EP0260299A4 - Procede de mutagenese et de criblage et produit obtenu. - Google Patents

Procede de mutagenese et de criblage et produit obtenu.

Info

Publication number
EP0260299A4
EP0260299A4 EP19870901901 EP87901901A EP0260299A4 EP 0260299 A4 EP0260299 A4 EP 0260299A4 EP 19870901901 EP19870901901 EP 19870901901 EP 87901901 A EP87901901 A EP 87901901A EP 0260299 A4 EP0260299 A4 EP 0260299A4
Authority
EP
European Patent Office
Prior art keywords
mutagenesis
product
screening method
screening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP19870901901
Other languages
German (de)
English (en)
Other versions
EP0260299A1 (fr
Inventor
Philip N Bryan
Michele L Rollence
Michael W Pantoliano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzon Labs Inc
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of EP0260299A1 publication Critical patent/EP0260299A1/fr
Publication of EP0260299A4 publication Critical patent/EP0260299A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19870901901 1986-02-12 1987-02-12 Procede de mutagenese et de criblage et produit obtenu. Ceased EP0260299A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
US828545 1986-02-12

Publications (2)

Publication Number Publication Date
EP0260299A1 EP0260299A1 (fr) 1988-03-23
EP0260299A4 true EP0260299A4 (fr) 1988-11-24

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19870901901 Ceased EP0260299A4 (fr) 1986-02-12 1987-02-12 Procede de mutagenese et de criblage et produit obtenu.

Country Status (4)

Country Link
EP (1) EP0260299A4 (fr)
JP (1) JPS63502959A (fr)
DK (1) DK531887D0 (fr)
WO (1) WO1987005050A1 (fr)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3856593D1 (de) * 1987-04-06 2007-10-04 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
ES2090481T3 (es) * 1991-05-01 1996-10-16 Unilever Nv Composiciones detergentes que contienen enzimas estabilizadas.
JP3471797B2 (ja) * 1991-05-01 2003-12-02 ノボザイムス アクティーゼルスカブ 安定化酵素及び洗剤
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
JP2001503269A (ja) * 1996-11-04 2001-03-13 ノボ ノルディスク アクティーゼルスカブ ズブチラーゼ変異体及び、組成物
EP2278001B1 (fr) 1996-11-04 2013-10-23 Novozymes A/S Variants et compositions de protéase
JP5095884B2 (ja) 1997-08-29 2012-12-12 ノボザイムス アクティーゼルスカブ プロテアーゼ変異体及び組成物
WO1999011769A1 (fr) 1997-08-29 1999-03-11 Novo Nordisk A/S Variants de la protease et compositions
DE19743683A1 (de) * 1997-10-02 1999-04-08 Basf Ag Verfahren zur Änderung der Substratspezifität von Enzymen
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
JP4246384B2 (ja) 1997-11-21 2009-04-02 ノボザイムス アクティーゼルスカブ プロテアーゼ変異体及び組成物
BR9909130A (pt) * 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
DE69943346D1 (de) 1998-12-18 2011-05-19 Novozymes As Subtilase Enzyme der I-S1- und I-S2-Untergruppen mit einem zusätzlichen Aminosäurerest in einer aktiven Schleifenregion
ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
ATE408680T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
MXPA02000840A (es) 1999-07-22 2002-07-30 Procter & Gamble Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CZ2002220A3 (cs) 1999-07-22 2002-05-15 The Procter & Gamble Company Varianty subtilisinové proteasy s delecemi a substitucemi aminokyselin v definovaných epitopových regionech
CA2379729A1 (fr) 1999-07-22 2001-02-01 The Procter & Gamble Company Conjugues de protease comprenant des sites clip proteges steriquement
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (ja) * 2003-04-10 2004-11-04 Kao Corp アルカリプロテアーゼ
CN102766545B (zh) 2003-05-07 2014-08-06 诺维信公司 变体枯草杆菌蛋白酶（枯草杆菌酶）
CN1871344A (zh) 2003-10-23 2006-11-29 诺和酶股份有限公司 在洗涤剂中具有改善稳定性的蛋白酶
JP4787571B2 (ja) * 2004-10-08 2011-10-05 花王株式会社 アルカリプロテアーゼ
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
EP2013339B1 (fr) 2006-04-20 2013-09-11 Novozymes A/S Variants de savinase offrant une meilleure efficacité de lavage contre les taches d'oeuf
CA2685690A1 (fr) 2007-04-30 2008-11-06 Danisco Us Inc. Utilisation d'hydrolysats de proteine pour stabiliser des formulations detersives de metalloprotease
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
EP2606146A1 (fr) 2010-08-19 2013-06-26 Novozymes A/S Procédé de criblage d'une sporulation induite
WO2020099490A1 (fr) 2018-11-14 2020-05-22 Novozymes A/S Composition de soin buccal comprenant des enzymes
KR20230057391A (ko) 2020-08-24 2023-04-28 노보자임스 에이/에스 프럭타나제를 포함하는 구강 관리 조성물

