DE3856362D1 - Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen - Google Patents

Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Info

Publication number
DE3856362D1
DE3856362D1 DE3856362T DE3856362T DE3856362D1 DE 3856362 D1 DE3856362 D1 DE 3856362D1 DE 3856362 T DE3856362 T DE 3856362T DE 3856362 T DE3856362 T DE 3856362T DE 3856362 D1 DE3856362 D1 DE 3856362D1
Authority
DE
Germany
Prior art keywords
proteins
metalion
stabilization
control
electrostatic interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3856362T
Other languages
English (en)
Other versions
DE3856362T2 (de
Inventor
Michael Pantoliano
Barry Finzel
Philip Bryan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3856362(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of DE3856362D1 publication Critical patent/DE3856362D1/de
Application granted granted Critical
Publication of DE3856362T2 publication Critical patent/DE3856362T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DE3856362T 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen Expired - Lifetime DE3856362T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06
PCT/US1988/001235 WO1988008028A1 (en) 1987-04-06 1988-04-06 The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins

Publications (2)

Publication Number Publication Date
DE3856362D1 true DE3856362D1 (de) 1999-10-14
DE3856362T2 DE3856362T2 (de) 2000-05-18

Family

ID=21879773

Family Applications (3)

Application Number Title Priority Date Filing Date
DE3856593T Expired - Lifetime DE3856593D1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.
DE3856362T Expired - Lifetime DE3856362T2 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE3856593T Expired - Lifetime DE3856593D1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
DE0353250T Pending DE353250T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen.

Country Status (6)

Country Link
EP (2) EP0353250B1 (de)
JP (2) JP3155984B2 (de)
AT (2) ATE184316T1 (de)
DE (3) DE3856593D1 (de)
DK (1) DK175917B1 (de)
WO (1) WO1988008028A1 (de)

Families Citing this family (77)

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US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DE69034159T2 (de) * 1989-06-26 2005-02-17 Unilever N.V. Enzymatische Waschmittelzusammensetzungen
WO1991000335A1 (en) * 1989-06-26 1991-01-10 Unilever Plc Enzymatic detergent compositions
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
EP0436502B1 (de) * 1990-01-04 2005-11-16 Genencor International, Inc. Glucose-Isomerase mit geändertem pH-Optimum
EP0440273B1 (de) * 1990-01-04 2003-04-09 Genencor International Inc. Eine Methode zur Herstellung von Glukose-Isomerasen mit geänderter Substratspezifität
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
ATE361355T1 (de) * 1993-10-14 2007-05-15 Procter & Gamble Proteasehaltige reinigungsmittel
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
CA2173106C (en) * 1993-10-14 2000-02-15 Michael Eugene Burns Bleaching compositions comprising protease enzymes
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0687733A1 (de) * 1994-06-16 1995-12-20 The Procter & Gamble Company Detergenzusammensetzung die Wolle-kompatible-hochalkalische Proteasen enthält
US5922082A (en) 1994-06-16 1999-07-13 Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
BR9712473B1 (pt) 1996-11-04 2009-08-11 variantes de subtilase e composições.
BR9712878A (pt) 1996-11-04 2000-02-01 Novo Nordisk As Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase.
CN1272137A (zh) 1997-08-29 2000-11-01 诺沃挪第克公司 蛋白酶变体及组合物
ATE385254T1 (de) 1997-08-29 2008-02-15 Novozymes As Proteasevarianten und zusammensetzungen
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
ATE298791T1 (de) 1997-11-21 2005-07-15 Novozymes As Protease-varianten und zusammensetzungen
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
JP2002518993A (ja) * 1998-03-26 2002-07-02 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸欠失および置換を有するセリンプロテアーゼ変異体
BR9909130A (pt) 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
ATE402996T1 (de) 1999-05-20 2008-08-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
AU4393000A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 128 and 129
AU4392800A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 126 and 127
AU4392500A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 132 and 133
WO2000071689A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
CZ2002171A3 (cs) 1999-07-22 2002-06-12 The Procter & Gamble Company Proteinázový konjugát, čistící prostředek a prostředek osobní péče
WO2001027227A2 (en) * 1999-10-14 2001-04-19 Novozymes A/S Detergent compositions having improved wash performance comprising a subtilase variant and a surfactant
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
CA2526341C (en) 2003-05-07 2013-02-19 Novozymes A/S Variant subtilisin enzymes (subtilases)
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
WO2005078074A2 (en) * 2004-02-13 2005-08-25 Novozymes A/S Protease variants
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
EP1598367A1 (de) * 2004-05-18 2005-11-23 ZLB Behring GmbH Modifizierte Gerinnungsfaktoren mit erhöhter Stabilität und deren Derivate
MX2008012999A (es) 2006-04-14 2008-10-17 Genencor Int Tratamiento de una etapa de textiles.
US20090215663A1 (en) 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
JP5427776B2 (ja) 2007-04-30 2014-02-26 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン メタロプロテアーゼ洗剤調合剤を安定化するためのタンパク質の加水分解物の使用
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
CN103154263A (zh) 2010-08-19 2013-06-12 诺维信公司 诱导的孢子形成筛选方法
BR112013033816A2 (pt) 2011-07-01 2017-02-14 Novozymes A / S composição de detergente líquido sem boro
EP2970830B1 (de) 2013-03-14 2017-12-13 Novozymes A/S Protease und hemmer enthaltend in wasserlöslichen filmen
CN105164244B (zh) 2013-05-03 2019-08-20 诺维信公司 洗涤剂酶的微囊化
EP3397061A1 (de) 2015-12-28 2018-11-07 Novozymes BioAG A/S Wärmebehandlung von bakteriensporen
EP3688150A1 (de) 2017-09-27 2020-08-05 Novozymes A/S Lipasevarianten und mikrokapselzusammensetzungen mit solchen lipasevarianten
CN111868239A (zh) 2018-02-08 2020-10-30 诺维信公司 脂肪酶、脂肪酶变体及其组合物
EP3749759A1 (de) 2018-02-08 2020-12-16 Novozymes A/S Lipasevarianten und zusammensetzungen davon
EP3765185B1 (de) 2018-03-13 2023-07-19 Novozymes A/S Mikroverkapselung unter verwendung von aminozuckeroligomeren
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
WO2021001400A1 (en) 2019-07-02 2021-01-07 Novozymes A/S Lipase variants and compositions thereof
US20230332124A1 (en) 2020-08-24 2023-10-19 Novozymes A/S Oral care composition comprising a fructanase

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NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
EP0260299A4 (de) * 1986-02-12 1988-11-24 Genex Corp Verfahren zur mutagenesis und überprüfung und produkt.
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins

Also Published As

Publication number Publication date
JP3155984B2 (ja) 2001-04-16
JPH02502874A (ja) 1990-09-13
DE353250T1 (de) 1998-05-28
WO1988008028A1 (en) 1988-10-20
DE3856362T2 (de) 2000-05-18
EP0353250B1 (de) 1999-09-08
EP0353250A1 (de) 1990-02-07
DK175917B1 (da) 2005-06-27
DK496489A (da) 1989-10-06
DK496489D0 (da) 1989-10-06
EP0916732A1 (de) 1999-05-19
EP0353250A4 (en) 1990-09-05
DE3856593D1 (de) 2007-10-04
ATE184316T1 (de) 1999-09-15
ATE371029T1 (de) 2007-09-15
EP0916732B1 (de) 2007-08-22
JP2001029076A (ja) 2001-02-06

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Owner name: NOVOZYMES A/S, BAGSVAERD, DK