DE3856593D1 - Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen - Google Patents

Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen

Info

Publication number
DE3856593D1
DE3856593D1 DE3856593T DE3856593T DE3856593D1 DE 3856593 D1 DE3856593 D1 DE 3856593D1 DE 3856593 T DE3856593 T DE 3856593T DE 3856593 T DE3856593 T DE 3856593T DE 3856593 D1 DE3856593 D1 DE 3856593D1
Authority
DE
Germany
Prior art keywords
metal ion
ion binding
control
binding sites
electrostatic interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3856593T
Other languages
English (en)
Inventor
Michael W Pantoliano
Philip N Bryan
Barry C Finzel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3856593(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of DE3856593D1 publication Critical patent/DE3856593D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DE3856593T 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen Expired - Lifetime DE3856593D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06

Publications (1)

Publication Number Publication Date
DE3856593D1 true DE3856593D1 (de) 2007-10-04

Family

ID=21879773

Family Applications (3)

Application Number Title Priority Date Filing Date
DE3856593T Expired - Lifetime DE3856593D1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
DE3856362T Expired - Lifetime DE3856362T2 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE3856362T Expired - Lifetime DE3856362T2 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.

Country Status (6)

Country Link
EP (2) EP0916732B1 (de)
JP (2) JP3155984B2 (de)
AT (2) ATE371029T1 (de)
DE (3) DE3856593D1 (de)
DK (1) DK175917B1 (de)
WO (1) WO1988008028A1 (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
JPH04500384A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
ES2227508T3 (es) * 1989-06-26 2005-04-01 Unilever N.V. Composiciones detergentes enzimaticas.
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DE69133632D1 (de) * 1990-01-04 2010-05-27 Danisco Us Inc Glukose-Isomerasen mit geänderter Substratspezifität
ES2253733T3 (es) * 1990-01-04 2006-06-01 Genencor International, Inc. Nuevas glucosa isomerasas con un ph optimo alterado.
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
JP2888986B2 (ja) * 1993-10-14 1999-05-10 ザ、プロクター、エンド、ギャンブル、カンパニー プロテアーゼ酵素を含んでなる漂白組成物
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0687733A1 (de) * 1994-06-16 1995-12-20 The Procter & Gamble Company Detergenzusammensetzung die Wolle-kompatible-hochalkalische Proteasen enthält
US5922082A (en) 1994-06-16 1999-07-13 Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
KR100591553B1 (ko) 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
CA2270593C (en) 1996-11-04 2005-06-07 Novo Nordisk A/S Subtilase variants and compositions
JP2001514846A (ja) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ プロテアーゼ変異体及び組成物
CN1148444C (zh) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 蛋白酶变体及组合物
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
EP1032655B1 (de) 1997-11-21 2005-06-29 Novozymes A/S Protease-varianten und zusammensetzungen
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
CA2325568A1 (en) 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
WO1999049057A1 (en) * 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
AU4392900A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128
ATE407201T1 (de) 1999-05-20 2008-09-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
EP1183342B2 (de) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
AU5928400A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Protease conjugates having sterically protected clip sites
CA2376045A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
WO2001007575A2 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
WO2001027227A2 (en) * 1999-10-14 2001-04-19 Novozymes A/S Detergent compositions having improved wash performance comprising a subtilase variant and a surfactant
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) * 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
CA2526341C (en) 2003-05-07 2013-02-19 Novozymes A/S Variant subtilisin enzymes (subtilases)
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
JP2007521823A (ja) * 2004-02-13 2007-08-09 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
EP1598367A1 (de) * 2004-05-18 2005-11-23 ZLB Behring GmbH Modifizierte Gerinnungsfaktoren mit erhöhter Stabilität und deren Derivate
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
CN101460618A (zh) 2006-04-20 2009-06-17 诺维信公司 对蛋渍具有改进的洗涤性能的洒维奈斯变体
EP2147098B1 (de) 2007-04-30 2013-07-17 Danisco US Inc. Verwendung von proteinhydrolysaten zur stabilisierung von metalloprotease-detergens-formulierungen
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
WO2012022777A1 (en) 2010-08-19 2012-02-23 Novozymes A/S Induced sporulation screening method
WO2013004635A1 (en) 2011-07-01 2013-01-10 Novozymes A/S Liquid detergent composition
CN105189724A (zh) 2013-03-14 2015-12-23 诺维信公司 含有酶和抑制剂的水溶性膜
EP3461881A1 (de) 2013-05-03 2019-04-03 Novozymes A/S Mikroverkapselung von wasch- und reinigungsmittelenzymen
US20190002819A1 (en) 2015-12-28 2019-01-03 Novozymes Bioag A/S Heat priming of bacterial spores
WO2019063499A1 (en) 2017-09-27 2019-04-04 Novozymes A/S LIPASE VARIANTS AND MICROCAPSULE COMPOSITIONS COMPRISING SUCH VARIANTS OF LIPASE
WO2019154951A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipases, lipase variants and compositions thereof
CN111757930A (zh) 2018-02-08 2020-10-09 诺维信公司 脂肪酶变体及其组合物
EP3765185B1 (de) 2018-03-13 2023-07-19 Novozymes A/S Mikroverkapselung unter verwendung von aminozuckeroligomeren
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
EP3994255A1 (de) 2019-07-02 2022-05-11 Novozymes A/S Lipasevarianten und zusammensetzungen davon
JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
EP4199897A2 (de) 2020-08-24 2023-06-28 Novozymes A/S Mundpflegezusammensetzung mit einer fructanase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン

Also Published As

Publication number Publication date
JPH02502874A (ja) 1990-09-13
EP0353250B1 (de) 1999-09-08
DE3856362T2 (de) 2000-05-18
JP2001029076A (ja) 2001-02-06
EP0916732A1 (de) 1999-05-19
DK496489D0 (da) 1989-10-06
EP0353250A4 (en) 1990-09-05
ATE371029T1 (de) 2007-09-15
DK496489A (da) 1989-10-06
DK175917B1 (da) 2005-06-27
DE3856362D1 (de) 1999-10-14
EP0353250A1 (de) 1990-02-07
DE353250T1 (de) 1998-05-28
EP0916732B1 (de) 2007-08-22
WO1988008028A1 (en) 1988-10-20
ATE184316T1 (de) 1999-09-15
JP3155984B2 (ja) 2001-04-16

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