JP2003516163A5 - - Google Patents

Download PDF

Info

Publication number
JP2003516163A5
JP2003516163A5 JP2001544376A JP2001544376A JP2003516163A5 JP 2003516163 A5 JP2003516163 A5 JP 2003516163A5 JP 2001544376 A JP2001544376 A JP 2001544376A JP 2001544376 A JP2001544376 A JP 2001544376A JP 2003516163 A5 JP2003516163 A5 JP 2003516163A5
Authority
JP
Japan
Prior art keywords
nucleic acid
linker
quencher
odn
moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2001544376A
Other languages
English (en)
Japanese (ja)
Other versions
JP5019688B2 (ja
JP2003516163A (ja
Filing date
Publication date
Priority claimed from US09/457,616 external-priority patent/US6727356B1/en
Application filed filed Critical
Publication of JP2003516163A publication Critical patent/JP2003516163A/ja
Publication of JP2003516163A5 publication Critical patent/JP2003516163A5/ja
Application granted granted Critical
Publication of JP5019688B2 publication Critical patent/JP5019688B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

JP2001544376A 1999-12-08 2000-12-08 蛍光消光検出試薬及び方法 Expired - Lifetime JP5019688B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/457,616 US6727356B1 (en) 1999-12-08 1999-12-08 Fluorescent quenching detection reagents and methods
US09/457,616 1999-12-08
PCT/US2000/033333 WO2001042505A2 (en) 1999-12-08 2000-12-08 Fluorescent quenching detection reagents and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012093428A Division JP2012175975A (ja) 1999-12-08 2012-04-16 蛍光消光検出試薬及び方法

Publications (3)

Publication Number Publication Date
JP2003516163A JP2003516163A (ja) 2003-05-13
JP2003516163A5 true JP2003516163A5 (https=) 2008-02-07
JP5019688B2 JP5019688B2 (ja) 2012-09-05

Family

ID=23817445

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2001544376A Expired - Lifetime JP5019688B2 (ja) 1999-12-08 2000-12-08 蛍光消光検出試薬及び方法
JP2012093428A Pending JP2012175975A (ja) 1999-12-08 2012-04-16 蛍光消光検出試薬及び方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2012093428A Pending JP2012175975A (ja) 1999-12-08 2012-04-16 蛍光消光検出試薬及び方法

Country Status (8)

Country Link
US (5) US6727356B1 (https=)
EP (1) EP1235938B1 (https=)
JP (2) JP5019688B2 (https=)
AT (1) ATE544771T1 (https=)
AU (1) AU782204B2 (https=)
CA (1) CA2396795A1 (https=)
ES (1) ES2381861T3 (https=)
WO (1) WO2001042505A2 (https=)

