IL135776A0 - Recombinational cloning using nucleic acids having recombination sites - Google Patents
Recombinational cloning using nucleic acids having recombination sitesInfo
- Publication number
- IL135776A0 IL135776A0 IL13577698A IL13577698A IL135776A0 IL 135776 A0 IL135776 A0 IL 135776A0 IL 13577698 A IL13577698 A IL 13577698A IL 13577698 A IL13577698 A IL 13577698A IL 135776 A0 IL135776 A0 IL 135776A0
- Authority
- IL
- Israel
- Prior art keywords
- nucleic acids
- recombination sites
- recombinational cloning
- dna molecules
- recombination
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Gas Separation By Absorption (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6593097P | 1997-10-24 | 1997-10-24 | |
US17738798A | 1998-10-23 | 1998-10-23 | |
PCT/US1998/022589 WO1999021977A1 (en) | 1997-10-24 | 1998-10-26 | Recombinational cloning using nucleic acids having recombination sites |
Publications (1)
Publication Number | Publication Date |
---|---|
IL135776A0 true IL135776A0 (en) | 2001-05-20 |
Family
ID=26746186
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL13577698A IL135776A0 (en) | 1997-10-24 | 1998-10-26 | Recombinational cloning using nucleic acids having recombination sites |
Country Status (12)
Country | Link |
---|---|
US (15) | US6277608B1 (xx) |
EP (1) | EP1025217B1 (xx) |
JP (2) | JP4303418B2 (xx) |
CN (1) | CN100342008C (xx) |
AT (1) | ATE341621T1 (xx) |
AU (1) | AU752704B2 (xx) |
CA (1) | CA2307016A1 (xx) |
DE (1) | DE69836092T2 (xx) |
HK (2) | HK1030019A1 (xx) |
IL (1) | IL135776A0 (xx) |
NZ (3) | NZ533288A (xx) |
WO (1) | WO1999021977A1 (xx) |
Families Citing this family (268)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
DE69623057T2 (de) | 1995-06-07 | 2003-03-27 | Invitrogen Corp., Carlsbad | Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte |
US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
US7235716B2 (en) | 1997-06-03 | 2007-06-26 | Chromatin, Inc. | Plant centromere compositions |
US7227057B2 (en) | 1997-06-03 | 2007-06-05 | Chromatin, Inc. | Plant centromere compositions |
US7193128B2 (en) | 1997-06-03 | 2007-03-20 | Chromatin, Inc. | Methods for generating or increasing revenues from crops |
US7119250B2 (en) | 1997-06-03 | 2006-10-10 | The University Of Chicago | Plant centromere compositions |
ATE341621T1 (de) | 1997-10-24 | 2006-10-15 | Invitrogen Corp | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
AU766257C (en) | 1998-05-07 | 2004-06-03 | Universite Libre De Bruxelles | Cytotoxin-based biological containment |
WO2000005355A1 (en) * | 1998-07-24 | 2000-02-03 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
CN1331700A (zh) | 1998-11-16 | 2002-01-16 | 基因纬生物技术公司 | 通过禽类中使用多核苷酸疫苗接种生成抗体的方法 |
NZ525134A (en) * | 1999-03-02 | 2004-09-24 | Invitrogen Corp | Compositions and methods for use in recombinational cloning of nucleic acids |
US7989202B1 (en) | 1999-03-18 | 2011-08-02 | The University Of Chicago | Plant centromere compositions |
US6709852B1 (en) * | 1999-06-22 | 2004-03-23 | Invitrogen Corporation | Rapid growing microorganisms for biotechnology applications |
DE19941186A1 (de) * | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
GB9921937D0 (en) * | 1999-09-17 | 1999-11-17 | Univ Leeds | Targeted gens removal |
NZ529476A (en) * | 1999-10-25 | 2005-03-24 | Invitrogen Corp | Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination |
CN1757724B (zh) * | 1999-12-10 | 2014-06-11 | 茵维特罗根公司 | 具有独特特异性的多个重组位点在重组克隆中的用途 |
US7244560B2 (en) | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6551828B1 (en) | 2000-06-28 | 2003-04-22 | Protemation, Inc. | Compositions and methods for generating expression vectors through site-specific recombination |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
JP4830079B2 (ja) * | 2000-11-29 | 2011-12-07 | 国立感染症研究所長 | プロテアーゼ活性の測定方法 |
CA2430947A1 (en) | 2000-12-08 | 2002-06-13 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US7214515B2 (en) * | 2001-01-05 | 2007-05-08 | The General Hospital Corporation | Viral delivery system for infectious transfer of large genomic DNA inserts |
CA2435956C (en) | 2001-02-23 | 2012-07-10 | Universite Libre De Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule |
JP2004531259A (ja) * | 2001-04-19 | 2004-10-14 | インヴィトロジェン コーポレーション | 核酸分子の組換えクローニングのための組成物および方法 |
CA2448505A1 (en) * | 2001-05-21 | 2002-11-28 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
CA2460617A1 (en) * | 2001-08-28 | 2003-03-13 | Japan Tobacco Inc. | Method of modifying genome in higher plant |
AU2002356891A1 (en) * | 2001-11-02 | 2003-05-19 | Intradigm Corporation | Method and system for inducible recombinational cloning in bacterial cells |
JP4210769B2 (ja) * | 2001-11-22 | 2009-01-21 | 独立行政法人産業技術総合研究所 | Gatewayエントリークローンの作製方法 |
US7338800B2 (en) * | 2002-01-16 | 2008-03-04 | Baylor College Of Medicine | In vivo gene transfer |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
US20040005710A1 (en) * | 2002-03-09 | 2004-01-08 | Ho-Sun Son | Method for producing recombinant viruses using site-specific recombination |
US20060130179A1 (en) | 2002-03-29 | 2006-06-15 | Suttie Janet L | Lambda integrase mediated recombination in plants |
AU2002257486A1 (en) * | 2002-04-25 | 2003-11-10 | Institute Of Zoology, Chinese Academy Of Sciences | Method for constructing a linear expression vector without cloning |
AU2003231244A1 (en) * | 2002-05-02 | 2003-11-17 | University Of North Carolina At Chapel Hill | In vitro mutagenesis, phenotyping, and gene mapping |
US20030235814A1 (en) * | 2002-06-19 | 2003-12-25 | Los Alamos National Laboratory | Compositions and methods for selecting open reading frames |
US20040132133A1 (en) * | 2002-07-08 | 2004-07-08 | Invitrogen Corporation | Methods and compositions for the production, identification and purification of fusion proteins |
AU2003253992A1 (en) * | 2002-07-18 | 2004-02-09 | Robert P. Bennett | Viral vectors containing recombination sites |
US9309518B2 (en) | 2002-09-03 | 2016-04-12 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
WO2004022745A2 (en) | 2002-09-03 | 2004-03-18 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
US20040115642A1 (en) * | 2002-12-12 | 2004-06-17 | Changlin Fu | Single-step methods for gene cloning |
US7078234B2 (en) | 2002-12-18 | 2006-07-18 | Monsanto Technology Llc | Maize embryo-specific promoter compositions and methods for use thereof |
WO2004065568A2 (en) * | 2003-01-23 | 2004-08-05 | Invitrogen Corporation | Rapid growing microorganisms for biotechnology applications |
EP1616949A4 (en) * | 2003-04-07 | 2006-06-21 | Jujo Paper Co Ltd | NEW VECTOR AND METHOD FOR CONSTRUCTING A PLANT TRANSFORMANT USING THE VECTOR |
EP1881074B1 (en) | 2003-05-05 | 2013-01-02 | Monsanto Technology, LLC | HRGP promoter constructs |
AU2004239760A1 (en) * | 2003-05-09 | 2004-11-25 | Health Research Inc. | Improved methods for protein interaction determination |
AU2003239211A1 (en) * | 2003-05-15 | 2004-12-13 | Invitrogen Corporation | Compositions and methods for site-specific recombination and expression |
EP1644538A4 (en) * | 2003-06-26 | 2006-11-08 | Invitrogen Corp | METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS |
US20050069929A1 (en) | 2003-08-08 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for seamless cloning of nucleic acid molecules |
EP1699928B9 (en) | 2003-10-02 | 2010-11-03 | Monsanto Technology, LLC | Stacking crop improvement traits in transgenic plants |
EP2361984A1 (en) | 2003-10-09 | 2011-08-31 | E. I. du Pont de Nemours and Company | Gene silencing by using modified micro-RNA molecules |
EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
US7935862B2 (en) | 2003-12-02 | 2011-05-03 | Syngenta Participations Ag | Targeted integration and stacking of DNA through homologous recombination |
US20050227309A1 (en) | 2004-01-21 | 2005-10-13 | Corry Schuyler B | Optically-detectable enzyme substrates and their method of use |
AR047598A1 (es) | 2004-02-10 | 2006-01-25 | Monsanto Technology Llc | Semilla de maiz transgenica con mayor contenido de aminoacidos |
US8729341B2 (en) * | 2004-02-23 | 2014-05-20 | University Of Chicago | Plants modified with mini-chromosomes |
US20090098611A1 (en) * | 2004-02-27 | 2009-04-16 | Wood David W | Self-cleaving affinity tags and methods of use |
US20050282265A1 (en) * | 2004-04-19 | 2005-12-22 | Laura Vozza-Brown | Electroporation apparatus and methods |
BRPI0511046A (pt) * | 2004-05-10 | 2007-11-27 | Basf Plant Science Gmbh | métodos para produzir uma construção de expressão múltipla, para produzir alimento ou produtos alimentìcios, produtos farmacêuticos ou produtos quìmicos, e uma planta ou célula de planta transgênica, construção de expressão múltipla, microorganismo,e, uso de um microorganismo, de uma célula de planta, de um tecido de planta, de um orgão de planta, ou de uma parte de uma planta |
NZ583974A (en) | 2004-05-18 | 2011-11-25 | Intrexon Corp | Methods for dynamic vector assembly of DNA cloning vector plasmids |
US20060064247A1 (en) * | 2004-07-14 | 2006-03-23 | Shao-Min Yuan | Methods and systems for in silico experimental design and for providing a biotechnology product to a customer |
JP2006141320A (ja) * | 2004-11-22 | 2006-06-08 | Invitrogen Japan Kk | 複数の核酸断片をクローニングする方法 |
US20060200878A1 (en) | 2004-12-21 | 2006-09-07 | Linda Lutfiyya | Recombinant DNA constructs and methods for controlling gene expression |
EP2765189A1 (en) | 2004-12-21 | 2014-08-13 | Monsanto Technology LLC | Recombinant DNA constructs and methods for controlling gene expression |
US8314290B2 (en) | 2004-12-21 | 2012-11-20 | Monsanto Technology Llc | Temporal regulation of gene expression by MicroRNAs |
CA2591992A1 (en) | 2004-12-22 | 2006-06-29 | The Salk Institute For Biological Studies | Compositions and methods for producing recombinant proteins |
US7582475B2 (en) | 2005-01-07 | 2009-09-01 | University Of Georgia Research Foundation, Inc. | Vectors and methods for high throughput co-expression |
ATE552343T1 (de) * | 2005-04-19 | 2012-04-15 | Basf Plant Science Gmbh | Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen |
CA2608636C (en) * | 2005-05-17 | 2015-02-10 | Frank Koentgen | Sequential cloning system |
US20060270040A1 (en) * | 2005-05-26 | 2006-11-30 | Conjugon, Inc. | Compositions and methods for treating tissue |
EP1929019A2 (en) * | 2005-09-08 | 2008-06-11 | Chromatin, Inc. | Plants modified with mini-chromosomes |
US9121028B2 (en) * | 2005-09-09 | 2015-09-01 | Monsanto Technology Llc | Selective gene expression in plants |
US20070117179A1 (en) * | 2005-09-27 | 2007-05-24 | Invitrogen Corporation | In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles |
EP3339441A1 (en) | 2005-10-13 | 2018-06-27 | Monsanto Technology LLC | Methods for producing hybrid seed |
US7696335B2 (en) * | 2005-10-13 | 2010-04-13 | Bc Cancer Agency | Kits for multiple non-cross reacting recombination reactions utilizing loxP sequences |
US20070231906A1 (en) * | 2005-10-25 | 2007-10-04 | Invitrogen Corporation | Three Frame cDNA Expression Libraries for Functional Screening of Proteins |
US20100196336A1 (en) | 2006-05-23 | 2010-08-05 | Dongsu Park | Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods |
AU2007290367B2 (en) | 2006-08-31 | 2013-10-31 | Monsanto Technology, Llc | Phased small RNAs |
CN101522901A (zh) | 2006-10-05 | 2009-09-02 | 纳幕尔杜邦公司 | 玉米微rna序列 |
ES2633352T3 (es) | 2006-10-12 | 2017-09-20 | Monsanto Technology Llc | microARN de plantas y procedimientos de uso de los mismos |
EP2113029A4 (en) | 2007-02-09 | 2010-06-02 | Univ Utah Res Found | IN VIVO GENERAL MUTAGENESIS |
AU2008214568B2 (en) | 2007-02-16 | 2013-04-18 | Basf Plant Science Gmbh | Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants |
US8410334B2 (en) | 2007-02-20 | 2013-04-02 | Monsanto Technology Llc | Invertebrate microRNAs |
WO2008106660A2 (en) * | 2007-03-01 | 2008-09-04 | Invitrogen Corporation | Isolated phospholipid-protein particles |
WO2008112972A2 (en) * | 2007-03-15 | 2008-09-18 | Chromatin, Inc. | Centromere sequences and minichromosomes |
US20090042737A1 (en) * | 2007-08-09 | 2009-02-12 | Katz Andrew S | Methods and Devices for Correlated, Multi-Parameter Single Cell Measurements and Recovery of Remnant Biological Material |
US20090280103A1 (en) * | 2008-04-04 | 2009-11-12 | Martin Flueck | Regulation of muscle repair |
BRPI0913657A2 (pt) | 2008-07-01 | 2020-08-04 | Monsanto Technology Llc | constructos de dna recombinante e métodos para a modulação da expressão de um gene-alvo |
WO2011003034A2 (en) | 2009-07-02 | 2011-01-06 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
EP2451960A2 (en) * | 2009-07-09 | 2012-05-16 | Verdezyne, Inc. | Engineered microorganisms with enhanced fermentation activity |
CA2767724A1 (en) | 2009-07-23 | 2011-01-27 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
US8889394B2 (en) * | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
WO2011105429A1 (ja) * | 2010-02-24 | 2011-09-01 | 学校法人日本大学 | 部位特異的組換え方法及びキット |
CN101967475B (zh) * | 2010-06-10 | 2014-01-22 | 复旦大学 | 一种基于位点特异性重组的基因打靶载体快速构建方法 |
CN101921747A (zh) * | 2010-06-10 | 2010-12-22 | 复旦大学 | 一种基于位点特异性重组的克隆方法 |
DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
JP2011078436A (ja) * | 2011-01-27 | 2011-04-21 | National Institute Of Infectious Diseases | プロテアーゼ活性の測定方法 |
US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
US8343752B2 (en) | 2011-05-03 | 2013-01-01 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
CA2841796C (en) | 2011-07-06 | 2021-06-29 | Verdezyne, Inc. | Biological methods for preparing a fatty dicarboxylic acid |
US9465519B2 (en) | 2011-12-21 | 2016-10-11 | Life Technologies Corporation | Methods and systems for in silico experimental designing and performing a biological workflow |
WO2013152220A2 (en) | 2012-04-04 | 2013-10-10 | Life Technologies Corporation | Tal-effector assembly platform, customized services, kits and assays |
DK3064585T3 (da) | 2012-12-12 | 2020-04-27 | Broad Inst Inc | Konstruering og optimering af forbedrede systemer, fremgangsmåder og enzymsammensætninger til sekvensmanipulation |
EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
EP3031921A1 (en) | 2012-12-12 | 2016-06-15 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
SG11201504523UA (en) | 2012-12-12 | 2015-07-30 | Broad Inst Inc | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
MY175858A (en) | 2012-12-19 | 2020-07-14 | Corvay Bioproducts Gmbh | Biological methods for preparing a fatty dicarboxylic acid |
WO2014100504A2 (en) | 2012-12-19 | 2014-06-26 | Verdezyne, Inc. | Biological methods for preparing a fatty dicarboxylic acid |
KR20160056869A (ko) | 2013-06-17 | 2016-05-20 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
CN106062197A (zh) | 2013-06-17 | 2016-10-26 | 布罗德研究所有限公司 | 用于序列操纵的串联指导系统、方法和组合物的递送、工程化和优化 |
EP4245853A3 (en) | 2013-06-17 | 2023-10-18 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
CA2915842C (en) | 2013-06-17 | 2022-11-29 | The Broad Institute, Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
CA2915845A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling diseases and disorders of post mitotic cells |
US9333227B2 (en) | 2013-08-19 | 2016-05-10 | Syngulon Sa. | Controlled growth of microorganisms |
WO2015089462A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for genome editing |
AU2014361784A1 (en) | 2013-12-12 | 2016-06-23 | Massachusetts Institute Of Technology | Delivery, use and therapeutic applications of the CRISPR-Cas systems and compositions for HBV and viral diseases and disorders |
CN106536729A (zh) | 2013-12-12 | 2017-03-22 | 布罗德研究所有限公司 | 使用粒子递送组分靶向障碍和疾病的crispr‑cas系统和组合物的递送、用途和治疗应用 |
WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
EP4219699A1 (en) | 2013-12-12 | 2023-08-02 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
CA2932472A1 (en) | 2013-12-12 | 2015-06-18 | Massachusetts Institute Of Technology | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
EP3129493B1 (en) | 2014-04-09 | 2021-07-07 | The Scripps Research Institute | Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters |
EP3180426B1 (en) | 2014-08-17 | 2019-12-25 | The Broad Institute, Inc. | Genome editing using cas9 nickases |
WO2016049251A1 (en) | 2014-09-24 | 2016-03-31 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling mutations in leukocytes |
WO2016049024A2 (en) | 2014-09-24 | 2016-03-31 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo |
WO2016049163A2 (en) | 2014-09-24 | 2016-03-31 | The Broad Institute Inc. | Use and production of chd8+/- transgenic animals with behavioral phenotypes characteristic of autism spectrum disorder |
EP3208335B1 (en) * | 2014-10-14 | 2020-07-01 | MGI Tech Co., Ltd. | Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent |
US20170247762A1 (en) | 2014-10-27 | 2017-08-31 | The Board Institute Inc. | Compositions, methods and use of synthetic lethal screening |
FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
WO2016094867A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Protected guide rnas (pgrnas) |
WO2016094874A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Escorted and functionalized guides for crispr-cas systems |
EP3230452A1 (en) | 2014-12-12 | 2017-10-18 | The Broad Institute Inc. | Dead guides for crispr transcription factors |
WO2016100974A1 (en) | 2014-12-19 | 2016-06-23 | The Broad Institute Inc. | Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing |
WO2016106236A1 (en) | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
CA2970370A1 (en) | 2014-12-24 | 2016-06-30 | Massachusetts Institute Of Technology | Crispr having or associated with destabilization domains |
WO2016108926A1 (en) | 2014-12-30 | 2016-07-07 | The Broad Institute Inc. | Crispr mediated in vivo modeling and genetic screening of tumor growth and metastasis |
WO2016154046A2 (en) | 2015-03-20 | 2016-09-29 | Verdezyne, Inc. | Biological methods for preparing 3-hydroxypropionic acid |
WO2016205728A1 (en) | 2015-06-17 | 2016-12-22 | Massachusetts Institute Of Technology | Crispr mediated recording of cellular events |
WO2016205749A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
CN109536474A (zh) | 2015-06-18 | 2019-03-29 | 布罗德研究所有限公司 | 降低脱靶效应的crispr酶突变 |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
WO2016205745A2 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Cell sorting |
CA3012631A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
US20190255107A1 (en) | 2015-10-09 | 2019-08-22 | The Brigham And Women's Hospital, Inc. | Modulation of novel immune checkpoint targets |
WO2017070605A1 (en) | 2015-10-22 | 2017-04-27 | The Broad Institute Inc. | Type vi-b crispr enzymes and systems |
US11492670B2 (en) | 2015-10-27 | 2022-11-08 | The Broad Institute Inc. | Compositions and methods for targeting cancer-specific sequence variations |
WO2017075465A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute Inc. | Compositions and methods for evaluating and modulating immune responses by detecting and targeting gata3 |
WO2017075451A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute Inc. | Compositions and methods for evaluating and modulating immune responses by detecting and targeting pou2af1 |
EP3368689B1 (en) | 2015-10-28 | 2020-06-17 | The Broad Institute, Inc. | Composition for modulating immune responses by use of immune cell gene signature |
US12110490B2 (en) | 2015-12-18 | 2024-10-08 | The Broad Institute, Inc. | CRISPR enzymes and systems |
WO2017106767A1 (en) | 2015-12-18 | 2017-06-22 | The Scripps Research Institute | Production of unnatural nucleotides using a crispr/cas9 system |
WO2017184786A1 (en) | 2016-04-19 | 2017-10-26 | The Broad Institute Inc. | Cpf1 complexes with reduced indel activity |
WO2017184768A1 (en) | 2016-04-19 | 2017-10-26 | The Broad Institute Inc. | Novel crispr enzymes and systems |
CA3026110A1 (en) | 2016-04-19 | 2017-11-02 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
US11939571B2 (en) | 2016-06-10 | 2024-03-26 | President And Fellows Of Harvard College | Library-scale engineering of metabolic pathways |
WO2017219027A1 (en) | 2016-06-17 | 2017-12-21 | The Broad Institute Inc. | Type vi crispr orthologs and systems |
US10253316B2 (en) | 2017-06-30 | 2019-04-09 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
DK3475295T3 (da) | 2016-06-24 | 2022-10-24 | Scripps Research Inst | Hidtil ukendt nukleosidtriphosphat-transportør og anvendelser deraf |
WO2018005873A1 (en) | 2016-06-29 | 2018-01-04 | The Broad Institute Inc. | Crispr-cas systems having destabilization domain |
WO2018035250A1 (en) | 2016-08-17 | 2018-02-22 | The Broad Institute, Inc. | Methods for identifying class 2 crispr-cas systems |
EP3500670B1 (en) | 2016-08-17 | 2024-07-10 | The Broad Institute, Inc. | Method for selecting target sequences for guide rna of crispr systems |
CN110114461A (zh) | 2016-08-17 | 2019-08-09 | 博德研究所 | 新型crispr酶和系统 |
WO2018049025A2 (en) | 2016-09-07 | 2018-03-15 | The Broad Institute Inc. | Compositions and methods for evaluating and modulating immune responses |
WO2018067991A1 (en) | 2016-10-07 | 2018-04-12 | The Brigham And Women's Hospital, Inc. | Modulation of novel immune checkpoint targets |
WO2018074979A1 (en) | 2016-10-17 | 2018-04-26 | Nanyang Technological University | Truncated crispr-cas proteins for dna targeting |
US10241477B2 (en) * | 2016-11-02 | 2019-03-26 | Edison Labs, Inc. | Adaptive control methods for buildings with redundant circuitry |
CN108665933B (zh) * | 2016-11-02 | 2020-10-16 | 旺宏电子股份有限公司 | 非易失性存储器元件的操作方法及其应用 |
US10496048B2 (en) * | 2016-11-02 | 2019-12-03 | Edison Labs, Inc. | Switch terminal methods with wiring components secured to circuitry wiring without external live points of contact |
DK3551753T3 (da) | 2016-12-09 | 2022-09-05 | Broad Inst Inc | Diagnostik baseret på crispr-effektorsystem |
JP2020511141A (ja) | 2017-03-15 | 2020-04-16 | ザ・ブロード・インスティテュート・インコーポレイテッド | 新規Cas13bオルソログCRISPR酵素及び系 |
CA3056411A1 (en) | 2017-03-15 | 2018-09-20 | The Broad Institute, Inc. | Crispr effector system based diagnostics for virus detection |
US11174515B2 (en) | 2017-03-15 | 2021-11-16 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
CN108624516B (zh) * | 2017-03-20 | 2022-08-26 | 华东理工大学 | 一种提高发酵细胞中的代谢产物量及制备idms标准品的方法 |
WO2018183908A1 (en) | 2017-03-31 | 2018-10-04 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for treating ovarian tumors |
US20200071773A1 (en) | 2017-04-12 | 2020-03-05 | Massachusetts Eye And Ear Infirmary | Tumor signature for metastasis, compositions of matter methods of use thereof |
BR112019021378A2 (pt) | 2017-04-12 | 2020-05-05 | Massachusetts Inst Technology | ortólogos de crispr tipo vi inovadores e sistemas |
WO2018204777A2 (en) | 2017-05-05 | 2018-11-08 | The Broad Institute, Inc. | Methods for identification and modification of lncrna associated with target genotypes and phenotypes |
US20210278416A1 (en) | 2017-05-09 | 2021-09-09 | The Broad Institute, Inc. | Gut microbiome function predicts response to anti-integrin biologic therapy in inflammatory bowel diseases |
