IL136158A - שיטה לייצור תביעות אצבע מורכבות לאתרים ב- dna שעברו מתילציה - Google Patents
שיטה לייצור תביעות אצבע מורכבות לאתרים ב- dna שעברו מתילציהInfo
- Publication number
- IL136158A IL136158A IL13615898A IL13615898A IL136158A IL 136158 A IL136158 A IL 136158A IL 13615898 A IL13615898 A IL 13615898A IL 13615898 A IL13615898 A IL 13615898A IL 136158 A IL136158 A IL 136158A
- Authority
- IL
- Israel
- Prior art keywords
- dna
- oligonucleotides
- bisulfite
- reaction
- treated
- Prior art date
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19754482A DE19754482A1 (de) | 1997-11-27 | 1997-11-27 | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| PCT/DE1998/003558 WO1999028498A2 (de) | 1997-11-27 | 1998-11-27 | Verfahren zur herstellung komplexer dna-methylierungs-fingerabdrücke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL136158A0 IL136158A0 (en) | 2001-05-20 |
| IL136158A true IL136158A (he) | 2004-07-25 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL13615898A IL136158A (he) | 1997-11-27 | 1998-11-27 | שיטה לייצור תביעות אצבע מורכבות לאתרים ב- dna שעברו מתילציה |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US6214556B1 (he) |
| EP (1) | EP1034309B1 (he) |
| JP (1) | JP2001525181A (he) |
| CN (1) | CN1253582C (he) |
| AT (1) | ATE217348T1 (he) |
| AU (1) | AU753368B2 (he) |
| CA (1) | CA2310384C (he) |
| CZ (1) | CZ293278B6 (he) |
| DE (2) | DE19754482A1 (he) |
| DK (1) | DK1034309T3 (he) |
| ES (1) | ES2173669T3 (he) |
| HK (1) | HK1033473A1 (he) |
| HU (1) | HUP0100424A3 (he) |
| IL (1) | IL136158A (he) |
| IS (1) | IS1921B (he) |
| NZ (1) | NZ504586A (he) |
| PL (1) | PL341681A1 (he) |
| PT (1) | PT1034309E (he) |
| RU (1) | RU2223324C2 (he) |
| WO (1) | WO1999028498A2 (he) |
Families Citing this family (199)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6979728B2 (en) * | 1998-05-04 | 2005-12-27 | Baylor College Of Medicine | Articles of manufacture and methods for array based analysis of biological molecules |
| DE19853398C1 (de) * | 1998-11-19 | 2000-03-16 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA |
| WO2001001218A2 (en) * | 1999-06-25 | 2001-01-04 | Genaissance Pharmaceuticals, Inc. | Methods for obtaining and using haplotype data |
| US7058517B1 (en) | 1999-06-25 | 2006-06-06 | Genaissance Pharmaceuticals, Inc. | Methods for obtaining and using haplotype data |
| DE19935772C2 (de) * | 1999-07-26 | 2002-11-07 | Epigenomics Ag | Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben |
| DE19935749C2 (de) * | 1999-07-28 | 2003-06-26 | Epigenomics Ag | Verfahren zur Chrakterisierung von Nukleinsäurefragmenten |
| EP1261932B1 (en) * | 1999-10-13 | 2009-09-30 | Sequenom, Inc. | Methods for identifying polymorphic genetic markers |
| US20030190644A1 (en) | 1999-10-13 | 2003-10-09 | Andreas Braun | Methods for generating databases and databases for identifying polymorphic genetic markers |
| DE19951189C2 (de) * | 1999-10-15 | 2003-11-06 | Epigenomics Ag | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
| DE50007314D1 (de) * | 1999-11-12 | 2004-09-09 | Epigenomics Ag | Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen |
| DE19957827C2 (de) | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| DE19959691A1 (de) * | 1999-12-06 | 2001-08-16 | Epigenomics Ag | Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA |
| WO2001044634A1 (fr) * | 1999-12-15 | 2001-06-21 | Komatsu Zenoah Co. | Moteur deux temps de balayage stratifie a pistons |
| GB9929720D0 (en) * | 1999-12-17 | 2000-02-09 | Zeneca Ltd | Diagnostic method |
| DE19963536C2 (de) * | 1999-12-20 | 2003-04-10 | Epigenomics Ag | Verfahren zur Analyse von Nukleinsäuresequenzen |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US8076063B2 (en) * | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) * | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| DE10010280B4 (de) * | 2000-02-25 | 2006-08-10 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10010281B4 (de) * | 2000-02-25 | 2005-03-10 | Epigenomics Ag | Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10010282B4 (de) * | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| JP2004507213A (ja) * | 2000-03-15 | 2004-03-11 | エピゲノミクス アーゲー | 細胞周期に関連する疾患の診断 |
| DE10019058A1 (de) * | 2000-04-06 | 2001-12-20 | Epigenomics Ag | Detektion von Variationen des DNA-Methylierungsprofils |
| DE10013847A1 (de) * | 2000-03-15 | 2001-09-27 | Epigenomics Ag | Oligonukleotide oder PNA-Oligomere und Verfahren zur parallelen Detektion des Methylierungszustandes genomischer DNA |
