DK531887A - Mutagenese- og screeningsfremgangsmaade samt produkt - Google Patents

Mutagenese- og screeningsfremgangsmaade samt produkt

Info

Publication number
DK531887A
DK531887A DK531887A DK531887A DK531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A
Authority
DK
Denmark
Prior art keywords
mutagenese
product
screening procedure
screening
procedure
Prior art date
Application number
DK531887A
Other languages
English (en)
Other versions
DK531887D0 (da
Inventor
Phillip N Bryan
Michele L Rollence
Michael W Pantoliano
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of DK531887D0 publication Critical patent/DK531887D0/da
Publication of DK531887A publication Critical patent/DK531887A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK531887A 1986-02-12 1987-10-12 Mutagenese- og screeningsfremgangsmaade samt produkt DK531887A (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
PCT/US1987/000348 WO1987005050A1 (en) 1986-02-12 1987-02-12 Mutagenesis and screening method and product

Publications (2)

Publication Number Publication Date
DK531887D0 DK531887D0 (da) 1987-10-12
DK531887A true DK531887A (da) 1987-10-12

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
DK531887A DK531887A (da) 1986-02-12 1987-10-12 Mutagenese- og screeningsfremgangsmaade samt produkt

Country Status (4)

Country Link
EP (1) EP0260299A4 (da)
JP (1) JPS63502959A (da)
DK (1) DK531887A (da)
WO (1) WO1987005050A1 (da)

Families Citing this family (62)

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US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0916732B1 (en) * 1987-04-06 2007-08-22 Novozymes A/S The engineering of electronic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
EP0516200B1 (en) * 1991-05-01 1996-07-24 Unilever N.V. Detergent compositions containing stabilized enzymes
DE69226182T2 (de) * 1991-05-01 1999-01-21 Novo Nordisk As Stabilisierte enzyme und waschmittelzusammensetzungen
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
EP0948610B1 (en) 1996-11-04 2011-05-25 Novozymes A/S Subtilase variants and compositions
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
EP1009815B1 (en) 1997-08-29 2008-01-30 Novozymes A/S Protease variants and compositions
EP1012251A1 (en) 1997-08-29 2000-06-28 Novo Nordisk A/S Protease variants and compositions
DE19743683A1 (de) * 1997-10-02 1999-04-08 Basf Ag Verfahren zur Änderung der Substratspezifität von Enzymen
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
KR100762164B1 (ko) 1997-11-21 2007-10-01 노보자임스 에이/에스 프로테아제 변이체 및 조성물
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
JP2002507426A (ja) 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
WO2000071691A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
AU4392900A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128
ATE408680T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU5928100A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
CZ2002171A3 (cs) 1999-07-22 2002-06-12 The Procter & Gamble Company Proteinázový konjugát, čistící prostředek a prostředek osobní péče
MXPA02000840A (es) 1999-07-22 2002-07-30 Procter & Gamble Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas.
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (ja) 2003-04-10 2004-11-04 Kao Corp アルカリプロテアーゼ
CN1784490A (zh) 2003-05-07 2006-06-07 诺和酶股份有限公司 变体枯草杆菌蛋白酶(枯草杆菌酶)
EP1678296B1 (en) 2003-10-23 2011-07-13 Novozymes A/S Protease with improved stability in detergents
JP4787571B2 (ja) * 2004-10-08 2011-10-05 花王株式会社 アルカリプロテアーゼ
BRPI0709978A2 (pt) 2006-04-14 2011-08-02 Genencor Int tratamento em etapa única de produtos têxteis
EP2013339B1 (en) 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
CN101675160A (zh) 2007-04-30 2010-03-17 丹尼斯科美国公司 蛋白质水解产物稳定金属蛋白酶洗涤剂制剂的用途
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
US20130224757A1 (en) 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
CN116322625A (zh) 2020-08-24 2023-06-23 诺维信公司 包含果聚糖酶的口腔护理组合物

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
FR2423541A1 (fr) * 1978-04-18 1979-11-16 Pasteur Institut Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine
ATE25405T1 (de) * 1982-11-01 1987-02-15 Miles Lab Verfahren zum nachweis und zur isolation eines mikroorganismus.
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF

Also Published As

Publication number Publication date
EP0260299A4 (en) 1988-11-24
DK531887D0 (da) 1987-10-12
WO1987005050A1 (en) 1987-08-27
EP0260299A1 (en) 1988-03-23
JPS63502959A (ja) 1988-11-02

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Legal Events

Date Code Title Description
AHB Application shelved due to non-payment