WO2018232195A1 (en) | 2017-06-14 | 2018-12-20 | The Broad Institute, Inc. | Compositions and methods targeting complement component 3 for inhibiting tumor growth |
EP3642334B1 (en) | 2017-06-23 | 2023-12-27 | Inscripta, Inc. | Nucleic acid-guided nucleases |
CA3064601A1 (en) | 2017-06-26 | 2019-01-03 | The Broad Institute, Inc. | Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing |
AU2018300069A1 (en) | 2017-07-11 | 2020-02-27 | Synthorx, Inc. | Incorporation of unnatural nucleotides and methods thereof |
CA3069697A1 (en) | 2017-07-13 | 2019-01-17 | Radici Chimica S.P.A. | Biological methods for modifying cellular carbon flux |
WO2019014581A1 (en) | 2017-07-14 | 2019-01-17 | The Broad Institute, Inc. | METHODS AND COMPOSITIONS FOR MODULATING THE ACTIVITY OF A CYTOTOXIC LYMPHOCYTE |
US12105089B2 (en) | 2017-07-17 | 2024-10-01 | The Broad Institute, Inc. | Cell atlas of the healthy and ulcerative colitis human colon |
CA3071013A1 (en) | 2017-08-03 | 2019-02-07 | Synthorx, Inc. | Cytokine conjugates for the treatment of proliferative and infectious diseases |
US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
EP3684397A4 (en) | 2017-09-21 | 2021-08-18 | The Broad Institute, Inc. | SYSTEMS, METHODS AND COMPOSITIONS FOR TARGETED EDITION OF NUCLEIC ACIDS |
US10435713B2 (en) | 2017-09-30 | 2019-10-08 | Inscripta, Inc. | Flow through electroporation instrumentation |
WO2019071054A1 (en) | 2017-10-04 | 2019-04-11 | The Broad Institute, Inc. | METHODS AND COMPOSITIONS FOR MODIFYING THE FUNCTION AND STRUCTURE OF BUCKLES AND / OR CHROMATIN DOMAINS |
US11547614B2 (en) | 2017-10-31 | 2023-01-10 | The Broad Institute, Inc. | Methods and compositions for studying cell evolution |
EP3710039A4 (en) | 2017-11-13 | 2021-08-04 | The Broad Institute, Inc. | METHODS AND COMPOSITIONS FOR CANCER TREATMENT BY TARGETING THE CLEC2D-KLRB1 PATH |
WO2019113506A1 (en) | 2017-12-07 | 2019-06-13 | The Broad Institute, Inc. | Methods and compositions for multiplexing single cell and single nuclei sequencing |
WO2019121983A1 (en) | 2017-12-19 | 2019-06-27 | Syngulon S.A. | Fermentation process |
US11994512B2 (en) | 2018-01-04 | 2024-05-28 | Massachusetts Institute Of Technology | Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity |
EP3746568B1 (en) | 2018-01-29 | 2023-11-15 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
AU2019241967A1 (en) | 2018-03-29 | 2020-11-19 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
US10968257B2 (en) | 2018-04-03 | 2021-04-06 | The Broad Institute, Inc. | Target recognition motifs and uses thereof |
WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
US10508273B2 (en) | 2018-04-24 | 2019-12-17 | Inscripta, Inc. | Methods for identifying selective binding pairs |
US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
US11957695B2 (en) | 2018-04-26 | 2024-04-16 | The Broad Institute, Inc. | Methods and compositions targeting glucocorticoid signaling for modulating immune responses |
US20210147831A1 (en) | 2018-04-27 | 2021-05-20 | The Broad Institute, Inc. | Sequencing-based proteomics |
US20210371932A1 (en) | 2018-06-01 | 2021-12-02 | Massachusetts Institute Of Technology | Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients |
US12036240B2 (en) | 2018-06-14 | 2024-07-16 | The Broad Institute, Inc. | Compositions and methods targeting complement component 3 for inhibiting tumor growth |
SG11202012785VA (en) | 2018-06-26 | 2021-01-28 | Broad Inst Inc | Crispr double nickase based amplification compositions, systems, and methods |
CA3108767A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US20210308171A1 (en) * | 2018-08-07 | 2021-10-07 | The Broad Institute, Inc. | Methods for combinatorial screening and use of therapeutic targets thereof |
EP3833761A1 (en) | 2018-08-07 | 2021-06-16 | The Broad Institute, Inc. | Novel cas12b enzymes and systems |
US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
WO2020041384A1 (en) | 2018-08-20 | 2020-02-27 | The Broad Institute, Inc. | 3-phenyl-2-cyano-azetidine derivatives, inhibitors of rna-guided nuclease activity |
US20210324357A1 (en) | 2018-08-20 | 2021-10-21 | The Brigham And Women's Hospital, Inc. | Degradation domain modifications for spatio-temporal control of rna-guided nucleases |
US20210317429A1 (en) | 2018-08-20 | 2021-10-14 | The Broad Institute, Inc. | Methods and compositions for optochemical control of crispr-cas9 |
WO2020081149A2 (en) | 2018-08-30 | 2020-04-23 | Inscripta, Inc. | Improved detection of nuclease edited sequences in automated modules and instruments |
WO2020060948A1 (en) | 2018-09-17 | 2020-03-26 | Levadura Biotechnology, Inc. | Production of cannabinoids in yeast using a fatty acid feedstock |
CA3113095A1 (en) | 2018-09-18 | 2020-03-26 | Vnv Newco Inc. | Arc-based capsids and uses thereof |
WO2020072816A1 (en) | 2018-10-03 | 2020-04-09 | The Broad Institute, Inc. | Crispr effector system based diagnostics for hemorrhagic fever detection |
US20220401460A1 (en) | 2018-10-10 | 2022-12-22 | Dana-Farber Cancer Institute, Inc. | Modulating resistance to bcl-2 inhibitors |
WO2020077236A1 (en) | 2018-10-12 | 2020-04-16 | The Broad Institute, Inc. | Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues |
CN113474456A (zh) | 2018-11-14 | 2021-10-01 | 博德研究所 | 基于crispr系统的液滴诊断系统和方法 |
CA3119971A1 (en) | 2018-11-14 | 2020-05-22 | Pardis SABETI | Multiplexing highly evolving viral variants with sherlock |
WO2020124050A1 (en) | 2018-12-13 | 2020-06-18 | The Broad Institute, Inc. | Tiled assays using crispr-cas based detection |
CA3124110A1 (en) | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
US11739156B2 (en) | 2019-01-06 | 2023-08-29 | The Broad Institute, Inc. Massachusetts Institute of Technology | Methods and compositions for overcoming immunosuppression |
WO2020146319A1 (en) * | 2019-01-09 | 2020-07-16 | Asimov, Inc. | Compositions of polynucleic acid vectors and uses thereof |
BR112021014415A2 (pt) | 2019-02-06 | 2021-09-21 | Synthorx, Inc. | Conjugados de il-2 e métodos de uso dos mesmos |
US20220154258A1 (en) | 2019-03-14 | 2022-05-19 | The Broad Institute, Inc. | Crispr effector system based multiplex diagnostics |
US20220177863A1 (en) | 2019-03-18 | 2022-06-09 | The Broad Institute, Inc. | Type vii crispr proteins and systems |
WO2020206046A1 (en) | 2019-04-01 | 2020-10-08 | The Broad Institute, Inc. | Methods and compositions for cell therapy |
WO2020232271A1 (en) | 2019-05-14 | 2020-11-19 | The Broad Institute, Inc. | Compositions and methods for targeting multinucleated cells |
US20220220469A1 (en) | 2019-05-20 | 2022-07-14 | The Broad Institute, Inc. | Non-class i multi-component nucleic acid targeting systems |
WO2020243661A1 (en) | 2019-05-31 | 2020-12-03 | The Broad Institute, Inc. | Methods for treating metabolic disorders by targeting adcy5 |
WO2020252262A1 (en) | 2019-06-14 | 2020-12-17 | The Scripps Research Institute | Reagents and methods for replication, transcription, and translation in semi-synthetic organisms |
US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
EP3986909A4 (en) | 2019-06-21 | 2023-08-02 | Inscripta, Inc. | GENOME-WIDE RATIONAL DESIGNED MUTATIONS LEADING TO INCREASED LYSINE PRODUCTION IN E. COLI |
US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
US20220333208A1 (en) | 2019-09-03 | 2022-10-20 | The Broad Institute, Inc. | Crispr effector system based multiplex cancer diagnostics |
EP4028064A1 (en) | 2019-09-12 | 2022-07-20 | The Broad Institute, Inc. | Engineered adeno-associated virus capsids |
CN114616336A (zh) | 2019-09-20 | 2022-06-10 | 博德研究所 | 用于将货物递送至靶细胞的组合物和方法 |
US11981922B2 (en) | 2019-10-03 | 2024-05-14 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment |
US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
