-08-02 | 2002-06-11 | Wayne M. Barnes | Magnesium precipitate hot start method for PCR |
| US6667165B2 (en) | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
-
2007
- 2007-02-23 WO PCT/EP2007/001585 patent/WO2007096182A1/en active Application Filing
- 2007-02-23 CA CA2638854A patent/CA2638854C/en not_active Expired - Fee Related
- 2007-02-23 CN CN201310067097.3A patent/CN103172700B/zh not_active Expired - Fee Related
- 2007-02-23 AT AT07703556T patent/ATE510027T1/de not_active IP Right Cessation
- 2007-02-23 EP EP07703556A patent/EP1989324B1/de not_active Not-in-force
- 2007-02-23 JP JP2008555707A patent/JP5022383B2/ja not_active Expired - Fee Related
- 2007-02-23 ES ES07703556T patent/ES2364983T3/es active Active
- 2007-02-23 CN CN200780006644.XA patent/CN101421417B/zh not_active Expired - Fee Related
-
2008
- 2008-08-27 US US12/198,966 patent/US8026058B2/en not_active Expired - Fee Related
-
2013
- 2013-12-20 HK HK13114137.0A patent/HK1186742A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2638854C (en) | 2012-05-15 |
| JP2009528283A (ja) | 2009-08-06 |
| ES2364983T3 (es) | 2011-09-20 |
| CN103172700A (zh) | 2013-06-26 |
| EP1989324A1 (de) | 2008-11-12 |
| CA2638854A1 (en) | 2007-08-30 |
| JP5022383B2 (ja) | 2012-09-12 |
| EP1989324B1 (de) | 2011-05-18 |
| CN103172700B (zh) | 2014-12-17 |
| CN101421417B (zh) | 2013-04-03 |
| CN101421417A (zh) | 2009-04-29 |
| US20100285535A1 (en) | 2010-11-11 |
| US8026058B2 (en) | 2011-09-27 |
| HK1186742A1 (en) | 2014-03-21 |
| WO2007096182A1 (en) | 2007-08-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |