EP0260299A4 - Verfahren zur mutagenesis und überprüfung und produkt. - Google Patents

Verfahren zur mutagenesis und überprüfung und produkt.

Info

Publication number
EP0260299A4
EP0260299A4 EP19870901901 EP87901901A EP0260299A4 EP 0260299 A4 EP0260299 A4 EP 0260299A4 EP 19870901901 EP19870901901 EP 19870901901 EP 87901901 A EP87901901 A EP 87901901A EP 0260299 A4 EP0260299 A4 EP 0260299A4
Authority
EP
European Patent Office
Prior art keywords
mutagenesis
product
screening method
screening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP19870901901
Other languages
English (en)
French (fr)
Other versions
EP0260299A1 (de
Inventor
Philip N Bryan
Michele L Rollence
Michael W Pantoliano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzon Labs Inc
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of EP0260299A1 publication Critical patent/EP0260299A1/de
Publication of EP0260299A4 publication Critical patent/EP0260299A4/de
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP19870901901 1986-02-12 1987-02-12 Verfahren zur mutagenesis und überprüfung und produkt. Ceased EP0260299A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82854586A 1986-02-12 1986-02-12
US828545 2010-07-01

Publications (2)

Publication Number Publication Date
EP0260299A1 EP0260299A1 (de) 1988-03-23
EP0260299A4 true EP0260299A4 (de) 1988-11-24

Family

ID=25252124

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19870901901 Ceased EP0260299A4 (de) 1986-02-12 1987-02-12 Verfahren zur mutagenesis und überprüfung und produkt.

Country Status (4)

Country Link
EP (1) EP0260299A4 (de)
JP (1) JPS63502959A (de)
DK (1) DK531887D0 (de)
WO (1) WO1987005050A1 (de)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE353250T1 (de) * 1987-04-06 1998-05-28 Novonordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0471410A3 (en) * 1990-08-15 1992-07-01 Unilever Nv Structured liquid detergent compositions containing subtilisin mutants
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
KR100258460B1 (ko) * 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DE69212390T2 (de) * 1991-05-01 1997-01-09 Unilever Nv Stabilisierte Enzyme enthaltende Waschmittelzusammensetzungen
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
GB9114734D0 (en) * 1991-07-09 1991-08-28 Univ London Process for modifying proteins
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
ATE409743T1 (de) * 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
WO1998020115A1 (en) 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
KR20010023468A (ko) 1997-08-29 2001-03-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 프로테아제 변이체 및 조성물
BR9811248B1 (pt) 1997-08-29 2011-10-04 variante de enzima subtilase derivada de uma subtilase originária selecionada a partir do sub-grupo i-s1 ou do sub-grupo i-s2, dita variante tendo melhorado desempenho de lavagem em detergentes em comparação com a subtilase originária, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processo para produzir uma variante, composição, uso de uma variante de subtilase.
DE19743683A1 (de) * 1997-10-02 1999-04-08 Basf Ag Verfahren zur Änderung der Substratspezifität von Enzymen
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
DE69830743T2 (de) 1997-11-21 2006-04-27 Novozymes A/S Protease-varianten und zusammensetzungen
JP2002507426A (ja) 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
WO2000071689A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
WO2000071691A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
ATE408680T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
WO2000071687A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
KR20020021397A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 규정된 에피토프 영역에 아미노산 결실 및 치환을 갖는서브틸리신 프로테아제 변이체
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
AU6085800A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Subtilisin protease variants having amino acid substitutions in defined epitope regions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6403692B1 (en) 2001-04-19 2002-06-11 Dow Global Technologies Inc. Filled thermoplastic composition
JP2004305175A (ja) 2003-04-10 2004-11-04 Kao Corp アルカリプロテアーゼ
BRPI0409992A (pt) 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
EP1678296B1 (de) 2003-10-23 2011-07-13 Novozymes A/S Protease mit verbesserter stabilität in detergentien
JP4787571B2 (ja) * 2004-10-08 2011-10-05 花王株式会社 アルカリプロテアーゼ
EP2007942B1 (de) 2006-04-14 2014-07-09 Danisco US Inc. Textilbehandlung in einem schritt
JP2009534022A (ja) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ 卵のシミに対する洗浄能力が向上したサビナーゼ変異体
MX2009011566A (es) 2007-04-30 2009-11-10 Danisco Us Inc Genencor Div Uso de hidrolizados de proteinas para estabilizar formulaciones detergentes que contienen metaloproteasa.
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
US20130224757A1 (en) 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
EP4199897A2 (de) 2020-08-24 2023-06-28 Novozymes A/S Mundpflegezusammensetzung mit einer fructanase

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (fr) * 1978-04-18 1979-11-16 Pasteur Institut Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine
EP0108303A1 (de) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Verfahren zum Nachweis und zur Isolation eines Mikroorganismus
EP0138075A1 (de) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Verfahren zur Bildung und zum Nachweis von Enzymvarianten mit erhöhter Thermostabilität und Aktivität
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2423541A1 (fr) * 1978-04-18 1979-11-16 Pasteur Institut Procede de tri parmi une population de micro-organismes, notamment de bacteries, de ceux qui sont porteurs ou producteurs d'un constituant intracellulaire particulier, notamment d'un fragment d'acide nucleique determine
EP0108303A1 (de) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Verfahren zum Nachweis und zur Isolation eines Mikroorganismus
EP0138075A1 (de) * 1983-09-16 1985-04-24 Synergen Associates, Inc. Verfahren zur Bildung und zum Nachweis von Enzymvarianten mit erhöhter Thermostabilität und Aktivität
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GENETIC TECHNOLOGY NEWS, vol. 6, no. 5, May 1986, pages 3-4; "Genex engineers detergent enzymes" *
J. CELL. BIOCHEM., SUPPL., no. 10, part A, 1986, page 271, no. E101; P. BRYAN et al.: "Protein engineering of subtilisin: proteases of enhanced stability", & SYMPOSIUM ON PROTEASES IN BIOLOGICAL CONTROL AND BIOTECHNOLOGY, 15TH ANNUAL UCLA, Los Angeles, 9th-15th February 1986 *
PROC. NATL. ACAD. SCI. USA, vol. 82, February 1985, pages 747-750; T. ALBER et al.: "A genetic screen for mutations that increase the thermal stability of phage T4 lysozyme" *
PROTEINS: STRUCTURE, FUNCTION AND GENETICS, vol. 1, 10th November 1986, pages 326-334; P.N. BRYAN et al.: "Proteases of enhanced stability: characterization of a thermostable variant of subtilisin" *
SCIENCE, vol. 229, no. 4719, 20th September 1985, pages 1193-1201; D. BOTSTEIN et al.: "Strategies and applications of in vitro mutagenesis" *
See also references of WO8705050A1 *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 05 December 1985, The American Society of Biological Chemists Inc., US; M. MATSUMURA et al., pp. 15298-15303# *
THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 260, 5th December 1985, pages 15298-15303, The American Society of Biological Chemists, Inc., US; M. MATSUMURA et al.: "Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus" *

Also Published As

Publication number Publication date
DK531887A (da) 1987-10-12
DK531887D0 (da) 1987-10-12
WO1987005050A1 (en) 1987-08-27
JPS63502959A (ja) 1988-11-02
EP0260299A1 (de) 1988-03-23

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