ES2570828T3 - Método de análisis de metilación - Google Patents

Método de análisis de metilación

Info

Publication number
ES2570828T3
ES2570828T3 ES12164321T ES12164321T ES2570828T3 ES 2570828 T3 ES2570828 T3 ES 2570828T3 ES 12164321 T ES12164321 T ES 12164321T ES 12164321 T ES12164321 T ES 12164321T ES 2570828 T3 ES2570828 T3 ES 2570828T3
Authority
ES
Spain
Prior art keywords
terminal
oligonucleotide
nucleotides
seq
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES12164321T
Other languages
English (en)
Inventor
Theo Devos
Cathy Lofton-Day
Andrew Sledziewski
Fabian Model
Michael Krouse
Mathias Schuster
Jürgen Distler
Reimo Tetzner
Jesse Ho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
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Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Application granted granted Critical
Publication of ES2570828T3 publication Critical patent/ES2570828T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Un método para análisis de metilación, comprende a) tratar ADN genómico con uno o más reactivos para convertir bases de citosina no metiladas a sulfonato de uracilo o a otra base que tenga un comportamiento de unión diferente de citosina, mientras que la citosina metilada permanece sin cambio; b) amplificar el ADN tratado por medio de i) un oligonucleótido que comprende o que consiste de una secuencia definida por la SEQ ID NO: 5 o una variante de la misma con supresión de 5'-terminal y/o 3'-terminal de 1, 2, 3, 4 o 5 nucleótidos; y ii) un oligonucleótido que comprende o que consiste de una secuencia que se define por la SEQ ID NO: 44 o una variante de la misma con supresión de 5'-terminal y/o 3'-terminal de 1, 2, 3, 4 o 5 nucleótidos; en donde dichos oligonucleótidos son adecuados para uso como cebadores; c) deducir la presencia o ausencia de metilación de los dinucleótidos CpG amplificados en la etapa b) a partir de los resultados de la etapa b).
ES12164321T 2007-06-08 2008-06-06 Método de análisis de metilación Active ES2570828T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP07109907 2007-06-08
EP07110409 2007-06-15
EP07113516 2007-07-31
EP07114659 2007-08-21
EP07114863 2007-08-23
EP08150552 2008-01-23
EP08151442 2008-02-14

Publications (1)

Publication Number Publication Date
ES2570828T3 true ES2570828T3 (es) 2016-05-20

Family

ID=40094240

Family Applications (2)

Application Number Title Priority Date Filing Date
ES16163841T Active ES2769841T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación
ES12164321T Active ES2570828T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES16163841T Active ES2769841T3 (es) 2007-06-08 2008-06-06 Método de análisis de metilación

Country Status (12)

