JP2002090628A - 共焦点顕微鏡 - Google Patents
共焦点顕微鏡Info
- Publication number
- JP2002090628A JP2002090628A JP2000282695A JP2000282695A JP2002090628A JP 2002090628 A JP2002090628 A JP 2002090628A JP 2000282695 A JP2000282695 A JP 2000282695A JP 2000282695 A JP2000282695 A JP 2000282695A JP 2002090628 A JP2002090628 A JP 2002090628A
- Authority
- JP
- Japan
- Prior art keywords
- light
- micro
- mirror
- fluorescence
- minute
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000011159 matrix material Substances 0.000 claims abstract description 9
- 230000005284 excitation Effects 0.000 claims description 146
- 239000007850 fluorescent dye Substances 0.000 claims description 101
- 230000003287 optical effect Effects 0.000 claims description 97
- 238000006073 displacement reaction Methods 0.000 claims description 10
- 230000001360 synchronised effect Effects 0.000 claims description 6
- 230000009471 action Effects 0.000 abstract description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 53
- 238000001514 detection method Methods 0.000 description 30
- 230000003595 spectral effect Effects 0.000 description 27
- 230000004888 barrier function Effects 0.000 description 23
- 210000001747 pupil Anatomy 0.000 description 23
- 238000003384 imaging method Methods 0.000 description 21
- 230000006870 function Effects 0.000 description 19
- 238000012937 correction Methods 0.000 description 17
- 230000007246 mechanism Effects 0.000 description 17
- 239000000835 fiber Substances 0.000 description 16
- 238000010586 diagram Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 238000005286 illumination Methods 0.000 description 10
- 239000004973 liquid crystal related substance Substances 0.000 description 6
- 230000005540 biological transmission Effects 0.000 description 5
- 239000004065 semiconductor Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000009347 mechanical transmission Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
Landscapes
- Microscoopes, Condenser (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000282695A JP2002090628A (ja) | 2000-09-18 | 2000-09-18 | 共焦点顕微鏡 |
| US09/877,767 US6433929B1 (en) | 2000-06-12 | 2001-06-08 | Scanning optical microscope and method of acquiring image |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000282695A JP2002090628A (ja) | 2000-09-18 | 2000-09-18 | 共焦点顕微鏡 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002090628A true JP2002090628A (ja) | 2002-03-27 |
| JP2002090628A5 JP2002090628A5 (enrdf_load_stackoverflow) | 2007-11-01 |
Family
ID=18767163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000282695A Pending JP2002090628A (ja) | 2000-06-12 | 2000-09-18 | 共焦点顕微鏡 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2002090628A (enrdf_load_stackoverflow) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1580586A1 (en) * | 2004-03-25 | 2005-09-28 | Olympus Corporation | Scanning confocal microscope |
| JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
| JP2006503283A (ja) * | 2002-10-16 | 2006-01-26 | パーキンエルマー・シンガポール・プライベート・リミテッド | 画像化に関する改善 |
| JP2007171598A (ja) * | 2005-12-22 | 2007-07-05 | Olympus Corp | 共焦点顕微鏡 |
| JP2008249669A (ja) * | 2007-03-30 | 2008-10-16 | Osaka Prefecture Univ | 物質・物体の認識・識別方法および装置、蛍光顕微鏡、ヘッドアップディスプレイ装置 |
| JP2014170045A (ja) * | 2013-03-01 | 2014-09-18 | Olympus Corp | 走査型レーザ顕微鏡装置 |
| CN109745008A (zh) * | 2019-01-31 | 2019-05-14 | 北京超维景生物科技有限公司 | 可吸附式显微镜探测装置及激光扫描显微镜 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05501458A (ja) * | 1990-08-10 | 1993-03-18 | リージェンツ・オブ・ザ・ユニバーシティー・オブ・ミネソタ | 共焦点顕微鏡用レーザ |
| JPH07333510A (ja) * | 1994-06-02 | 1995-12-22 | Nikon Corp | レーザ走査顕微鏡装置 |
| JP2000056244A (ja) * | 1998-08-04 | 2000-02-25 | Carl Zeiss Jena Gmbh | レ―ザ走査顕微鏡および照明およびまたは検出装置 |
| JP2000199855A (ja) * | 1998-11-02 | 2000-07-18 | Olympus Optical Co Ltd | 走査型光学顕微鏡装置 |
-
2000
- 2000-09-18 JP JP2000282695A patent/JP2002090628A/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05501458A (ja) * | 1990-08-10 | 1993-03-18 | リージェンツ・オブ・ザ・ユニバーシティー・オブ・ミネソタ | 共焦点顕微鏡用レーザ |
| JPH07333510A (ja) * | 1994-06-02 | 1995-12-22 | Nikon Corp | レーザ走査顕微鏡装置 |
| JP2000056244A (ja) * | 1998-08-04 | 2000-02-25 | Carl Zeiss Jena Gmbh | レ―ザ走査顕微鏡および照明およびまたは検出装置 |
| JP2000199855A (ja) * | 1998-11-02 | 2000-07-18 | Olympus Optical Co Ltd | 走査型光学顕微鏡装置 |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006503283A (ja) * | 2002-10-16 | 2006-01-26 | パーキンエルマー・シンガポール・プライベート・リミテッド | 画像化に関する改善 |
| JP4829499B2 (ja) * | 2002-10-16 | 2011-12-07 | パーキンエルマー・シンガポール・プライベート・リミテッド | 画像化に関する改善 |
| US8289620B2 (en) | 2002-10-16 | 2012-10-16 | Perkinelmer Singapore Pte Ltd. | Imaging |
| EP1580586A1 (en) * | 2004-03-25 | 2005-09-28 | Olympus Corporation | Scanning confocal microscope |
| US7015444B2 (en) | 2004-03-25 | 2006-03-21 | Olympus Corporation | Optical-scanning examination apparatus |
| JP2005352030A (ja) * | 2004-06-09 | 2005-12-22 | Olympus Corp | 走査型レーザ観察装置、観察方法及び観察プログラム |
| JP2007171598A (ja) * | 2005-12-22 | 2007-07-05 | Olympus Corp | 共焦点顕微鏡 |
| JP2008249669A (ja) * | 2007-03-30 | 2008-10-16 | Osaka Prefecture Univ | 物質・物体の認識・識別方法および装置、蛍光顕微鏡、ヘッドアップディスプレイ装置 |
| JP2014170045A (ja) * | 2013-03-01 | 2014-09-18 | Olympus Corp | 走査型レーザ顕微鏡装置 |
| CN109745008A (zh) * | 2019-01-31 | 2019-05-14 | 北京超维景生物科技有限公司 | 可吸附式显微镜探测装置及激光扫描显微镜 |
| CN109745008B (zh) * | 2019-01-31 | 2024-05-14 | 北京超维景生物科技有限公司 | 可吸附式显微镜探测装置及激光扫描显微镜 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070913 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070913 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20100831 |
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| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110215 |
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| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20110719 |