ES2529204T3 - Métodos para la amplificación de ácido nucleico - Google Patents

Métodos para la amplificación de ácido nucleico Download PDF

Info

Publication number
ES2529204T3
ES2529204T3 ES10187807.2T ES10187807T ES2529204T3 ES 2529204 T3 ES2529204 T3 ES 2529204T3 ES 10187807 T ES10187807 T ES 10187807T ES 2529204 T3 ES2529204 T3 ES 2529204T3
Authority
ES
Spain
Prior art keywords
probe
pcr
amplification
initiator
late
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES10187807.2T
Other languages
English (en)
Spanish (es)
Inventor
Lawrence J. Wangh
John Rice
J. Aquiles Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Cristina Hartshorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36203686&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2529204(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Brandeis University filed Critical Brandeis University
Application granted granted Critical
Publication of ES2529204T3 publication Critical patent/ES2529204T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ES10187807.2T 2004-10-18 2005-10-17 Métodos para la amplificación de ácido nucleico Expired - Lifetime ES2529204T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
US619654P 2004-10-18

Publications (1)

Publication Number Publication Date
ES2529204T3 true ES2529204T3 (es) 2015-02-17

Family

ID=36203686

Family Applications (3)

Application Number Title Priority Date Filing Date
ES10187807.2T Expired - Lifetime ES2529204T3 (es) 2004-10-18 2005-10-17 Métodos para la amplificación de ácido nucleico
ES05810422T Expired - Lifetime ES2359322T3 (es) 2004-10-18 2005-10-17 Procedimiento para la amplificación de ácidos nucleicos.
ES14195859.5T Expired - Lifetime ES2668467T3 (es) 2004-10-18 2005-10-17 Métodos de amplificación LATE y secuenciación de un ácido nucleico

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES05810422T Expired - Lifetime ES2359322T3 (es) 2004-10-18 2005-10-17 Procedimiento para la amplificación de ácidos nucleicos.
ES14195859.5T Expired - Lifetime ES2668467T3 (es) 2004-10-18 2005-10-17 Métodos de amplificación LATE y secuenciación de un ácido nucleico

Country Status (17)