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (fr) * 1978-04-18 1979-11-16 Pasteur Institut Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine
EP0108303A1 (fr) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Procédé pour la détection et l'isolation d'un micro-organisme
EP0138075A1 (fr) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Méthode pour la génération et détection de variantes d'enzymes à thermostabilité et activité élevée
WO1987004461A1 (fr) * 1986-01-15 1987-07-30 Amgen ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH, ET LEUR PROCEDE DE PRODUCTION

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (fr) * 1978-04-18 1979-11-16 Pasteur Institut Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine
EP0108303A1 (fr) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Procédé pour la détection et l'isolation d'un micro-organisme
EP0138075A1 (fr) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Méthode pour la génération et détection de variantes d'enzymes à thermostabilité et activité élevée
WO1987004461A1 (fr) * 1986-01-15 1987-07-30 Amgen ANALOGUES DE SUBTILISINE THERMIQUEMENT STABLE ET STABLE AU pH, ET LEUR PROCEDE DE PRODUCTION

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GENETIC TECHNOLOGY NEWS, vol. 6, no. 5, May 1986, pages 3-4; "Genex engineers detergent enzymes" *
J. CELL. BIOCHEM., SUPPL., no. 10, part A, 1986, page 271, no. E101; P. BRYAN et al.: "Protein engineering of subtilisin: proteases of enhanced stability", & SYMPOSIUM ON PROTEASES IN BIOLOGICAL CONTROL AND BIOTECHNOLOGY, 15TH ANNUAL UCLA, Los Angeles, 9th-15th February 1986 *
PROC. NATL. ACAD. SCI. USA, vol. 82, February 1985, pages 747-750; T. ALBER et al.: "A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme" *
PROTEINS: STRUCTURE, FUNCTION AND GENETICS, vol. 1, 10th November 1986, pages 326-334; P.N. BRYAN et al.: "Proteases of enhanced stability: characterization of a thermostable variant of subtilisin" *
SCIENCE, vol. 229, no. 4719, 20th September 1985, pages 1193-1201; D. BOTSTEIN et al.: "Strategies and applications of in vitro mutagenesis" *
See also references of WO8705050A1 *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 05 December 1985, The American Society of Biological Chemists Inc., US; M. MATSUMURA et al., pp. 15298-15303# *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 5th December 1985, pages 15298-15303, The American Society of Biological Chemists, Inc., US; M. MATSUMURA et al.: "Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus" *

Also Published As

Publication number Publication date
DK531887A (da) 1987-10-12
WO1987005050A1 (fr) 1987-08-27
EP0260299A1 (fr) 1988-03-23
JPS63502959A (ja) 1988-11-02
DK531887D0 (da) 1987-10-12

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Inventor name: PANTOLIANO, MICHAEL, W.

Inventor name: BRYAN, PHILIP, N.

Inventor name: ROLLENCE, MICHELE, L.