FR2423541A1 - Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine - Google Patents

Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine

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Publication number
FR2423541A1
FR2423541A1 FR7811403A FR7811403A FR2423541A1 FR 2423541 A1 FR2423541 A1 FR 2423541A1 FR 7811403 A FR7811403 A FR 7811403A FR 7811403 A FR7811403 A FR 7811403A FR 2423541 A1 FR2423541 A1 FR 2423541A1
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France
Prior art keywords
microorganisms
colonies
individuals
bacteria
intracellular component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7811403A
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English (en)
Other versions
FR2423541B1 (fr
Inventor
Philippe Kourilsky
Brigitte Cami
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
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Institut Pasteur de Lille
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Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR7811403A priority Critical patent/FR2423541A1/fr
Publication of FR2423541A1 publication Critical patent/FR2423541A1/fr
Application granted granted Critical
Publication of FR2423541B1 publication Critical patent/FR2423541B1/fr
Granted legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé de tri parmi une population de micro-organismes, notamment bactéries, de certains d'entre eux qui sont susceptibles de produire un constituant intracellulaire repérable par une sonde. Il comprend l'induction dans une culture de ces micro-organismes, lorsque ceux-ci ont déjà formé des colonies, d'une transformation des conditions de la culture tendant à produire la lyse d'une partie des individus qui composent ces colonies, et le transfert sur un support des constituants intracellulaires libérés. On procède alors à la détection in situ avec la susdite sonde des constituants intracellulaires recherchés, d'où la possibilité de localiser sur le milieu de culture le micro-organisme recherché. Application à des isolements de gènes.
FR7811403A 1978-04-18 1978-04-18 Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine Granted FR2423541A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7811403A FR2423541A1 (fr) 1978-04-18 1978-04-18 Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7811403A FR2423541A1 (fr) 1978-04-18 1978-04-18 Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine

Publications (2)

Publication Number Publication Date
FR2423541A1 true FR2423541A1 (fr) 1979-11-16
FR2423541B1 FR2423541B1 (fr) 1980-10-31

Family

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Family Applications (1)

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FR7811403A Granted FR2423541A1 (fr) 1978-04-18 1978-04-18 Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine

Country Status (1)

Country Link
FR (1) FR2423541A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4492761A (en) * 1982-04-05 1985-01-08 Duke University Complement assay method
EP0168933A2 (fr) * 1984-06-05 1986-01-22 AMERSHAM INTERNATIONAL plc Détection et/ou identification de micro-organismes dans un échantillon par bioluminescence ou par d'autres marqueurs exogènes introduits génétiquement
EP0114668A3 (en) * 1983-01-20 1987-08-05 Integrated Genetics, Inc. Test for salmonella in food
EP0260299A1 (fr) * 1986-02-12 1988-03-23 Enzon Labs Inc. Procede de mutagenese et de criblage et produit obtenu

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NV217/77 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4492761A (en) * 1982-04-05 1985-01-08 Duke University Complement assay method
EP0114668A3 (en) * 1983-01-20 1987-08-05 Integrated Genetics, Inc. Test for salmonella in food
EP0168933A2 (fr) * 1984-06-05 1986-01-22 AMERSHAM INTERNATIONAL plc Détection et/ou identification de micro-organismes dans un échantillon par bioluminescence ou par d'autres marqueurs exogènes introduits génétiquement
EP0168933A3 (en) * 1984-06-05 1987-08-26 Shimon Ulitzur Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker
EP0260299A1 (fr) * 1986-02-12 1988-03-23 Enzon Labs Inc. Procede de mutagenese et de criblage et produit obtenu
EP0260299A4 (fr) * 1986-02-12 1988-11-24 Genex Corp Procede de mutagenese et de criblage et produit obtenu.

Also Published As

Publication number Publication date
FR2423541B1 (fr) 1980-10-31

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