CA3157061A1 (en) | 2020-01-27 | 2021-08-05 | Christian SILTANEN | Electroporation modules and instrumentation |
US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
CN116917478A (zh) | 2020-12-22 | 2023-10-20 | 亥姆霍兹慕尼黑中心-德国健康与环境研究中心(有限公司) | Crispr/cas13在rna病毒和/或细菌诱导的疾病的治疗中的应用 |
WO2023004367A2 (en) | 2021-07-20 | 2023-01-26 | The Broad Institute, Inc. | Engineered targeting compositions for endothelial cells of the central nervous system vasculature and methods of use thereof |
CA3237300A1 (en) | 2021-11-01 | 2023-05-04 | Tome Biosciences, Inc. | Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo |
IL313765A (en) | 2021-12-22 | 2024-08-01 | Tome Biosciences Inc | Joint provision of a gene editor structure and a donor template |
WO2023196818A1 (en) | 2022-04-04 | 2023-10-12 | The Regents Of The University Of California | Genetic complementation compositions and methods |
WO2023200770A1 (en) | 2022-04-12 | 2023-10-19 | Inscripta, Inc. | Curing for iterative nucleic acid-guided nuclease editing |
WO2023205744A1 (en) | 2022-04-20 | 2023-10-26 | Tome Biosciences, Inc. | Programmable gene insertion compositions |
WO2023215831A1 (en) | 2022-05-04 | 2023-11-09 | Tome Biosciences, Inc. | Guide rna compositions for programmable gene insertion |
WO2023225564A1 (en) | 2022-05-18 | 2023-11-23 | The Broad Institute, Inc. | Engineered viral capsids with increased stability and methods of use thereof |
WO2023225670A2 (en) | 2022-05-20 | 2023-11-23 | Tome Biosciences, Inc. | Ex vivo programmable gene insertion |
WO2024016003A2 (en) | 2022-07-14 | 2024-01-18 | The Broad Institute, Inc. | Aav capsids that enable cns-wide gene delivery through interactions with the transferrin receptor |
WO2024020587A2 (en) | 2022-07-22 | 2024-01-25 | Tome Biosciences, Inc. | Pleiopluripotent stem cell programmable gene insertion |
CN116024234B (zh) * | 2022-12-12 | 2023-07-21 | 南京林业大学 | 一种杨树壳针孢菌效应蛋白SmCSEP3及其应用 |
WO2024138194A1 (en) | 2022-12-22 | 2024-06-27 | Tome Biosciences, Inc. | Platforms, compositions, and methods for in vivo programmable gene insertion |
WO2024163842A2 (en) | 2023-02-03 | 2024-08-08 | The Broad Institute, Inc. | Delivering genes to the brain endothelium to treat lysosomal storage disorder-derived neuropathology |
WO2024168265A1 (en) | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Aav delivery of rna guided recombination system |
WO2024168253A1 (en) | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Delivery of an rna guided recombination system |
Family Cites Families (224)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5844072A (en) | 1992-05-26 | 1998-12-01 | University Of California | Antibiotic cryptdin peptides and methods of their use |
US4372745A (en) * | 1979-12-19 | 1983-02-08 | Electro-Nucleonics, Inc. | Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer |
US5097097A (en) * | 1980-07-30 | 1992-03-17 | Abbott Laboratories | Triazinylaminofluoresceins |
US4626505A (en) * | 1984-02-24 | 1986-12-02 | E. I. Du Pont De Nemours And Company | Selectable markers for yeast transformation |
US4673640A (en) | 1984-04-30 | 1987-06-16 | Biotechnica International, Inc. | Regulated protein production using site-specific recombination |
US4808537A (en) * | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
US4855231A (en) * | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
US4743546A (en) | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
US4797368A (en) * | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
DE3688920D1 (de) * | 1985-07-03 | 1993-09-30 | Genencor Int | Hybride Polypeptide und Verfahren zu deren Herstellung. |
US6121043A (en) * | 1985-09-06 | 2000-09-19 | Syntro Corporation | Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome |
CA1293460C (en) * | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US4959217A (en) * | 1986-05-22 | 1990-09-25 | Syntex (U.S.A.) Inc. | Delayed/sustained release of macromolecules |
US5079352A (en) * | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
US4987071A (en) * | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
US4795699A (en) * | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
DE3876327D1 (de) * | 1987-07-21 | 1993-01-14 | Du Pont Merck Pharma | Verfahren fuer die herstellung von in tierischen zellen stabilen und lebensfaehigen rekombinanten viralen vektoren. |
US5204244A (en) * | 1987-10-27 | 1993-04-20 | Oncogen | Production of chimeric antibodies by homologous recombination |
JPH03503482A (ja) | 1988-02-12 | 1991-08-08 | コモンウェルス・サイエンティフィック・アンド・インダストリアル・リサーチ・オーガナイゼーション | ポックスウイルスベクター |
US5378618A (en) * | 1988-04-15 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Vitro headful packaging system for cloning DNA fragments as large as 95kb |
US5334375A (en) * | 1988-12-29 | 1994-08-02 | Colgate Palmolive Company | Antibacterial antiplaque oral composition |
DE3901681A1 (de) * | 1989-01-21 | 1990-07-26 | Behringwerke Ag | Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung |
CA2012983A1 (en) * | 1989-03-27 | 1990-09-27 | Sydney Brenner | Process for nucleic acid detection by binary amplification |
US5102797A (en) * | 1989-05-26 | 1992-04-07 | Dna Plant Technology Corporation | Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system |
US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
US5744101A (en) * | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US6346413B1 (en) * | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
US6309822B1 (en) * | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
JPH05503626A (ja) | 1989-08-22 | 1993-06-17 | イー・アイ・デュポン・ドゥ・ヌムール・アンド・カンパニー | 95kbの大きさのdna断片をクローニングするための生体外ヘッドフルパッケージング系 |
NO904633L (no) | 1989-11-09 | 1991-05-10 | Molecular Diagnostics Inc | Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe. |
US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
EP0681025B1 (en) * | 1990-01-08 | 2001-05-09 | Stratagene | Novel cloning host organisms |
US5283173A (en) * | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
US5082784A (en) * | 1990-03-20 | 1992-01-21 | Life Technologies, Inc. | Cloned kpni restriction-modification system |
US5192675A (en) * | 1990-03-20 | 1993-03-09 | Life Technologies, Inc. | Cloned KpnI restriction-modification system |
WO1991016427A1 (en) * | 1990-04-24 | 1991-10-31 | Stratagene | Methods for phenotype creation from multiple gene populations |
US5451513A (en) * | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
US5098839A (en) * | 1990-05-10 | 1992-03-24 | New England Biolabs, Inc. | Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same |
AU665190B2 (en) | 1990-07-10 | 1995-12-21 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
US5179015A (en) * | 1990-07-23 | 1993-01-12 | New England Biolabs, Inc. | Heterospecific modification as a means to clone restriction genes |
DE69133389T2 (de) | 1990-09-27 | 2005-06-02 | Invitrogen Corp., Carlsbad | Direkte Klonierung von PCR amplifizierten Nukleinsäuren |
US5227288A (en) | 1990-10-01 | 1993-07-13 | Blattner Frederick R | DNA sequencing vector with reversible insert |
FR2670502B1 (fr) | 1990-12-13 | 1994-09-16 | Eurolysine | Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee. |
US5286632A (en) * | 1991-01-09 | 1994-02-15 | Jones Douglas H | Method for in vivo recombination and mutagenesis |
US5348788A (en) * | 1991-01-30 | 1994-09-20 | Interpore Orthopaedics, Inc. | Mesh sheet with microscopic projections and holes |
WO1992015694A1 (en) * | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
DE69230142T2 (de) | 1991-05-15 | 2000-03-09 | Cambridge Antibody Technology Ltd. | Verfahren zur herstellung von spezifischen bindungspaargliedern |
