CN102268451B - 一种含有人胰岛素基因的表达载体及其构建方法与应用 - Google Patents
一种含有人胰岛素基因的表达载体及其构建方法与应用 Download PDFInfo
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- CN102268451B CN102268451B CN201110142310.3A CN201110142310A CN102268451B CN 102268451 B CN102268451 B CN 102268451B CN 201110142310 A CN201110142310 A CN 201110142310A CN 102268451 B CN102268451 B CN 102268451B
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- oil body
- insulin
- gene
- expression vector
- plant
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (12)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110142310.3A CN102268451B (zh) | 2010-06-01 | 2011-05-30 | 一种含有人胰岛素基因的表达载体及其构建方法与应用 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010188296.6 | 2010-06-01 | ||
CN201010188296 | 2010-06-01 | ||
CN201110142310.3A CN102268451B (zh) | 2010-06-01 | 2011-05-30 | 一种含有人胰岛素基因的表达载体及其构建方法与应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102268451A CN102268451A (zh) | 2011-12-07 |
CN102268451B true CN102268451B (zh) | 2014-03-12 |
Family
ID=45050911
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201110142310.3A Expired - Fee Related CN102268451B (zh) | 2010-06-01 | 2011-05-30 | 一种含有人胰岛素基因的表达载体及其构建方法与应用 |
Country Status (2)
Country | Link |
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CN (1) | CN102268451B (zh) |
WO (1) | WO2011150841A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104017809A (zh) * | 2014-06-05 | 2014-09-03 | 山东省农业科学院生物技术研究中心 | 改造的人Insulin蛋白表达基因及表达方法 |
CN106701784B (zh) * | 2017-01-24 | 2020-04-17 | 河南农业大学 | 大豆油体蛋白基因GmOLEO1及其编码蛋白与应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1836047A (zh) * | 2003-06-17 | 2006-09-20 | 赛姆生物系统遗传公司 | 在植物中生产胰岛素的方法 |
CN101037692A (zh) * | 2006-12-28 | 2007-09-19 | 吉林农业大学 | 利用植物油体表达人胰岛素的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1274830C (zh) * | 2001-12-14 | 2006-09-13 | 中国农业科学院生物技术研究所 | 一种新型鲑鱼降钙素类似物及其在植物油体中表达的方法 |
CN101736029B (zh) * | 2008-11-21 | 2013-01-02 | 复旦大学 | 一种利用植物油体蛋白表达系统生产人胰岛素样生长因子-i的方法 |
CN101586117A (zh) * | 2009-03-20 | 2009-11-25 | 中山大学 | 花生油体蛋白基因启动子在驱动外源基因在转基因植物种子中特异表达上的应用 |
CN101591671B (zh) * | 2009-06-30 | 2010-12-08 | 山东省农业科学院高新技术研究中心 | 一种在花生种子中表达人成骨蛋白-1的方法 |
-
2011
- 2011-05-30 CN CN201110142310.3A patent/CN102268451B/zh not_active Expired - Fee Related
- 2011-05-31 WO PCT/CN2011/075039 patent/WO2011150841A1/zh active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1836047A (zh) * | 2003-06-17 | 2006-09-20 | 赛姆生物系统遗传公司 | 在植物中生产胰岛素的方法 |
CN101037692A (zh) * | 2006-12-28 | 2007-09-19 | 吉林农业大学 | 利用植物油体表达人胰岛素的方法 |
Non-Patent Citations (1)
Title |
---|
kjeldsen,et al..Expression of insulin in yeast:the importance of molecular adaptation for secretion and conversion.《BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS》.2001,第18卷全文. * |
Also Published As
Publication number | Publication date |
---|---|
WO2011150841A1 (zh) | 2011-12-08 |
CN102268451A (zh) | 2011-12-07 |
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Effective date of registration: 20170120 Address after: 050000 Hebei province high tech Zone, Yangtze River Road, No., run the headquarters of the international building, room 2, No. 501, room 319 Patentee after: Hebei new Xinuo Biological Medicine Technology Co Ltd Address before: 050026 Hebei Province, Shijiazhuang city Yuhua District Road No. 88 Fangxing Patentee before: An Shengjun Patentee before: Chai Xiqing Patentee before: Wang Kunsheng |
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Effective date of registration: 20210114 Address after: 050026 No.88, Fangxing Road, Yuhua District, Shijiazhuang City, Hebei Province Patentee after: HEBEI CHEMICAL & PHARMACEUTICAL College Address before: 050000 Room 501, International Building 2, Runjiang headquarters, 319 Changjiang Avenue, high tech Zone, Shijiazhuang City, Hebei Province Patentee before: Hebei new Xinuo Biological Medicine Technology Co.,Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20140312 Termination date: 20210530 |