DE60125065D1 - Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr - Google Patents

Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr

Info

Publication number
DE60125065D1
DE60125065D1 DE60125065T DE60125065T DE60125065D1 DE 60125065 D1 DE60125065 D1 DE 60125065D1 DE 60125065 T DE60125065 T DE 60125065T DE 60125065 T DE60125065 T DE 60125065T DE 60125065 D1 DE60125065 D1 DE 60125065D1
Authority
DE
Germany
Prior art keywords
gene
detecting
cancer
humans
methody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE60125065T
Other languages
English (en)
Other versions
DE60125065T2 (de
Inventor
A Belinsky
A Palmisano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lovelace Respiratory Research Institute
LOVELACE RESPIRATORY RES INST
Original Assignee
Lovelace Respiratory Research Institute
LOVELACE RESPIRATORY RES INST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22855593&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60125065(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lovelace Respiratory Research Institute, LOVELACE RESPIRATORY RES INST filed Critical Lovelace Respiratory Research Institute
Application granted granted Critical
Publication of DE60125065D1 publication Critical patent/DE60125065D1/de
Publication of DE60125065T2 publication Critical patent/DE60125065T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60125065T 2000-08-25 2001-08-24 Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr Revoked DE60125065T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22805700P 2000-08-25 2000-08-25
US228057P 2000-08-25
PCT/US2001/026452 WO2002018649A1 (en) 2000-08-25 2001-08-24 Nested methylation-specific polymerase chain reaction cancer detection method

Publications (2)

Publication Number Publication Date
DE60125065D1 true DE60125065D1 (de) 2007-01-18
DE60125065T2 DE60125065T2 (de) 2007-06-28

Family

ID=22855593

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60125065T Revoked DE60125065T2 (de) 2000-08-25 2001-08-24 Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr

Country Status (7)

Country Link
US (1) US7214485B2 (de)
EP (1) EP1352089B1 (de)
AT (1) ATE347619T1 (de)
AU (1) AU2001288379A1 (de)
CA (1) CA2421102A1 (de)
DE (1) DE60125065T2 (de)
WO (1) WO2002018649A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2488382A1 (en) * 2002-06-05 2003-12-18 Case Western Reserve University Methods and compositions for detecting cancers
AU2003290223A1 (en) * 2002-12-02 2004-06-23 Solexa Limited Determination of methylation of nucleic acid sequences
DE60333680D1 (de) * 2003-03-13 2010-09-16 Beijing Inst Cancer Res VERFAHREN ZUR IN-VITRO-ERKENNUNG VON ANORMALER Magen-DYSPLASIE
ES2382780T3 (es) 2003-10-21 2012-06-13 Orion Genomics, Llc Procedimientos para la determinación cuantitativa de la densidad de metilación en un locus de ADN
US8062849B2 (en) * 2003-10-28 2011-11-22 The Johns Hopkins University Quantitative multiplex methylation-specific PCR
US20050196792A1 (en) * 2004-02-13 2005-09-08 Affymetrix, Inc. Analysis of methylation status using nucleic acid arrays
US20080311627A1 (en) * 2004-06-17 2008-12-18 Epigenomics Ag Compositions and Methods for Preventing Carry-Over Contamination in Nucleic Acid Amplification Reactions
ES2787454T3 (es) 2004-09-30 2020-10-16 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
ATE547537T1 (de) 2005-04-15 2012-03-15 Epigenomics Ag Verfahren zur bestimmung der dna-methylierung in blut- oder urinproben
WO2007008693A2 (en) * 2005-07-09 2007-01-18 Lovelace Respiratory Research Institute Gene methylation as a biomarker in sputum
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20080003609A1 (en) * 2006-05-10 2008-01-03 The Cleveland Clinic Foundation Method of detecting bladder urothelial carcinoma
TWI335354B (en) 2006-09-27 2011-01-01 Univ Hong Kong Chinese Methods for the detection of the degree of the methylation of a target dna and kits
US7599898B2 (en) * 2006-10-17 2009-10-06 International Business Machines Corporation Method and apparatus for improved regression modeling
EP2508623B1 (de) 2006-11-20 2015-08-12 The Johns Hopkins University DNA-Methylierungsmarker und Verwendungsverfahren
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
LT3101141T (lt) 2007-06-08 2020-03-10 Epigenomics Ag Metilinimo analizės būdas
WO2009047756A2 (en) * 2007-10-11 2009-04-16 Bioview Ltd. Methods and kits for diagnosing lung cancer
US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
WO2010080617A2 (en) 2008-12-19 2010-07-15 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated dna using a synthetic nanopore
EP2218793A1 (de) * 2009-02-13 2010-08-18 Alphagenics International SA Erkennung von MGMT-Methylierung in Tumoren
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
JP5765586B2 (ja) * 2010-04-21 2015-08-19 国立大学法人 鹿児島大学 新規なｄｎａメチル化解析方法
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
CN116144755A (zh) * 2022-12-30 2023-05-23 中国辐射防护研究院 LncRNA作为氡暴露早期损伤诊断分子标志物的应用及筛选方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5965722A (en) * 1991-05-21 1999-10-12 Isis Pharmaceuticals, Inc. Antisense inhibition of ras gene with chimeric and alternating oligonucleotides
DK0519338T3 (da) 1991-06-20 1996-10-28 Hoffmann La Roche Forbedrede fremgangsmåder til nukleinsyreamplifikation
JPH0690756A (ja) 1992-09-10 1994-04-05 Fuso Yakuhin Kogyo Kk ２段サーモサイクルｐｃｒ法
US5556773A (en) * 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5565331A (en) * 1993-11-12 1996-10-15 The Regents Of The University Of California Nucleic acids encoding neural axon outgrowth modulators
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5843649A (en) * 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US6033858A (en) * 1998-03-30 2000-03-07 Bastian; Frank O. Detection of transmissible spongiform encephalopathies
GB9811598D0 (en) 1998-05-30 1998-07-29 Imp College Innovations Ltd Diagnosis and treatment of cancer
WO2000005419A1 (en) * 1998-07-24 2000-02-03 Yeda Research And Development Company Ltd. Prevention of metastasis with 5-aza-2'-deoxycytidine
US6143504A (en) * 1998-10-27 2000-11-07 Arch Development Corporation Methods and compositions for the diagnosis of fragile X syndrome
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
WO2001006005A2 (en) * 1999-07-15 2001-01-25 The University Of Bristol Diagnostic method comprising wt1 sequences
US6709818B1 (en) * 1999-10-13 2004-03-23 The Johns Hopkins University School Of Medicine Methods of diagnosing and treating hepatic cell proliferative disorders

Also Published As

Publication number Publication date
ATE347619T1 (de) 2006-12-15
CA2421102A1 (en) 2002-03-07
US20040038245A1 (en) 2004-02-26
US7214485B2 (en) 2007-05-08
EP1352089A4 (de) 2004-10-20
AU2001288379A1 (en) 2002-03-13
WO2002018649A1 (en) 2002-03-07
EP1352089A1 (de) 2003-10-15
DE60125065T2 (de) 2007-06-28
EP1352089B1 (de) 2006-12-06

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8331 Complete revocation