Families Citing this family (159)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7715989B2 (en) * 1998-04-03 2010-05-11 Elitech Holding B.V. Systems and methods for predicting oligonucleotide melting temperature (TmS)
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
EP1944310A3 (en) 2000-03-01 2008-08-06 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
EP1261616B1 (en) 2000-03-01 2010-08-18 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
CA2383939C (en) * 2000-06-27 2009-12-01 National Institute Of Advanced Industrial Science And Technology Novel nucleic acid probes, method for determining nucleic acids by using the probes, and method for analyzing data obtained by the method
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1317511B1 (de) * 2000-09-06 2005-01-19 Evotec OAI AG Oxazinderivate
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
US6902900B2 (en) * 2001-10-19 2005-06-07 Prolico, Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20040072195A1 (en) * 2002-01-25 2004-04-15 Hunkapiller Michael W. Single-tube, ready-to-use assay kits, and methods using same
EP1706826A4 (en) * 2002-01-25 2008-01-30 Applera Corp METHOD FOR TASKING, ACCEPTANCE AND PERFORMANCE OF ORDERS FOR PRODUCTS AND SERVICES
US20090239233A1 (en) * 2002-01-25 2009-09-24 Applera Corporation Single-tube, ready-to-use assay kits, and methods using same
JP4481656B2 (ja) * 2002-02-27 2010-06-16 ライフ テクノロジーズ コーポレーション ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物
US6818420B2 (en) * 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
AU2003213836A1 (en) * 2002-03-11 2003-09-29 Epoch Biosciences, Inc. Negatively charged minor groove binders
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
US7109316B2 (en) * 2002-08-23 2006-09-19 Ce Healthcare Bio-Sciences Corp. Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
CA2498320A1 (en) 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
EP1405920A3 (en) * 2002-10-02 2004-04-14 Roche Diagnostics GmbH Improved FRET process
CN100439515C (zh) * 2003-03-03 2008-12-03 清华大学 一种核酸分析芯片实验室系统与应用
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
WO2005003373A2 (en) * 2003-06-26 2005-01-13 Proligo, Llc Fluorogenic nucleic acid probes including lna for methods to detect and/or quantify nucleic acid analytes
US7381818B2 (en) * 2003-10-28 2008-06-03 Epoch Biosciences, Inc. Fluorescent probes containing 5′-minor groove binder, fluorophore and quenching moieties and methods of use thereof
US20050112671A1 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. FRET efficiency methods
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7618778B2 (en) 2004-06-02 2009-11-17 Kaufman Joseph C Producing, cataloging and classifying sequence tags
WO2006010038A2 (en) * 2004-07-08 2006-01-26 Biosearch Technologies, Inc. Inducible fluorescence assay
US7319022B1 (en) * 2004-08-13 2008-01-15 Epoch Biosciences, Inc. Amplification methods
US8183052B2 (en) * 2004-08-19 2012-05-22 Blood Cell Storage, Inc. Methods and apparatus for sterility testing
JP5017113B2 (ja) 2004-08-19 2012-09-05 ブラッド セル ストレージ， インコーポレイテッド 蛍光ｐＨ検出システムおよび関連する方法
EP1627926A1 (en) * 2004-08-19 2006-02-22 Roche Diagnostics GmbH A method to reduce false positive results
US8497134B2 (en) * 2004-08-19 2013-07-30 Blood Cell Storage, Inc. Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices
EP1789794A1 (en) * 2004-09-14 2007-05-30 Applera Corporation, Applied Biosystems Group Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
EP1844161A4 (en) * 2005-02-04 2010-10-27 Berry & Associates Inc DARK QUENCHERS, PROBES AND OTHER CONJUGATES WITH THEM AND THEIR USE
CA2599709A1 (en) * 2005-03-09 2006-09-21 Cepheid Polar dyes
HRP20100659T1 (hr) 2005-05-02 2011-01-31 Baseclick Gmbh Nove strategije obiljezavanja za osjetljivo otkrivanje analita
EP2270228B1 (en) * 2005-05-02 2013-08-21 baseclick GmbH New labelling strategies for the sensitive detection of analytes
US8632970B2 (en) * 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880206B1 (en) * 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex capture of nucleic acids
US7803541B2 (en) * 2005-05-12 2010-09-28 Panomics, Inc. Multiplex branched-chain DNA assays
JP2008545659A (ja) 2005-05-20 2008-12-18 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド オリゴヌクレオチドを標識する化合物及び方法
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
DK2500439T4 (da) * 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
DE102005029811B4 (de) * 2005-06-27 2009-03-12 Siemens Ag Oligonukleotidanordnungen, Verfahren zu deren Einsatz und deren Verwendung
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
US20100016208A1 (en) * 2005-11-15 2010-01-21 The General Hospital Corporation Photoactivatable antimicrobial agents
US7345303B2 (en) * 2005-12-22 2008-03-18 Xerox Corporation Organic thin-film transistors
US20070218490A1 (en) * 2006-03-15 2007-09-20 Integrated Dna Technologies, Inc. Nucleic acid monomers with 2'-chemical moieties
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
ATE515488T1 (de) 2006-10-31 2011-07-15 Baseclick Gmbh Click-chemie zur herstellung von reportermolekülen
