ATE456672T1 - Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen - Google Patents

Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen

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Publication number
ATE456672T1
ATE456672T1 AT05762161T AT05762161T ATE456672T1 AT E456672 T1 ATE456672 T1 AT E456672T1 AT 05762161 T AT05762161 T AT 05762161T AT 05762161 T AT05762161 T AT 05762161T AT E456672 T1 ATE456672 T1 AT E456672T1
Authority
AT
Austria
Prior art keywords
nucleic acid
contaminating
methods
amplificate
carry
Prior art date
Application number
AT05762161T
Other languages
English (en)
Inventor
Reimo Tetzner
Kurt Berlin
Juergen Distler
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Application granted granted Critical
Publication of ATE456672T1 publication Critical patent/ATE456672T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT05762161T 2004-06-17 2005-06-17 Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen ATE456672T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58063804P 2004-06-17 2004-06-17
US58052904P 2004-06-17 2004-06-17
US58064404P 2004-06-17 2004-06-17
PCT/US2005/021525 WO2006009870A2 (en) 2004-06-17 2005-06-17 Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions

Publications (1)

Publication Number Publication Date
ATE456672T1 true ATE456672T1 (de) 2010-02-15

Family

ID=34972771

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05762161T ATE456672T1 (de) 2004-06-17 2005-06-17 Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen

Country Status (5)

Country Link
US (1) US20080311627A1 (de)
EP (1) EP1781813B1 (de)
AT (1) ATE456672T1 (de)
DE (1) DE602005019174D1 (de)
WO (1) WO2006009870A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
EP2027287A1 (de) * 2006-06-08 2009-02-25 Epigenomics AG Carry-over-schutz bei dna-amplifikationssystemen auf enzymbasis mit methylierungsanalyse als ziel
EP2480685B1 (de) 2009-09-23 2013-08-28 Koninklijke Philips Electronics N.V. Verfahren zur überwachung der bisulfitvermittelten umwandlung von dna
ES2449665B2 (es) * 2012-09-19 2014-07-17 Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua).
CA3140836A1 (en) * 2012-11-21 2014-05-30 Courtagen Life Sciences Inc. A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample
US20230101896A1 (en) * 2020-02-21 2023-03-30 Mission Bio, Inc. Enhanced Detection of Target Nucleic Acids by Removal of DNA-RNA Cross Contamination
TW202227476A (zh) * 2020-09-14 2022-07-16 哈佛大學校長及研究員協會 編碼GJB2之重組腺相關病毒(rAAV)及其用途
CN114317799B (zh) * 2021-12-21 2023-10-13 中国中医科学院中药研究所 一种鉴定当归及常见当归混伪品的特异性引物对及其应用
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
CA2087724C (en) * 1990-07-24 2003-09-16 John J. Sninsky Reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
GB9716664D0 (en) * 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US6605432B1 (en) * 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
WO2002018649A1 (en) * 2000-08-25 2002-03-07 Lovelace Respiratory Research Institute Nested methylation-specific polymerase chain reaction cancer detection method
WO2003025215A1 (en) * 2001-09-14 2003-03-27 The University Of Queensland Detection of dna methylation
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
DE102004002257B4 (de) * 2004-01-09 2006-11-09 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA mit Hilfe von DNA-Reparaturenzymen

Also Published As

Publication number Publication date
WO2006009870A3 (en) 2006-06-22
WO2006009870A2 (en) 2006-01-26
US20080311627A1 (en) 2008-12-18
DE602005019174D1 (de) 2010-03-18
EP1781813B1 (de) 2010-01-27
EP1781813A2 (de) 2007-05-09

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