Country Link
US (1) US8623599B2 (es)
EP (3) EP3101141B1 (es)
CY (1) CY1118259T1 (es)
DK (2) DK2479289T3 (es)
ES (2) ES2769841T3 (es)
HK (1) HK1172656A1 (es)
HR (2) HRP20160425T1 (es)
HU (1) HUE028727T2 (es)
LT (1) LT3101141T (es)
PL (1) PL2479289T3 (es)
SI (2) SI3101141T1 (es)
WO (1) WO2008149237A2 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
JP5738278B2 (ja) * 2009-05-01 2015-06-24 キアジェン ゲイサーズバーグ インコーポレイテッド 試料中のｒｎａスプライシング形態を検出するための非標的増幅法
JP5826752B2 (ja) * 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
JP5702789B2 (ja) 2009-09-23 2015-04-15 コーニンクレッカ フィリップス エヌ ヴェ 亜硫酸水素塩により媒介されるｄｎａの変換を監視する方法
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
ES2615728T3 (es) 2010-01-29 2017-06-08 Qiagen Gaithersburg, Inc. Métodos y composiciones para una purificación y un análisis múltiple específico de secuencia de ácidos nucleicos
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
US9725754B2 (en) * 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
EP2678442B1 (en) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
CA3111723A1 (en) 2012-01-30 2013-08-08 Exact Sciences Development Company, Llc Sulfonated small dna compositions and methods for sulfonating and desulfonating small dnas
WO2013138644A2 (en) * 2012-03-15 2013-09-19 New England Biolabs, Inc. Methods and compositions for discrimination between cytosine and modifications thereof, and for methylome analysis
EP2746403A1 (de) 2012-12-21 2014-06-25 Life Science Inkubator GmbH & Co. KG Verfahren zur Bestimmung des DNA-Methylierungsgrades
CA2901293A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Detection of bisulfite converted nucleotide sequences
DK4026917T5 (da) 2014-04-14 2024-07-15 Yissum Research And Development Company Of The Hebrew Univ Of Jerusalem Ltd Fremgangsmåde og kit til bestemmelse af cellers eller vævs død eller dna's vævs- eller celleoprindelse ved dna-methyleringsanalyse
JP6731942B2 (ja) * 2014-12-19 2020-07-29 エピゲノミクスアーゲー ＣｐＧメチル化の検出方法及び癌の診断方法
AU2016343937B2 (en) 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
CN108495937A (zh) * 2015-11-27 2018-09-04 新加坡科技研究局 核酸甲基化的确定
CN105506133A (zh) * 2016-01-15 2016-04-20 武汉艾米森生命科技有限公司 用于检测sept9基因甲基化的组合物、试剂盒及方法
KR102436270B1 (ko) 2016-05-05 2022-08-25 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 폐 종양의 검출
WO2018140781A1 (en) 2017-01-27 2018-08-02 Exact Sciences Development Company, Llc Detection of colon neoplasia by analysis of methylated dna
CN107164524A (zh) * 2017-06-27 2017-09-15 深圳市优圣康生物科技有限公司 用于基因甲基化检测的引物和探针、采样方法、试剂盒
US20200165671A1 (en) * 2017-07-13 2020-05-28 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Detecting tissue-specific dna
US11466323B2 (en) 2017-07-13 2022-10-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Dual-probe digital droplet PCR strategy for specific detection of tissue-specific circulating DNA molecules
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN108048570A (zh) * 2017-12-29 2018-05-18 韩林志 用于结直肠癌相关基因Septin9甲基化检测的引物对、试剂盒及方法
CN110283913A (zh) * 2019-07-21 2019-09-27 广州奥百阕谱生物科技有限公司 Rassf1a基因甲基化状态检测试剂盒及其应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3525072A1 (de) 1985-07-13 1987-01-22 Basf Ag Verfahren zur herstellung von ueber o,o'-methylenether verknuepften phenolharzen und deren verwendung
DE3924052A1 (de) 1989-07-21 1991-01-24 Bayer Ag N- (indol-6-yl)-heterocyclen
CA2062918A1 (en) 1991-04-01 1992-10-02 Richard A. Carpenter Method for operating a networked computer system to minimize data conversion overhead
EP0833319A3 (en) 1993-01-21 2002-04-03 Matsushita Electric Industrial Co., Ltd. Disc-shaped recording medium
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
AU7829398A (en) 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
DE19957827C2 (de) 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010281B4 (de) 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
EP1352089B1 (en) 2000-08-25 2006-12-06 Lovelace Respiratory Research Institute Nested methylation-specific polymerase chain reaction cancer detection method
JP2004516821A (ja) 2000-09-01 2004-06-10 エピゲノミクス アーゲー ゲノムＤＮＡ上の、配列コンテクスト５−ＣｐＧ−３内の特定シトシンのメチル化度の定量方法
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
DE10338308B4 (de) 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
DE602004021192D1 (de) 2003-10-09 2009-07-02 Epigenomics Ag Verbesserte bisulfitkonversion von dna
WO2007039101A1 (en) 2005-09-30 2007-04-12 Epigenomics Ag Method for the quantification of nucleic acids, in particular bisulfite treated dna
US8679745B2 (en) 2004-09-30 2014-03-25 Epigenomics Ag Method for providing DNA fragments derived from an archived sample
US7700282B2 (en) 2004-10-11 2010-04-20 Epigenomics Ag Method for the carry-over protection in DNA amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids
WO2006088978A1 (en) 2005-02-16 2006-08-24 Epigenomics, Inc. Method for determining the methylation pattern of a polynucleic acid
US8129107B2 (en) 2005-04-01 2012-03-06 Epigenomics Ag Bisulfite conversion of DNA
DK1871912T3 (da) * 2005-04-15 2012-05-14 Epigenomics Ag Fremgangsmåde til bestemmelse af DNA-methylering i blod- eller urinprøver
CN107663538B (zh) 2005-04-15 2022-03-18 Epi基因组股份公司 分析细胞增殖性病症的方法和核酸

Also Published As

Publication number Publication date
PL2479289T3 (pl) 2016-10-31
US20110009277A1 (en) 2011-01-13
SI3101141T1 (sl) 2020-03-31
CY1118259T1 (el) 2017-06-28
DK3101141T3 (da) 2020-02-03
EP2479289A2 (en) 2012-07-25
EP3101141A2 (en) 2016-12-07
LT3101141T (lt) 2020-03-10
EP2479289A3 (en) 2013-06-19
HUE028727T2 (en) 2017-01-30
WO2008149237A2 (en) 2008-12-11
EP3101141A3 (en) 2017-01-11
HRP20160425T1 (hr) 2016-08-12
HRP20200059T1 (hr) 2020-04-03
WO2008149237A3 (en) 2009-06-04
EP2152902A2 (en) 2010-02-17
EP3101141B1 (en) 2019-10-16
DK2479289T3 (en) 2016-05-02
ES2769841T3 (es) 2020-06-29
HK1172656A1 (zh) 2013-04-26
SI2479289T1 (sl) 2016-10-28
EP2479289B1 (en) 2016-04-06
US8623599B2 (en) 2014-01-07

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