DK3456821T3 - Ikke-integrerende dna-vektorer til genetisk modifikation af celler - Google Patents
Ikke-integrerende dna-vektorer til genetisk modifikation af celler Download PDFInfo
- Publication number
- DK3456821T3 DK3456821T3 DK17191829.5T DK17191829T DK3456821T3 DK 3456821 T3 DK3456821 T3 DK 3456821T3 DK 17191829 T DK17191829 T DK 17191829T DK 3456821 T3 DK3456821 T3 DK 3456821T3
- Authority
- DK
- Denmark
- Prior art keywords
- cells
- genetic modification
- dna vectors
- integrating dna
- integrating
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17191829.5A EP3456821B2 (en) | 2017-09-19 | 2017-09-19 | Non-integrating dna vectors for the genetic modification of cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3456821T3 true DK3456821T3 (da) | 2020-11-16 |
| DK3456821T4 DK3456821T4 (da) | 2024-04-15 |
Family
ID=60037350
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17191829.5T DK3456821T4 (da) | 2017-09-19 | 2017-09-19 | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| DK18769378.3T DK3684925T3 (da) | 2017-09-19 | 2018-09-19 | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| DK18769377.5T DK3684924T3 (da) | 2017-09-19 | 2018-09-19 | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK18769378.3T DK3684925T3 (da) | 2017-09-19 | 2018-09-19 | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| DK18769377.5T DK3684924T3 (da) | 2017-09-19 | 2018-09-19 | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US20200277624A1 (da) |
| EP (4) | EP3456821B2 (da) |
| JP (3) | JP7189943B2 (da) |
| KR (2) | KR20200054278A (da) |
| CN (2) | CN111492056A (da) |
| AU (2) | AU2018337672A1 (da) |
| CA (2) | CA3017658A1 (da) |
| DK (3) | DK3456821T4 (da) |
| ES (3) | ES2821655T3 (da) |
| FI (2) | FI3456821T4 (da) |
| HU (1) | HUE060893T2 (da) |
| IL (2) | IL273267A (da) |
| LT (1) | LT3684924T (da) |
| MX (1) | MX2020003092A (da) |
| PL (1) | PL3684924T3 (da) |
| PT (1) | PT3684924T (da) |
| SG (2) | SG11202001941SA (da) |
| WO (2) | WO2019060253A1 (da) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| EP3092049A1 (en) | 2014-01-08 | 2016-11-16 | Flodesign Sonics Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| BR112020009889A2 (pt) | 2017-12-14 | 2020-11-03 | Flodesign Sonics, Inc. | acionador e controlador de transdutor acústico |
| CA3132420A1 (en) * | 2019-03-18 | 2020-09-24 | Deutsches Krebsforschungszentrum | Expression constructs for the genetic modification of cells |
| TW202124722A (zh) | 2019-09-18 | 2021-07-01 | 美商英特佳樂帝克醫療公司 | 合成dna載體及其使用方法 |
| JP2023537858A (ja) | 2020-07-22 | 2023-09-06 | ドイチェス クレブスフォルシュンクスツェントルム | ５’ｓ／ｍａｒの適用 |
| CN113025651B (zh) * | 2021-03-31 | 2023-03-24 | 重庆医科大学 | 靶向HBV核心启动子的药物筛选细胞模型、Triciribine及结构类似物新应用 |
| WO2022272297A1 (en) | 2021-06-25 | 2022-12-29 | Oxford Biomedica Solutions Llc | Adeno-associated virus packaging systems |
| WO2023083982A1 (en) | 2021-11-11 | 2023-05-19 | Universitaet Heidelberg | Immunoreactive molecules and uses thereof |
| WO2023083985A1 (en) | 2021-11-11 | 2023-05-19 | Patrick Schmidt | Immunoreactive molecules and uses thereof |
| WO2023114901A2 (en) | 2021-12-15 | 2023-06-22 | Oxford Biomedica Solutions Llc | Methods and compositions for the production of adeno-associated virus |
| CN115896172A (zh) * | 2022-09-29 | 2023-04-04 | 深圳盛源生物技术有限公司 | 一种快速建立果蝇细胞稳定表达株的共转染载体及其制备方法和应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5888774A (en) | 1994-12-19 | 1999-03-30 | Cangene Corporation | Recombinant DNA molecules and expression vectors for erythropoietin |
| FR2738842B1 (fr) | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
| US5922583A (en) | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
| DE19848017A1 (de) | 1998-10-17 | 2000-04-20 | Multigene Biotech Gmbh | Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen |
| FR2821855B1 (fr) | 2001-03-09 | 2004-04-02 | Cayla | Genes synthetiques et plasmides bacteriens depourvus de cpg |
| GB0327056D0 (en) | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
| TWI311152B (en) | 2004-09-17 | 2009-06-21 | Boehringer Ingelheim Rcv Gmbh & Co K | Host-vector system for antibiotic-free cole1 plasmid propagation |
| CA2586201A1 (en) * | 2004-11-03 | 2006-05-11 | Almac Diagnostics Limited | Transcriptome microarray technology and methods of using the same |
