DE3856593D1 - Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen - Google Patents

Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen

Info

Publication number
DE3856593D1
DE3856593D1 DE3856593T DE3856593T DE3856593D1 DE 3856593 D1 DE3856593 D1 DE 3856593D1 DE 3856593 T DE3856593 T DE 3856593T DE 3856593 T DE3856593 T DE 3856593T DE 3856593 D1 DE3856593 D1 DE 3856593D1
Authority
DE
Germany
Prior art keywords
metal ion
ion binding
control
binding sites
electrostatic interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3856593T
Other languages
English (en)
Inventor
Michael W Pantoliano
Philip N Bryan
Barry C Finzel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3856593(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of DE3856593D1 publication Critical patent/DE3856593D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
DE3856593T 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen Expired - Lifetime DE3856593D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06

Publications (1)

Publication Number Publication Date
DE3856593D1 true DE3856593D1 (de) 2007-10-04

Family

ID=21879773

Family Applications (3)

Application Number Title Priority Date Filing Date
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.
DE3856593T Expired - Lifetime DE3856593D1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
DE3856362T Expired - Lifetime DE3856362T2 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3856362T Expired - Lifetime DE3856362T2 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Country Status (6)

Country Link
EP (2) EP0916732B1 (de)
JP (2) JP3155984B2 (de)
AT (2) ATE371029T1 (de)
DE (3) DE353250T1 (de)
DK (1) DK175917B1 (de)
WO (1) WO1988008028A1 (de)

Families Citing this family (77)

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DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
EP0405901B1 (de) * 1989-06-26 2004-09-01 Unilever Plc Enzymatische Waschmittelzusammensetzungen
EP0405902A1 (de) * 1989-06-26 1991-01-02 Unilever Plc Enzymatische Waschmittelzusammensetzungen
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DK1264883T3 (da) * 1990-01-04 2010-07-19 Danisco Us Inc Glucoseisomeraser med ændret substratspecificitet
ATE310080T1 (de) * 1990-01-04 2005-12-15 Genencor Int Glucose-isomerase mit geändertem ph-optimum
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
AU8079794A (en) * 1993-10-14 1995-05-04 Procter & Gamble Company, The Protease-containing cleaning compositions
CN1088102C (zh) * 1993-10-14 2002-07-24 普罗格特-甘布尔公司 含有蛋白酶的漂白组合物
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5922082A (en) 1994-06-16 1999-07-13 Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
EP0687733A1 (de) * 1994-06-16 1995-12-20 The Procter & Gamble Company Detergenzusammensetzung die Wolle-kompatible-hochalkalische Proteasen enthält
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
AU4772697A (en) 1996-11-04 1998-05-29 Novo Nordisk A/S Subtilase variants and compositions
ATE409743T1 (de) 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
BR9811412A (pt) 1997-08-29 2000-08-22 Novo Nordisk As Variante de enzima subtilase tendo melhorado desempenho de lavagem em detergentes, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processos para produzir uma variante e para identificar uma variante de protease, composição, e, uso de uma variante de subtilase
WO1999011768A1 (en) 1997-08-29 1999-03-11 Novo Nordisk A/S Protease variants and compositions
JP4246384B2 (ja) 1997-11-21 2009-04-02 ノボザイムス アクティーゼルスカブ プロテアーゼ変異体及び組成物
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
CA2325568A1 (en) 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
CN1303437A (zh) * 1998-03-26 2001-07-11 宝洁公司 有氨基酸缺失和替代的丝氨酸蛋白酶变体
WO2000071691A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
DE60040285D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
EP1183340B1 (de) 1999-05-20 2008-09-03 Novozymes A/S Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
ATE408680T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU4392700A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 129 and 130
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
BR0012693A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante, de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
AU7507900A (en) * 1999-10-14 2001-04-23 Novozymes A/S Detergent compositions having improved wash performance comprising a subtilase variant and a surfactant
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE602004016314D1 (de) 2003-05-07 2008-10-16 Maxygen Inc Enzymvarianten von subtilisin (subtilasen)
WO2005040372A1 (en) 2003-10-23 2005-05-06 Novozymes A/S Protease with improved stability in detergents
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
US20070281332A1 (en) * 2004-02-13 2007-12-06 Allan Svendsen Protease Variants
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
EP1598367A1 (de) * 2004-05-18 2005-11-23 ZLB Behring GmbH Modifizierte Gerinnungsfaktoren mit erhöhter Stabilität und deren Derivate
EP2007942B1 (de) 2006-04-14 2014-07-09 Danisco US Inc. Textilbehandlung in einem schritt
EP2013339B1 (de) 2006-04-20 2013-09-11 Novozymes A/S Savinase-varianten mit verbesserter waschleistung gegen eiflecken
BRPI0810863A2 (pt) 2007-04-30 2014-10-07 Danisco Us Inc Genencor Div Uso de hidrolisados de proteína para estabilizar formulações de detergente de metaloprotease.
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
EP2606146A1 (de) 2010-08-19 2013-06-26 Novozymes A/S Screeningverfahren für eingeleitete sporulation
CN103649289A (zh) 2011-07-01 2014-03-19 诺维信公司 液体洗涤剂组合物
WO2014152674A1 (en) 2013-03-14 2014-09-25 Novozymes A/S Enzyme and inhibitor containing water-soluble films
CN105164244B (zh) 2013-05-03 2019-08-20 诺维信公司 洗涤剂酶的微囊化
US20190002819A1 (en) 2015-12-28 2019-01-03 Novozymes Bioag A/S Heat priming of bacterial spores
CN117448299A (zh) 2017-09-27 2024-01-26 诺维信公司 脂肪酶变体和包含此类脂肪酶变体的微囊组合物
CN111868239A (zh) 2018-02-08 2020-10-30 诺维信公司 脂肪酶、脂肪酶变体及其组合物
WO2019154952A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipase variants and compositions thereof
WO2019175240A1 (en) 2018-03-13 2019-09-19 Novozymes A/S Microencapsulation using amino sugar oligomers
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
EP3994255A1 (de) 2019-07-02 2022-05-11 Novozymes A/S Lipasevarianten und zusammensetzungen davon
CN116322625A (zh) 2020-08-24 2023-06-23 诺维信公司 包含果聚糖酶的口腔护理组合物

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IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
EP0260299A4 (de) * 1986-02-12 1988-11-24 Genex Corp Verfahren zur mutagenesis und überprüfung und produkt.
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン

Also Published As

Publication number Publication date
ATE184316T1 (de) 1999-09-15
ATE371029T1 (de) 2007-09-15
EP0353250B1 (de) 1999-09-08
DE3856362T2 (de) 2000-05-18
DE3856362D1 (de) 1999-10-14
EP0353250A4 (en) 1990-09-05
EP0916732A1 (de) 1999-05-19
JPH02502874A (ja) 1990-09-13
JP3155984B2 (ja) 2001-04-16
EP0353250A1 (de) 1990-02-07
DK496489D0 (da) 1989-10-06
DK496489A (da) 1989-10-06
DE353250T1 (de) 1998-05-28
EP0916732B1 (de) 2007-08-22
DK175917B1 (da) 2005-06-27
JP2001029076A (ja) 2001-02-06
WO1988008028A1 (en) 1988-10-20

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Legal Events

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8332 No legal effect for de