DK3981884T3 - Enkeltcelle-helgenombiblioteker til methyleringssekventering - Google Patents

Enkeltcelle-helgenombiblioteker til methyleringssekventering Download PDF

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Publication number
DK3981884T3
DK3981884T3 DK21203166.0T DK21203166T DK3981884T3 DK 3981884 T3 DK3981884 T3 DK 3981884T3 DK 21203166 T DK21203166 T DK 21203166T DK 3981884 T3 DK3981884 T3 DK 3981884T3
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Prior art keywords
single cell
whole genome
cell whole
genome libraries
methylation sequence
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DK21203166.0T
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English (en)
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Andrew C Adey
Ryan Mulqueen
Frank J Steemers
Steven Norberg
Dmitry K Pokholok
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Univ Oregon Health & Science
Illumina Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
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    • C12Q2523/125—Bisulfite(s)
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/159—Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

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  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK21203166.0T 2017-06-07 2018-06-05 Enkeltcelle-helgenombiblioteker til methyleringssekventering DK3981884T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762516324P 2017-06-07 2017-06-07
EP18734381.9A EP3635136B1 (en) 2017-06-07 2018-06-05 Single cell whole genome libraries for methylation sequencing

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DK3981884T3 true DK3981884T3 (da) 2023-09-04

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US (1) US20180355348A1 (da)
EP (3) EP3981884B1 (da)
JP (2) JP7407597B2 (da)
KR (2) KR102628035B1 (da)
CN (1) CN110997932B (da)
AU (1) AU2018280131A1 (da)
CA (1) CA3066424A1 (da)
DK (2) DK3635136T3 (da)
ES (2) ES2959047T3 (da)
FI (1) FI3981884T3 (da)
IL (2) IL271215A (da)
NZ (1) NZ759895A (da)
RU (1) RU2770879C2 (da)
SG (2) SG11201911730XA (da)
WO (1) WO2018226708A1 (da)

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SG11201911730XA (en) 2020-01-30
RU2770879C2 (ru) 2022-04-22
ES2959047T3 (es) 2024-02-19
AU2018280131A1 (en) 2020-01-02
KR102628035B1 (ko) 2024-01-22
KR20240013852A (ko) 2024-01-30
US20180355348A1 (en) 2018-12-13
EP3635136B1 (en) 2021-10-20
IL271241A (en) 2020-01-30
CA3066424A1 (en) 2018-12-13
DK3635136T3 (da) 2022-01-10
EP3981884B1 (en) 2023-08-09
EP3981884A1 (en) 2022-04-13
WO2018226708A1 (en) 2018-12-13
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RU2019144026A3 (da) 2021-10-07
RU2019144026A (ru) 2021-07-09
SG10202108175RA (en) 2021-09-29
EP3635136A1 (en) 2020-04-15
KR20200016287A (ko) 2020-02-14
CN110997932A (zh) 2020-04-10
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