DE602005019174D1 - Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen - Google Patents

Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen

Info

Publication number
DE602005019174D1
DE602005019174D1 DE602005019174T DE602005019174T DE602005019174D1 DE 602005019174 D1 DE602005019174 D1 DE 602005019174D1 DE 602005019174 T DE602005019174 T DE 602005019174T DE 602005019174 T DE602005019174 T DE 602005019174T DE 602005019174 D1 DE602005019174 D1 DE 602005019174D1
Authority
DE
Germany
Prior art keywords
nucleic acid
contaminating
amplificate
methods
carry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005019174T
Other languages
English (en)
Inventor
Reimo Tetzner
Kurt Berlin
Juergen Distler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of DE602005019174D1 publication Critical patent/DE602005019174D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE602005019174T 2004-06-17 2005-06-17 Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen Active DE602005019174D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58064404P 2004-06-17 2004-06-17
US58052904P 2004-06-17 2004-06-17
US58063804P 2004-06-17 2004-06-17
PCT/US2005/021525 WO2006009870A2 (en) 2004-06-17 2005-06-17 Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions

Publications (1)

Publication Number Publication Date
DE602005019174D1 true DE602005019174D1 (de) 2010-03-18

Family

ID=34972771

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005019174T Active DE602005019174D1 (de) 2004-06-17 2005-06-17 Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen

Country Status (5)

Country Link
US (1) US20080311627A1 (de)
EP (1) EP1781813B1 (de)
AT (1) ATE456672T1 (de)
DE (1) DE602005019174D1 (de)
WO (1) WO2006009870A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
WO2007141034A1 (en) * 2006-06-08 2007-12-13 Epigenomics Ag Carry-over protection in enzyme-based dna amplification systems targeting methylation analysis
EP2480685B1 (de) 2009-09-23 2013-08-28 Koninklijke Philips Electronics N.V. Verfahren zur überwachung der bisulfitvermittelten umwandlung von dna
ES2449665B2 (es) * 2012-09-19 2014-07-17 Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua).
WO2014081511A1 (en) * 2012-11-21 2014-05-30 Courtagen Life Sciences Inc. Method for preventing carry-over contamination in nucleic acid amplification reactions
US12054724B2 (en) 2018-04-10 2024-08-06 President And Fellows Of Harvard College AAV vectors encoding clarin-1 or GJB2 and uses thereof
CN112301102B (zh) * 2019-07-31 2024-06-25 华南师范大学 一种高特异性的环介导等温扩增方法及其应用
EP4107264A4 (de) * 2020-02-21 2024-06-05 Mission Bio, Inc. Verbesserter nachweis von zielnukleinsäuren durch entfernung von dna-rna-kreuzkontamination
WO2022056444A1 (en) * 2020-09-14 2022-03-17 President And Fellows Of Harvard College Recombinant adeno associated virus (raav) encoding gjb2 and uses thereof
CN114317799B (zh) * 2021-12-21 2023-10-13 中国中医科学院中药研究所 一种鉴定当归及常见当归混伪品的特异性引物对及其应用
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
JP3392863B2 (ja) * 1990-07-24 2003-03-31 エフ．ホフマン ― ラ ロシュ アーゲー 修飾核酸塩基を使用するインビトロ核酸増幅における非特異的増幅の低減
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
GB9716664D0 (en) * 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US6605432B1 (en) * 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
EP1352089B1 (de) * 2000-08-25 2006-12-06 Lovelace Respiratory Research Institute Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr
EP1438428A4 (de) * 2001-09-14 2005-11-16 Univ Queensland Nachweis der methylierung von dna
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
DE102004002257B4 (de) * 2004-01-09 2006-11-09 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA mit Hilfe von DNA-Reparaturenzymen

Also Published As

Publication number Publication date
EP1781813A2 (de) 2007-05-09
ATE456672T1 (de) 2010-02-15
WO2006009870A3 (en) 2006-06-22
WO2006009870A2 (en) 2006-01-26
EP1781813B1 (de) 2010-01-27
US20080311627A1 (en) 2008-12-18

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