| ATE504655T1 (de) * | 2005-10-05 | 2011-04-15 | Univ Milano Bicocca | Verfahren zur übertragung episomaler vektoren in tierzellen |
| US9109012B2 (en) | 2007-05-29 | 2015-08-18 | Nature Technology Corporation | Vectors and method for genetic immunization |
| WO2010006415A1 (en) | 2008-06-30 | 2010-01-21 | Atgcell Inc. | Mammalian cell expression vectors and utilization |
| EP3093351B1 (en) * | 2008-07-09 | 2018-04-18 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
| MX2011001644A (es) | 2008-08-12 | 2011-10-06 | Avesthagen Ltd | Un vector de expresion y un metodo del mismo. |
| US9045759B2 (en) | 2009-01-21 | 2015-06-02 | James Arthur Williams | DNA plasmids with improved copy number |
| EP2476755A1 (en) | 2011-01-17 | 2012-07-18 | Helmholtz-Zentrum für Infektionsforschung GmbH | S/MAR containing expression vectors, cell and cell lines as well as methods using the same |
| GB201213117D0 (en) | 2012-07-24 | 2012-09-05 | Ucl Business Plc | Transgene expression |
| CA2883227A1 (en) * | 2012-08-29 | 2014-03-06 | Nature Technology Corporation | Dna plasmids with improved expression |
| US20150322439A1 (en) * | 2012-11-19 | 2015-11-12 | Nature Technology Corporation | Replicative minicircle vectors with improved expression |
| EP2878674A1 (en) | 2013-11-28 | 2015-06-03 | Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) | Stable episomes based on non-integrative lentiviral vectors |
| JP6544565B2 (ja) | 2015-04-28 | 2019-07-17 | 国立大学法人広島大学 | 哺乳動物細胞内で目的遺伝子の発現を高める方法およびキット、並びに、その利用 |
| CA3132420A1 (en) * | 2019-03-18 | 2020-09-24 | Deutsches Krebsforschungszentrum | Expression constructs for the genetic modification of cells |
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2017
- 2017-09-19 ES ES17191829T patent/ES2821655T3/es active Active
- 2017-09-19 FI FIEP17191829.5T patent/FI3456821T4/fi active
- 2017-09-19 DK DK17191829.5T patent/DK3456821T4/da active
- 2017-09-19 EP EP17191829.5A patent/EP3456821B2/en active Active
-
2018
- 2018-09-17 WO PCT/US2018/051381 patent/WO2019060253A1/en active Application Filing
- 2018-09-17 US US16/648,885 patent/US20200277624A1/en active Pending
- 2018-09-18 CA CA3017658A patent/CA3017658A1/en active Pending
- 2018-09-19 US US16/649,064 patent/US20200263195A1/en not_active Abandoned
- 2018-09-19 CN CN201880061037.1A patent/CN111492056A/zh active Pending
- 2018-09-19 PT PT187693775T patent/PT3684924T/pt unknown
- 2018-09-19 EP EP18769378.3A patent/EP3684925B1/en active Active
- 2018-09-19 WO PCT/EP2018/075353 patent/WO2019057773A1/en unknown
- 2018-09-19 DK DK18769378.3T patent/DK3684925T3/da active
- 2018-09-19 EP EP22201555.4A patent/EP4186975A1/en active Pending
- 2018-09-19 ES ES18769378T patent/ES2887776T3/es active Active
- 2018-09-19 JP JP2020516915A patent/JP7189943B2/ja active Active
- 2018-09-19 CN CN201880061036.7A patent/CN111278972B/zh active Active
- 2018-09-19 JP JP2020516608A patent/JP7263327B2/ja active Active
- 2018-09-19 FI FIEP18769377.5T patent/FI3684924T3/fi active
- 2018-09-19 LT LTEPPCT/EP2018/075353T patent/LT3684924T/lt unknown
- 2018-09-19 PL PL18769377.5T patent/PL3684924T3/pl unknown
- 2018-09-19 HU HUE18769377A patent/HUE060893T2/hu unknown
- 2018-09-19 KR KR1020207010863A patent/KR20200054278A/ko not_active Application Discontinuation
- 2018-09-19 EP EP18769377.5A patent/EP3684924B1/en active Active
- 2018-09-19 US US16/648,768 patent/US20210403941A1/en active Pending
- 2018-09-19 SG SG11202001941SA patent/SG11202001941SA/en unknown
- 2018-09-19 KR KR1020207010722A patent/KR20200079244A/ko not_active Application Discontinuation
- 2018-09-19 AU AU2018337672A patent/AU2018337672A1/en active Pending
- 2018-09-19 AU AU2018337673A patent/AU2018337673A1/en active Pending
- 2018-09-19 CA CA3076285A patent/CA3076285A1/en active Pending
- 2018-09-19 DK DK18769377.5T patent/DK3684924T3/da active
- 2018-09-19 MX MX2020003092A patent/MX2020003092A/es unknown
- 2018-09-19 ES ES18769377T patent/ES2942210T3/es active Active
- 2018-09-19 SG SG11202002367WA patent/SG11202002367WA/en unknown
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2020
- 2020-03-12 IL IL273267A patent/IL273267A/en unknown
- 2020-03-17 IL IL273362A patent/IL273362A/en unknown
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2023
- 2023-04-12 JP JP2023064820A patent/JP2023089132A/ja active Pending
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