EA201890696A1 - Варианты гфпд и способы применения - Google Patents

Варианты гфпд и способы применения

Info

Publication number
EA201890696A1
EA201890696A1 EA201890696A EA201890696A EA201890696A1 EA 201890696 A1 EA201890696 A1 EA 201890696A1 EA 201890696 A EA201890696 A EA 201890696A EA 201890696 A EA201890696 A EA 201890696A EA 201890696 A1 EA201890696 A1 EA 201890696A1
Authority
EA
Eurasian Patent Office
Prior art keywords
gppd
inhibitors
polypeptides
herbicides
mutant
Prior art date
Application number
EA201890696A
Other languages
English (en)
Inventor
Марк Линка
Фабьен Поре
Бернд Лабер
Гудрун Ланге
Ян Теббе
Уэйн Коко
Михаэль Штрерат
Эрнст Вебер
Николаус Павловски
Зандра Геске
Хайке Бальфен-Росс
Нина Вобст
Кристина Тис
Мэньюэл Дьюбалд
Original Assignee
Байер Кропсайенс Акциенгезельшафт
Байер Кропсайенс Лп
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Байер Кропсайенс Акциенгезельшафт, Байер Кропсайенс Лп filed Critical Байер Кропсайенс Акциенгезельшафт
Publication of EA201890696A1 publication Critical patent/EA201890696A1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11027—4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27)

Abstract

В настоящем изобретении описаны полипептиды ГФПД и содержащие их растения, проявляющие полную устойчивость к одному или большему количеству гербицидов, ингибирующих ГФПД, принадлежащих к различным химическим классам. Разработан набор мутантных полипептидов ГФПД, которые не обладают или обладают только существенно сниженной аффинностью с гербицидами, ингибирующими ГФПД, и в то же самое время скорость диссоциации ингибиторов ГФПД мутантного полипептида ГФПД повышена до такой степени, что ингибиторы ГФПД больше не действуют как медленно связывающие или медленно, прочно связывающие ингибиторы, а вместо этого стали полностью обратимыми ингибиторами. В частности, обеспечены выделенные полинуклеотиды, кодирующие мутантные полипептиды ГФПД, придающие устойчивость к гербицидам, ингибирующим ГФПД, которые принадлежат к различным химическим классам. Дополнительно охвачены аминокислотные последовательности, соответствующие полинуклеотидам.
EA201890696A 2015-09-11 2016-09-08 Варианты гфпд и способы применения EA201890696A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15184866 2015-09-11
PCT/EP2016/071159 WO2017042259A1 (en) 2015-09-11 2016-09-08 Hppd variants and methods of use

Publications (1)

Publication Number Publication Date
EA201890696A1 true EA201890696A1 (ru) 2018-09-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201890696A EA201890696A1 (ru) 2015-09-11 2016-09-08 Варианты гфпд и способы применения

Country Status (17)

Country Link
US (1) US10597674B2 (ru)
EP (1) EP3347475B1 (ru)
JP (1) JP6873979B2 (ru)
KR (1) KR20180043838A (ru)
CN (1) CN108884470B (ru)
AR (1) AR105984A1 (ru)
AU (1) AU2016319093B2 (ru)
BR (1) BR112018004779A8 (ru)
CA (1) CA2998036A1 (ru)
EA (1) EA201890696A1 (ru)
ES (1) ES2933673T3 (ru)
HK (1) HK1258349A1 (ru)
MX (1) MX2018003044A (ru)
UA (1) UA126550C2 (ru)
UY (1) UY36898A (ru)
WO (1) WO2017042259A1 (ru)
ZA (1) ZA201802329B (ru)

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CA3055396A1 (en) * 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
BR112019018056A2 (pt) * 2017-03-07 2020-08-11 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos
BR112019018175A2 (pt) * 2017-03-07 2020-04-07 BASF Agricultural Solutions Seed US LLC molécula, célula, planta, sementes, polipeptídeos recombinantes, método para produzir um polipeptídeo, planta, método para controlar ervas, uso do ácido nucleico e produto de utilidade
CN107794263B (zh) * 2017-09-30 2019-10-11 河北大学 一种提高黑色素产率的基因及其应用
BR112020023859A2 (pt) * 2018-05-25 2021-04-13 BASF Agricultural Solutions Seed US LLC Plantas, células, partes da planta ou sementes, processos para controle de ervas daninhas, para proteger as plantas, para a produção de uma planta e para aumentar o rendimento e usos da planta
WO2019227036A1 (en) * 2018-05-25 2019-11-28 BASF Agricultural Solutions Seed US LLC Plants containing elite event ee-gm5 and methods and kits for identifying such event in biological samples, and treatment thereof
WO2023040917A1 (zh) * 2021-09-14 2023-03-23 山东舜丰生物科技有限公司 突变的hppd多肽及其应用
WO2023114849A2 (en) * 2021-12-14 2023-06-22 Colorado State University Research Foundation Cyp72a219 genes conferring herbicide tolerance

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CN108884470A (zh) 2018-11-23
ES2933673T3 (es) 2023-02-13
JP6873979B2 (ja) 2021-05-19
EP3347475B1 (en) 2022-11-16
US20180208937A1 (en) 2018-07-26
AU2016319093A1 (en) 2018-03-29
BR112018004779A2 (ru) 2018-10-02
MX2018003044A (es) 2018-04-11
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WO2017042259A1 (en) 2017-03-16
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ZA201802329B (en) 2019-12-18
CA2998036A1 (en) 2017-03-16
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