MX2022001324A - Análisis de células individuales. - Google Patents

Análisis de células individuales.

Info

Publication number
MX2022001324A
MX2022001324A MX2022001324A MX2022001324A MX2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A MX 2022001324 A MX2022001324 A MX 2022001324A
Authority
MX
Mexico
Prior art keywords
methods
single cell
sequencing
analysis
cell
Prior art date
Application number
MX2022001324A
Other languages
English (en)
Inventor
Charles Gawad
Jay A A West
Original Assignee
Bioskryb Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioskryb Genomics Inc filed Critical Bioskryb Genomics Inc
Publication of MX2022001324A publication Critical patent/MX2022001324A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

En la presente se proporcionan composiciones y métodos para la amplificación precisa y escalable de ácidos nucleicos mediante amplificación dirigida por plantillas primaria (PTA) y métodos de secuenciación, y sus aplicaciones para el análisis mutacional en la investigación, diagnóstico y tratamiento. Además, en la presente se proporcionan métodos multiómicos para el análisis paralelo de ADN, ARN y/o proteínas de células individuales.
MX2022001324A 2019-07-31 2020-07-30 Análisis de células individuales. MX2022001324A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962881183P 2019-07-31 2019-07-31
PCT/US2020/044338 WO2021022085A2 (en) 2019-07-31 2020-07-30 Single cell analysis

Publications (1)

Publication Number Publication Date
MX2022001324A true MX2022001324A (es) 2022-05-19

Family

ID=74228691

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022001324A MX2022001324A (es) 2019-07-31 2020-07-30 Análisis de células individuales.

Country Status (10)

Country Link
US (1) US20230220377A1 (es)
EP (1) EP4004201A4 (es)
JP (1) JP2022543051A (es)
KR (1) KR20220041875A (es)
CN (1) CN114555802A (es)
AU (1) AU2020322027A1 (es)
CA (1) CA3149610A1 (es)
IL (1) IL290245A (es)
MX (1) MX2022001324A (es)
WO (1) WO2021022085A2 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113151425B (zh) * 2021-04-08 2023-01-06 中国计量科学研究院 基于关键指标提高准确性的单细胞测序方法
EP4330421A1 (en) * 2021-04-26 2024-03-06 The Brigham and Women's Hospital, Inc. Compositions and methods for characterizing polynucleotide sequence alterations
EP4388128A1 (en) * 2021-08-16 2024-06-26 Bioskryb Genomics, Inc. Embryonic nucleic acid analysis
WO2023055790A1 (en) * 2021-09-29 2023-04-06 Battelle Memorial Institute Apparatus and methods for performing multiple omics analysis and processing analyte mixtures
CN113943729B (zh) * 2021-10-20 2023-05-16 翌圣生物科技(上海)股份有限公司 U型接头及采用u型接头介导的磁珠偶联转座酶进行rna快速均一化建库的方法
WO2023212223A1 (en) * 2022-04-28 2023-11-02 BioSkryb Genomics, Inc. Single cell multiomics
WO2023215524A2 (en) * 2022-05-05 2023-11-09 BioSkryb Genomics, Inc. Primary template-directed amplification and methods thereof
CN115144519A (zh) * 2022-06-30 2022-10-04 上海交通大学 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用
WO2024026376A2 (en) * 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1924704T3 (da) * 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
US9309511B2 (en) * 2007-08-28 2016-04-12 The Johns Hopkins University Functional assay for identification of loss-of-function mutations in genes
WO2012071096A2 (en) * 2010-09-03 2012-05-31 The Johns Hopkins University Arid1a and ppp2r1a mutations in cancer
US10480021B2 (en) * 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
US11111519B2 (en) * 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
AU2017331459B2 (en) * 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
CN110462053A (zh) * 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序
CN111183145B (zh) * 2017-03-08 2024-01-09 芝加哥大学 高灵敏度dna甲基化分析方法
CN110997932B (zh) * 2017-06-07 2024-06-04 俄勒冈健康科学大学 用于甲基化测序的单细胞全基因组文库
SG11201911869XA (en) * 2017-08-01 2020-01-30 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS

Also Published As

Publication number Publication date
EP4004201A4 (en) 2023-08-23
WO2021022085A2 (en) 2021-02-04
CA3149610A1 (en) 2021-02-04
WO2021022085A3 (en) 2021-03-11
EP4004201A2 (en) 2022-06-01
JP2022543051A (ja) 2022-10-07
KR20220041875A (ko) 2022-04-01
AU2020322027A1 (en) 2022-03-03
US20230220377A1 (en) 2023-07-13
IL290245A (en) 2022-03-01
CN114555802A (zh) 2022-05-27

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