FI105695B - Analoger av subtilisin, förfarande för framställning av dessa, deras användning och DNA-sekvens som kodar för dessa - Google Patents
Analoger av subtilisin, förfarande för framställning av dessa, deras användning och DNA-sekvens som kodar för dessa Download PDFInfo
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- FI105695B FI105695B FI885719A FI885719A FI105695B FI 105695 B FI105695 B FI 105695B FI 885719 A FI885719 A FI 885719A FI 885719 A FI885719 A FI 885719A FI 105695 B FI105695 B FI 105695B
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- subtilisin
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- General Chemical & Material Sciences (AREA)
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- Enzymes And Modification Thereof (AREA)
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Claims (22)
1.- Subtilisinanalog, kännetecknad av att den har en aminosyrasekvens av det i naturen förekommande 5 Bacillus-subtilisinet, vilken modifierats genom att: (1) ersätta en eller flera av aminosyrorna Asp41, Leu75, Asn7*, Asn77, Ser7B, Ile79, Gly80, Val81, Thr208 och Tyr214, vilka är närvarande vid ett kalciumbindningsställe av det i naturen förekommande Bacillus-subtilisinet, med ίο aminosyran Asp eller Glu; och (2) avlägsna eller ersätta den ena eller bägge Asn- och Gly-aminosyrorna i vilken som heist Asn-Gly- sekvens av det i _naturen förekommande Bacillus- subtilisinet med en oiik,neutral aiifatisk aminosyra.
2. Subtilisinanalog enligt patentkrav 1, kän netecknad av att __analogen är en analog av ett i naturen förekommande_____Bacillus-subtilisin, vilken valts frän en grupp bildad av subtilisin Carlsberg, subtilisin DY, subtilisin BPN' och ett aprA-subtilisin av Bacillus 20 subtilis. _________
3. Subtilisinanalog enligt patentkrav 1, kän netecknad av att Asn76 ersatts med Asp76.
4. Subtilisinanalog enligt patentkrav 1, kännetecknad av att Asn77 ersatts med Asp77.
5. Subtilisinanalog enligt patentkrav 1, kän netecknad av att Ile79 ersatts med Glu79.
6. Subtilisinanalog enligt patentkrav 1, kän netecknad av att Asn7* ersatts med Asp7* och Asn77 med Asp77.
7. Subtilisinanalog enligt patentkrav 1, kän netecknad av att Asn76 ersatts med Asp76 och Ile79 med Glu79.
8. Subtilisinanalog enligt patentkrav 1, kän netecknad av att en Asn-rest i Asn-Gly-sekvensen er- 35 satts med en rest av en olik aminosyra. • · 105695
9. Subtilisinanalog enligt patentkrav 8, kän- netecknad av att Asn-resten i Asn-Gly-sekvensen er- satts med en Ser-rest.
10. Subtilisinanalog enligt patentkrav 9, kän- 5 netecknad av att Asn-resten i ställning 109 ersatts med en Ser-rest.
11. Subtilisinanalog enligt patentkrav 9, kän- netecknad av att Asn-resten i ställning 218 ersatts med en Ser-rest. io
12. Subtilisinanalog enligt patentkrav 9, kän- netecknad av att Asn-resten i ställningarna 109 och 218 ersatts med en Ser-rest.
13. Subtilisinanalog enligt patentkrav 12, kän-netecknad av att den valts frän en grupp bildad av 15 [Asp76, Ser109, Ser218]-subtilisin, [Asp77, Ser109, Ser218]- subtilisin, [Glu79, Ser109, Ser218]-subtil isin, [Asp76, Asp77, Ser109, Ser218]-subtilisin och [Asp76, Glu79, Ser109, Ser218]- subtilisin.
14. Subtilisinanalog enligt patentkrav 1, kän - 20 netecknad av att det modifierbara Bacillus-sub- tilisinet har en i naturen förekommande aminosyrasekvens som visas i tabell 1.
15. Subtilisinanalog enligt patentkrav 14, [Asp76, Ser109, Ser218]-subtilisin.
16. Subtilisinanalog enligt patentkrav 14, [Asp77, Ser109, Ser218]-subtilisin.
17. Subtilisinanalog enligt patentkrav 14, [Glu79, Ser109, Ser218] - subtil isin.
18. Subtilisinanalog enligt patentkrav 14, [Asp76,
30 Asp77, Ser109, Ser218]-subtilisin.
19. Subtilisinanalog enligt patentkrav 14, [Asp76, Glu79, Ser109, Ser218]-subtilisin.
20. DNA-sekvens, kännetecknad av att den kodar en subtilisinanalog med en aminosyrasekvens av det i 35 naturen förekommande Bacillus-subtilisinet, vilken sekvens modifierats genom att: • · 51 105695 (1) ersätta en eller flera av aminosyrorna Asp41, Leu75, Asn76, Asn77, Ser78, Ile79, Gly80, Vai81, Thr208 och Tyr214, vilka är närvarande vid ett kalciumbindningsställe av det i naturen förekommande Bacillus-subtilisinet, med 5 aminosyran Asp eller Glu; och (2) avlägsna eller ersätta den ena eller bägge Asn- och Gly-aminosyrorna i vilken som heist Asn-Gly-sekvens av det i naturen förekommande Bacillus-sub-tilisinet med en oiik neutral alifatisk aminosyra. ίο
21. Förfarande för att framställa Bacillus-sub- tilisin med förbättrad värme- och pH-stabilitet och omf attande ett kalciumbindningsställe, kännetecknat av att en DNA-sekvens enligt patentkrav 20 införs i en bakterie-värdcell, där den bringas att expressera och 15 nämnda Bacillus-subtilisin tillvaratas.
22. Användning av subtilisinanalog enligt patentkrav 1 i en tvättmedelssammansättning. * • «
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3687287 | 1987-04-10 | ||
US07/036,872 US4914031A (en) | 1987-04-10 | 1987-04-10 | Subtilisin analogs |
PCT/US1988/001038 WO1988008033A1 (en) | 1987-04-10 | 1988-03-28 | Subtilisin analogs |
US8801038 | 1988-03-28 |
Publications (3)
Publication Number | Publication Date |
---|---|
FI885719A FI885719A (fi) | 1988-12-09 |
FI885719A0 FI885719A0 (fi) | 1988-12-09 |
FI105695B true FI105695B (sv) | 2000-09-29 |
Family
ID=21891131
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI885719A FI105695B (sv) | 1987-04-10 | 1988-12-09 | Analoger av subtilisin, förfarande för framställning av dessa, deras användning och DNA-sekvens som kodar för dessa |
Country Status (15)
Country | Link |
---|---|
US (1) | US4914031A (sv) |
EP (1) | EP0309565B2 (sv) |
JP (1) | JP2590247B2 (sv) |
KR (1) | KR960006120B1 (sv) |
AT (1) | ATE119941T1 (sv) |
AU (1) | AU612653B2 (sv) |
CA (1) | CA1316473C (sv) |
DE (1) | DE3853328T3 (sv) |
DK (1) | DK175576B1 (sv) |
FI (1) | FI105695B (sv) |
HK (1) | HK1007168A1 (sv) |
IL (1) | IL85953A (sv) |
LV (1) | LV11043B (sv) |
NO (1) | NO179679C (sv) |
WO (1) | WO1988008033A1 (sv) |
Families Citing this family (110)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5185258A (en) * | 1984-05-29 | 1993-02-09 | Genencor International, Inc. | Subtilisin mutants |
EP0254735B2 (en) * | 1986-01-15 | 1998-06-17 | Amgen Inc. | METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS |
DE3750916T2 (de) * | 1986-04-30 | 1995-06-01 | Genencor Int | Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte. |
US5260207A (en) * | 1987-04-06 | 1993-11-09 | Enzon Labs Inc. | Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
JP3155984B2 (ja) * | 1987-04-06 | 2001-04-16 | ノヴォザイムズ・アクティーゼルスカブ | タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作 |
DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
CN1056187C (zh) * | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
US6287841B1 (en) | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
US5324653A (en) * | 1988-02-11 | 1994-06-28 | Gist-Brocades N.V. | Recombinant genetic means for the production of serine protease muteins |
US5246849A (en) * | 1988-04-12 | 1993-09-21 | Enzon, Inc. | Thermally stable serine proteases |
US5116741A (en) * | 1988-04-12 | 1992-05-26 | Genex Corporation | Biosynthetic uses of thermostable proteases |
US5122449A (en) * | 1988-10-07 | 1992-06-16 | Eastman Kodak Company | Use of a protease in the extraction of chlamydial, gonococcal and herpes antigens |
AU618675B2 (en) * | 1989-05-17 | 1992-01-02 | Amgen, Inc. | Multiply mutated subtilisins |
US5665587A (en) | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
DK316989D0 (da) * | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
DE69034159T2 (de) * | 1989-06-26 | 2005-02-17 | Unilever N.V. | Enzymatische Waschmittelzusammensetzungen |
US5658871A (en) * | 1989-07-07 | 1997-08-19 | Lever Brothers Company, Division Of Conopco, Inc. | Microbial lipase muteins and detergent compositions comprising same |
ES2144990T3 (es) * | 1989-08-25 | 2000-07-01 | Henkel Of America Inc | Enzima proteolitica alcalina y metodo de produccion. |
US6271012B1 (en) * | 1989-10-11 | 2001-08-07 | Genencor International, Inc. | Protease muteins and their use in detergents |
US5541062A (en) * | 1990-02-23 | 1996-07-30 | Arch Development Corporation | Methods and compositions for preparing protein processing enzymes |
IT1239733B (it) * | 1990-02-23 | 1993-11-15 | Eniricerche Spa | Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione |
DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
EP0995801A1 (de) * | 1991-07-27 | 2000-04-26 | Genencor International GmbH | Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme |
US5399484A (en) * | 1991-10-08 | 1995-03-21 | Eastman Kodak Company | Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor |
US5470733A (en) * | 1993-06-01 | 1995-11-28 | University Of Maryland | Calcium free subtilisin mutants |
US5567601A (en) * | 1993-06-01 | 1996-10-22 | University Of Maryland | Subtilisin mutants lacking a primary calcium binding site |
US6440717B1 (en) | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
ATE361355T1 (de) * | 1993-10-14 | 2007-05-15 | Procter & Gamble | Proteasehaltige reinigungsmittel |
MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
CA2173106C (en) * | 1993-10-14 | 2000-02-15 | Michael Eugene Burns | Bleaching compositions comprising protease enzymes |
ATE193319T1 (de) * | 1993-12-03 | 2000-06-15 | Buckman Labor Inc | Enzymstabilisierung durch blockcopolymere |
US6599730B1 (en) | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
US6455295B1 (en) | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
BR9712878A (pt) | 1996-11-04 | 2000-02-01 | Novo Nordisk As | Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase. |
BR9712473B1 (pt) | 1996-11-04 | 2009-08-11 | variantes de subtilase e composições. | |
ATE385254T1 (de) | 1997-08-29 | 2008-02-15 | Novozymes As | Proteasevarianten und zusammensetzungen |
CN1272137A (zh) | 1997-08-29 | 2000-11-01 | 诺沃挪第克公司 | 蛋白酶变体及组合物 |
ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
US6780629B2 (en) | 1997-11-21 | 2004-08-24 | Novozymes A/S | Subtilase enzymes |
ATE298791T1 (de) | 1997-11-21 | 2005-07-15 | Novozymes As | Protease-varianten und zusammensetzungen |
US6773907B2 (en) | 1997-11-21 | 2004-08-10 | Peter Kamp Hansen | Subtilase enzymes |
US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
US6495136B1 (en) | 1998-03-26 | 2002-12-17 | The Procter & Gamble Company | Proteases having modified amino acid sequences conjugated to addition moieties |
BR9909130A (pt) * | 1998-03-26 | 2000-12-19 | Procter & Gamble | Variantes de protease de serina tendo substituições de amino-ácido |
US6642011B2 (en) | 1998-04-15 | 2003-11-04 | Genencor International, Inc. | Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins |
US6831053B1 (en) | 1998-10-23 | 2004-12-14 | The Procter & Gamble Company | Bleaching compositions comprising multiply-substituted protease variants |
US6376450B1 (en) * | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
DE69943346D1 (de) | 1998-12-18 | 2011-05-19 | Novozymes As | Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion |
AU4393000A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 128 and 129 |
ATE402996T1 (de) | 1999-05-20 | 2008-08-15 | Novozymes As | Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126 |
WO2000071689A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128 |
DE60040284D1 (de) | 1999-05-20 | 2008-10-30 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130 |
AU4392800A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127 |
AU4392500A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 132 and 133 |
CN1373802A (zh) * | 1999-07-22 | 2002-10-09 | 宝洁公司 | 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体 |
BR0012660A (pt) | 1999-07-22 | 2002-04-09 | Procter & Gamble | Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal |
CZ2002171A3 (cs) | 1999-07-22 | 2002-06-12 | The Procter & Gamble Company | Proteinázový konjugát, čistící prostředek a prostředek osobní péče |
US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
US6727085B2 (en) * | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
US6777218B1 (en) | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
AU2001280968A1 (en) * | 2000-07-31 | 2002-02-13 | Menzel, Rolf | Compositions and methods for directed gene assembly |
US6541234B1 (en) * | 2000-09-11 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
US6541235B1 (en) * | 2000-09-29 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
ES2309167T3 (es) | 2001-02-19 | 2008-12-16 | Merck Patent Gmbh | Metodo para identificar epitotes de celulas t y metodo para preparar moleculas con inmunogenicidad reducida. |
US6929939B2 (en) * | 2001-03-23 | 2005-08-16 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
DE10163884A1 (de) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
EP2287320B1 (en) * | 2002-01-16 | 2014-10-01 | Danisco US Inc. | Multiply-substituted protease variants |
AU2003228393A1 (en) | 2002-03-29 | 2003-10-13 | Genencor International, Inc. | Ehanced protein expression in bacillus |
US7294499B2 (en) * | 2003-01-30 | 2007-11-13 | Novozymes A/S | Subtilases |
CA2526341C (en) * | 2003-05-07 | 2013-02-19 | Novozymes A/S | Variant subtilisin enzymes (subtilases) |
ATE516347T1 (de) | 2003-10-23 | 2011-07-15 | Novozymes As | Protease mit verbesserter stabilität in detergentien |
DE10360805A1 (de) | 2003-12-23 | 2005-07-28 | Henkel Kgaa | Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease |
WO2005078074A2 (en) * | 2004-02-13 | 2005-08-25 | Novozymes A/S | Protease variants |
EP1735339A2 (en) * | 2004-04-09 | 2006-12-27 | Genencor International, Inc. | Pcka modifications and enhanced protein expression in bacillus |
DE102004019751A1 (de) | 2004-04-23 | 2005-11-17 | Henkel Kgaa | Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen |
KR100750659B1 (ko) * | 2005-02-18 | 2007-08-20 | 인하대학교 산학협력단 | 계면활성제 및 산화제에 매우 안정한 호염, 호알카리성 단백질 분해효소 및 이를 암호화하는 유전자 |
MX2008012999A (es) | 2006-04-14 | 2008-10-17 | Genencor Int | Tratamiento de una etapa de textiles. |
US20090215663A1 (en) | 2006-04-20 | 2009-08-27 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
US20080090745A1 (en) * | 2006-10-13 | 2008-04-17 | Fox Bryan P | Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp. |
KR20090129425A (ko) * | 2007-03-12 | 2009-12-16 | 다니스코 유에스 인크. | 개질된 프로테아제 |
JP5427776B2 (ja) | 2007-04-30 | 2014-02-26 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | メタロプロテアーゼ洗剤調合剤を安定化するためのタンパク質の加水分解物の使用 |
EP2152732B1 (en) * | 2007-05-10 | 2012-03-21 | Danisco US Inc. | A modified secretion system to increase expression of polypeptides in bacteria |
SG148934A1 (en) | 2007-06-11 | 2009-01-29 | Novozymes As | A process for combined biopolishing and bleach clean-up |
DE102007032111B4 (de) | 2007-07-09 | 2017-07-20 | Henkel Ag & Co. Kgaa | Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen |
DE102007036756A1 (de) | 2007-08-03 | 2009-02-05 | Henkel Ag & Co. Kgaa | Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen |
DE102007051092A1 (de) * | 2007-10-24 | 2009-04-30 | Henkel Ag & Co. Kgaa | Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin |
CN101903519B (zh) | 2007-12-21 | 2013-07-31 | 丹尼斯科美国公司 | 杆菌中增强的蛋白质生产 |
JP5659143B2 (ja) * | 2008-03-28 | 2015-01-28 | ダニスコ・ユーエス・インク | 細菌性細胞中の遺伝子座を増幅する方法 |
AU2009256169A1 (en) * | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Compositions and methods comprising variant microbial proteases |
AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
CN103154263A (zh) | 2010-08-19 | 2013-06-12 | 诺维信公司 | 诱导的孢子形成筛选方法 |
MX2014006205A (es) * | 2011-11-25 | 2014-07-14 | Novozymes As | Variantes de subtilasa y polinucleotidos que las codifican. |
US10287591B2 (en) | 2013-12-31 | 2019-05-14 | Danisco Us Inc | Enhanced protein expression |
EP3164486B1 (en) | 2014-07-04 | 2020-05-13 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3233893B1 (en) | 2014-12-16 | 2021-01-20 | Danisco US Inc. | Enhanced protein expression |
JP2017538433A (ja) | 2014-12-19 | 2017-12-28 | ダニスコ・ユーエス・インク | タンパク質の発現増大 |
WO2018156705A1 (en) | 2017-02-24 | 2018-08-30 | Danisco Us Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
US11879127B2 (en) | 2017-08-23 | 2024-01-23 | Danisco Us Inc. | Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains |
KR20200047668A (ko) | 2017-09-13 | 2020-05-07 | 다니스코 유에스 인크. | 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열 |
EP3703661A1 (en) | 2017-11-02 | 2020-09-09 | Danisco US Inc. | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
KR20200105879A (ko) | 2018-01-03 | 2020-09-09 | 다니스코 유에스 인크. | 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법 |
WO2020099491A1 (en) | 2018-11-14 | 2020-05-22 | Novozymes A/S | Oral care composition comprising a polypeptide having dnase activity |
EP4090738A1 (en) | 2020-01-15 | 2022-11-23 | Danisco US Inc. | Compositions and methods for enhanced protein production in bacillus licheniformis |
US20230332124A1 (en) | 2020-08-24 | 2023-10-19 | Novozymes A/S | Oral care composition comprising a fructanase |
CN117881772A (zh) | 2021-08-20 | 2024-04-12 | 丹尼斯科美国公司 | 编码新型核酸酶的多核苷酸、其组合物及其从蛋白质制剂中消除dna的方法 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1240058A (en) * | 1968-04-12 | 1971-07-21 | Procter & Gamble | Enzyme-containing detergent compositions |
US3623957A (en) * | 1970-01-21 | 1971-11-30 | Baxter Laboratories Inc | Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis |
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
JPS60160884A (ja) * | 1984-02-01 | 1985-08-22 | Showa Denko Kk | 酵素安定化法 |
EP0199405B1 (en) * | 1985-04-15 | 1992-06-24 | The Procter & Gamble Company | Liquid detergents containing surfactant, proteolytic enzyme and boric acid |
EP0260299A4 (en) * | 1986-02-12 | 1988-11-24 | Genex Corp | MUTAGENESIS AND SCREENING PROCESS AND PRODUCT OBTAINED. |
DE3750916T2 (de) * | 1986-04-30 | 1995-06-01 | Genencor Int | Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte. |
JPH0657151B2 (ja) * | 1986-05-15 | 1994-08-03 | 昭和電工株式会社 | 酵素の安定化法 |
AU620326B2 (en) * | 1987-02-27 | 1992-02-20 | Dsm Ip Assets B.V. | Stable gene amplification in chromosomal dna of prokaryotic microorganisms |
JP3155984B2 (ja) * | 1987-04-06 | 2001-04-16 | ノヴォザイムズ・アクティーゼルスカブ | タンパク質の安定化のための金属イオン結合部位における静電的相互作用の操作 |
-
1987
- 1987-04-10 US US07/036,872 patent/US4914031A/en not_active Expired - Lifetime
-
1988
- 1988-03-28 AT AT88904041T patent/ATE119941T1/de not_active IP Right Cessation
- 1988-03-28 AU AU17016/88A patent/AU612653B2/en not_active Expired
- 1988-03-28 DE DE3853328T patent/DE3853328T3/de not_active Expired - Lifetime
- 1988-03-28 EP EP88904041A patent/EP0309565B2/en not_active Expired - Lifetime
- 1988-03-28 WO PCT/US1988/001038 patent/WO1988008033A1/en active IP Right Grant
- 1988-03-28 KR KR1019880701638A patent/KR960006120B1/ko not_active IP Right Cessation
- 1988-03-28 JP JP63503622A patent/JP2590247B2/ja not_active Expired - Lifetime
- 1988-03-31 CA CA000563171A patent/CA1316473C/en not_active Expired - Lifetime
- 1988-03-31 IL IL85953A patent/IL85953A/xx not_active IP Right Cessation
- 1988-12-09 DK DK198806867A patent/DK175576B1/da not_active IP Right Cessation
- 1988-12-09 FI FI885719A patent/FI105695B/sv not_active IP Right Cessation
- 1988-12-09 NO NO885484A patent/NO179679C/no not_active IP Right Cessation
-
1995
- 1995-06-21 LV LVP-95-188A patent/LV11043B/en unknown
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1998
- 1998-06-23 HK HK98106059A patent/HK1007168A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
US4914031A (en) | 1990-04-03 |
AU612653B2 (en) | 1991-07-18 |
KR960006120B1 (ko) | 1996-05-09 |
DK686788D0 (da) | 1988-12-09 |
WO1988008033A1 (en) | 1988-10-20 |
DK686788A (da) | 1988-12-09 |
EP0309565B1 (en) | 1995-03-15 |
DE3853328T3 (de) | 2002-06-06 |
IL85953A (en) | 1993-01-31 |
JPH01502957A (ja) | 1989-10-12 |
NO885484D0 (no) | 1988-12-09 |
LV11043B (en) | 1996-06-20 |
EP0309565A1 (en) | 1989-04-05 |
JP2590247B2 (ja) | 1997-03-12 |
FI885719A (fi) | 1988-12-09 |
NO179679B (no) | 1996-08-19 |
DE3853328T2 (de) | 1995-07-27 |
NO179679C (no) | 1996-11-27 |
CA1316473C (en) | 1993-04-20 |
LV11043A (lv) | 1996-02-20 |
DK175576B1 (da) | 2004-12-13 |
ATE119941T1 (de) | 1995-04-15 |
EP0309565A4 (sv) | 1990-02-05 |
KR890700668A (ko) | 1989-04-26 |
EP0309565B2 (en) | 2001-08-22 |
NO885484L (no) | 1989-02-09 |
HK1007168A1 (en) | 1999-04-01 |
DE3853328D1 (de) | 1995-04-20 |
AU1701688A (en) | 1988-11-04 |
FI885719A0 (fi) | 1988-12-09 |
IL85953A0 (en) | 1988-09-30 |
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Owner name: AMGEN INC. |
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