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US7960139B2 (en) 2007-03-23 2011-06-14 Academia Sinica Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells
EP2147009A4 (en) * 2007-05-18 2014-01-08 Dharmacon Inc NOVEL CHROMOPHORE SILYL PROTECTION GROUPS AND THEIR USE IN THE CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES
US8084589B2 (en) * 2007-08-31 2011-12-27 University Of Massachusetts Phosphoramidite nucleoside analogs
JP4557014B2 (ja) * 2008-02-14 2010-10-06 ソニー株式会社 核酸検出用蛍光標識オリゴヌクレオチド、及び該核酸検出用蛍光標識オリゴヌクレオチドを用いた二本鎖形成に関わる情報を得る方法
WO2009124150A2 (en) * 2008-04-01 2009-10-08 Biosearch Technologies, Inc. Stabilized nucleic acid dark quencher-fluorophore probes
ES2699376T3 (es) 2008-05-05 2019-02-11 Massachusetts Gen Hospital Métodos para usar sustratos enzimáticos marcados con bodipy
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
ES2442024T3 (es) 2008-07-15 2014-02-07 Academia Sinica Matrices de glucano sobre portaobjetos de vidrio revestidos con aluminio de tipo PTFE y métodos relacionados
US9657338B2 (en) 2008-10-01 2017-05-23 Koninklijke Philips N.V. Method for immobilizing nucleic acids on a support
EP2334808B1 (en) * 2008-10-01 2017-11-15 Koninklijke Philips N.V. Method for testing and quality controlling of nucleic acids on a support
EP2376659B1 (en) * 2008-12-17 2015-12-02 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
EP2411543B1 (en) 2009-03-27 2017-04-19 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
EP2478013B1 (en) 2009-09-16 2018-10-24 F.Hoffmann-La Roche Ag Coiled coil and/or tether containing protein complexes and uses thereof
US10087236B2 (en) 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
US11377485B2 (en) 2009-12-02 2022-07-05 Academia Sinica Methods for modifying human antibodies by glycan engineering
US20110151457A1 (en) 2009-12-22 2011-06-23 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2553123B1 (en) 2010-03-26 2016-08-24 Integrated DNA Technologies, Inc. Methods for enhancing nucleic acid hybridization
TW201138821A (en) 2010-03-26 2011-11-16 Roche Glycart Ag Bispecific antibodies
US10338069B2 (en) 2010-04-12 2019-07-02 Academia Sinica Glycan arrays for high throughput screening of viruses
DK3109326T3 (da) 2010-06-21 2022-02-14 Life Technologies Corp Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt
EP2582850B1 (en) 2010-06-21 2016-11-09 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
AU2011299233B2 (en) 2010-09-07 2016-09-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US8658361B2 (en) 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
KR20120042100A (ko) * 2010-10-22 2012-05-03 주식회사 씨젠 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출
WO2012085064A1 (en) 2010-12-23 2012-06-28 Roche Diagnostics Gmbh Detection of a posttranslationally modified polypeptide by a bi-valent binding agent
SG191153A1 (en) 2010-12-23 2013-07-31 Hoffmann La Roche Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
WO2012129547A1 (en) 2011-03-23 2012-09-27 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
ES2537189T3 (es) 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
US9040307B2 (en) 2011-05-27 2015-05-26 Blood Cell Storage, Inc. Fluorescent pH detector system and related methods
CN103906848B (zh) 2011-08-18 2016-03-02 雀巢产品技术援助有限公司 用于检测等位变体的组合物和方法
CA2861124A1 (en) 2012-02-10 2013-08-15 Genentech, Inc. Single-chain antibodies and other heteromultimers
RS55868B1 (sr) 2012-03-22 2017-08-31 Lgc Genomics Ltd Sistem za detekciju lančane reakcije polimeraze pomoću oligonukleotida koji sadrže fosforotioatnu grupu
US10130714B2 (en) 2012-04-14 2018-11-20 Academia Sinica Enhanced anti-influenza agents conjugated with anti-inflammatory activity
WO2013192521A1 (en) 2012-06-22 2013-12-27 The General Hospital Corporation β-LACTAMASE TARGETED PHOTOSENSITIZER FOR PESTICIDE AND PEST DETECTION
KR20150030744A (ko) 2012-06-27 2015-03-20 에프. 호프만-라 로슈 아게 표적에 특이적으로 결합하는 하나 이상의 결합 단위를 포함하는 항체 Ｆｃ－영역 접합체의 제조 방법 및 그의 용도
RU2639287C2 (ru) 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения
WO2014031498A1 (en) 2012-08-18 2014-02-27 Academia Sinica Cell-permeable probes for identification and imaging of sialidases
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2014164874A2 (en) 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
WO2014159063A1 (en) 2013-03-14 2014-10-02 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
SG11201508185YA (en) * 2013-04-19 2015-11-27 Agency Science Tech & Res Tunable fluorescence using cleavable linkers
US9328384B2 (en) 2013-05-13 2016-05-03 Elitechgroup B.V. Droplet digital PCR with short minor groove probes
EP3013365B1 (en) 2013-06-26 2019-06-05 Academia Sinica Rm2 antigens and use thereof
EP3013347B1 (en) 2013-06-27 2019-12-11 Academia Sinica Glycan conjugates and use thereof
JP6486368B2 (ja) 2013-09-06 2019-03-20 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 改変されたグリコシル基を含む糖脂質を用いたヒトｉＮＫＴ細胞の活性化
AU2015206370A1 (en) 2014-01-16 2016-07-07 Academia Sinica Compositions and methods for treatment and detection of cancers
US10150818B2 (en) 2014-01-16 2018-12-11 Academia Sinica Compositions and methods for treatment and detection of cancers
