CY1118259T1 - Μεθοδος αναλυσης μεθυλιωσης - Google Patents

Μεθοδος αναλυσης μεθυλιωσης

Info

Publication number
CY1118259T1
CY1118259T1 CY20161100391T CY161100391T CY1118259T1 CY 1118259 T1 CY1118259 T1 CY 1118259T1 CY 20161100391 T CY20161100391 T CY 20161100391T CY 161100391 T CY161100391 T CY 161100391T CY 1118259 T1 CY1118259 T1 CY 1118259T1
Authority
CY
Cyprus
Prior art keywords
methylisis
analysis method
compositions
aspects
methods
Prior art date
Application number
CY20161100391T
Other languages
English (en)
Inventor
Theo Devos
Cathy Lofton-Day
Andrew Sledziewski
Fabian Model
Michael Krouse
Mathias Schuster
Jürgen Distler
Reimo Tetzner
Jesse Ho
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Publication of CY1118259T1 publication Critical patent/CY1118259T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Πτυχές της εφεύρεσης σχετίζονται με συνθέσεις και μεθόδους για την παροχή DNA για ανάλυση μεθυλίωσης που είναι ιδιαιτέρως κατάλληλη για εφαρμογή σε εργαστήρια αναφοράς. Περεταίρω 5 πτυχές της ανάπτυξης σχετίζονται με συνθέσεις και μεθόδους για την υψηλής ειδικότητας και ευαισθησίας ανάλυση μεθυλίωσης του γονιδίου SEPT9, επίσης ιδιαιτέρως κατάλληλη για εφαρμογή σε εργαστήρια αναφοράς.
CY20161100391T 2007-06-08 2016-05-10 Μεθοδος αναλυσης μεθυλιωσης CY1118259T1 (el)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP07109907 2007-06-08
EP07110409 2007-06-15
EP07113516 2007-07-31
EP07114659 2007-08-21
EP07114863 2007-08-23
EP08150552 2008-01-23
EP08151442 2008-02-14
EP08789067A EP2152902A2 (en) 2007-06-08 2008-06-06 Method for methylation analysis
EP12164321.7A EP2479289B1 (en) 2007-06-08 2008-06-06 Method for methylation analysis

Publications (1)

Publication Number Publication Date
CY1118259T1 true CY1118259T1 (el) 2017-06-28

Family

ID=40094240

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20161100391T CY1118259T1 (el) 2007-06-08 2016-05-10 Μεθοδος αναλυσης μεθυλιωσης

Country Status (12)

Country Link
US (1) US8623599B2 (el)
EP (3) EP2152902A2 (el)
CY (1) CY1118259T1 (el)
DK (2) DK3101141T3 (el)
ES (2) ES2769841T3 (el)
HK (1) HK1172656A1 (el)
HR (2) HRP20160425T1 (el)
HU (1) HUE028727T2 (el)
LT (1) LT3101141T (el)
PL (1) PL2479289T3 (el)
SI (2) SI3101141T1 (el)
WO (1) WO2008149237A2 (el)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
JP5738278B2 (ja) 2009-05-01 2015-06-24 キアジェン ゲイサーズバーグ インコーポレイテッド 試料中のｒｎａスプライシング形態を検出するための非標的増幅法
AU2010291990B2 (en) * 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US8889353B2 (en) 2009-09-23 2014-11-18 Koninklijke Philips N.V. Method for monitoring the bisulfite-mediated conversion of DNA
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
AU2011210734B2 (en) 2010-01-29 2017-02-09 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
WO2011146629A2 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
AU2011258501B2 (en) 2010-05-25 2016-07-07 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US9725754B2 (en) * 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
US9315853B2 (en) * 2012-01-30 2016-04-19 Exact Sciences Corporation Modification of DNA on magnetic beads
US9121061B2 (en) * 2012-03-15 2015-09-01 New England Biolabs, Inc. Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
EP2746403A1 (de) 2012-12-21 2014-06-25 Life Science Inkubator GmbH & Co. KG Verfahren zur Bestimmung des DNA-Methylierungsgrades
CN105026580A (zh) * 2013-03-15 2015-11-04 雅培分子公司 重亚硫酸盐转化的核苷酸序列的检测
LT4026917T (lt) 2014-04-14 2024-03-12 Yissum Research And Development Company Of The Hebrew University Of Jerusalem Ltd. Ląstelių ar audinio žūties nustatymo arba iš audinio ar ląstelės gautos dnr kilmės nustatymo dnr metilinimo analizės būdu metodas ir rinkinys
JP6731942B2 (ja) * 2014-12-19 2020-07-29 エピゲノミクスアーゲー ＣｐＧメチル化の検出方法及び癌の診断方法
CN108350485A (zh) * 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
US11021743B2 (en) * 2015-11-27 2021-06-01 Agency For Science, Technology And Research Determination of nucleic acid methylation
CN105506133A (zh) * 2016-01-15 2016-04-20 武汉艾米森生命科技有限公司 用于检测sept9基因甲基化的组合物、试剂盒及方法
US10385406B2 (en) 2016-05-05 2019-08-20 Exact Sciences Development Company, Llc Detection of lung neoplasia by analysis of methylated DNA
CN110475875B (zh) 2017-01-27 2024-06-25 精密科学公司 通过分析甲基化dna检测结肠瘤形成
CN107164524A (zh) * 2017-06-27 2017-09-15 深圳市优圣康生物科技有限公司 用于基因甲基化检测的引物和探针、采样方法、试剂盒
EP3652342A1 (en) * 2017-07-13 2020-05-20 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Detecting tissue-specific dna
US11466323B2 (en) 2017-07-13 2022-10-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Dual-probe digital droplet PCR strategy for specific detection of tissue-specific circulating DNA molecules
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN108048570A (zh) * 2017-12-29 2018-05-18 韩林志 用于结直肠癌相关基因Septin9甲基化检测的引物对、试剂盒及方法
CN110283913A (zh) * 2019-07-21 2019-09-27 广州奥百阕谱生物科技有限公司 Rassf1a基因甲基化状态检测试剂盒及其应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3525072A1 (de) 1985-07-13 1987-01-22 Basf Ag Verfahren zur herstellung von ueber o,o'-methylenether verknuepften phenolharzen und deren verwendung
DE3924052A1 (de) 1989-07-21 1991-01-24 Bayer Ag N- (indol-6-yl)-heterocyclen
CA2062918A1 (en) 1991-04-01 1992-10-02 Richard A. Carpenter Method for operating a networked computer system to minimize data conversion overhead
SG82519A1 (en) 1993-01-21 2001-08-21 Matsushita Electric Ind Co Ltd Information recording medium and method of fabricating the same
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
WO1998056952A1 (en) 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
DE19957827C2 (de) 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010281B4 (de) 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
ATE347619T1 (de) 2000-08-25 2006-12-15 Lovelace Respiratory Res Inst VERFAHREN ZUR KREBSDETEKTION MITTELS VERSCHACHTELTER (ßNESTEDß) METHYLIERUNGSSPEZIFISCHER PCR
KR100870486B1 (ko) 2000-09-01 2008-12-11 에피제노믹스 아게 게놈 디엔에이 시료의 서열 콘택스트 5'-씨피쥐-3'에서 특정 시토신의 메틸화 정도 측정방법
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
DE10338308B4 (de) 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
AU2004282342B2 (en) 2003-10-09 2009-05-14 Epigenomics Ag Improved bisulfite conversion of DNA
WO2007039101A1 (en) 2005-09-30 2007-04-12 Epigenomics Ag Method for the quantification of nucleic acids, in particular bisulfite treated dna
EP3061833B1 (en) 2004-09-30 2018-03-14 Epigenomics AG Method for providing dna fragments derived from an archived sample
JP4727670B2 (ja) 2004-10-11 2011-07-20 エピゲノミクス アーゲー 核酸の改変された前処理によって達成されるメチル化解析を標的とするｄｎａ増幅系におけるキャリーオーバー保護のための方法
WO2006088978A1 (en) 2005-02-16 2006-08-24 Epigenomics, Inc. Method for determining the methylation pattern of a polynucleic acid
EP2385136A1 (en) 2005-04-01 2011-11-09 Epigenomics AG Improved bisulfite conversion of DNA
SI1871912T1 (sl) * 2005-04-15 2012-06-29 Epigenomics Ag Postopek za določitev DNA metilacije v vzorcih krvi ali urina
CN107663538B (zh) * 2005-04-15 2022-03-18 Epi基因组股份公司 分析细胞增殖性病症的方法和核酸

Also Published As

Publication number Publication date
EP3101141B1 (en) 2019-10-16
LT3101141T (lt) 2020-03-10
ES2769841T3 (es) 2020-06-29
HUE028727T2 (en) 2017-01-30
EP2479289A2 (en) 2012-07-25
PL2479289T3 (pl) 2016-10-31
EP3101141A3 (en) 2017-01-11
HRP20160425T1 (hr) 2016-08-12
WO2008149237A3 (en) 2009-06-04
US20110009277A1 (en) 2011-01-13
SI3101141T1 (sl) 2020-03-31
ES2570828T3 (es) 2016-05-20
HRP20200059T1 (hr) 2020-04-03
DK2479289T3 (en) 2016-05-02
DK3101141T3 (da) 2020-02-03
WO2008149237A2 (en) 2008-12-11
US8623599B2 (en) 2014-01-07
HK1172656A1 (zh) 2013-04-26
EP2479289A3 (en) 2013-06-19
SI2479289T1 (sl) 2016-10-28
EP3101141A2 (en) 2016-12-07
EP2479289B1 (en) 2016-04-06
EP2152902A2 (en) 2010-02-17

Similar Documents

Publication Publication Date Title
CY1118259T1 (el) Μεθοδος αναλυσης μεθυλιωσης
CY1124522T1 (el) Ενωσεις και μεθοδοι για ρυθμιση της εκφρασης πρωτεϊνης κινασης μυοτονικης δυστροφιας (dmpk)
CY1121101T1 (el) Μεθοδοι και στερεα υποστρωματα για παρασκευη δειγματος χρησιμοποιωντας τρανσποσωματα
CY1123220T1 (el) Πρωτεϊνες συνδεσης anti-lag-3
CY1119563T1 (el) Βελτιστοποιηση αντισωματων που δεσμευουν το γονιδιο-3 ενεργοποιησης λεμφοκυτταρων (lag-3), και χρησεις εξ' αυτων
CY1124802T1 (el) Δεσμευτικοι παραγοντες σκληροστινης
CY1122091T1 (el) Μορια δεσμευσης-cd37 και ανοσοσυζευγματα εξ αυτων
CY1122101T1 (el) Τροποποιημενα πολυπεπτιδια ρελαξινης και χρησεις αυτων
CY1113713T1 (el) Ανοσοκατασταλτικα πολυπεπτιδια και νουκλεϊκα οξεα
CY1115188T1 (el) Πολυκυκλικες ετεροαρυλ-υποκατεστημενες τριαζολες χρησιμες ως αναστολεις του axl
CY1118760T1 (el) Τροποποιημενα αντισωματα εναντι il-23
CY1112297T1 (el) Ετεροκυκλικοι αναστολεις της μεκ και μεθοδοι χρησης αυτων
EA202090739A1 (ru) Белки, связывающие антиген созревания в-клеток
EP1994142A4 (en) METHODS AND COMPOSITIONS FOR RAPID ISOLATION OF SMALL RNA MOLECULES
CY1113089T1 (el) Ανθρωπινα μονοκλωνικα αντισωματα σε ειδικο προστατικο μεμβρανικο αντιγονο (psma)
CY1113199T1 (el) Σταθεροποιημενες συνθεσεις γλυκοσερεμπροσιδασης
BR112017017025A2 (pt) métodos e composições para analizar componentes celulares
CR20110245A (es) Mutantes fgf21 y uso de los mismos.
CL2012003196A1 (es) Anticuerpo aislado o fragmento del mismo que se une específicamente a interleuquina-4 (il-4); molécula de ácido nucleico aislado que lo codifica y vector que la comprende; célula que comprende dicho vector; composición farmacéutica que comprende dicho anticuerpo; y su uso parar tratar una enfermedad alérgica, cáncer y asma.
CY1112553T1 (el) Ανοσοπροσδιορισμοι υψηλης ευαισθησιας και κιτια για τον προσδιορισμο πεπτιδιων και πρωτεϊνων βιολογικου ενδιαφεροντος
CY1108092T1 (el) Ενωσεις και μεθοδοι για την αναστολη της μιτωτικης προοδου με την αναστολη της κινασης aurora
CY1114904T1 (el) Prlr-ειδικο αντισωμα και χρησεις αυτου
DK1842226T3 (da) Homogen analytpåvisning
CY1113757T1 (el) Παραγωγα πιπεριδινης και πιπεραζινης για τη θεραπεια ογκων
CY1115735T1 (el) Τα διαζοδικυκλα μορια μιμητες smac και οι χρησεις αυτων