ES2040199T3 - Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos. - Google Patents

Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.

Info

Publication number
ES2040199T3
ES2040199T3 ES90306001T ES90306001T ES2040199T3 ES 2040199 T3 ES2040199 T3 ES 2040199T3 ES 90306001 T ES90306001 T ES 90306001T ES 90306001 T ES90306001 T ES 90306001T ES 2040199 T3 ES2040199 T3 ES 2040199T3
Authority
ES
Spain
Prior art keywords
sample
nucleic acid
amplified
amplification
acid amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90306001T
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English (en)
Other versions
ES2040199T1 (es
Inventor
James L Hartley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23416675&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2040199(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Publication of ES2040199T1 publication Critical patent/ES2040199T1/es
Application granted granted Critical
Publication of ES2040199T3 publication Critical patent/ES2040199T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UN PROCEDIMIENTO DE AMPLIFICACION SE REALIZA SOBRE UNA PRIMERA MUESTRA EN LA CUAL UNO O UNAS DE LOS CUATRO RIBONUCLEOSIDOS TRIFOSFATOS (RNTPS) O DEOXIRIBONUCLEOSIDOS TRIFOSFATOS (DNTPS) SON REEMPLAZADOS POR UNA EXO-MUESTRA NUCLEOTIDA QUE NO ESTA PRESENTE EN EL ACIDO NUCLEICO PARA SER AMPLIFICADO. TRAS ESTA AMPLIFICACION CUALQUIER PRODUCTO AMPLIFICADO CONTAMINANTE QUE PUEDA QUEDAR SOBRANTE ES SUJETO A UN TRATAMIENTO FISICO, QUIMICO, ENZIMATICO O BIOLOGICO QUE CEDE UN ACIDO NULEICO QUE CONTIENE LA EXO-MUESTRA NUCLEOTIDA SUSTANCIALMENTE INAMPLIFICABLE. EL TRATAMIENTO SE PUEDE REALIZAR COMO UN PASO SEPARADO O EN PRESENCIA DE UNA SEGUNDA MUESTRA QUE CONTENGA SECUENCIAS DE ACIDO NUCLEICO PARA SER AMPLIFICADAS. LAS SECUENCIAS YA AMPLIFICADAS DE LA PRIMERA MUESTRA NO LO SON DURANTE LA AMPLIFICACION DE LAS SECUENCIAS DE LA SEGUNDA MUESTRA
ES90306001T 1989-06-01 1990-06-01 Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos. Expired - Lifetime ES2040199T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/360,120 US5035996A (en) 1989-06-01 1989-06-01 Process for controlling contamination of nucleic acid amplification reactions

Publications (2)

Publication Number Publication Date
ES2040199T1 ES2040199T1 (es) 1993-10-16
ES2040199T3 true ES2040199T3 (es) 1995-11-01

Family

ID=23416675

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90306001T Expired - Lifetime ES2040199T3 (es) 1989-06-01 1990-06-01 Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.

Country Status (9)

Country Link
US (2) US5035996A (es)
EP (1) EP0401037B1 (es)
JP (1) JPH074248B2 (es)
AT (1) ATE127855T1 (es)
CA (1) CA2017522C (es)
DE (2) DE69022291T2 (es)
DK (1) DK0401037T3 (es)
ES (1) ES2040199T3 (es)
GR (2) GR920300019T1 (es)

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* Cited by examiner, † Cited by third party
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DE69022291D1 (de) 1995-10-19
CA2017522A1 (en) 1990-12-01
GR920300019T1 (en) 1992-08-25
DE69022291T2 (de) 1996-03-07
ATE127855T1 (de) 1995-09-15
US7687247B1 (en) 2010-03-30
JPH074248B2 (ja) 1995-01-25
DK0401037T3 (da) 1996-02-05
JPH0358785A (ja) 1991-03-13
DE401037T1 (de) 1992-03-19
EP0401037B1 (en) 1995-09-13
EP0401037A3 (en) 1991-09-18
CA2017522C (en) 1996-06-18
ES2040199T1 (es) 1993-10-16
GR3018005T3 (en) 1996-02-29
US5035996A (en) 1991-07-30

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