CN106415244B (zh) 2014-03-27 2020-04-24 中央研究院 反应性标记化合物及其用途
JP6681841B2 (ja) 2014-05-09 2020-04-15 ライフコーデックス アーゲー 特別な細胞タイプに由来するｄｎａの検出とそれに関連する方法
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
JP7093612B2 (ja) 2014-05-27 2022-06-30 アカデミア シニカ Ｂａｃｔｅｒｏｉｄｅｓ由来のフコシダーゼおよびそれを使用する方法
US10118969B2 (en) 2014-05-27 2018-11-06 Academia Sinica Compositions and methods relating to universal glycoforms for enhanced antibody efficacy
CN106661099A (zh) 2014-05-27 2017-05-10 中央研究院 抗her2醣抗体及其用途
CA2950415A1 (en) 2014-05-27 2015-12-03 Academia Sinica Anti-cd20 glycoantibodies and uses thereof
WO2015184001A1 (en) 2014-05-28 2015-12-03 Academia Sinica Anti-tnf-alpha glycoantibodies and uses thereof
TWI745275B (zh) 2014-09-08 2021-11-11 中央研究院 使用醣脂激活人類ｉＮＫＴ細胞
EP3227332B1 (en) 2014-12-03 2019-11-06 F.Hoffmann-La Roche Ag Multispecific antibodies
US9988670B2 (en) 2014-12-12 2018-06-05 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
EP3230467A1 (en) 2014-12-12 2017-10-18 ELITechGroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
US10495645B2 (en) 2015-01-16 2019-12-03 Academia Sinica Cancer markers and methods of use thereof
US9975965B2 (en) 2015-01-16 2018-05-22 Academia Sinica Compositions and methods for treatment and detection of cancers
JP6814139B2 (ja) * 2015-01-20 2021-01-13 バイオサーチ テクノロジーズ インコーポレイテッド クマリン系化合物及び関連する方法
JP6779887B2 (ja) 2015-01-24 2020-11-04 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ 新規なグリカンコンジュゲートおよびその使用方法
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
EP3362462B1 (en) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
WO2017123647A1 (en) 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
EP3426693A4 (en) 2016-03-08 2019-11-13 Academia Sinica PROCESS FOR MODULAR SYNTHESIS OF N-GLYCANES AND ARRANGEMENTS THEREOF
WO2017177025A1 (en) 2016-04-06 2017-10-12 Life Technologies Corporation Compositions, methods, and kits for synthesis and detection of nucleic acids
US11155885B2 (en) 2016-05-20 2021-10-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Real-time reverse transcriptase-polymerase chain reaction assay with modified probe for the diagnosis of rabies viruses and other lyssaviruses
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates
CA3031812A1 (en) 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
CA3034057A1 (en) 2016-08-22 2018-03-01 CHO Pharma Inc. Antibodies, binding fragments, and methods of use
EP4556575A3 (en) 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
EP3592757A2 (en) 2017-03-09 2020-01-15 ELITechGroup, Inc. Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes
EP3668884B1 (en) * 2017-08-17 2022-03-09 ELITechGroup Inc. Duplex stabilizing fluorescence quenchers for nucleic acid probes
GB201713251D0 (en) 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
US12071547B2 (en) 2018-05-07 2024-08-27 Cepheid Sulforhodamine phosphoramidite dyes
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
WO2019231617A1 (en) 2018-05-29 2019-12-05 Elitechgroup, Inc. Carborhodamine compounds and methods of preparation thereof
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
WO2020037290A1 (en) 2018-08-16 2020-02-20 Life Technologies Corporation Reagents, mixtures, kits and methods for amplification of nucleic acids
US12275964B2 (en) 2018-08-22 2025-04-15 The Regents Of The University Of California Variant type V CRISPR/Cas effector polypeptides and methods of use thereof
US20210317527A1 (en) 2018-08-27 2021-10-14 The Regents Of The University Of California Reporter nucleic acids for type v crispr-mediated detection
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
MX2021010559A (es) 2019-03-07 2021-12-15 Univ California Polipéptidos efectores de crispr-cas y métodos de uso de estos.
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021240443A1 (en) 2020-05-27 2021-12-02 King Abdullah University Of Science And Technology Iscan: an rt-lamp-coupled crispr-cas module for rapid, sensitive detection of sars-cov-2
AU2021307437A1 (en) 2020-07-16 2023-03-16 Cepheid Fluorescent dyes
WO2022020731A2 (en) 2020-07-23 2022-01-27 Life Technologies Corporation Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
EP4185596A1 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
US20250092261A1 (en) 2021-07-21 2025-03-20 Life Technologies Corporation Dibenzoxanthene Quenchers, Uses, and Methods of Preparation
WO2023023673A2 (en) 2021-08-20 2023-02-23 Kasa Bio, L.L.C. Compositions and methods for multiplex detection of mirna and other polynucelotides
WO2025007089A1 (en) 2023-06-30 2025-01-02 Life Technologies Corporation Compositions, methods and kits for nucleic acids synthesis and amplification
WO2025151807A1 (en) 2024-01-11 2025-07-17 Life Technologies Corporation Crude lysate sample extraction for digital pcr
WO2025175243A1 (en) 2024-02-15 2025-08-21 Life Technologies Corporation Amplification and data collection protocol for rapid genotyping
WO2025175212A1 (en) 2024-02-15 2025-08-21 Life Technologies Corporation Methods for rapid extraction-free genotyping
CN121554397A (zh) * 2025-11-28 2026-02-24 北京海精高创科技有限公司 偶氮苯类淬灭单元、固相合成载体及其制备方法、核酸探针

