EA009287B1 - Получение l-аскорбиновой кислоты в результате микробиологического процесса - Google Patents
Получение l-аскорбиновой кислоты в результате микробиологического процесса Download PDFInfo
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- EA009287B1 EA009287B1 EA200600408A EA200600408A EA009287B1 EA 009287 B1 EA009287 B1 EA 009287B1 EA 200600408 A EA200600408 A EA 200600408A EA 200600408 A EA200600408 A EA 200600408A EA 009287 B1 EA009287 B1 EA 009287B1
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- polynucleotide
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- microorganism
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- ascorbic acid
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
-
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03017677 | 2003-08-14 | ||
| PCT/CH2004/000511 WO2005017159A2 (en) | 2003-08-14 | 2004-08-16 | Microbial production of l-ascorbic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200600408A1 EA200600408A1 (ru) | 2006-08-25 |
| EA009287B1 true EA009287B1 (ru) | 2007-12-28 |
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| EA200600408A EA009287B1 (ru) | 2003-08-14 | 2004-08-16 | Получение l-аскорбиновой кислоты в результате микробиологического процесса |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US7700723B2 (enExample) |
| EP (2) | EP1664303B1 (enExample) |
| JP (1) | JP4896718B2 (enExample) |
| KR (1) | KR101311562B1 (enExample) |
| CN (2) | CN104004776A (enExample) |
| BR (1) | BRPI0413542A (enExample) |
| EA (1) | EA009287B1 (enExample) |
| ES (2) | ES2593811T3 (enExample) |
| WO (2) | WO2005017159A2 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101151365B (zh) * | 2005-02-11 | 2012-05-23 | 帝斯曼知识产权资产管理有限公司 | 基因sms02 |
| WO2006084733A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Gene rcs 21 |
| WO2006084643A2 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene vcs 07 |
| WO2006084645A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene rcs 10 |
| WO2006084737A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Gene rcs 23 |
| WO2006084735A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Gene for coenzyme pqq synthesis protein b from gluconobacter oxydans |
| US20080193972A1 (en) * | 2005-02-11 | 2008-08-14 | Bastian Chevreux | Gene Rcs 25 |
| EP1846554A1 (en) * | 2005-02-11 | 2007-10-24 | DSMIP Assets B.V. | Fermentive vitamin c production |
| WO2006084724A1 (en) * | 2005-02-11 | 2006-08-17 | Dsm Ip Assets B.V. | Novel gene rcs 28 |
| WO2007028602A1 (en) | 2005-09-08 | 2007-03-15 | Dsm Ip Assets B.V. | Novel gene sms 37 |
| EP1931785A2 (en) * | 2005-09-09 | 2008-06-18 | DSMIP Assets B.V. | Novel gene gms 01 |
| US8137940B2 (en) | 2005-09-09 | 2012-03-20 | Dsm Ip Assets B.V. | Method for production of biomass using a gluconobacter oxydans comprising an inactivated NADP dependent glucose dehydrogenase gene |
| FR2906817B1 (fr) * | 2006-10-04 | 2012-04-06 | Gervais Danone Sa | Procede de culture pour favoriser la production de vitamine k2 par les bacteries lactiques et ses applications a la preparation de produits alimentaires |
| EP1918383A1 (en) * | 2006-11-02 | 2008-05-07 | DSMIP Assets B.V. | Semi-continuous fermentation process for pantothenate production |
| EP1918382A1 (en) * | 2006-11-02 | 2008-05-07 | DSMIP Assets B.V. | Semi-continuous cascade fermentation process for pantothenate production |
| US20100323412A1 (en) * | 2008-01-10 | 2010-12-23 | Bastien Chevreux | Novel gene sms 44 |
| CN103073601B (zh) * | 2013-02-01 | 2015-08-19 | 东北制药集团股份有限公司 | 一种2-酮基-l-古龙酸溶液加晶种的可控浓缩结晶方法 |
| NZ743055A (en) * | 2013-03-08 | 2020-03-27 | Xyleco Inc | Equipment protecting enclosures |
| CN105392892A (zh) | 2013-03-27 | 2016-03-09 | 六品科技公司 | 重组噬菌体和细菌检测方法 |
| JP2016521996A (ja) * | 2013-06-19 | 2016-07-28 | サンプルシックス テクノロジーズ，インコーポレイティド | ファージベースの細菌検出アッセイ |
| CN105683210B (zh) | 2013-08-23 | 2020-07-14 | 贝林格尔·英格海姆Rcv两合公司 | 用于细胞破碎和/或回收生物分子的微粒 |
| EP3122870B1 (en) * | 2014-03-25 | 2022-06-29 | Ginkgo Bioworks Inc. | Methods and genetic systems for cell engineering |
| CN116053017B (zh) * | 2022-11-04 | 2023-08-22 | 医波(厦门)科技有限公司 | 一种复合磁性微球及其制备方法和应用 |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997004101A2 (de) * | 1995-07-17 | 1997-02-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Enzym geeignet zur oxidation von cyclitolen und ihren derivaten |
| EP0911415A2 (en) * | 1990-09-17 | 1999-04-28 | F. Hoffmann-La Roche AG | Microbiological manufacture of L-ascorbic acid |
| EP0922759A2 (en) * | 1997-12-01 | 1999-06-16 | F. Hoffmann-La Roche Ag | Aldehyde dehydrogenase |
| WO2003016508A2 (en) * | 2001-08-15 | 2003-02-27 | Cerestar Holding B.V. | Process for the manufacture of 2-keto-l-gulonic acid |
| WO2003089634A1 (en) * | 2002-04-22 | 2003-10-30 | Dsm Ip Assets B.V. | Aldehyde dehydrogenase ii |
| WO2003104445A1 (en) * | 2002-06-06 | 2003-12-18 | Roche Vitamins Ag | Aldehyde dehydrogenase |
| WO2004029269A1 (en) * | 2002-09-27 | 2004-04-08 | Dsm Ip Assets B.V. | Process for producing vitamin c |
| WO2004029235A2 (en) * | 2002-09-27 | 2004-04-08 | Dsm Ip Assets B.V. | Aldehyde dehydrogenase gene |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5082785A (en) | 1987-01-30 | 1992-01-21 | Hoffmann-La Roche Inc. | Biosynthesis of 2 keto-l-gulonic acid |
| WO1989006688A2 (en) | 1988-01-14 | 1989-07-27 | F. Hoffmann-La Roche & Co. Aktiengesellschaft | Novel enzyme |
| US5376544A (en) * | 1992-09-08 | 1994-12-27 | Rutgers The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| US20020012979A1 (en) * | 1998-06-08 | 2002-01-31 | Alan Berry | Vitamin c production in microorganisms and plants |
| CN100357446C (zh) * | 2002-09-27 | 2007-12-26 | 帝斯曼知识产权资产管理有限公司 | 维生素c的微生物生产方法 |
| CN1914326B (zh) * | 2004-01-30 | 2012-04-18 | 帝斯曼知识产权资产管理有限公司 | 维生素c的微生物生产方法 |
| US20080226126A1 (en) * | 2005-01-31 | 2008-09-18 | Yoshinori Ohno | Object-Tracking Apparatus, Microscope System, and Object-Tracking Program |
| EP1846554A1 (en) * | 2005-02-11 | 2007-10-24 | DSMIP Assets B.V. | Fermentive vitamin c production |
-
2004
- 2004-08-16 EA EA200600408A patent/EA009287B1/ru not_active IP Right Cessation
- 2004-08-16 ES ES10184019.7T patent/ES2593811T3/es not_active Expired - Lifetime
- 2004-08-16 CN CN201410171433.3A patent/CN104004776A/zh active Pending
- 2004-08-16 ES ES04738146T patent/ES2421294T3/es not_active Expired - Lifetime
- 2004-08-16 JP JP2006522868A patent/JP4896718B2/ja not_active Expired - Fee Related
- 2004-08-16 EP EP04738146.2A patent/EP1664303B1/en not_active Expired - Lifetime
- 2004-08-16 EP EP10184019.7A patent/EP2348113B1/en not_active Expired - Lifetime
- 2004-08-16 US US10/567,763 patent/US7700723B2/en not_active Expired - Lifetime
- 2004-08-16 CN CN200480023361.2A patent/CN1882691B/zh not_active Expired - Fee Related
- 2004-08-16 WO PCT/CH2004/000511 patent/WO2005017159A2/en not_active Ceased
- 2004-08-16 KR KR1020067003014A patent/KR101311562B1/ko not_active Expired - Fee Related
- 2004-08-16 WO PCT/CH2004/000512 patent/WO2005017172A1/en not_active Ceased
- 2004-08-16 BR BRPI0413542-3A patent/BRPI0413542A/pt not_active Application Discontinuation
-
2010
- 2010-02-08 US US12/701,748 patent/US8338144B2/en not_active Expired - Fee Related
-
2012
- 2012-11-21 US US13/683,487 patent/US20130217080A1/en not_active Abandoned
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0911415A2 (en) * | 1990-09-17 | 1999-04-28 | F. Hoffmann-La Roche AG | Microbiological manufacture of L-ascorbic acid |
