CN102127562B - 一种种子特异型表达载体及其构建方法与应用 - Google Patents
一种种子特异型表达载体及其构建方法与应用 Download PDFInfo
- Publication number
- CN102127562B CN102127562B CN2010105800170A CN201010580017A CN102127562B CN 102127562 B CN102127562 B CN 102127562B CN 2010105800170 A CN2010105800170 A CN 2010105800170A CN 201010580017 A CN201010580017 A CN 201010580017A CN 102127562 B CN102127562 B CN 102127562B
- Authority
- CN
- China
- Prior art keywords
- apolipoproteins
- gene
- oil body
- milano variant
- expression vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (13)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2010105800170A CN102127562B (zh) | 2009-12-09 | 2010-12-09 | 一种种子特异型表达载体及其构建方法与应用 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200910250703.9 | 2009-12-09 | ||
CN200910250703 | 2009-12-09 | ||
CN2010105800170A CN102127562B (zh) | 2009-12-09 | 2010-12-09 | 一种种子特异型表达载体及其构建方法与应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102127562A CN102127562A (zh) | 2011-07-20 |
CN102127562B true CN102127562B (zh) | 2013-01-30 |
Family
ID=44145108
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2010105800170A Expired - Fee Related CN102127562B (zh) | 2009-12-09 | 2010-12-09 | 一种种子特异型表达载体及其构建方法与应用 |
Country Status (3)
Country | Link |
---|---|
US (1) | US9745362B2 (zh) |
CN (1) | CN102127562B (zh) |
WO (1) | WO2011069459A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102925480B (zh) * | 2012-11-02 | 2014-05-07 | 浙江大学 | 一种抑制水稻2-pgk基因表达的植物表达载体及培育低植酸水稻的方法 |
CN104232654A (zh) * | 2013-06-07 | 2014-12-24 | 中国科学院植物研究所 | 一种表达盒及其在培育种子油脂含量增高的转基因植物中的应用 |
CN109706150B (zh) * | 2019-01-24 | 2020-07-28 | 山东省花生研究所(山东省农业科学院花生工程技术研究中心) | 一种花生种子特异表达启动子ahssp29及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101586117A (zh) * | 2009-03-20 | 2009-11-25 | 中山大学 | 花生油体蛋白基因启动子在驱动外源基因在转基因植物种子中特异表达上的应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6288304B1 (en) * | 1991-02-22 | 2001-09-11 | Sembiosys Genetics Inc. | Expression of somatotropin in plant seeds |
WO1993010241A1 (en) * | 1991-11-20 | 1993-05-27 | Calgene, Inc. | FATTY ACYL-CoA: FATTY ALCOHOL O-ACYLTRANSFERASES |
US5643757A (en) * | 1994-03-21 | 1997-07-01 | American Cyanamid Company | High yield production of human apolipoprotein A1 in E. coli. |
GB2332908B (en) | 1996-10-31 | 2001-03-28 | Ca Nat Research Council | Flax promoter for manipulating gene expression |
AU782218B2 (en) | 1999-08-27 | 2005-07-14 | Commonwealth Scientific And Industrial Research Organisation | Flax seed specific promoters |
NZ526822A (en) | 2000-12-19 | 2005-03-24 | Sembiosys Genetics Inc | Methods for the production of multimeric proteins for producing an oil body associated with a recombinant protein complex preferably thioredoxin or an immunoglobin, in transgenic plants and their use in compositons for reducing allergenicity of food |
US20060179514A1 (en) * | 2001-07-05 | 2006-08-10 | Sembiosys Genetics, Inc. | Methods for the production of multimeric protein complexes, and related compositions |
TW200526778A (en) * | 2003-11-14 | 2005-08-16 | Sembiosys Genetics Inc | Methods for the production of apolipoproteins in transgenic plants |
KR20090018799A (ko) * | 2006-05-30 | 2009-02-23 | 다우 글로벌 테크놀로지스 인크. | 코돈 최적화 방법 |
EA021282B1 (ru) | 2006-08-10 | 2015-05-29 | ПЛАНТЕКНО С.р.л. | ДИМЕРНЫЕ И/ИЛИ ОЛИГОМЕРНЫЕ ФОРМЫ МУТЕИНА БЕЛКА ApoA-1 ЧЕЛОВЕКА, ПОЛУЧЕННЫЕ В СЕМЕНАХ РАСТЕНИЙ, И ИХ ПРИМЕНЕНИЕ |
CN101037692A (zh) | 2006-12-28 | 2007-09-19 | 吉林农业大学 | 利用植物油体表达人胰岛素的方法 |
-
2010
- 2010-12-09 US US13/515,209 patent/US9745362B2/en active Active
- 2010-12-09 WO PCT/CN2010/079620 patent/WO2011069459A1/zh active Application Filing
- 2010-12-09 CN CN2010105800170A patent/CN102127562B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101586117A (zh) * | 2009-03-20 | 2009-11-25 | 中山大学 | 花生油体蛋白基因启动子在驱动外源基因在转基因植物种子中特异表达上的应用 |
Non-Patent Citations (2)
Title |
---|
李小东.花生种子特异表达载体构建与农杆菌介导基因转化技术体系的优化.《山东农业大学博士论文》.2009,全文. * |
赵传志.以油体作为生物反应器的研究进展.《生物技术通报》.2008,(第2 期),p73-76,79. * |
Also Published As
Publication number | Publication date |
---|---|
CN102127562A (zh) | 2011-07-20 |
US20120288893A1 (en) | 2012-11-15 |
WO2011069459A1 (zh) | 2011-06-16 |
US9745362B2 (en) | 2017-08-29 |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170112 Address after: 050035 Hebei province high tech Zone, Yangtze River Road, No., run the headquarters of the international building, room 2, No. 501, room 319 Patentee after: Hebei new Xinuo Biological Medicine Technology Co Ltd Address before: 050026 Hebei Province, Shijiazhuang city Yuhua District Road No. 88 Fangxing Patentee before: An Shengjun Patentee before: Chai Xiqing Patentee before: Wang Kunsheng |
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TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210106 Address after: 050026 No.88, Fangxing Road, Yuhua District, Shijiazhuang City, Hebei Province Patentee after: HEBEI CHEMICAL & PHARMACEUTICAL College Address before: 050035 Room 501, International Building 2, Runjiang headquarters, 319 Changjiang Avenue, high tech Zone, Shijiazhuang City, Hebei Province Patentee before: Hebei new Xinuo Biological Medicine Technology Co.,Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20201209 |