DK531887D0 - MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT - Google Patents

MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT

Info

Publication number
DK531887D0
DK531887D0 DK531887A DK531887A DK531887D0 DK 531887 D0 DK531887 D0 DK 531887D0 DK 531887 A DK531887 A DK 531887A DK 531887 A DK531887 A DK 531887A DK 531887 D0 DK531887 D0 DK 531887D0
Authority
DK
Denmark
Prior art keywords
mutagenese
product
screening procedure
screening
procedure
Prior art date
Application number
DK531887A
Other languages
Danish (da)
Other versions
DK531887A (en
Inventor
Phillip N Bryan
Michele L Rollence
Michael W Pantoliano
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of DK531887D0 publication Critical patent/DK531887D0/en
Publication of DK531887A publication Critical patent/DK531887A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK531887A 1986-02-12 1987-10-12 MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT DK531887A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
PCT/US1987/000348 WO1987005050A1 (en) 1986-02-12 1987-02-12 Mutagenesis and screening method and product

Publications (2)

Publication Number Publication Date
DK531887D0 true DK531887D0 (en) 1987-10-12
DK531887A DK531887A (en) 1987-10-12

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
DK531887A DK531887A (en) 1986-02-12 1987-10-12 MUTAGENESE AND SCREENING PROCEDURE AND PRODUCT

Country Status (4)

Country Link
EP (1) EP0260299A4 (en)
JP (1) JPS63502959A (en)
DK (1) DK531887A (en)
WO (1) WO1987005050A1 (en)

Families Citing this family (62)

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US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
ES2068181T3 (en) * 1986-04-30 1995-04-16 Genencor Int MUTANTS OF NON-HUMAN CARBONYL HYDROLASE, DNA SEQUENCES AND VECTORS CODING THEMSELVES AND HOSTS TRANSFORMED WITH THESE VECTORS.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3856362T2 (en) * 1987-04-06 2000-05-18 Novo Nordisk A/S, Bagsvaerd CONTROL OF ELECTROSTATIC INTERACTIONS AT METALION BINDING POINTS FOR THE STABILIZATION OF PROTEINS
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
PT89702B (en) 1988-02-11 1994-04-29 Gist Brocades Nv PROCESS FOR PREPARING NEW PROTEOLITIC ENZYMES AND DETERGENTS THAT CONTAINS THEM
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (en) * 1989-06-26 1989-06-26 Novo Nordisk As ENZYMES
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE69226182T2 (en) * 1991-05-01 1999-01-21 Novo Nordisk A/S, Bagsvaerd STABILIZED ENZYMES AND DETERGENT COMPOSITIONS
DE69212390T2 (en) * 1991-05-01 1997-01-09 Unilever Nv Detergent compositions containing stabilized enzymes
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
CN1554750B (en) 1996-11-04 2011-05-18 诺维信公司 Subtilase variants and compositions
DE69739020D1 (en) * 1996-11-04 2008-11-13 Novozymes As SUBTILASE VARIANTS AND COMPOUNDS
KR100720594B1 (en) 1997-08-29 2007-05-21 노보자임스 에이/에스 Protease variants and compositions
AU9061798A (en) 1997-08-29 1999-03-22 Novo Nordisk A/S Protease variants and compositions
DE19743683A1 (en) * 1997-10-02 1999-04-08 Basf Ag Procedure for changing the substrate specificity of enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
BR9814236A (en) 1997-11-21 2000-10-03 Novo Nordisk As Subtilase enzyme, its variant, isolated DNA sequence, expression vector, host microbial cell, process to produce a subtilase or a subtilase variant, composition, and use of a subtilase or a subtilase variant.
CN1301305A (en) 1998-03-26 2001-06-27 宝洁公司 Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
ATE504651T1 (en) 1998-12-18 2011-04-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
WO2000071689A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
EP1183339B2 (en) 1999-05-20 2013-03-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
WO2000071690A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
WO2000071685A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
BR0012694A (en) 1999-07-22 2002-04-09 Procter & Gamble Protease conjugate, cleaning composition and personal treatment composition
WO2001007575A2 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
AU6085800A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (en) * 2003-04-10 2004-11-04 Kao Corp Alkali protease
ATE407202T1 (en) 2003-05-07 2008-09-15 Novozymes As ENZYME VARIANTS OF SUBTILISIN (SUBTILASES)
CN1871344A (en) 2003-10-23 2006-11-29 诺和酶股份有限公司 Protease with improved stability in detergents
JP4787571B2 (en) * 2004-10-08 2011-10-05 花王株式会社 Alkaline protease
BRPI0709978A2 (en) 2006-04-14 2011-08-02 Genencor Int one-step treatment of textile products
CN101460618A (en) 2006-04-20 2009-06-17 诺维信公司 Savinase variants having an improved wash performance on egg stains
CA2685690A1 (en) 2007-04-30 2008-11-06 Danisco Us Inc. Use of protein hydrolysates to stabilize metalloprotease detergent formulations
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
EP2606146A1 (en) 2010-08-19 2013-06-26 Novozymes A/S Induced sporulation screening method
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
EP4199897A2 (en) 2020-08-24 2023-06-28 Novozymes A/S Oral care composition comprising a fructanase

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FR2423541A1 (en) * 1978-04-18 1979-11-16 Pasteur Institut Sorting of microorganisms, esp. bacteria - by lysis of individuals in colonies and detection of specific intracellular components
ATE25405T1 (en) * 1982-11-01 1987-02-15 Miles Lab PROCEDURE FOR DETECTING AND ISOLATING A MICROORGANISM.
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
JP2599946B2 (en) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド Subtilisin analog

Also Published As

Publication number Publication date
EP0260299A1 (en) 1988-03-23
WO1987005050A1 (en) 1987-08-27
JPS63502959A (en) 1988-11-02
EP0260299A4 (en) 1988-11-24
DK531887A (en) 1987-10-12

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Legal Events

Date Code Title Description
AHB Application shelved due to non-payment