US5858657A (en) | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
US5962255A (en) * | 1992-03-24 | 1999-10-05 | Cambridge Antibody Technology Limited | Methods for producing recombinant vectors |
AU681572B2 (en) | 1991-10-21 | 1997-09-04 | Med Immune, Inc. | Bacterial expression vectors containing DNA encoding secretion signals of lipoproteins |
GB9123929D0 (en) | 1991-11-11 | 1992-01-02 | Wellcome Found | Novel expression system |
ZA929351B (en) * | 1991-12-11 | 1993-06-04 | Igen Inc | Electrochemiluminescent label for DNA assays. |
EP0626999A4 (en) | 1992-01-24 | 1997-04-09 | Life Technologies Inc | Modulation of enzyme activities in the -i(in vivo) cloning of dna. |
ES2157217T3 (es) | 1992-02-26 | 2001-08-16 | Zeneca Mogen B V | Cepas de agrobacterium capaces de recombinacion especifica para el sitio. |
JPH05236997A (ja) | 1992-02-28 | 1993-09-17 | Hitachi Ltd | ポリヌクレオチド捕捉用チップ |
EP0656941B1 (en) * | 1992-03-24 | 2005-06-01 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
US5733743A (en) | 1992-03-24 | 1998-03-31 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
US5286393A (en) * | 1992-04-15 | 1994-02-15 | Jet-Lube, Inc. | Coating and bonding composition |
US5285173A (en) | 1992-07-13 | 1994-02-08 | Analog Devices, Inc. | Signal-controlled ring oscillator with delay cells having constant gain with change in frequency |
US7176029B2 (en) * | 1992-07-31 | 2007-02-13 | Universite Libre De Bruxelles | Cloning and/or sequencing vector |
BE1006085A3 (fr) | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
US5348886A (en) * | 1992-09-04 | 1994-09-20 | Monsanto Company | Method of producing recombinant eukaryotic viruses in bacteria |
US6225121B1 (en) * | 1992-09-14 | 2001-05-01 | Institute Of Molecular Biology And Biotechnology/Forth | Eukaryotic transposable element |
CZ285533B6 (cs) | 1992-10-02 | 1999-08-11 | Lonza Ag | DNA-fragmenty, plasmidy a mikroorganismy obsahující tyto DNA-fragmenty a plasmidy a způsob biotechnologické výroby biotinu |
WO1994013804A1 (en) | 1992-12-04 | 1994-06-23 | Medical Research Council | Multivalent and multispecific binding proteins, their manufacture and use |
US5334575A (en) * | 1992-12-17 | 1994-08-02 | Eastman Kodak Company | Dye-containing beads for laser-induced thermal dye transfer |
US5527695A (en) | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
GB9302798D0 (en) * | 1993-02-12 | 1993-03-31 | Medical Res Council | Improvements in or relating to cloning dna |
FR2705686B1 (fr) * | 1993-05-28 | 1995-08-18 | Transgene Sa | Nouveaux adénovirus défectifs et lignées de complémentation correspondantes. |
US5834256A (en) * | 1993-06-11 | 1998-11-10 | Cell Genesys, Inc. | Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells |
EP1413585A3 (en) | 1993-06-15 | 2004-08-04 | E.I. Du Pont De Nemours And Company | Novel, recombinantly produced spider silk analogs |
US6140087A (en) * | 1993-06-24 | 2000-10-31 | Advec, Inc. | Adenovirus vectors for gene therapy |
US6120764A (en) * | 1993-06-24 | 2000-09-19 | Advec, Inc. | Adenoviruses for control of gene expression |
US5919676A (en) | 1993-06-24 | 1999-07-06 | Advec, Inc. | Adenoviral vector system comprising Cre-loxP recombination |
EP0632054A1 (en) | 1993-06-28 | 1995-01-04 | European Molecular Biology Laboratory | Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins |
US5843744A (en) | 1993-07-08 | 1998-12-01 | Ecogen Inc. | Bacillus thuringiensis Tn5401 proteins |
US5441884A (en) | 1993-07-08 | 1995-08-15 | Ecogen Inc. | Bacillus thuringiensis transposon TN5401 |
US5470727A (en) | 1993-12-21 | 1995-11-28 | Celtrix Pharmaceuticals, Inc. | Chromosomal expression of non-bacterial genes in bacterial cells |
FR2715940B1 (fr) * | 1994-02-10 | 1996-04-26 | Pasteur Merieux Serums Vacc | Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus. |
US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5492841A (en) * | 1994-02-18 | 1996-02-20 | E. I. Du Pont De Nemours And Company | Quaternary ammonium immunogenic conjugates and immunoassay reagents |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
WO1995032225A1 (en) | 1994-05-23 | 1995-11-30 | The Salk Institute For Biological Studies | Method for site-specific integration of nucleic acids and related products |
US5723765A (en) | 1994-08-01 | 1998-03-03 | Delta And Pine Land Co. | Control of plant gene expression |
US5589618A (en) * | 1994-09-01 | 1996-12-31 | University Of Florida | Materials and methods for increasing corn seed weight |
US5601980A (en) * | 1994-09-23 | 1997-02-11 | Hewlett-Packard Company | Manufacturing method and apparatus for biological probe arrays using vision-assisted micropipetting |
US5605802A (en) * | 1994-09-30 | 1997-02-25 | The Salk Institute For Biological Studies | Method for identifying agents which block infection of cells by HIV |
US6015668A (en) * | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
FR2727689A1 (fr) * | 1994-12-01 | 1996-06-07 | Transgene Sa | Nouveau procede de preparation d'un vecteur viral |
US5766891A (en) * | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US5981177A (en) * | 1995-01-25 | 1999-11-09 | Demirjian; David C. | Protein fusion method and constructs |
FR2731014B1 (fr) | 1995-02-23 | 1997-03-28 | Rhone Poulenc Rorer Sa | Molecules d'adn, preparation et utilisation en therapie genique |
US5599695A (en) * | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
US5814300A (en) * | 1995-03-03 | 1998-09-29 | Cephalon, Inc. | Gene-targeted non-human mammals deficient in the SOD-1 gene |
US5695971A (en) * | 1995-04-07 | 1997-12-09 | Amresco | Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production |
US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
DE69623057T2 (de) | 1995-06-07 | 2003-03-27 | Invitrogen Corp., Carlsbad | Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte |
US6720140B1 (en) * | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US5877021A (en) * | 1995-07-07 | 1999-03-02 | Ribozyme Pharmaceuticals, Inc. | B7-1 targeted ribozymes |
US6455254B1 (en) * | 1995-07-18 | 2002-09-24 | Diversa Corporation | Sequence based screening |
FR2737222B1 (fr) * | 1995-07-24 | 1997-10-17 | Transgene Sa | Nouveaux vecteurs viraux et lignee pour la therapie genique |
WO1997006265A2 (en) | 1995-08-07 | 1997-02-20 | The Perkin-Elmer Corporation | Recombinant clone selection system |
US5801030A (en) * | 1995-09-01 | 1998-09-01 | Genvec, Inc. | Methods and vectors for site-specific recombination |
AUPN523995A0 (en) | 1995-09-05 | 1995-09-28 | Crc For Biopharmaceutical Research Pty Ltd | Method for producing phage display vectors |
DK0854933T3 (da) * | 1995-09-07 | 2003-07-21 | Novozymes As | Fagfremvisning for detergentenzym aktivitet |
US20030027296A1 (en) * | 1995-09-08 | 2003-02-06 | Deb K. Chatterjee | Cloned dna polymerases from thermotoga maritima and mutants thereof |
US6013516A (en) * | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
WO1997013401A1 (en) * | 1995-10-13 | 1997-04-17 | Purdue Research Foundation | Method for the production of hybrid plants |
WO1997014789A2 (en) * | 1995-10-17 | 1997-04-24 | Genencor International, Inc. | Enzymatic array and process of making same |
US5695917A (en) * | 1995-11-22 | 1997-12-09 | Eastman Kodak Company | Combination of yellow filter dye and 4-equivalent pyrazolone magenta coupler |
US6713279B1 (en) * | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
US6352842B1 (en) * | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
US6361974B1 (en) * | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
US6489145B1 (en) * | 1996-07-09 | 2002-12-03 | Diversa Corporation | Method of DNA shuffling |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6740506B2 (en) * | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US6479258B1 (en) * | 1995-12-07 | 2002-11-12 | Diversa Corporation | Non-stochastic generation of genetic vaccines |
US6358709B1 (en) * | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6156497A (en) * | 1996-01-05 | 2000-12-05 | Genetic Therapy, Inc. | Recombinase-mediated generation of adenoviral vectors |
US6228646B1 (en) * | 1996-03-07 | 2001-05-08 | The Regents Of The University Of California | Helper-free, totally defective adenovirus for gene therapy |
US6013440A (en) * | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
JP3346976B2 (ja) * | 1996-03-18 | 2002-11-18 | 理化学研究所 | 完全長cDNAライブラリーの作成方法 |
US5721435A (en) * | 1996-04-09 | 1998-02-24 | Hewlett Packard Company | Methods and apparatus for measuring optical properties of biological and chemical substances |
US5928914A (en) * | 1996-06-14 | 1999-07-27 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Methods and compositions for transforming cells |
US5710248A (en) | 1996-07-29 | 1998-01-20 | University Of Iowa Research Foundation | Peptide tag for immunodetection and immunopurification |
AU722375B2 (en) | 1996-09-06 | 2000-08-03 | Trustees Of The University Of Pennsylvania, The | Methods using cre-lox for production of recombinant adeno-associated viruses |
US6025192A (en) * | 1996-09-20 | 2000-02-15 | Cold Spring Harbor Laboratory | Modified retroviral vectors |
JP3385032B2 (ja) * | 1996-09-21 | 2003-03-10 | ヴィロメディカ パシフィック リミテッド | 遺伝子治療のための改善されたレトロウイルスベクター |
US6271436B1 (en) * | 1996-10-11 | 2001-08-07 | The Texas A & M University System | Cells and methods for the generation of transgenic pigs |
US5962303A (en) * | 1996-10-15 | 1999-10-05 | Smithkline Beecham Corporation | Topoisomerase III |
US6066778A (en) * | 1996-11-06 | 2000-05-23 | The Regents Of The University Of Michigan | Transgenic mice expressing APC resistant factor V |
ATE550429T1 (de) * | 1996-11-20 | 2012-04-15 | Crucell Holland Bv | Adenovirus-zusammensetzungen erhältlich durch ein verbessertes produktions- und reinigungsverfahren |
US6303301B1 (en) * | 1997-01-13 | 2001-10-16 | Affymetrix, Inc. | Expression monitoring for gene function identification |
US6410220B1 (en) | 1997-02-28 | 2002-06-25 | Nature Technology Corp | Self-assembling genes, vectors and uses thereof |
US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
US5948653A (en) * | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
DE19720839B4 (de) | 1997-05-17 | 2007-08-16 | Dirk Dr. Schindelhauer | Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT |
US5876946A (en) * | 1997-06-03 | 1999-03-02 | Pharmacopeia, Inc. | High-throughput assay |
US5856144A (en) * | 1997-06-18 | 1999-01-05 | Novagen, Inc. | Direct cloning of DNA fragments |
US5989872A (en) | 1997-08-12 | 1999-11-23 | Clontech Laboratories, Inc. | Methods and compositions for transferring DNA sequence information among vectors |
US6140086A (en) | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
GB9717953D0 (en) * | 1997-08-22 | 1997-10-29 | Smithkline Beecham Biolog | Vaccine |
US7135608B1 (en) | 1997-08-28 | 2006-11-14 | The Salk Institute For Biological Studies | Site-specific recombination in eukaryotes and constructs useful therefor |
US5888795A (en) * | 1997-09-09 | 1999-03-30 | Becton, Dickinson And Company | Thermostable uracil DNA glycosylase and methods of use |
US6897066B1 (en) * | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
ATE341621T1 (de) * | 1997-10-24 | 2006-10-15 | Invitrogen Corp | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
JP4206154B2 (ja) | 1997-11-13 | 2009-01-07 | 大日本住友製薬株式会社 | 変異型loxP配列とその応用 |
ATE317440T1 (de) * | 1997-11-18 | 2006-02-15 | Pioneer Hi Bred Int | Neuartige methode zur integration von fremd-dna ins eukaryotische genom |
US5874259A (en) | 1997-11-21 | 1999-02-23 | Wisconsin Alumni Research Foundation | Conditionally amplifiable BAC vector |
ES2337027T3 (es) * | 1997-12-05 | 2010-04-20 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli. |
US5994136A (en) * | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
DE69822206T2 (de) * | 1997-12-19 | 2005-02-17 | Affymetrix, Inc., Santa Clara | Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen |
US6506559B1 (en) * | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
US6436707B1 (en) * | 1998-03-27 | 2002-08-20 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
US6080576A (en) * | 1998-03-27 | 2000-06-27 | Lexicon Genetics Incorporated | Vectors for gene trapping and gene activation |
US6024192A (en) * | 1998-04-20 | 2000-02-15 | Griffin; Maurice B. | Ladder vise |
WO1999055851A2 (en) | 1998-04-28 | 1999-11-04 | Novartis Ag | Site-directed transformation of plants |
US6197584B1 (en) * | 1998-05-01 | 2001-03-06 | Isis Pharmaceuticals, Inc. | Antisense modulation of CD40 expression |
US6413776B1 (en) * | 1998-06-12 | 2002-07-02 | Galapagos Geonomics N.V. | High throughput screening of gene function using adenoviral libraries for functional genomics applications |
US6031094A (en) * | 1998-07-23 | 2000-02-29 | The Regents Of The University Of California | Beta-lactam substrates and uses thereof |
GB9816761D0 (en) * | 1998-07-31 | 1998-09-30 | Phogen Limited | Herpesvirus preparations and their uses |
US6190908B1 (en) * | 1998-08-12 | 2001-02-20 | The Scripps Research Institute | Modulation of polypeptide display on modified filamentous phage |
AU5584999A (en) * | 1998-08-28 | 2000-03-21 | Invitrogen Corporation | System for the rapid manipulation of nucleic acid sequences |
WO2000029000A1 (en) | 1998-11-13 | 2000-05-25 | Life Technologies, Inc. | Compositions and methods for recombinational cloning of nucleic acid molecules |
NZ525134A (en) | 1999-03-02 | 2004-09-24 | Invitrogen Corp | Compositions and methods for use in recombinational cloning of nucleic acids |
JP2002537800A (ja) * | 1999-03-02 | 2002-11-12 | インビトロゲン・コーポレーション | 毒性遺伝子に対して耐性のある細胞およびその使用 |
CA2364997A1 (en) * | 1999-03-05 | 2000-09-08 | Maxygen, Inc. | Encryption of traits using split gene sequences |
FI105584B (fi) * | 1999-03-30 | 2000-09-15 | Valmet Corp | Menetelmä paperi- tai kartonkikoneen telan valmistamiseksi ja paperi- tai kartonkikoneen tela |
EP1169458A2 (en) | 1999-04-06 | 2002-01-09 | Oklahoma Medical Research Foundation | Method for selecting recombinase variants with altered specificity |
US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
AU781628B2 (en) * | 1999-07-14 | 2005-06-02 | Clontech Laboratories, Inc. | Recombinase-based methods for producing expression vectors and compositions for use in practicing the same |
WO2001011058A1 (en) | 1999-08-09 | 2001-02-15 | Monsanto Technology Llc | Novel cloning methods and vectors |
DE19941186A1 (de) * | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
US20020006664A1 (en) * | 1999-09-17 | 2002-01-17 | Sabatini David M. | Arrayed transfection method and uses related thereto |
WO2001020015A1 (en) * | 1999-09-17 | 2001-03-22 | Whitehead Institute For Biomedical Research | Reverse transfection method |
CN1757724B (zh) | 1999-12-10 | 2014-06-11 | 茵维特罗根公司 | 具有独特特异性的多个重组位点在重组克隆中的用途 |
JP2002076051A (ja) * | 2000-09-01 | 2002-03-15 | Nec Corp | 半導体装置のボンディングパッド構造及びボンディング方法 |
EP1504119A2 (en) * | 2000-02-25 | 2005-02-09 | Stratagene California | Method for ligating nucleic acids and molecular cloning |
AU2001245793A1 (en) * | 2000-03-16 | 2001-09-24 | Cold Spring Harbor Laboratory | Methods and compositions for rna interference |
US20020025561A1 (en) * | 2000-04-17 | 2002-02-28 | Hodgson Clague Pitman | Vectors for gene-self-assembly |
JP4757369B2 (ja) * | 2000-05-08 | 2011-08-24 | パナソニック株式会社 | 角形アルカリ蓄電池、並びにこれを用いた単位電池及び組電池 |
