FR2670502B1 - Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee. - Google Patents

Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.

Info

Publication number
FR2670502B1
FR2670502B1 FR9015602A FR9015602A FR2670502B1 FR 2670502 B1 FR2670502 B1 FR 2670502B1 FR 9015602 A FR9015602 A FR 9015602A FR 9015602 A FR9015602 A FR 9015602A FR 2670502 B1 FR2670502 B1 FR 2670502B1
Authority
FR
France
Prior art keywords
yeast
processed
multisite
genome
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR9015602A
Other languages
English (en)
Other versions
FR2670502A1 (fr
Inventor
Alain Nicolas
Bernard Kudla
Nathalie Labat
Daniel Pardo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metex Noovistago SAS
Original Assignee
Eurolysine SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eurolysine SA filed Critical Eurolysine SA
Priority to FR9015602A priority Critical patent/FR2670502B1/fr
Priority to PCT/FR1991/001008 priority patent/WO1992010577A1/fr
Priority to CA 2098304 priority patent/CA2098304A1/fr
Priority to JP4502908A priority patent/JPH06504201A/ja
Publication of FR2670502A1 publication Critical patent/FR2670502A1/fr
Priority to EP19920902855 priority patent/EP0575338A1/fr
Application granted granted Critical
Publication of FR2670502B1 publication Critical patent/FR2670502B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR9015602A 1990-12-13 1990-12-13 Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee. Expired - Lifetime FR2670502B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR9015602A FR2670502B1 (fr) 1990-12-13 1990-12-13 Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
PCT/FR1991/001008 WO1992010577A1 (fr) 1990-12-13 1991-12-13 Cassette d'integration 'multisite' pour le genome d'une levure
CA 2098304 CA2098304A1 (fr) 1990-12-13 1991-12-13 Cassette d'integration "multisite" pour le genome d'une levure
JP4502908A JPH06504201A (ja) 1990-12-13 1991-12-13 酵母ゲノム用“多部位”組込みカセット
EP19920902855 EP0575338A1 (fr) 1990-12-13 1992-06-30 Cassette d'integration "multisite" pour le genome d'une levure

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9015602A FR2670502B1 (fr) 1990-12-13 1990-12-13 Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.

Publications (2)

Publication Number Publication Date
FR2670502A1 FR2670502A1 (fr) 1992-06-19
FR2670502B1 true FR2670502B1 (fr) 1994-09-16

Family

ID=9403187

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9015602A Expired - Lifetime FR2670502B1 (fr) 1990-12-13 1990-12-13 Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.

Country Status (5)

Country Link
EP (1) EP0575338A1 (fr)
JP (1) JPH06504201A (fr)
CA (1) CA2098304A1 (fr)
FR (1) FR2670502B1 (fr)
WO (1) WO1992010577A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7714116B2 (en) 1995-06-07 2010-05-11 Life Technologies Corporation Recombinational cloning using nucleic acids having recombination sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0587791B1 (fr) * 1991-05-31 2004-04-07 Genentech, Inc. Amelioration de l'expression par ciblage genique dans des sequences endogenes similaires a un retrovirus
WO1993024642A1 (fr) * 1992-06-04 1993-12-09 Exemplar Corporation Insertion d'adn heterologue en dehors de genes chromosomiques connus
AU5741996A (en) * 1995-05-22 1996-12-11 Chiron Corporation Position-specific integration of vector constructs into eukaryotic genomes mediated by a chimeric integrase protein
US5989914A (en) * 1996-06-03 1999-11-23 Universite Laval Integration cassette for improvement of transgenesis in eukaryotes
US6436643B1 (en) 1997-12-22 2002-08-20 Unilever Patent Holdings Bv Process for site-directed integration of multiple copies of a gene in a mould
NZ529476A (en) * 1999-10-25 2005-03-24 Invitrogen Corp Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
KR20140109183A (ko) 2013-03-05 2014-09-15 삼성전자주식회사 다중 유전자 삽입 기술을 이용한 유전자 재조합 균주 제작 방법
CN114317303A (zh) * 2021-12-17 2022-04-12 广东海纳川生物科技股份有限公司 一种分泌表达重组蝽防御素tha的重组毕赤酵母及其构建和表达方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8171087A (en) * 1986-10-27 1988-05-25 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
EP0445227A4 (en) * 1988-11-23 1991-12-27 The Regents Of The University Of California Position-specific insertion vectors and method of using same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7714116B2 (en) 1995-06-07 2010-05-11 Life Technologies Corporation Recombinational cloning using nucleic acids having recombination sites
US9309520B2 (en) 2000-08-21 2016-04-12 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

Also Published As

Publication number Publication date
FR2670502A1 (fr) 1992-06-19
JPH06504201A (ja) 1994-05-19
WO1992010577A1 (fr) 1992-06-25
EP0575338A1 (fr) 1993-12-29
CA2098304A1 (fr) 1992-06-14

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