ATE347619T1 - VERFAHREN ZUR KREBSDETEKTION MITTELS VERSCHACHTELTER (ßNESTEDß) METHYLIERUNGSSPEZIFISCHER PCR - Google Patents
VERFAHREN ZUR KREBSDETEKTION MITTELS VERSCHACHTELTER (ßNESTEDß) METHYLIERUNGSSPEZIFISCHER PCRInfo
- Publication number
- ATE347619T1 ATE347619T1 AT01968105T AT01968105T ATE347619T1 AT E347619 T1 ATE347619 T1 AT E347619T1 AT 01968105 T AT01968105 T AT 01968105T AT 01968105 T AT01968105 T AT 01968105T AT E347619 T1 ATE347619 T1 AT E347619T1
- Authority
- AT
- Austria
- Prior art keywords
- gene
- nested
- detecting
- cancer
- methylation
- Prior art date
Links
- 206010028980 Neoplasm Diseases 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 5
- 201000011510 cancer Diseases 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 2
- 238000007855 methylation-specific PCR Methods 0.000 title 1
- 230000001594 aberrant effect Effects 0.000 abstract 2
- 230000011987 methylation Effects 0.000 abstract 2
- 238000007069 methylation reaction Methods 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 2
- 101150084750 1 gene Proteins 0.000 abstract 1
- 101000796998 Bacillus subtilis (strain 168) Methylated-DNA-protein-cysteine methyltransferase, inducible Proteins 0.000 abstract 1
- 108010031042 Death-Associated Protein Kinases Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 206010036790 Productive cough Diseases 0.000 abstract 1
- 239000013060 biological fluid Substances 0.000 abstract 1
- 210000004072 lung Anatomy 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000003147 molecular marker Substances 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 108700042657 p16 Genes Proteins 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 210000003802 sputum Anatomy 0.000 abstract 1
- 208000024794 sputum Diseases 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22805700P | 2000-08-25 | 2000-08-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE347619T1 true ATE347619T1 (de) | 2006-12-15 |
Family
ID=22855593
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01968105T ATE347619T1 (de) | 2000-08-25 | 2001-08-24 | VERFAHREN ZUR KREBSDETEKTION MITTELS VERSCHACHTELTER (ßNESTEDß) METHYLIERUNGSSPEZIFISCHER PCR |
Country Status (7)
Country | Link |
---|---|
US (1) | US7214485B2 (de) |
EP (1) | EP1352089B1 (de) |
AT (1) | ATE347619T1 (de) |
AU (1) | AU2001288379A1 (de) |
CA (1) | CA2421102A1 (de) |
DE (1) | DE60125065T2 (de) |
WO (1) | WO2002018649A1 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6818404B2 (en) * | 1997-10-23 | 2004-11-16 | Exact Sciences Corporation | Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
US20040053304A1 (en) * | 2002-06-05 | 2004-03-18 | Case Western Reserve University | Methods and compositions for detecting cancers |
WO2004050915A1 (en) * | 2002-12-02 | 2004-06-17 | Solexa Limited | Determination of methylation of nucleic acid sequences |
AU2003221217A1 (en) * | 2003-03-13 | 2004-09-30 | Beijing Institute For Cancer Research | The method of in vitro detecting aberrant dysplasia, and the artificial nucleotides being used |
AU2004284456B2 (en) | 2003-10-21 | 2012-04-19 | Orion Genomics Llc | Methods for quantitative determination of methylation density in a DNA locus |
EP1692264A2 (de) * | 2003-10-28 | 2006-08-23 | The Johns Hopkins University | Quantitative methylierungsspezifische multiplex-pcr |
US20050196792A1 (en) * | 2004-02-13 | 2005-09-08 | Affymetrix, Inc. | Analysis of methylation status using nucleic acid arrays |
DE602005019174D1 (de) * | 2004-06-17 | 2010-03-18 | Epigenomics Ag | Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen |
ES2521115T3 (es) | 2004-09-30 | 2014-11-12 | Epigenomics Ag | Método para proveer fragmentos de ADN derivados de una muestra archivada |
WO2006113770A1 (en) | 2005-04-15 | 2006-10-26 | Epigenomics Ag | A method for providing dna fragments derived from a remote sample |
WO2007008693A2 (en) * | 2005-07-09 | 2007-01-18 | Lovelace Respiratory Research Institute | Gene methylation as a biomarker in sputum |
US7901882B2 (en) * | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
US20080003609A1 (en) * | 2006-05-10 | 2008-01-03 | The Cleveland Clinic Foundation | Method of detecting bladder urothelial carcinoma |
CN101153336B (zh) | 2006-09-27 | 2011-09-07 | 香港中文大学 | 检测dna甲基化程度的方法和试剂盒 |
US7599898B2 (en) * | 2006-10-17 | 2009-10-06 | International Business Machines Corporation | Method and apparatus for improved regression modeling |