| WO1997004101A2 (de) * | 1995-07-17 | 1997-02-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Enzym geeignet zur oxidation von cyclitolen und ihren derivaten |
| EP0922759A2 (en) * | 1997-12-01 | 1999-06-16 | F. Hoffmann-La Roche Ag | Aldehyde dehydrogenase |
| WO2003016508A2 (en) * | 2001-08-15 | 2003-02-27 | Cerestar Holding B.V. | Process for the manufacture of 2-keto-l-gulonic acid |
| WO2003089634A1 (en) * | 2002-04-22 | 2003-10-30 | Dsm Ip Assets B.V. | Aldehyde dehydrogenase ii |
| WO2003104445A1 (en) * | 2002-06-06 | 2003-12-18 | Roche Vitamins Ag | Aldehyde dehydrogenase |
| WO2004029269A1 (en) * | 2002-09-27 | 2004-04-08 | Dsm Ip Assets B.V. | Process for producing vitamin c |
| WO2004029235A2 (en) * | 2002-09-27 | 2004-04-08 | Dsm Ip Assets B.V. | Aldehyde dehydrogenase gene |
Non-Patent Citations (7)
| Title |
|---|
| DATABASE EMBL [Online], 18 December 2001 (2001-12-18), "Agrobacterium tumefaciens str. C58 linear chromosome, section 35 of 187 of the complete sequence", XP002321379, retrieved from EBI accession no. EM_PRO:AE009265, Database accession no. AE009265, the whole document * |
| DATABASE UniProt [Online], 1 June 2003 (2003-06-01), "Glucose dehydrogenase", XP002321380, retrieved from EBI accession no. UNIPROT:Q882Q7, Database accession no. Q882Q7, the whole document * |
| LEE H-W ET AL: "Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 23, no. 2, August 1999 (1999-08), pages 106-111, XP002241676, ISSN: 1367-5435, the whole document * |
| SAITO Y ET AL: "CLONING OF GENES CODING FOR L-SORBOSE AND L-SORBOSONE DEHYDROGENASES FROM GLUCONOBACTER OXYDANS AND MICROBIAL PRODUCTION OF 2-KETO-L-GULONATE, A PRECURSOR OF L-ASCORBIC ACID, IN A RECOMBINANT G. OXYDANS STRAIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 63, no. 2, 1997, pages 454-460, XP000886144, ISSN: 0099-2240, the whole document * |
| SHINJOH M ET AL: "Cloning and Nucleotide Sequencing of the Membrane-Bound L-Sorbosone Dehydrogenase Gene of Acetobacter liquefaciens IFO 12258, and Its Expression in Gluconobacter oxydans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 61, no. 2, February 1995 (1995-02), pages 413-420, XP002267229, ISSN: 0099-2240, table 1 * |
| SUGISAWA T ET AL: "ISOLATION AND CHARACTERIZATION OF A NEW VITAMIN C PRODUCING ENZYME (L-GULONO-GAMMA-LACTONE DEHYDROGENASE) OF BACTERIAL ORIGIN", BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY, XX, XX, vol. 59, no. 2, February 1995 (1995-02), pages 190-196, XP001084987, ISSN: 0916-8451, page 191-page 192 * |
| SUGISAWA T ET AL: "MICROBIAL PRODUCTION OF 2-KETO-L-GULONIC ACID FROM L-SORBOSE AND D-SORBITOL BY GLUCONOBACTER MELANOGENUS", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 54, no. 5, May 1990 (1990-05), pages 1201-1209, XP002061325, ISSN: 0002-1369, cited in the application, table 3 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1882691A (zh) | 2006-12-20 |
| US20130217080A1 (en) | 2013-08-22 |
| ES2421294T3 (es) | 2013-08-30 |
| EP2348113A3 (en) | 2012-11-14 |
| JP4896718B2 (ja) | 2012-03-14 |
| US20100203598A1 (en) | 2010-08-12 |
| ES2593811T3 (es) | 2016-12-13 |
| WO2005017159A2 (en) | 2005-02-24 |
| CN104004776A (zh) | 2014-08-27 |
| EA200600408A1 (ru) | 2006-08-25 |
| US8338144B2 (en) | 2012-12-25 |
| KR101311562B1 (ko) | 2013-09-26 |
| US7700723B2 (en) | 2010-04-20 |
| EP2348113B1 (en) | 2016-06-29 |
| EP2348113A2 (en) | 2011-07-27 |
| JP2007502102A (ja) | 2007-02-08 |
| EP1664303B1 (en) | 2013-04-24 |
| WO2005017159A3 (en) | 2005-11-03 |
| BRPI0413542A (pt) | 2006-10-17 |
| US20070184534A1 (en) | 2007-08-09 |
| KR20060064629A (ko) | 2006-06-13 |
| EP1664303A2 (en) | 2006-06-07 |
| WO2005017172A1 (en) | 2005-02-24 |
| CN1882691B (zh) | 2014-05-28 |
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