EA200600050A1 - Способы получения инсулина в растениях - Google Patents

Способы получения инсулина в растениях

Info

Publication number
EA200600050A1
EA200600050A1 EA200600050A EA200600050A EA200600050A1 EA 200600050 A1 EA200600050 A1 EA 200600050A1 EA 200600050 A EA200600050 A EA 200600050A EA 200600050 A EA200600050 A EA 200600050A EA 200600050 A1 EA200600050 A1 EA 200600050A1
Authority
EA
Eurasian Patent Office
Prior art keywords
insulin
nucleic acid
plants
getting
methods
Prior art date
Application number
EA200600050A
Other languages
English (en)
Other versions
EA014887B1 (ru
Inventor
Морис М. Молони
Джозеф Бут
Ричард Кеон
Кори Найкифорук
Гейс Ван Роэйен
Original Assignee
Сембайосиз Джинетикс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Сембайосиз Джинетикс Инк. filed Critical Сембайосиз Джинетикс Инк.
Publication of EA200600050A1 publication Critical patent/EA200600050A1/ru
Publication of EA014887B1 publication Critical patent/EA014887B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Описаны способы получения инсулина в растениях. В одном варианте данное изобретение относится к способу экспрессии инсулина в растениях, включающему: (а) получение конструкции химерной нуклеиновой кислоты, содержащейся в 5'-3' направлении транскрипции в виде функционально связанных компонентов: (i) последовательности нуклеиновой кислоты, способной контролировать экспрессию в клетках семян растений; и (ii) последовательности нуклеиновой кислоты, кодирующей полипептид инсулина; (b) введение конструкции химерной нуклеиновой кислоты в клетку растения; и (с) выращивание клетки растений в зрелом растении, способном давать семена, где семена экспрессируют инсулин.
EA200600050A 2003-06-17 2004-06-17 Способ экспрессии инсулина в семенах растения, способ получения семян растений, содержащих инсулин, и растения, способные производить семена, содержащие инсулин EA014887B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US47881803P 2003-06-17 2003-06-17
US54953904P 2004-03-04 2004-03-04
PCT/CA2004/000896 WO2004111244A2 (en) 2003-06-17 2004-06-17 Methods for the production of insulin in plants

Publications (2)

Publication Number Publication Date
EA200600050A1 true EA200600050A1 (ru) 2006-06-30
EA014887B1 EA014887B1 (ru) 2011-02-28

Family

ID=33555509

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600050A EA014887B1 (ru) 2003-06-17 2004-06-17 Способ экспрессии инсулина в семенах растения, способ получения семян растений, содержащих инсулин, и растения, способные производить семена, содержащие инсулин

Country Status (24)

Country Link
US (2) US7547821B2 (ru)
EP (2) EP1633876B1 (ru)
JP (2) JP4864701B2 (ru)
KR (1) KR101216762B1 (ru)
CN (1) CN1836047B (ru)
AR (1) AR044803A1 (ru)
AT (1) ATE405660T1 (ru)
AU (1) AU2004247762B2 (ru)
BR (1) BRPI0411479A (ru)
CA (1) CA2528741A1 (ru)
CY (1) CY1108477T1 (ru)
DE (1) DE602004015980D1 (ru)
DK (1) DK1633876T3 (ru)
EA (1) EA014887B1 (ru)
ES (1) ES2313018T3 (ru)
HK (1) HK1088037A1 (ru)
IL (1) IL172469A (ru)
MX (1) MXPA05013723A (ru)
NO (1) NO20055885L (ru)
NZ (1) NZ544522A (ru)
PL (1) PL1633876T3 (ru)
PT (1) PT1633876E (ru)
TW (1) TWI345587B (ru)
WO (1) WO2004111244A2 (ru)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003076595A2 (en) * 2002-03-08 2003-09-18 Prodigene, Inc. Production of insulin and insulin-like proteins in plants
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1563075A4 (en) * 2002-11-12 2006-09-06 Ckd Bio Corp HUMANINSULIN EXPRESSIVE PLASMIDE AND METHOD FOR THE PRODUCTION OF HUMANINSULIN THEREWITH
US20040173547A1 (en) * 2003-03-06 2004-09-09 Roth Michael Carl Bolt on attachment to facilitate hanging of pneumatic tool
EA014887B1 (ru) * 2003-06-17 2011-02-28 Сембайосиз Джинетикс Инк. Способ экспрессии инсулина в семенах растения, способ получения семян растений, содержащих инсулин, и растения, способные производить семена, содержащие инсулин
EP1636255A1 (en) * 2003-06-20 2006-03-22 SemBioSys Genetics Inc. Modified oleosins
TW200526778A (en) * 2003-11-14 2005-08-16 Sembiosys Genetics Inc Methods for the production of apolipoproteins in transgenic plants
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
AU2006249983B2 (en) 2005-05-23 2011-03-17 Arcadia Biosciences, Inc. Safflower with elevated gamma-linolenic acid
WO2007053183A2 (en) * 2005-05-27 2007-05-10 University Of Central Florida Chloroplasts engineered to express pharmaceutical proteins
CN101213302B (zh) 2005-07-01 2012-09-05 康乃尔研究基金会 咖啡油质蛋白基因和启动子
WO2007081824A2 (en) * 2006-01-06 2007-07-19 Case Western Reserve University Fibrillation resistant proteins
MX2009002248A (es) 2006-08-31 2009-03-16 Monsanto Technology Llc Metodos para producir plantas transgenicas.
WO2008043033A2 (en) 2006-10-04 2008-04-10 Case Western Reserve University Fibrillation-resistant insulin and insulin analogues
WO2008134461A2 (en) 2007-04-27 2008-11-06 Dow Global Technologies, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2910571B1 (en) 2008-03-18 2016-10-05 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
US8993516B2 (en) * 2008-04-14 2015-03-31 Case Western Reserve University Meal-time insulin analogues of enhanced stability
WO2009132129A2 (en) * 2008-04-22 2009-10-29 Case Western Reserve University Isoform-specific insulin analogues
PL219335B1 (pl) * 2008-07-04 2015-04-30 Inst Biotechnologii I Antybiotyków Pochodna insuliny lub jej farmaceutycznie dopuszczalna sól, jej zastosowanie oraz zawierająca ją kompozycja farmaceutyczna
US9200053B2 (en) 2008-07-31 2015-12-01 Case Western Reserve University Insulin analogues containing penta-fluoro-Phenylalanine at position B24
KR20120129875A (ko) 2008-07-31 2012-11-28 케이스 웨스턴 리저브 유니버시티 염소화 아미노산을 갖는 인슐린 유사체
CN101736029B (zh) * 2008-11-21 2013-01-02 复旦大学 一种利用植物油体蛋白表达系统生产人胰岛素样生长因子-i的方法
US8399407B2 (en) * 2009-09-17 2013-03-19 Case Western Reserve University Non-standard insulin analogues
RU2012123642A (ru) 2009-12-11 2014-01-20 Кейз Вестерн Ризев Юнивесити Аналоги инсулина, содержащие хлорированные аминокислоты
CN102268451B (zh) * 2010-06-01 2014-03-12 安胜军 一种含有人胰岛素基因的表达载体及其构建方法与应用
CN103154022A (zh) 2010-06-16 2013-06-12 富途锐基尼以色列有限公司 抗害虫植物
US20130164288A1 (en) 2010-09-07 2013-06-27 Protalix Ltd. Readthrough acetylcholinesterase (ache-r) for treating or preventing parkinson's disease
EP2768520A4 (en) * 2011-10-18 2015-07-15 AmideBio LLC CHEMICALLY AND THERMODYNAMICALLY STABLE INSULIN ANALOGUES AND IMPROVED PRODUCTION METHODS THEREFOR
US9352456B2 (en) 2011-10-26 2016-05-31 Black & Decker Inc. Power tool with force sensing electronic clutch
CN104364260B (zh) 2012-04-11 2017-02-22 诺和诺德股份有限公司 胰岛素制剂
WO2014172488A2 (en) 2013-04-17 2014-10-23 AmideBio LLC Chemically and thermodynamically stable insulin analogues and improved methods for their production
KR101625746B1 (ko) 2013-09-23 2016-05-31 순천대학교 산학협력단 프로미니인슐린 재조합 단백질이 발현된 형질전환 식물 및 이의 제조방법
KR102569743B1 (ko) 2014-10-06 2023-08-23 케이스 웨스턴 리저브 유니버시티 이상 단일 사슬 인슐린 유사체
MA49116A (fr) 2016-12-16 2020-03-25 Novo Nordisk As Compositions pharmaceutiques contenant de l'insuline
KR102642178B1 (ko) * 2017-08-08 2024-02-29 중앙대학교 산학협력단 돌연변이에 의해, 혈중 반감기 및 공수병 바이러스 중화 효능이 증대된 공수병 바이러스 중화 항체
WO2019154311A1 (zh) * 2018-02-09 2019-08-15 江苏恒瑞医药股份有限公司 一种密码子优化的人胰岛素类似物前体基因和信号肽基因
CN114144426A (zh) * 2019-04-19 2022-03-04 印地安纳大学理事会 餐时或基础胰岛素类似物通过内部二硒桥的稳定化
CA3159054A1 (en) * 2019-12-06 2021-06-10 Mary S. MORRIS Methods and compositions for the treatment and prevention of type 1 diabetes
AU2021300450A1 (en) * 2020-07-03 2023-03-02 Kriya Therapeutics, Inc. Modified insulin and glucokinase nucleic acids for treating diabetes
EP4190905A1 (en) 2021-12-01 2023-06-07 Core Biogenesis Method for the purification of recombinant proteins using oil body proteins

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3971856A (en) 1975-03-03 1976-07-27 Archer Daniels Midland Company Process for preparing soy protein concentrate
US6174724B1 (en) 1983-01-17 2001-01-16 Monsanto Company Chimeric genes suitable for expression in plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
DK58285D0 (da) 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US5008241A (en) 1985-03-12 1991-04-16 Novo Nordisk A/S Novel insulin peptides
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
EP0218571B1 (en) 1985-08-07 1993-02-03 Monsanto Company Glyphosate-resistant plants
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
ES2121803T3 (es) 1987-05-20 1998-12-16 Novartis Ag Plantas de zea mays y plantas transgenicas de zea mays generadas a partir de protoplastos o celulas derivadas de protoplastos.
DK336188D0 (da) 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
CA1341467C (en) 1988-07-29 2004-12-07 John C. Rogers Producing commercially valuable polypeptides with genetically transformed endosperm tissue
JPH04502465A (ja) 1988-12-23 1992-05-07 ノボ　ノルディスク　アクティーゼルスカブ ヒトインシュリン類似物質
DE69034081T2 (de) 1989-03-24 2004-02-12 Syngenta Participations Ag Krankheitsresistente transgene Pflanze
NL8901932A (nl) 1989-07-26 1991-02-18 Mogen Int Produktie van heterologe eiwitten in planten of plantecellen.
US4940935A (en) 1989-08-28 1990-07-10 Ried Ashman Manufacturing Automatic SMD tester
US5202422A (en) * 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5959177A (en) * 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
KR910014790A (ko) 1990-01-10 1991-08-31 한태희 랩탑 컴퓨터의 기능선택방법 및 인터페이스회로
JPH05505525A (ja) * 1990-03-05 1993-08-19 ジ・アップジョン・カンパニー 種子特異的調節配列類を介する蛋白発現
US5650554A (en) 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
UA48104C2 (ru) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, содержащий последовательность, которая кодирует инсектицидный протеин, оптимизированную для кукурузы, фрагмент днк, обеспечивающий направленную желательную для сердцевины стебля экспрессию связанного с ней структурного гена в растении, фрагмент днк, обеспечивающий специфическую для пыльцы экспрессию связанного с ней структурного гена в растении, рекомбинантная молекула днк, способ получения оптимизированной для кукурузы кодирующей последовательности инсектицидного протеина, способ защиты растений кукурузы по меньшей мере от одного насекомого-вредителя
UA41319C2 (ru) 1992-04-02 2001-09-17 Сембайозіс Дженетікс Інк. Способ экспрессии последовательности днк, представляющей интерес, в клетке семян, химерный ген, экспрессирующая кассета, изолированный регуляторный участок транскрипции, способ изменения специфичного для семян метаболизма, способ получения новых полипептидов в семенах, изолированная днк, способ экспресии последовательности днк, представляющей интерес, у растения-хозяина, способ получения очищенного полипептида, представляющего интерес, способ получения полипептида, представляющего интерес, в масляном теле
AU5843794A (en) * 1992-12-28 1994-07-19 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
US5461031A (en) 1994-06-16 1995-10-24 Eli Lilly And Company Monomeric insulin analog formulations
US5474978A (en) 1994-06-16 1995-12-12 Eli Lilly And Company Insulin analog formulations
US5633437A (en) 1994-10-11 1997-05-27 Sandoz Ltd. Gene exhibiting resistance to acetolactate synthase inhibitor herbicides
US6040496A (en) 1995-06-30 2000-03-21 Novartis Finance Corporation Use of translationally altered RNA to confer resistance to maize dwarf mosaic virus and other monocotyledonous plant viruses
US5577474A (en) * 1995-11-29 1996-11-26 General Motors Corporation Torque estimation for engine speed control
WO1997029200A1 (de) * 1996-02-08 1997-08-14 Institut für Pflanzengenetik und Kulturpflanzenforschung Kassetten zur expression von lagerstabilen proteinen in pflanzen
US5856452A (en) * 1996-12-16 1999-01-05 Sembiosys Genetics Inc. Oil bodies and associated proteins as affinity matrices
AU742794B2 (en) 1997-04-25 2002-01-10 Sembiosys Genetics Inc. Method for cleavage of fusion proteins
ES2276475T5 (es) * 1997-09-30 2014-07-11 The Regents Of The University Of California Producción de proteínas en semillas de plantas
AU3868199A (en) 1998-04-27 1999-11-16 Demegen, Inc. A method for increasing the protein content of plants
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
ES2261226T3 (es) 1999-08-27 2006-11-16 Sembiosys Genetics Inc. Promotores especificos de la semilla de lino.
US6521738B2 (en) 1999-12-29 2003-02-18 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
EP1274846A4 (en) * 2000-03-01 2004-09-29 Univ Auburn PLASTIDE TRANSFORMATION VECTOR FOR THE EXPRESSION OF HUMAN PROTEINS IN PLANTS
CN1295129A (zh) * 2000-08-25 2001-05-16 林忠平 利用转基因番茄生产人胰岛素的方法
BR0116220A (pt) * 2000-12-19 2003-09-23 Sembiosys Genetics Inc Métodos para a produção de proteìnas multiméricas e composições relacionadas
US6841979B2 (en) 2001-05-22 2005-01-11 Powerdsine, Ltd. Power distribution with digital current control
WO2003076595A2 (en) 2002-03-08 2003-09-18 Prodigene, Inc. Production of insulin and insulin-like proteins in plants
CN1382734A (zh) * 2002-03-15 2002-12-04 林忠平 利用转基因生菜生产人胰岛素的方法
EA014887B1 (ru) * 2003-06-17 2011-02-28 Сембайосиз Джинетикс Инк. Способ экспрессии инсулина в семенах растения, способ получения семян растений, содержащих инсулин, и растения, способные производить семена, содержащие инсулин

Also Published As

Publication number Publication date
EP1959017A1 (en) 2008-08-20
AU2004247762A1 (en) 2004-12-23
TW200510529A (en) 2005-03-16
JP2011200251A (ja) 2011-10-13
US20050039235A1 (en) 2005-02-17
IL172469A0 (en) 2006-04-10
IL172469A (en) 2011-08-31
TWI345587B (en) 2011-07-21
PT1633876E (pt) 2008-11-03
CN1836047A (zh) 2006-09-20
EP1633876B1 (en) 2008-08-20
ES2313018T3 (es) 2009-03-01
WO2004111244A2 (en) 2004-12-23
KR20060052705A (ko) 2006-05-19
US7547821B2 (en) 2009-06-16
JP2007523611A (ja) 2007-08-23
NZ544522A (en) 2008-03-28
EP1633876A2 (en) 2006-03-15
CA2528741A1 (en) 2004-12-23
DK1633876T3 (da) 2008-12-08
EA014887B1 (ru) 2011-02-28
AU2004247762B2 (en) 2010-05-27
PL1633876T3 (pl) 2009-01-30
CY1108477T1 (el) 2014-04-09
CN1836047B (zh) 2010-12-22
JP4864701B2 (ja) 2012-02-01
AR044803A1 (es) 2005-10-05
NO20055885L (no) 2006-01-17
KR101216762B1 (ko) 2013-01-02
BRPI0411479A (pt) 2006-07-25
US20090282583A1 (en) 2009-11-12
HK1088037A1 (en) 2006-10-27
WO2004111244A3 (en) 2005-02-03
MXPA05013723A (es) 2006-03-13
DE602004015980D1 (de) 2008-10-02
ATE405660T1 (de) 2008-09-15

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