CA2670082A1 (en) * | 2006-11-20 | 2008-05-29 | The Johns Hopkins University | Dna methylation markers and methods of use |
WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
DK3101141T3 (da) | 2007-06-08 | 2020-02-03 | Epigenomics Ag | Fremgangsmåde til methyleringsanalyse |
WO2009047756A2 (en) * | 2007-10-11 | 2009-04-16 | Bioview Ltd. | Methods and kits for diagnosing lung cancer |
WO2010048337A2 (en) | 2008-10-22 | 2010-04-29 | Illumina, Inc. | Preservation of information related to genomic dna methylation |
US8394584B2 (en) | 2008-12-19 | 2013-03-12 | The Board Of Trustees Of The University Of Illinois | Detecting and sorting methylated DNA using a synthetic nanopore |
EP2218793A1 (de) * | 2009-02-13 | 2010-08-18 | Alphagenics International SA | Erkennung von MGMT-Methylierung in Tumoren |
US8748091B2 (en) * | 2009-12-18 | 2014-06-10 | The Board Of Trustees Of The University Of Illinois | Characterizing stretched polynucleotides in a synthetic nanopassage |
JP5765586B2 (ja) | 2010-04-21 | 2015-08-19 | 国立大学法人 鹿児島大学 | 新規なdnaメチル化解析方法 |
CN104250663B (zh) * | 2013-06-27 | 2017-09-15 | 北京大学 | 甲基化CpG岛的高通量测序检测方法 |
CN116144755A (zh) * | 2022-12-30 | 2023-05-23 | 中国辐射防护研究院 | LncRNA作为氡暴露早期损伤诊断分子标志物的应用及筛选方法 |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5965722A (en) * | 1991-05-21 | 1999-10-12 | Isis Pharmaceuticals, Inc. | Antisense inhibition of ras gene with chimeric and alternating oligonucleotides |
DE69213112T2 (de) | 1991-06-20 | 1997-04-03 | Hoffmann La Roche | Verbesserte Methoden zur Nukleinsäure-Amplifizierung |
JPH0690756A (ja) | 1992-09-10 | 1994-04-05 | Fuso Yakuhin Kogyo Kk | 2段サーモサイクルpcr法 |
US5556773A (en) * | 1993-08-06 | 1996-09-17 | Yourno; Joseph | Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes |
US5565331A (en) * | 1993-11-12 | 1996-10-15 | The Regents Of The University Of California | Nucleic acids encoding neural axon outgrowth modulators |
US5986076A (en) * | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
US5843649A (en) * | 1995-03-01 | 1998-12-01 | University Of Colorado | Method of identifying clonal cell samples using heteroduplex generators |
US5693467A (en) * | 1995-05-19 | 1997-12-02 | The American Type Culture Collection | Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers |
US5871917A (en) * | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
US6017704A (en) * | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
WO1998056952A1 (en) * | 1997-06-09 | 1998-12-17 | University Of Southern California | A cancer diagnostic method based upon dna methylation differences |
US6818404B2 (en) * | 1997-10-23 | 2004-11-16 | Exact Sciences Corporation | Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples |
US6033858A (en) * | 1998-03-30 | 2000-03-07 | Bastian; Frank O. | Detection of transmissible spongiform encephalopathies |
GB9811598D0 (en) | 1998-05-30 | 1998-07-29 | Imp College Innovations Ltd | Diagnosis and treatment of cancer |
AU5128999A (en) * | 1998-07-24 | 2000-02-14 | Yeda Research And Development Co. Ltd. | Prevention of metastasis with 5-aza-2'-deoxycytidine |
US6143504A (en) * | 1998-10-27 | 2000-11-07 | Arch Development Corporation | Methods and compositions for the diagnosis of fragile X syndrome |
US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
AU6000600A (en) | 1999-07-15 | 2001-02-05 | University Of Bristol, The | Diagnostic method |
US6709818B1 (en) * | 1999-10-13 | 2004-03-23 | The Johns Hopkins University School Of Medicine | Methods of diagnosing and treating hepatic cell proliferative disorders |
-
2001
- 2001-08-24 EP EP01968105A patent/EP1352089B1/de not_active Revoked
- 2001-08-24 AU AU2001288379A patent/AU2001288379A1/en not_active Abandoned
- 2001-08-24 CA CA002421102A patent/CA2421102A1/en not_active Abandoned
- 2001-08-24 WO PCT/US2001/026452 patent/WO2002018649A1/en active IP Right Grant
- 2001-08-24 DE DE60125065T patent/DE60125065T2/de not_active Revoked
- 2001-08-24 AT AT01968105T patent/ATE347619T1/de not_active IP Right Cessation
- 2001-08-24 US US10/344,815 patent/US7214485B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP1352089A1 (de) | 2003-10-15 |
US20040038245A1 (en) | 2004-02-26 |
EP1352089A4 (de) | 2004-10-20 |
DE60125065T2 (de) | 2007-06-28 |
WO2002018649A1 (en) | 2002-03-07 |
US7214485B2 (en) | 2007-05-08 |
AU2001288379A1 (en) | 2002-03-13 |
CA2421102A1 (en) | 2002-03-07 |
EP1352089B1 (de) | 2006-12-06 |
DE60125065D1 (de) | 2007-01-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |