ES2743050T5 - Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra - Google Patents

Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra Download PDF

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ES2743050T5
ES2743050T5 ES13775206T ES13775206T ES2743050T5 ES 2743050 T5 ES2743050 T5 ES 2743050T5 ES 13775206 T ES13775206 T ES 13775206T ES 13775206 T ES13775206 T ES 13775206T ES 2743050 T5 ES2743050 T5 ES 2743050T5
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nucleic acid
target nucleic
acid molecule
target
amplification
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ES2743050T3 (es
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Daniel Shaffer
Stephen Judice
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Envirologix Inc
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Envirologix Inc
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Engineering & Computer Science (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES13775206T 2012-04-09 2013-04-09 Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra Active ES2743050T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261621975P 2012-04-09 2012-04-09
PCT/US2013/035750 WO2013155056A1 (en) 2012-04-09 2013-04-09 Compositions and methods for quantifying a nucleic acid sequence in a sample

Publications (2)

Publication Number Publication Date
ES2743050T3 ES2743050T3 (es) 2020-02-18
ES2743050T5 true ES2743050T5 (es) 2022-10-20

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ES19175608T Active ES3011408T3 (en) 2012-04-09 2013-04-09 Compositions and methods for quantifying a nucleic acid sequence in a sample
ES13775206T Active ES2743050T5 (es) 2012-04-09 2013-04-09 Composiciones y procedimientos para cuantificar una secuencia de ácido nucleico en una muestra

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US (8) US9096897B2 (enEXAMPLES)
EP (3) EP4524265A3 (enEXAMPLES)
JP (4) JP5801513B2 (enEXAMPLES)
KR (4) KR101570951B1 (enEXAMPLES)
CN (2) CN114134207A (enEXAMPLES)
AU (3) AU2013246080C1 (enEXAMPLES)
BR (1) BR112014025262A8 (enEXAMPLES)
CA (1) CA2869971C (enEXAMPLES)
DK (2) DK2836609T4 (enEXAMPLES)
ES (2) ES3011408T3 (enEXAMPLES)
FI (1) FI3587587T3 (enEXAMPLES)
IN (1) IN2014DN08831A (enEXAMPLES)
MX (2) MX338296B (enEXAMPLES)
NZ (2) NZ723570A (enEXAMPLES)
RU (2) RU2601129C2 (enEXAMPLES)
UA (2) UA116205C2 (enEXAMPLES)
WO (1) WO2013155056A1 (enEXAMPLES)
ZA (3) ZA201408116B (enEXAMPLES)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012021493A2 (en) 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
EP4524265A3 (en) 2012-04-09 2025-06-25 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
MX386087B (es) 2014-04-22 2025-03-18 Envirologix Inc Composiciones y metodos para mejorar y/o predecir la amplificacion de adn.
BR112016025349A2 (pt) * 2014-04-30 2017-12-12 Envirologix Inc composições e métodos para a detecção de huanglongbing
EP3207159B1 (en) * 2014-10-14 2019-11-20 Abbott Laboratories Sequence conversion and signal amplifier dna having poly dna spacer sequences and detection methods using same
CN107208137A (zh) * 2014-10-20 2017-09-26 龙公司 检测rna病毒的组合物和方法
CA2975236A1 (en) * 2015-01-30 2016-08-04 Envirologix, Inc. Compositions and methods for rapid detection of salmonella
CN107532206B (zh) * 2015-01-30 2022-08-05 一龙公司 底物分子
CA3021580A1 (en) * 2015-06-25 2016-12-29 Barry L. Merriman Biomolecular sensors and methods
WO2017014963A1 (en) * 2015-07-20 2017-01-26 Envirologix Inc. Compositions and methods for rapid detection of salmonella serovar d1
EP3368654A4 (en) * 2015-10-30 2019-06-05 Alere Inc. DETERMINATION OF POLYMORPHISMS BY ISOTHERMIC NUCLEIC ACID AMPLIFICATION
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
KR20250022239A (ko) 2016-01-28 2025-02-14 로스웰 엠이 아이엔씨. 대량 병렬 dna 시퀀싱 장치
US11624725B2 (en) 2016-01-28 2023-04-11 Roswell Blotechnologies, Inc. Methods and apparatus for measuring analytes using polymerase in large scale molecular electronics sensor arrays
CA3053103A1 (en) 2016-02-09 2017-08-17 Roswell Biotechnologies, Inc. Electronic label-free dna and genome sequencing
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
GB201611469D0 (en) * 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
CN110431148A (zh) 2017-01-10 2019-11-08 罗斯威尔生命技术公司 用于dna数据存储的方法和系统
KR102601324B1 (ko) 2017-01-19 2023-11-10 로스웰 바이오테크놀로지스 인코포레이티드 2차원 레이어 재료를 포함하는 솔리드 스테이트 시퀀싱 디바이스들
CN110546276A (zh) 2017-04-25 2019-12-06 罗斯威尔生命技术公司 用于分子传感器的酶电路
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
KR102606670B1 (ko) 2017-05-09 2023-11-24 로스웰 바이오테크놀로지스 인코포레이티드 분자 센서들을 위한 결합 프로브 회로들
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
TWI754652B (zh) * 2017-07-10 2022-02-11 英商盧米瑞德克斯英國有限公司 核酸擴增方法中或相關之改良
EP3676389A4 (en) 2017-08-30 2021-06-02 Roswell Biotechnologies, Inc PROCESSIVE ENZYMATIC MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
WO2019046610A1 (en) 2017-08-31 2019-03-07 Ionian Technologies Inc. REACTION OF SYNCHRONIZATION AMPLIFICATION AND (NEAR) EXTENSION OF SYNCYTIAL RESPIRATORY VIRUS SPECIES
US11100404B2 (en) 2017-10-10 2021-08-24 Roswell Biotechnologies, Inc. Methods, apparatus and systems for amplification-free DNA data storage
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN108588252A (zh) * 2018-03-29 2018-09-28 杭州泰熙生物技术有限公司 一种寄生虫检测试剂盒及其检测方法
US12319712B2 (en) 2018-05-09 2025-06-03 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photoactivated agents
JP2021528091A (ja) * 2018-06-26 2021-10-21 ザ・ブロード・インスティテュート・インコーポレイテッド Crisprダブルニッカーゼに基づく増幅の組成物、システム、及び方法
GB201812149D0 (en) 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method
KR102154628B1 (ko) 2019-01-03 2020-09-10 베트올 (주) 반정량 수평류 면역진단 기술을 이용한 랄스토니아 솔라나세아룸과 푸사리움 옥시스포룸의 동시 진단 방법
KR102199394B1 (ko) * 2019-07-01 2021-01-06 한국화학연구원 이중분자비콘과 산화그래핀을 포함하는 핵산 검출용 조성물 및 이를 이용한 핵산 검출의 비색신호 증폭 방법
KR102531590B1 (ko) * 2019-07-16 2023-05-11 고려대학교 산학협력단 절단효소 인식부위의 미러링 결합에 의한 표적핵산의 검출 방법 및 핵산 검출용 조성물
CN110408678A (zh) * 2019-07-26 2019-11-05 宁儿医院股份有限公司 单管型多重微生物检测体系及其即时检测方法
WO2021226205A1 (en) * 2020-05-07 2021-11-11 Bioskryb, Inc. Detection of low abundance nucleic acids
WO2022169846A1 (en) * 2021-02-02 2022-08-11 Rhodx, Inc. Synthetic polynucleotides and methods for selectively amplifying alleles
EP4444907A1 (en) * 2021-12-08 2024-10-16 Massachusetts Institute of Technology Cold-temperature isothermal amplification of polynucleotides
WO2023221939A1 (en) * 2022-05-14 2023-11-23 Jiangsu Code Biomedical Technology Co., Ltd. Novel compositions and methods for cell-free dna detection
EP4547865A1 (en) * 2022-07-01 2025-05-07 Sherlock Biosciences, Inc. Ambient temperature nucleic acid amplification and detection
EP4565720A1 (en) 2022-08-04 2025-06-11 Abbott Laboratories Assays for detecting monkeypox virus
WO2025049451A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting cytomegalovirus (cmv) or epstein-bar virus (ebv)
WO2025049450A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting bk virus (bkv)

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5629179A (en) 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
CA2249717A1 (en) * 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
AU726821B2 (en) * 1996-07-16 2000-11-23 Gen-Probe Incorporated Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific Tm
GB9716231D0 (en) 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US6355421B1 (en) 1997-10-27 2002-03-12 Boston Probes, Inc. Methods, kits and compositions pertaining to PNA molecular beacons
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
WO2000028084A1 (en) 1998-11-06 2000-05-18 Molecular Biology Resources, Inc. Isothermal nucleic acid amplification methods
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
WO2001090415A2 (en) * 2000-05-20 2001-11-29 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
ES2254306T3 (es) * 2000-10-25 2006-06-16 F. Hoffmann-La Roche Ag Amplificacion usando cebadores modificados.
CA2433330A1 (en) 2000-12-27 2002-07-25 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
WO2003008622A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Exponential nucleic acid amplification using nicking endonucleases
CA2492220C (en) 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
JPWO2003016569A1 (ja) * 2001-08-20 2004-12-02 タカラバイオ株式会社 核酸の増幅方法
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20030211483A1 (en) 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
JP4380305B2 (ja) 2003-11-21 2009-12-09 味の素株式会社 発酵法によるl−アミノ酸の製造法
US20090017452A1 (en) 2004-03-05 2009-01-15 University Of Chicago Methods and compositions relating to the pharmacogenetics of different gene variants
US20060115838A1 (en) 2004-10-19 2006-06-01 Trevigen Inc. Real-time detection of amplicons using nucleic acid repair enzymes
JP5068175B2 (ja) * 2005-01-04 2012-11-07 日立化成工業株式会社 プライマー生成ローリングサークル型増幅
JP4555136B2 (ja) * 2005-03-31 2010-09-29 本田技研工業株式会社 燃料電池の電気システム、燃料電池車両及び電力供給方法
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7947286B2 (en) 2005-10-07 2011-05-24 Panthera Biopharma Llc Drosophila cell lines producing recombinant secretable filovirus surface glycoproteins lacking the membrane spanning domain
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
US20080096257A1 (en) 2006-08-15 2008-04-24 Zuxu Yao Methods for Rapid, Single-Step Strand Displacement Amplification of Nucleic Acids
DE102006039396A1 (de) 2006-08-22 2008-03-13 Robert Bosch Gmbh Laserlichtquelle
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
JP2010505396A (ja) 2006-10-06 2010-02-25 ディーエヌエー ジェノテック インク リボ核酸を抽出するための安定化組成物および方法
JP2008136451A (ja) 2006-12-05 2008-06-19 Hitachi Ltd 核酸増幅法
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
US20090081760A1 (en) 2007-01-12 2009-03-26 Monsanto Technology Llc Dmo methods and compositions
US20080254458A1 (en) 2007-04-10 2008-10-16 Asiagen Corporation Method of pre-treating in pleural effusion for detection of Mycobacterium tuberculosis
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2009020609A2 (en) 2007-08-06 2009-02-12 Nanogen, Inc. Isolation of nucleic acids molecules using modified solid supports
US20090197254A1 (en) 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
US20090325169A1 (en) 2008-04-30 2009-12-31 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2010026933A1 (ja) 2008-09-03 2010-03-11 タカラバイオ株式会社 Rna検出用組成物
AU2010206492B2 (en) 2009-01-21 2012-07-12 Human Genetic Signatures Pty Ltd Improved isothermal strand displacement amplification
WO2010107946A2 (en) 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
WO2012021493A2 (en) * 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
EP2420579A1 (en) * 2010-08-17 2012-02-22 QIAGEN GmbH Helicase dependent isothermal amplification using nicking enzymes
ES2684525T3 (es) 2011-09-15 2018-10-03 David A. Shafer Composiciones de sonda:antisonda para detección de ADN o ARN de alta especificidad
US20130217071A1 (en) 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
EP4524265A3 (en) 2012-04-09 2025-06-25 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
JP6382189B2 (ja) 2012-06-29 2018-08-29 ゼネラル・エレクトリック・カンパニイ Rnaテンプレートから開始する等温dna増幅用のキット
JP2014082936A (ja) 2012-10-19 2014-05-12 Kyoto Univ 変異型逆転写酵素
MX386087B (es) 2014-04-22 2025-03-18 Envirologix Inc Composiciones y metodos para mejorar y/o predecir la amplificacion de adn.
BR112016025349A2 (pt) 2014-04-30 2017-12-12 Envirologix Inc composições e métodos para a detecção de huanglongbing
CN107208137A (zh) 2014-10-20 2017-09-26 龙公司 检测rna病毒的组合物和方法
CA2965657A1 (en) 2014-10-28 2016-05-06 Envirologix Inc. Sample preparation vessels, microfluidic circuits, and systems and methods for sample preparation, extraction, and analysis
CA2975236A1 (en) 2015-01-30 2016-08-04 Envirologix, Inc. Compositions and methods for rapid detection of salmonella

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