| DE10032529A1 (de) * | 2000-06-30 | 2002-02-07 | Epigenomics Ag | Diagnose von bedeutenden genetischen Parametern innerhalb des Major Histocompatibility Complex (MHC) |
| AU2001278420A1 (en) | 2000-04-06 | 2001-11-07 | Epigenomics Ag | Diagnosis of diseases associated with dna repair |
| US20040241651A1 (en) * | 2000-04-07 | 2004-12-02 | Alexander Olek | Detection of single nucleotide polymorphisms (snp's) and cytosine-methylations |
| DE10029915B4 (de) * | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10347399B4 (de) * | 2003-10-09 | 2005-09-15 | Epigenomics Ag | Bisulfit-Umwandlung zum Nachweis von Cytosin-Methylierungen in DNA mittels optimierter Aufreinigung |
| DE10347400B4 (de) * | 2003-10-09 | 2005-08-04 | Epigenomics Ag | Verbesserte Bisulfit-Umwandlung von DNA durch kurzzeitige Temperaturerhöhungen |
| DE10029914A1 (de) * | 2000-06-19 | 2002-01-03 | Epigenomics Ag | Verfahren zur hochparallelen Analyse von Polymorphismen |
| US20060134643A1 (en) * | 2000-06-19 | 2006-06-22 | Kurt Berlin | Bisulfite conversion of DNA |
| DE10347396B4 (de) * | 2003-10-09 | 2005-06-23 | Epigenomics Ag | Optimierte Bisulfit-Umwandlung von DNA durch Einsatz von Dioxan |
| DE10347397B4 (de) * | 2003-10-09 | 2005-08-04 | Epigenomics Ag | Optimierte Bisulfit-Umwandlung durch Zusatz von n-Alkylenglykol-Verbindungen |
| US6931326B1 (en) | 2000-06-26 | 2005-08-16 | Genaissance Pharmaceuticals, Inc. | Methods for obtaining and using haplotype data |
| EP1294947A2 (en) * | 2000-06-30 | 2003-03-26 | Epigenomics AG | Method and nucleic acids for pharmacogenomic methylation analysis |
| CA2420840A1 (en) * | 2000-09-01 | 2003-02-27 | Epigenomics Ag | Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3' |
| DE10044543C2 (de) * | 2000-09-05 | 2003-09-11 | Epigenomics Ag | Verfahren zur Bestimmung des Methylierungsgrades von bestimmten Cytosinen in genomischer DNA im Sequenzkontext 5'-CpG-3' |
| WO2002018631A2 (de) * | 2000-09-01 | 2002-03-07 | Epigenomics Ag | Diagnose von bestehenden erkrankungen oder der prädisposition für bestimmte erkrankungen |
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| DE10054974A1 (de) * | 2000-11-06 | 2002-06-06 | Epigenomics Ag | Diagnose von mit Cdk4 assoziierten Krankheiten |
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| DE10061348C2 (de) * | 2000-12-06 | 2002-10-24 | Epigenomics Ag | Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA |
| DE10061338A1 (de) * | 2000-12-06 | 2002-06-20 | Epigenomics Ag | Diagnose von mit Angiogenese assoziierten Krankheiten |
| JP2002171973A (ja) * | 2000-12-07 | 2002-06-18 | Univ Tokyo | Ｄｎａメチル化パターンによる細胞の同定法 |
| DE10065815A1 (de) * | 2000-12-22 | 2002-07-11 | Epigenomics Ag | Verfahren zur flexiblen Herstellung von Oligomer-Arrays |
| DE10065814B4 (de) * | 2000-12-22 | 2004-07-08 | Epigenomics Ag | Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung |
| DE10104937B4 (de) * | 2001-01-29 | 2005-03-17 | Epigenomics Ag | Fluoreszenzpolarisation 2 |
| DE10104938B4 (de) * | 2001-01-29 | 2005-06-23 | Epigenomics Ag | Fluoreszenzpolarisation 1 |
| US6893820B1 (en) * | 2001-01-31 | 2005-05-17 | The Ohio State University Research Foundation | Detection of methylated CpG rich sequences diagnostic for malignant cells |
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| JPH09506253A (ja) * | 1993-11-30 | 1997-06-24 | マクギル・ユニヴァーシティ | Ｄｎａメチルトランスフェラーゼの阻害 |
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| US6017704A (en) * | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
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| IS5493A (is) | 2000-05-12 |
| CZ20001934A3 (cs) | 2000-11-15 |
| WO1999028498A3 (de) | 1999-09-02 |
| IL136158A0 (en) | 2001-05-20 |
| ATE217348T1 (de) | 2002-05-15 |
| DE59804090D1 (de) | 2002-06-13 |
| CA2310384A1 (en) | 1999-06-10 |
| AU753368B2 (en) | 2002-10-17 |
| HK1033473A1 (en) | 2001-08-31 |
| AU2408599A (en) | 1999-06-16 |
| PL341681A1 (en) | 2001-04-23 |
| DK1034309T3 (da) | 2002-08-26 |
| CN1253582C (zh) | 2006-04-26 |
| WO1999028498A2 (de) | 1999-06-10 |
| CZ293278B6 (cs) | 2004-03-17 |
| HUP0100424A3 (en) | 2003-08-28 |
| NZ504586A (en) | 2002-10-25 |
| CN1283235A (zh) | 2001-02-07 |
| EP1034309B1 (de) | 2002-05-08 |
| US6214556B1 (en) | 2001-04-10 |
| ES2173669T3 (es) | 2002-10-16 |
| PT1034309E (pt) | 2002-10-31 |
| IS1921B (is) | 2004-03-15 |
| HUP0100424A2 (hu) | 2001-06-28 |
| DE19754482A1 (de) | 1999-07-01 |
| RU2223324C2 (ru) | 2004-02-10 |
| EP1034309A2 (de) | 2000-09-13 |
| JP2001525181A (ja) | 2001-12-11 |
| CA2310384C (en) | 2007-05-22 |
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