Family Cites Families (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US952202A (en) * 1909-05-22 1910-03-15 Heinrich Klingenfeld Combined garment support and form.
US2264303A (en) 1940-03-27 1941-12-02 Eastman Kodak Co Azo compounds and material colored therewith
US3407189A (en) 1956-07-25 1968-10-22 Sandoz Ltd Water-insoluble monoazo dyestuffs
GB1394368A (en) 1972-12-14 1975-05-14 Ici Ltd Disperse azo dyestuffs
US3970617A (en) * 1974-03-07 1976-07-20 E. I. Du Pont De Nemours And Company Navy-blue nitrophenylazonaphthylamino dyes
GB1533121A (en) 1975-08-04 1978-11-22 Ici Ltd Disperse nitro disazo dyestuffs
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US4570542A (en) * 1982-05-17 1986-02-18 Weld Tooling Corporation Ribbon rail systems
DE3526565A1 (de) 1985-07-25 1987-02-05 Boehringer Mannheim Gmbh Resorufin-derivate, verfahren zu deren herstellung sowie deren verwendung in fluoreszenzimmunoassays
FR2588495B1 (fr) * 1985-10-16 1987-12-11 Aerospatiale Ensemble de douille de fixation pour unite d'usinage et unite d'usinage adaptee pour cooperer avec ladite douille
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5824796A (en) 1988-09-28 1998-10-20 Epoch Pharmaceuticals, Inc. Cross-linking oligonucleotides
US4850763A (en) * 1988-10-03 1989-07-25 The Boeing Company Tool track for use on aircraft
US5272259A (en) * 1988-12-23 1993-12-21 Bayer Aktiengesellschaft Stilbene dyestuffs and light-polarizing films or sheets containing stilbene
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
DE3916595A1 (de) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
US5451463A (en) 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5512667A (en) 1990-08-28 1996-04-30 Reed; Michael W. Trifunctional intermediates for preparing 3'-tailed oligonucleotides
EP0834575B1 (en) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identification of nucleic acids in samples
JPH04279556A (ja) * 1991-03-07 1992-10-05 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
EP0528512A1 (en) * 1991-08-20 1993-02-24 Pioneer Electronic Corporation Recording medium
JPH0578300A (ja) * 1991-09-19 1993-03-30 Nippon Oil & Fats Co Ltd フルオロアルキル基含有アゾ化合物及びその製造方法
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5502177A (en) * 1993-09-17 1996-03-26 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5395187A (en) * 1993-11-22 1995-03-07 Mcdonnell Douglas Corporation Method and apparatus for attaching a drill motor to a drill plate with a clamping device having an expandable collet
JPH07179773A (ja) * 1993-12-01 1995-07-18 Dainichiseika Color & Chem Mfg Co Ltd アゾ化合物
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US5482411A (en) * 1994-12-22 1996-01-09 Mcdonnell Douglas Corporation Method and apparatus for securely clamping a drill motor to a drill plate
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
GB9602265D0 (en) 1996-02-05 1996-04-03 Amersham Int Plc Benzophenoxazine dyes
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6036409A (en) * 1997-01-23 2000-03-14 The Boeing Company Blind hole self-collet countersink
US5846726A (en) 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6465175B2 (en) 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6007992A (en) 1997-11-10 1999-12-28 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US6028183A (en) 1997-11-07 2000-02-22 Gilead Sciences, Inc. Pyrimidine derivatives and oligonucleotides containing same
US6210084B1 (en) * 1997-11-26 2001-04-03 The Boeing Company Pressure foot assembly for clamping a joint
CA2318880C (en) 1998-02-04 2010-07-27 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
US6220099B1 (en) * 1998-02-17 2001-04-24 Ce Nuclear Power Llc Apparatus and method for performing non-destructive inspections of large area aircraft structures
US6073328A (en) * 1998-02-28 2000-06-13 Hendricks; James Carpet installation tool
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US6117986A (en) 1998-06-10 2000-09-12 Intergen Company, L.P. Pyrimidines linked to a quencher
US6240322B1 (en) * 1998-11-04 2001-05-29 Cardiac Pacemakers, Inc. System and apparatus having low profile collapsible tines
WO2000064988A1 (en) 1999-04-23 2000-11-02 Molecular Probes, Inc. Xanthene dyes and their application as luminescence quenching compounds
US6151241A (en) 1999-05-19 2000-11-21 Symetrix Corporation Ferroelectric memory with disturb protection
US6114518A (en) 1999-09-30 2000-09-05 Becton, Dickinson And Company Synthesis and use of labelled phosphoramidite compositions
US6467385B1 (en) * 1999-12-03 2002-10-22 The Boeing Company Panel trimming system
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6465644B1 (en) 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
US6430796B1 (en) * 2000-05-03 2002-08-13 The Boeing Company Apparatus for performing automated manufacturing operations on panel-shaped workpieces
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6550129B1 (en) * 2000-09-25 2003-04-22 The Boeing Company Portable computer numeric controlled manufacturing machines and associated methods
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis

Similar Documents

Publication Publication Date Title
JP2003516163A5 (https=)
US11028121B2 (en) Multisignal labeling reagents and processes and uses therefor
US5912340A (en) Selective binding complementary oligonucleotides
US7851606B2 (en) Negatively charged minor groove binders
JP3172178B2 (ja) 合成オリゴヌクレオチドに対する多重リポータ基の組込み
JP2001512131A (ja) 塩基アナログ
WO1990014353A1 (en) Crosslinking oligonucleotides
JPH10504719A (ja) 非ヌクレオチド部分を有するポリヌクレオチド試薬、ならびに合成および使用に関連する方法
JP2003508065A (ja) Ｕｖ励起性蛍光エネルギー移動色素
US20080038745A1 (en) Nucleotide analogs with six-membered rings
JP2004305216A (ja) 核酸オリゴマー、その製造方法及びそれを製造するためのキット
Korshun et al. New pyrene derivatives for fluorescent labeling of oligonucleotides
JP2004502701A (ja) 塩基類似体
JP2003528883A (ja) オリゴヌクレオチド・ハイブリダイゼーションにユニバーサル・ヌクレオシドとして使用されるｎ８−及びｃ８−連結プリン塩基、並びに構造的に関連したヘテロ環
WO2004018712A1 (en) OLIGONUCLEOTIDE TAGGED NUCLEOSIDE TRIPHOSPHATES (OTNTPs) FOR GENETIC ANALYSIS
WO2009058852A2 (en) Minor groove binder-energy transfer oligonucleotides and methods for their use
JP5467275B2 (ja) 電子移動抑制剤とその利用
US20060063147A1 (en) Omega-amino-PEG-phosphoramidites and conjugates thereof
JP2002529473A (ja) ポリヌクレオチド架橋成分
EP0334694A1 (fr) Sonde d'acides nucléiques avec marquage non-radioactif et procédés de préparation
US6534646B2 (en) Oligonucleotide labeling reagents
EP0616612A1 (en) Nuclease stable and binding competent oligomers and methods for their use
US20020015953A1 (en) Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof