EP0260299A4 - Mutagenesis and screening method and product. - Google Patents

Mutagenesis and screening method and product.

Info

Publication number
EP0260299A4
EP0260299A4 EP19870901901 EP87901901A EP0260299A4 EP 0260299 A4 EP0260299 A4 EP 0260299A4 EP 19870901901 EP19870901901 EP 19870901901 EP 87901901 A EP87901901 A EP 87901901A EP 0260299 A4 EP0260299 A4 EP 0260299A4
Authority
EP
European Patent Office
Prior art keywords
mutagenesis
product
screening method
screening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP19870901901
Other languages
German (de)
French (fr)
Other versions
EP0260299A1 (en
Inventor
Philip N Bryan
Michele L Rollence
Michael W Pantoliano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzon Labs Inc
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of EP0260299A1 publication Critical patent/EP0260299A1/en
Publication of EP0260299A4 publication Critical patent/EP0260299A4/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19870901901 1986-02-12 1987-02-12 Mutagenesis and screening method and product. Ceased EP0260299A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
US828545 1986-02-12

Publications (2)

Publication Number Publication Date
EP0260299A1 EP0260299A1 (en) 1988-03-23
EP0260299A4 true EP0260299A4 (en) 1988-11-24

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19870901901 Ceased EP0260299A4 (en) 1986-02-12 1987-02-12 Mutagenesis and screening method and product.

Country Status (4)

Country Link
EP (1) EP0260299A4 (en)
JP (1) JPS63502959A (en)
DK (1) DK531887A (en)
WO (1) WO1987005050A1 (en)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
JP3155984B2 (en) * 1987-04-06 2001-04-16 ノヴォザイムズ・アクティーゼルスカブ Manipulating electrostatic interactions at metal ion binding sites for protein stabilization
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
CN1056187C (en) * 1988-02-11 2000-09-06 金克克国际有限公司 Proteolytic enzymes and their use in detergents
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (en) * 1989-06-26 1989-06-26 Novo Nordisk As ENZYMES
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
EP0583339B1 (en) * 1991-05-01 1998-07-08 Novo Nordisk A/S Stabilized enzymes and detergent compositions
DE69212390T2 (en) * 1991-05-01 1997-01-09 Unilever Nv Detergent compositions containing stabilized enzymes
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
JP4044143B2 (en) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ Subtilase variants and compositions
KR100591553B1 (en) * 1996-11-04 2006-06-19 노보자임스 에이/에스 Subtilase variants and composition
CN1148444C (en) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 Protease variants and compositions
KR20010023468A (en) 1997-08-29 2001-03-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 Protease variants and compositions
DE19743683A1 (en) * 1997-10-02 1999-04-08 Basf Ag Procedure for changing the substrate specificity of enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
CA2310454C (en) 1997-11-21 2012-01-24 Novo Nordisk A/S Protease variants and compositions
CN1301305A (en) 1998-03-26 2001-06-27 宝洁公司 Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
EP1803817B1 (en) 1998-12-18 2011-04-06 Novozymes A/S Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region
DE60040285D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN ITEM 127 AND 128
WO2000071690A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
EP1183340B1 (en) 1999-05-20 2008-09-03 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
WO2000071687A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
DE60040282D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 132 AND 133
CZ2002212A3 (en) 1999-07-22 2002-07-17 The Procter & Gamble Company Variants of subtilisin protease having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CZ2002220A3 (en) 1999-07-22 2002-05-15 The Procter & Gamble Company Subtilisin protease variants with deletions and substitution of amino acids in defined epitope regions
MXPA02000842A (en) 1999-07-22 2002-07-30 Procter & Gamble Protease conjugates having sterically protected clip sites.
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (en) * 2003-04-10 2004-11-04 Kao Corp Alkali protease
CN102766545B (en) 2003-05-07 2014-08-06 诺维信公司 Variant subtilisin enzymes (subtilases)
CN102994486A (en) 2003-10-23 2013-03-27 诺维信公司 Protease with improved stability in detergents
JP4787571B2 (en) * 2004-10-08 2011-10-05 花王株式会社 Alkaline protease
MX2008012999A (en) 2006-04-14 2008-10-17 Genencor Int One-step treatment of textiles.
JP2009534022A (en) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ A sabinase variant with improved detergency against egg stains
JP5427776B2 (en) 2007-04-30 2014-02-26 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン Use of protein hydrolysates to stabilize metalloprotease detergent formulations
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
EP2606146A1 (en) 2010-08-19 2013-06-26 Novozymes A/S Induced sporulation screening method
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
CN116322625A (en) 2020-08-24 2023-06-23 诺维信公司 Oral care compositions comprising levanase

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (en) * 1978-04-18 1979-11-16 Pasteur Institut Sorting of microorganisms, esp. bacteria - by lysis of individuals in colonies and detection of specific intracellular components
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (en) * 1978-04-18 1979-11-16 Pasteur Institut Sorting of microorganisms, esp. bacteria - by lysis of individuals in colonies and detection of specific intracellular components
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GENETIC TECHNOLOGY NEWS, vol. 6, no. 5, May 1986, pages 3-4; "Genex engineers detergent enzymes" *
J. CELL. BIOCHEM., SUPPL., no. 10, part A, 1986, page 271, no. E101; P. BRYAN et al.: "Protein engineering of subtilisin: proteases of enhanced stability", & SYMPOSIUM ON PROTEASES IN BIOLOGICAL CONTROL AND BIOTECHNOLOGY, 15TH ANNUAL UCLA, Los Angeles, 9th-15th February 1986 *
PROC. NATL. ACAD. SCI. USA, vol. 82, February 1985, pages 747-750; T. ALBER et al.: "A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme" *
PROTEINS: STRUCTURE, FUNCTION AND GENETICS, vol. 1, 10th November 1986, pages 326-334; P.N. BRYAN et al.: "Proteases of enhanced stability: characterization of a thermostable variant of subtilisin" *
SCIENCE, vol. 229, no. 4719, 20th September 1985, pages 1193-1201; D. BOTSTEIN et al.: "Strategies and applications of in vitro mutagenesis" *
See also references of WO8705050A1 *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 05 December 1985, The American Society of Biological Chemists Inc., US; M. MATSUMURA et al., pp. 15298-15303# *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 5th December 1985, pages 15298-15303, The American Society of Biological Chemists, Inc., US; M. MATSUMURA et al.: "Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus" *

Also Published As

Publication number Publication date
WO1987005050A1 (en) 1987-08-27
DK531887D0 (en) 1987-10-12
DK531887A (en) 1987-10-12
EP0260299A1 (en) 1988-03-23
JPS63502959A (en) 1988-11-02

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 19910802

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RIN1 Information on inventor provided before grant (corrected)

Inventor name: PANTOLIANO, MICHAEL, W.

Inventor name: BRYAN, PHILIP, N.

Inventor name: ROLLENCE, MICHELE, L.