IL85953A - Subtilisin analogs having improved stability and method for preparing such analogs - Google Patents

Subtilisin analogs having improved stability and method for preparing such analogs

Info

Publication number
IL85953A
IL85953A IL85953A IL8595388A IL85953A IL 85953 A IL85953 A IL 85953A IL 85953 A IL85953 A IL 85953A IL 8595388 A IL8595388 A IL 8595388A IL 85953 A IL85953 A IL 85953A
Authority
IL
Israel
Prior art keywords
analogs
subtilisin
improved stability
preparing
asn
Prior art date
Application number
IL85953A
Other languages
English (en)
Other versions
IL85953A0 (en
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21891131&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL85953(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of IL85953A0 publication Critical patent/IL85953A0/xx
Publication of IL85953A publication Critical patent/IL85953A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
IL85953A 1987-04-10 1988-03-31 Subtilisin analogs having improved stability and method for preparing such analogs IL85953A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/036,872 US4914031A (en) 1987-04-10 1987-04-10 Subtilisin analogs

Publications (2)

Publication Number Publication Date
IL85953A0 IL85953A0 (en) 1988-09-30
IL85953A true IL85953A (en) 1993-01-31

Family

ID=21891131

Family Applications (1)

Application Number Title Priority Date Filing Date
IL85953A IL85953A (en) 1987-04-10 1988-03-31 Subtilisin analogs having improved stability and method for preparing such analogs

Country Status (15)

Country Link
US (1) US4914031A (xx)
EP (1) EP0309565B2 (xx)
JP (1) JP2590247B2 (xx)
KR (1) KR960006120B1 (xx)
AT (1) ATE119941T1 (xx)
AU (1) AU612653B2 (xx)
CA (1) CA1316473C (xx)
DE (1) DE3853328T3 (xx)
DK (1) DK175576B1 (xx)
FI (1) FI105695B (xx)
HK (1) HK1007168A1 (xx)
IL (1) IL85953A (xx)
LV (1) LV11043B (xx)
NO (1) NO179679C (xx)
WO (1) WO1988008033A1 (xx)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
EP0353250B1 (en) * 1987-04-06 1999-09-08 Novo Nordisk A/S The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5246849A (en) * 1988-04-12 1993-09-21 Enzon, Inc. Thermally stable serine proteases
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
US5122449A (en) * 1988-10-07 1992-06-16 Eastman Kodak Company Use of a protease in the extraction of chlamydial, gonococcal and herpes antigens
WO1990014420A1 (en) * 1989-05-17 1990-11-29 Amgen Inc. Multiply mutated subtilisins
DK316989D0 (da) * 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
WO1991000334A1 (en) * 1989-06-26 1991-01-10 Unilever Plc Enzymatic detergent compositions
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
WO1991002792A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
IT1239733B (it) * 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
US5541062A (en) * 1990-02-23 1996-07-30 Arch Development Corporation Methods and compositions for preparing protein processing enzymes
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP0995801A1 (de) * 1991-07-27 2000-04-26 Genencor International GmbH Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
US5399484A (en) * 1991-10-08 1995-03-21 Eastman Kodak Company Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US5470733A (en) * 1993-06-01 1995-11-28 University Of Maryland Calcium free subtilisin mutants
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
CZ105396A3 (en) * 1993-10-14 1996-09-11 Procter & Gamble Cleaning agent, agent for cleaning fabrics, agent for washing dishes, washing agent, method of cleaning fabrics, method of washing dishes and washing process
CZ105296A3 (en) * 1993-10-14 1996-09-11 Procter & Gamble Bleaching agent containing protease enzymes, cleansing agents, agent for cleaning fabrics and fabric cleaning method
WO1995015371A1 (en) * 1993-12-03 1995-06-08 Buckman Laboratories International, Inc. Enzyme stabilization by block-copolymers
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
CN1136311C (zh) 1996-11-04 2004-01-28 诺沃奇梅兹有限公司 枯草杆菌酶变异体和组合物
EP2278001B1 (en) 1996-11-04 2013-10-23 Novozymes A/S Protease variants and compositions
CN100593036C (zh) 1997-08-29 2010-03-03 诺沃奇梅兹有限公司 蛋白酶变体及组合物
CN1272137A (zh) 1997-08-29 2000-11-01 诺沃挪第克公司 蛋白酶变体及组合物
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
EP1032655B1 (en) 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
AU2742499A (en) * 1998-03-26 1999-10-18 Procter & Gamble Company, The Serine protease variants having amino acid substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6642011B2 (en) * 1998-04-15 2003-11-04 Genencor International, Inc. Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
US6831053B1 (en) 1998-10-23 2004-12-14 The Procter & Gamble Company Bleaching compositions comprising multiply-substituted protease variants
US6376450B1 (en) * 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
ATE504651T1 (de) 1998-12-18 2011-04-15 Novozymes As Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion
EP1183343B2 (en) 1999-05-20 2013-11-27 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
ATE408679T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128
WO2000071688A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
WO2000071690A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
CA2379729A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Protease conjugates having sterically protected clip sites
CZ2002212A3 (cs) 1999-07-22 2002-07-17 The Procter & Gamble Company Varianty subtilisinové proteasy mající aminokyselinové substituce v definovaných epitopových oblastech
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
WO2001007575A2 (en) * 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
WO2002010183A1 (en) * 2000-07-31 2002-02-07 Menzel, Rolf Compositions and methods for directed gene assembly
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
CN100404673C (zh) 2001-02-19 2008-07-23 默克专利有限公司 鉴定t细胞表位的方法及制备具有降低的免疫原性的分子的用途
CN100489094C (zh) * 2001-03-23 2009-05-20 金克克国际有限公司 具有改变的免疫原性反应的蛋白质及制备和使用该蛋白质的方法
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
WO2003062380A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
EP2339017A3 (en) 2002-03-29 2011-10-12 Genencor International, Inc. Enhanced protein expression in bacillus
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
EP1623029B1 (en) * 2003-05-07 2008-09-03 Novozymes A/S Variant subtilisin enzymes (subtilases)
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
JP2007521823A (ja) * 2004-02-13 2007-08-09 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
JP2007532114A (ja) * 2004-04-09 2007-11-15 ジェネンコー・インターナショナル・インク バチルスにおけるｐｃｋＡ修飾および増強されたタンパク質発現
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
KR100750659B1 (ko) * 2005-02-18 2007-08-20 인하대학교 산학협력단 계면활성제 및 산화제에 매우 안정한 호염, 호알카리성 단백질 분해효소 및 이를 암호화하는 유전자
MX2008012999A (es) 2006-04-14 2008-10-17 Genencor Int Tratamiento de una etapa de textiles.
EP2013339B1 (en) 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
RU2496875C2 (ru) 2007-03-12 2013-10-27 ДАНИСКО ЮЭс ИНК. Мoдифицированные протеазы
JP5427776B2 (ja) 2007-04-30 2014-02-26 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン メタロプロテアーゼ洗剤調合剤を安定化するためのタンパク質の加水分解物の使用
ATE550348T1 (de) 2007-05-10 2012-04-15 Danisco Us Inc Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007051092A1 (de) * 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
KR20100098652A (ko) 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산
DK2257629T3 (en) * 2008-03-28 2016-06-13 Danisco Us Inc METHOD FOR LOCUSAMPLIFIKATION in a bacterial cell
JP5647976B2 (ja) * 2008-06-06 2015-01-07 ダニスコ・ユーエス・インク 変異体微生物プロテアーゼを含む組成物及び方法
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
CN103154263A (zh) 2010-08-19 2013-06-12 诺维信公司 诱导的孢子形成筛选方法
WO2013076269A1 (en) * 2011-11-25 2013-05-30 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10287591B2 (en) 2013-12-31 2019-05-14 Danisco Us Inc Enhanced protein expression
WO2016001450A2 (en) 2014-07-04 2016-01-07 Novozymes A/S Subtilase variants and polynucleotides encoding same
US10683528B2 (en) 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
KR20170093247A (ko) 2014-12-19 2017-08-14 다니스코 유에스 인크. 향상된 단백질 발현
US11866713B2 (en) 2017-02-24 2024-01-09 Danisco Us Inc. Compositions and methods for increased protein production in bacillus licheniformis
EP3655537A1 (en) 2017-08-23 2020-05-27 Danisco US Inc. Methods and compositions for efficient genetic modifications of bacillus licheniformis strains
CN111094576A (zh) 2017-09-13 2020-05-01 丹尼斯科美国公司 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
KR20200105879A (ko) 2018-01-03 2020-09-09 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
JP2023539234A (ja) 2020-08-24 2023-09-13 ノボザイムス アクティーゼルスカブ フルクタナーゼを含む口腔ケア組成物
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) * 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
JPS60160884A (ja) * 1984-02-01 1985-08-22 Showa Denko Kk 酵素安定化法
DE3685769T2 (de) * 1985-04-15 1993-01-21 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
JPH0657151B2 (ja) * 1986-05-15 1994-08-03 昭和電工株式会社 酵素の安定化法
JP2637532B2 (ja) * 1987-02-27 1997-08-06 ギスト ブロカデス ナームローゼ フェンノートチャップ 原核微生物の染色体ｄｎａにおける安定した遺伝子増幅
EP0353250B1 (en) * 1987-04-06 1999-09-08 Novo Nordisk A/S The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins

Also Published As

Publication number Publication date
EP0309565B2 (en) 2001-08-22
JP2590247B2 (ja) 1997-03-12
US4914031A (en) 1990-04-03
IL85953A0 (en) 1988-09-30
DE3853328T3 (de) 2002-06-06
FI885719A (fi) 1988-12-09
DK686788A (da) 1988-12-09
HK1007168A1 (en) 1999-04-01
EP0309565A4 (xx) 1990-02-05
WO1988008033A1 (en) 1988-10-20
LV11043A (lv) 1996-02-20
DK175576B1 (da) 2004-12-13
AU1701688A (en) 1988-11-04
DK686788D0 (da) 1988-12-09
JPH01502957A (ja) 1989-10-12
KR960006120B1 (ko) 1996-05-09
CA1316473C (en) 1993-04-20
FI885719A0 (fi) 1988-12-09
KR890700668A (ko) 1989-04-26
LV11043B (en) 1996-06-20
EP0309565A1 (en) 1989-04-05
NO885484D0 (no) 1988-12-09
NO179679C (no) 1996-11-27
NO885484L (no) 1989-02-09
EP0309565B1 (en) 1995-03-15
DE3853328D1 (de) 1995-04-20
FI105695B (fi) 2000-09-29
ATE119941T1 (de) 1995-04-15
NO179679B (no) 1996-08-19
AU612653B2 (en) 1991-07-18
DE3853328T2 (de) 1995-07-27

Similar Documents

Publication Publication Date Title
IL85953A (en) Subtilisin analogs having improved stability and method for preparing such analogs
DE69028040T2 (de) Mehrfach mutierte Subtilisine
PT95741A (pt) Processo para a preparacao de uma composicao de limpeza enzimatica de carbonil-hidrolase compreendendo subtilisina
NZ224992A (en) Factor viii analogues and their preparation
ES2141761T3 (es) Secuencias de dna codificantes de nuevos factores de crecimiento/diferenciacion.
BG101028A (en) Synthetic peptide analogs of lung surfactant protein sp-c
GR3017169T3 (en) Collagen compositions and methods for preparation thereof.
NZ503235A (en) Amino-terminally truncated RANTES as chemokine antagonists
IL81221A0 (en) Dna sequences coding for proteins having the biological activity of husi-type i inhibitors,biotechnological methods for preparing said proteins and pharmaceutical compositions containing them
HUT58765A (en) Process for producing peptide compounds and pharmaceutical compositions containing them as active components
HK1009151A1 (en) Mpl ligand analogs mpl.
EP0362860A3 (en) Alcohol-modified silicone ester derivative and cosmetic composition containing same
UA37217C2 (uk) Аналоги 15-дезоксиспергуаліну, спосіб їх одержання, фармацевтична композиція та проміжні сполуки
EP0367641A3 (en) Lymphokines, dna sequences encoding these lymphokines and pharmaceutical compositions containing these lymphokines

Legal Events

Date Code Title Description
KB Patent renewed
KB Patent renewed
EXP Patent expired