JPH06504201A - 酵母ゲノム用“多部位”組込みカセット - Google Patents

酵母ゲノム用“多部位”組込みカセット

Info

Publication number
JPH06504201A
JPH06504201A JP4502908A JP50290892A JPH06504201A JP H06504201 A JPH06504201 A JP H06504201A JP 4502908 A JP4502908 A JP 4502908A JP 50290892 A JP50290892 A JP 50290892A JP H06504201 A JPH06504201 A JP H06504201A
Authority
JP
Japan
Prior art keywords
sequence
yeast
sigma
interest
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4502908A
Other languages
English (en)
Japanese (ja)
Inventor
ニコラ，アラン
キュドラ，ベルナール
ラバ，ナタリー
パルド，ダニエル
Original Assignee
ウーロリジンヌ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ウーロリジンヌ filed Critical ウーロリジンヌ
Publication of JPH06504201A publication Critical patent/JPH06504201A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP4502908A 1990-12-13 1991-12-13 酵母ゲノム用“多部位”組込みカセット Pending JPH06504201A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9015602A FR2670502B1 (fr) 1990-12-13 1990-12-13 Cassette d'integration "multisite" dans le genome d'une levure, levure transformee et procede de preparation d'un produit d'interet par une levure ainsi transformee.
FR90/15602 1990-12-13
PCT/FR1991/001008 WO1992010577A1 (fr) 1990-12-13 1991-12-13 Cassette d'integration 'multisite' pour le genome d'une levure

Publications (1)

Publication Number Publication Date
JPH06504201A true JPH06504201A (ja) 1994-05-19

Family

ID=9403187

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4502908A Pending JPH06504201A (ja) 1990-12-13 1991-12-13 酵母ゲノム用“多部位”組込みカセット

Country Status (5)

Country Link
EP (1) EP0575338A1 (fr)
JP (1) JPH06504201A (fr)
CA (1) CA2098304A1 (fr)
FR (1) FR2670502B1 (fr)
WO (1) WO1992010577A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0587791B1 (fr) * 1991-05-31 2004-04-07 Genentech, Inc. Amelioration de l'expression par ciblage genique dans des sequences endogenes similaires a un retrovirus
WO1993024642A1 (fr) * 1992-06-04 1993-12-09 Exemplar Corporation Insertion d'adn heterologue en dehors de genes chromosomiques connus
AU5741996A (en) * 1995-05-22 1996-12-11 Chiron Corporation Position-specific integration of vector constructs into eukaryotic genomes mediated by a chimeric integrase protein
US5989914A (en) * 1996-06-03 1999-11-23 Universite Laval Integration cassette for improvement of transgenesis in eukaryotes
ATE341621T1 (de) 1997-10-24 2006-10-15 Invitrogen Corp Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US6436643B1 (en) 1997-12-22 2002-08-20 Unilever Patent Holdings Bv Process for site-directed integration of multiple copies of a gene in a mould
NZ529476A (en) * 1999-10-25 2005-03-24 Invitrogen Corp Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
KR20140109183A (ko) 2013-03-05 2014-09-15 삼성전자주식회사 다중 유전자 삽입 기술을 이용한 유전자 재조합 균주 제작 방법
CN114317303A (zh) * 2021-12-17 2022-04-12 广东海纳川生物科技股份有限公司 一种分泌表达重组蝽防御素tha的重组毕赤酵母及其构建和表达方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8171087A (en) * 1986-10-27 1988-05-25 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
EP0445227A4 (en) * 1988-11-23 1991-12-27 The Regents Of The University Of California Position-specific insertion vectors and method of using same

Also Published As

Publication number Publication date
FR2670502A1 (fr) 1992-06-19
WO1992010577A1 (fr) 1992-06-25
EP0575338A1 (fr) 1993-12-29
CA2098304A1 (fr) 1992-06-14
FR2670502B1 (fr) 1994-09-16

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