DK531887A - MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT - Google Patents

MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT

Info

Publication number
DK531887A
DK531887A DK531887A DK531887A DK531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A
Authority
DK
Denmark
Prior art keywords
mutagenese
product
screening procedure
screening
procedure
Prior art date
Application number
DK531887A
Other languages
Danish (da)
Other versions
DK531887D0 (en
Inventor
Phillip N Bryan
Michele L Rollence
Michael W Pantoliano
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of DK531887D0 publication Critical patent/DK531887D0/en
Publication of DK531887A publication Critical patent/DK531887A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK531887A 1986-02-12 1987-10-12 MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT DK531887A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
PCT/US1987/000348 WO1987005050A1 (en) 1986-02-12 1987-02-12 Mutagenesis and screening method and product

Publications (2)

Publication Number Publication Date
DK531887D0 DK531887D0 (en) 1987-10-12
DK531887A true DK531887A (en) 1987-10-12

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
DK531887A DK531887A (en) 1986-02-12 1987-10-12 MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT

Country Status (4)

Country Link
EP (1) EP0260299A4 (en)
JP (1) JPS63502959A (en)
DK (1) DK531887A (en)
WO (1) WO1987005050A1 (en)

Families Citing this family (62)

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US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
ATE116365T1 (en) * 1986-04-30 1995-01-15 Genencor Int MUTANT OF A NON-HUMAN CARBONYL HYDROLASE, FOR THESE DNA SEQUENCES AND VECTORS CODING AND HOSTS TRANSFORMED BY THESE VECTORS.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ATE184316T1 (en) * 1987-04-06 1999-09-15 Novo Nordisk As REGULATION OF ELECTROSTATIC INTERACTIONS AT METAL ION BINDING SITES FOR STABILIZING PROTEINS
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
PT89702B (en) * 1988-02-11 1994-04-29 Gist Brocades Nv PROCESS FOR PREPARING NEW PROTEOLITIC ENZYMES AND DETERGENTS THAT CONTAINS THEM
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (en) * 1989-06-26 1989-06-26 Novo Nordisk As ENZYMES
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
ES2090481T3 (en) * 1991-05-01 1996-10-16 Unilever Nv DETERGENT COMPOSITIONS CONTAINING STABILIZED ENZYMES.
WO1992019729A1 (en) * 1991-05-01 1992-11-12 Novo Nordisk A/S Stabilized enzymes and detergent compositions
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
WO1998020115A1 (en) 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
JP2001514846A (en) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ Protease variants and compositions
DE69839076T2 (en) 1997-08-29 2009-01-22 Novozymes A/S PROTEASE VERSIONS AND COMPOSITIONS
DE19743683A1 (en) * 1997-10-02 1999-04-08 Basf Ag Procedure for changing the substrate specificity of enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
KR100762164B1 (en) 1997-11-21 2007-10-01 노보자임스 에이/에스 Protease variants and compositions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
BR9909130A (en) * 1998-03-26 2000-12-19 Procter & Gamble Serine protease variants having amino acid substitutions
DE69943346D1 (en) 1998-12-18 2011-05-19 Novozymes As Subtilase enzymes of the I-S1 and I-S2 subgroups with an additional amino acid residue in an active loop region
DE60040149D1 (en) 1999-05-20 2008-10-16 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 126 AND 127
ATE408678T1 (en) 1999-05-20 2008-10-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 129 AND 130
DE60039693D1 (en) 1999-05-20 2008-09-11 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 125 AND 126
EP1183342B2 (en) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
DE60040282D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 132 AND 133
ATE408679T1 (en) 1999-05-20 2008-10-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 127 AND 128
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
EP1210415A2 (en) 1999-07-22 2002-06-05 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
BR0012693A (en) 1999-07-22 2002-04-09 Procter & Gamble Variant, of subtilisin-like protease; cleaning composition; and personal care composition
KR20020021395A (en) 1999-07-22 2002-03-20 데이비드 엠 모이어 Protease conjugates having sterically protected clip sites
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (en) * 2003-04-10 2004-11-04 Kao Corp Alkali protease
ATE407202T1 (en) 2003-05-07 2008-09-15 Novozymes As ENZYME VARIANTS OF SUBTILISIN (SUBTILASES)
JP4880469B2 (en) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ Protease with improved stability in detergents
JP4787571B2 (en) * 2004-10-08 2011-10-05 花王株式会社 Alkaline protease
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
EP2013339B1 (en) 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US20100190682A1 (en) 2007-04-30 2010-07-29 Sang-Kyu Lee Use of protein hydrolysates to stabilize metalloprotease detergent formulations
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
CN103154263A (en) 2010-08-19 2013-06-12 诺维信公司 Induced sporulation screening method
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
KR20230057391A (en) 2020-08-24 2023-04-28 노보자임스 에이/에스 Oral care composition comprising fructanase

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FR2423541A1 (en) * 1978-04-18 1979-11-16 Pasteur Institut Sorting of microorganisms, esp. bacteria - by lysis of individuals in colonies and detection of specific intracellular components
EP0108303B1 (en) * 1982-11-01 1987-02-04 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
JP2599946B2 (en) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド Subtilisin analog

Also Published As

Publication number Publication date
DK531887D0 (en) 1987-10-12
JPS63502959A (en) 1988-11-02
WO1987005050A1 (en) 1987-08-27
EP0260299A1 (en) 1988-03-23
EP0260299A4 (en) 1988-11-24

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Legal Events

Date Code Title Description
AHB Application shelved due to non-payment