Country Link
US (3) US7632642B2 (enExample)
EP (3) EP2351766B1 (enExample)
JP (2) JP5543065B2 (enExample)
KR (3) KR101447405B1 (enExample)
CN (1) CN101076607B (enExample)
AT (1) ATE494295T1 (enExample)
AU (1) AU2005295298B2 (enExample)
BR (1) BRPI0517105B1 (enExample)
CA (3) CA2855748C (enExample)
DE (1) DE602005025791D1 (enExample)
DK (1) DK1805199T3 (enExample)
ES (3) ES2529204T3 (enExample)
NO (1) NO20072506L (enExample)
PL (1) PL1805199T3 (enExample)
PT (1) PT1805199E (enExample)
RU (1) RU2460804C2 (enExample)
WO (1) WO2006044994A2 (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2529204T3 (es) 2004-10-18 2015-02-17 Brandeis University Métodos para la amplificación de ácido nucleico
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
CA2693751A1 (en) 2007-07-12 2009-01-15 Smiths Detection Inc. Fiber optic detection system
EP2215259A1 (en) * 2007-10-23 2010-08-11 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
EP2382324A1 (en) 2008-12-30 2011-11-02 STMicroelectronics S.r.l. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
JP5757885B2 (ja) * 2009-03-12 2015-08-05 ブランディーズ・ユニバーシティ Ｐｃｒ用の試薬および方法
US8674080B2 (en) 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
JP5499037B2 (ja) * 2009-08-14 2014-05-21 株式会社日立製作所 遺伝子配列解析法及び試薬
US9353407B2 (en) 2009-10-21 2016-05-31 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
CN102712955A (zh) * 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
EP2569447A4 (en) * 2010-05-14 2013-11-27 Fluidigm Corp ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
WO2012033875A2 (en) 2010-09-08 2012-03-15 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP3135775B1 (en) 2010-11-10 2018-06-27 Brandeis University Kit for detecting and identifying mycobacteria
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
EP2649201A4 (en) * 2010-12-10 2014-10-01 Univ Brandeis COMPOSITIONS AND METHODS FOR DETECTING AND ANALYZING AFRICAN SWINE FEVER
CA2823193C (en) 2010-12-27 2017-06-06 Ibis Biosciences, Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
EP2756101B1 (en) 2011-09-15 2018-05-23 David A. Shafer Probe: antiprobe compositions for high specificity dna or rna detection
EP2774995A4 (en) * 2011-10-31 2015-04-29 Arkray Inc METHOD OF MEASURING THE ABUNDANCE OF GENES
EP4361607A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
EP2820157B1 (en) 2012-03-02 2019-05-01 Winthrop-University Hospital Method for using probe based pcr detection to measure the levels of circulating demethylated beta cell derived dna as a measure of beta cell loss in diabetes
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
EP2880184B1 (en) * 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CA2885195A1 (en) * 2012-09-17 2014-03-20 Brandeis University Combination of dsdna binding dye and probes for characterization of ssdna sequences
US20160186267A1 (en) * 2013-02-21 2016-06-30 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
CN105378160B (zh) 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
WO2015047186A1 (en) * 2013-09-30 2015-04-02 Di Wu Methods to profile molecular complexes by using proximity bar-coding
EP3080291B1 (en) 2013-12-11 2020-02-26 Qiagen GmbH Nucleic acid detection and quantification
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
LT3957753T (lt) 2014-10-10 2025-01-10 Rutgers, The State University Of New Jersey Polimerazės grandininės reakcijos pradmenys ir zondai, skirti mycobacterium tuberculosis
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
WO2016100335A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
AU2015373998A1 (en) 2014-12-31 2017-06-29 Visby Medical, Inc. Devices and methods for molecular diagnostic testing
CN107636165A (zh) * 2015-01-12 2018-01-26 生命科技公司 用于核酸文库质量控制和定量的方法、系统和其组合物
WO2017052300A1 (en) * 2015-09-24 2017-03-30 Seegene, Inc. . Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087942A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting rna and dna sequences comprising high levels of intramolecular hybridization
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
KR102326604B1 (ko) * 2016-06-02 2021-11-15 주식회사 씨젠 신호 변화량 데이터 세트를 이용한 샘플 내 타겟 분석물질 검출 방법
EP3478857A1 (en) 2016-06-29 2019-05-08 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
WO2018190814A1 (en) * 2017-04-11 2018-10-18 Nugen Technologies, Inc. Library quantitation and qualification
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
EP3676400A4 (en) * 2017-08-31 2021-06-02 Seegene, Inc. EVALUATING THE PERFORMANCE OF COMPONENTS USING A PAIR OF DIMERING PRIMERS
JP7239568B2 (ja) 2017-11-09 2023-03-14 ビスビュー メディカル，インコーポレイテッド 携帯型分子診断デバイスおよび標的ウイルスの検出方法
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅（武汉）生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
CN111629831A (zh) * 2018-01-22 2020-09-04 卢米耐克斯公司 用于离散解链分析的方法和组合物
EP3781710A4 (en) 2018-04-17 2022-01-05 Chromacode, Inc. METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS
AU2019255933B2 (en) 2018-04-20 2022-08-25 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
AU2019337088A1 (en) 2018-09-03 2021-05-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
JP2022530323A (ja) 2019-04-28 2022-06-29 ビスビュー メディカル，インコーポレイテッド デジタル検出能力および無線接続性を有する分子診断デバイス
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
CA3155451A1 (en) 2019-09-23 2021-04-01 Universiteit Gent Probe and method for str-genotyping
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术（深圳）有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
JPWO2023248310A1 (enExample) * 2022-06-20 2023-12-28

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
AU726501B2 (en) * 1996-06-04 2000-11-09 University Of Utah Research Foundation Monitoring hybridization during PCR
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis DETECTION OF NUCLEIC ACIDS
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
JP4919568B2 (ja) * 1999-10-22 2012-04-18 ピーエイチアールアイ プロパティーズ，インコーポレイテッド 短い配列の変異体を検出するためのアッセイ
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
WO2002101004A2 (en) * 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd Low-temperature cycle extension of dna with high priming specificity
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
EP1444365A4 (en) 2001-10-25 2005-07-20 Gorilla Genomics Inc ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS
AU2002346484A1 (en) 2001-11-21 2003-06-10 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
US7659060B2 (en) * 2002-09-02 2010-02-09 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphism
AU2003297297A1 (en) * 2002-11-22 2004-06-18 North Shore-Long Island Jewish Research Institute Elf3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
JP5162133B2 (ja) * 2003-12-03 2013-03-13 アボット・ラボラトリーズ 二本鎖直鎖核酸プローブ及びその使用
EP2439285B1 (en) * 2004-03-31 2019-05-08 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
ES2529204T3 (es) * 2004-10-18 2015-02-17 Brandeis University Métodos para la amplificación de ácido nucleico

Also Published As

Publication number Publication date
JP5860484B2 (ja) 2016-02-16
EP1805199B1 (en) 2011-01-05
KR101447405B1 (ko) 2014-10-06
KR20130021465A (ko) 2013-03-05
DK1805199T3 (da) 2011-04-26
JP2008516612A (ja) 2008-05-22
EP1805199A2 (en) 2007-07-11
EP2351766A2 (en) 2011-08-03
CA2996947A1 (en) 2006-04-27
BRPI0517105A (pt) 2008-09-30
US20130095479A1 (en) 2013-04-18
AU2005295298A1 (en) 2006-04-27
CA2584569C (en) 2014-09-30
ES2668467T3 (es) 2018-05-18
DE602005025791D1 (de) 2011-02-17
JP5543065B2 (ja) 2014-07-09
JP2014110793A (ja) 2014-06-19
KR20070090159A (ko) 2007-09-05
ATE494295T1 (de) 2011-01-15
CA2855748A1 (en) 2006-04-27
US9745624B2 (en) 2017-08-29
BRPI0517105B1 (pt) 2015-06-23
PL1805199T3 (pl) 2011-06-30
PT1805199E (pt) 2011-04-11
RU2007118545A (ru) 2008-11-27
NO20072506L (no) 2007-07-11
US20060177841A1 (en) 2006-08-10
CA2855748C (en) 2018-08-28
ES2359322T3 (es) 2011-05-20
EP2351766B1 (en) 2014-12-03
EP2927238A1 (en) 2015-10-07
KR20130113541A (ko) 2013-10-15
CN101076607B (zh) 2013-04-24
KR101424812B1 (ko) 2014-08-07
AU2005295298B2 (en) 2011-07-14
EP2927238B1 (en) 2018-01-17
HK1106251A1 (en) 2008-03-07
WO2006044994A3 (en) 2007-03-08
CA2996947C (en) 2019-02-26
EP1805199A4 (en) 2008-07-16
RU2460804C2 (ru) 2012-09-10
US20120040352A1 (en) 2012-02-16
EP2351766A3 (en) 2012-03-21
US7632642B2 (en) 2009-12-15
WO2006044994A2 (en) 2006-04-27
CN101076607A (zh) 2007-11-21
KR101347951B1 (ko) 2014-01-07
CA2584569A1 (en) 2006-04-27

Similar Documents

Publication Publication Date Title
ES2529204T3 (es) Métodos para la amplificación de ácido nucleico
JP5138141B2 (ja) 核酸の検出用物質及び検出方法
JP2018520641A (ja) 多重侵入切断アッセイ
KR20040024556A (ko) 검출 시스템
HK1216762A1 (zh) 用於表徵ssdna序列的dsdna結合染料和探針的組合
CN120442758A (zh) 可温度选择的fret盒信号传导
CA2896616C (en) Improved calibration of high resolution melting
KR20240049288A (ko) 핵산 서열의 동시다중 검출을 위한 장치 및 방법
HK1106251B (en) Methods for nucleic acid amplification