US7244560B2 (en) * | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US20020068290A1 (en) * | 2000-05-31 | 2002-06-06 | Timur Yarovinsky | Topoisomerase activated oligonucleotide adaptors and uses therefor |
GB0016747D0 (en) | 2000-07-08 | 2000-08-30 | Johnson Matthey Plc | Ink |
ATE315086T1 (de) * | 2000-08-21 | 2006-02-15 | Invitrogen Corp | Methoden und reagenzien für molekulares klonieren |
WO2003103600A2 (en) | 2002-06-05 | 2003-12-18 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
JP2004537712A (ja) * | 2000-10-18 | 2004-12-16 | バーチャル・アレイズ・インコーポレーテッド | 多重細胞分析システム |
US20060008817A1 (en) * | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
WO2002083910A2 (en) * | 2001-01-18 | 2002-10-24 | Clontech Laboratories, Inc. | Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same |
NZ527679A (en) * | 2001-02-07 | 2005-07-29 | Invitrogen Corp | Nucleic acid Ter-(termination sequence) sites and Ter-binding proteins or RTPs in vitro and in vivo or combinations thereof |
JP2004531259A (ja) | 2001-04-19 | 2004-10-14 | インヴィトロジェン コーポレーション | 核酸分子の組換えクローニングのための組成物および方法 |
CA2448505A1 (en) * | 2001-05-21 | 2002-11-28 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
US20030032203A1 (en) * | 2001-07-10 | 2003-02-13 | Sabatini David M. | Small molecule microarrays |
US6838285B2 (en) * | 2001-09-18 | 2005-01-04 | Becton Dickinson | Site specific recombinase based method for producing adenoviral vectors |
US20030135888A1 (en) * | 2001-09-26 | 2003-07-17 | Tong Zhu | Genes that are modulated by posttranscriptional gene silencing |
JP4210769B2 (ja) | 2001-11-22 | 2009-01-21 | 独立行政法人産業技術総合研究所 | Gatewayエントリークローンの作製方法 |
US7294504B1 (en) * | 2001-12-27 | 2007-11-13 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods and compositions for DNA mediated gene silencing |
AU2003209054A1 (en) * | 2002-02-07 | 2003-09-02 | Discovery Genomics, Inc. | Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation and methods of use thereof |
US7175806B2 (en) * | 2002-03-15 | 2007-02-13 | Deal Jeffery L | C-band disinfector |
US20040132133A1 (en) * | 2002-07-08 | 2004-07-08 | Invitrogen Corporation | Methods and compositions for the production, identification and purification of fusion proteins |
AU2003253992A1 (en) * | 2002-07-18 | 2004-02-09 | Robert P. Bennett | Viral vectors containing recombination sites |
AU2003257109A1 (en) | 2002-08-05 | 2004-02-23 | Invitrogen Corporation | Compositions and methods for molecular biology |
US20040253620A1 (en) * | 2003-04-30 | 2004-12-16 | Invitrogen Corporation | Use of site specific recombination to prepare molecular markers |
EP1644538A4 (en) * | 2003-06-26 | 2006-11-08 | Invitrogen Corp | METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS |
EP1660631B1 (en) | 2003-08-01 | 2013-04-24 | Life Technologies Corporation | Compositions and methods for purifying short rna molecules |
US20050069929A1 (en) * | 2003-08-08 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for seamless cloning of nucleic acid molecules |
US20050176065A1 (en) * | 2003-10-22 | 2005-08-11 | Invitrogen Corporation | Target sequences for synthetic molecules |
EP1697534B1 (en) * | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
-
1998
- 1998-10-26 AT AT98955110T patent/ATE341621T1/de not_active IP Right Cessation
- 1998-10-26 NZ NZ533288A patent/NZ533288A/en unknown
- 1998-10-26 EP EP98955110A patent/EP1025217B1/en not_active Expired - Lifetime
- 1998-10-26 NZ NZ520579A patent/NZ520579A/en unknown
- 1998-10-26 CA CA002307016A patent/CA2307016A1/en not_active Abandoned
- 1998-10-26 IL IL13577698A patent/IL135776A0/xx unknown
- 1998-10-26 NZ NZ504214A patent/NZ504214A/xx unknown
- 1998-10-26 AU AU11995/99A patent/AU752704B2/en not_active Ceased
- 1998-10-26 CN CNB988116952A patent/CN100342008C/zh not_active Expired - Lifetime
- 1998-10-26 WO PCT/US1998/022589 patent/WO1999021977A1/en active IP Right Grant
- 1998-10-26 DE DE69836092T patent/DE69836092T2/de not_active Expired - Lifetime
- 1998-10-26 JP JP2000518069A patent/JP4303418B2/ja not_active Expired - Fee Related
-
1999
- 1999-04-22 US US09/296,280 patent/US6277608B1/en not_active Expired - Lifetime
-
2001
- 2001-02-09 HK HK01100940A patent/HK1030019A1/xx not_active IP Right Cessation
- 2001-07-17 HK HK01104990A patent/HK1034287A1/xx not_active IP Right Cessation
- 2001-07-19 US US09/907,900 patent/US20020172997A1/en not_active Abandoned
- 2001-07-19 US US09/907,719 patent/US20020192819A1/en not_active Abandoned
- 2001-11-02 US US09/985,448 patent/US20030157716A1/en not_active Abandoned
-
2002
- 2002-11-21 US US10/300,892 patent/US20030175970A1/en not_active Abandoned
-
2003
- 2003-10-08 US US10/680,316 patent/US20040063207A1/en not_active Abandoned
-
2004
- 2004-04-02 US US10/815,730 patent/US20040171156A1/en not_active Abandoned
- 2004-04-08 US US10/820,133 patent/US20040171157A1/en not_active Abandoned
- 2004-07-19 US US10/893,235 patent/US7408049B2/en not_active Expired - Fee Related
- 2004-08-19 US US10/921,265 patent/US20050009091A1/en not_active Abandoned
-
2008
- 2008-09-09 US US12/207,382 patent/US20090186385A1/en not_active Abandoned
- 2008-09-19 US US12/233,836 patent/US7714116B2/en not_active Expired - Fee Related
-
2009
- 2009-02-03 JP JP2009022278A patent/JP2009136297A/ja not_active Withdrawn
- 2009-07-06 US US12/497,915 patent/US20100267120A1/en not_active Abandoned
- 2009-07-06 US US12/497,832 patent/US20100267119A1/en not_active Abandoned
-
2013
- 2013-06-28 US US13/931,804 patent/US20140004567A1/en not_active Abandoned
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IL135776A0 (en) | Recombinational cloning using nucleic acids having recombination sites | |
NZ312332A (en) | Recombinational cloning using engineered recombination sites | |
MX9605716A (es) | Metodos y composiciones para el tratamiento de restenosis y cancer usando ribozimas. | |
AU4981101A (en) | Subtilisin variants | |
EP1173460A4 (en) | PREPARATIONS AND METHODS FOR USE IN RECOMBINATORY CLONING OF NUCLEIC ACIDS | |
AU3246300A (en) | Polypeptides having galactose oxidase activity and nucleic acids encoding same | |
GB2320716B (en) | Improvements in or relating to starch content of plants | |
IL193919A0 (en) | DcR3 POLYPEPTIDE A TNFR HOMOLOG | |
MXPA03002572A (es) | Secuencias de nucleotidos que intervienen en la fertilidad de los machos y metodo de su uso. | |
DE69833784D1 (de) | Polypeptide mit aminopeptidaseaktivität und für diese kodierende nukleinsäuren | |
AU7381098A (en) | Polypeptides having prolyl pipeptidyl aminopeptidase activity and nucleic acids encoding same | |
ATE450603T1 (de) | Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren | |
IL135911A0 (en) | Dna 19355 polypeptide, a tumor necrosis factor homolog | |
AU7489098A (en) | Polypeptides having aminopeptidase activity and nucleic acids encoding same | |
EP1012603A4 (en) | FUNCTIONAL FRAGMENTS OF THE HIV-1 VPR PROTEIN AND METHODS OF USE | |
WO2003076561A3 (en) | Recombinase fusion protein with enhanced cellular uptake | |
MXPA03006984A (es) | Secuencia de nucleotidos que median la fertilidad masculina y metodo de uso de la misma. | |
PL313445A1 (en) | Grb3-3 gene, variants thereof and their use | |
AU3162097A (en) | Retinoid metabolizing protein | |
MXPA03004539A (es) | Luciferasas aisladas y uso de las mismas. | |
AU2002211843A1 (en) | 2.5-diketo-d-gluconic acid reductases and methods of use | |
DE69737597D1 (de) | Vektoren zur inhibierung von viralem und tumorwachstum | |
AU1526099A (en) | Polypeptides having 5-aminolevulinic acid synthase activity and nucleic acids encoding same | |
AU7645400A (en) | Polypeptides having glucanotransferase activity and nucleic acids encoding same | |
ES2164545A1 (es) | Enzimas con actividad beta-(1,6)-endoglucanasa. |