MX2020003092A - Vectores de adn no integrantes para la modificacion genetica de celulas. - Google Patents
Vectores de adn no integrantes para la modificacion genetica de celulas.Info
- Publication number
- MX2020003092A MX2020003092A MX2020003092A MX2020003092A MX2020003092A MX 2020003092 A MX2020003092 A MX 2020003092A MX 2020003092 A MX2020003092 A MX 2020003092A MX 2020003092 A MX2020003092 A MX 2020003092A MX 2020003092 A MX2020003092 A MX 2020003092A
- Authority
- MX
- Mexico
- Prior art keywords
- polynucleotide
- cells
- genetic modification
- dna vectors
- present
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2820/00—Vectors comprising a special origin of replication system
- C12N2820/55—Vectors comprising a special origin of replication system from bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
Abstract
La presente invención se refiere a un polinucleótido que comprende al menos un promotor y un elemento S/MAR, en donde dicho elemento S/MAR está situado corriente abajo de dicho promotor y en donde la secuencia de ácido nucleico de dicho elemento S/MAR (secuencia S/MAR) comprende al menos 3 motivos de secuencia ATTA (SEQ ID NO:1) por 100 nucleótidos en un tramo de como máximo 200 nucleótidos; la presente invención se refiere además a una composición y a una célula hospedera que comprende dicho polinucleótido, y al polinucleótido para usarse en medicina y para usarse en el tratamiento de enfermedades genéticas. La presente invención se refiere también a un kit y a un dispositivo que comprende dicho polinucleótido y a métodos y usos relacionados con el polinucleótido.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17191829.5A EP3456821B2 (en) | 2017-09-19 | 2017-09-19 | Non-integrating dna vectors for the genetic modification of cells |
| PCT/EP2018/075353 WO2019057773A1 (en) | 2017-09-19 | 2018-09-19 | NON-INTEGRATED DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020003092A true MX2020003092A (es) | 2020-07-28 |
Family
ID=60037350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020003092A MX2020003092A (es) | 2017-09-19 | 2018-09-19 | Vectores de adn no integrantes para la modificacion genetica de celulas. |
Country Status (18)
| Country | Link |
|---|---|
| US (3) | US20200277624A1 (es) |
| EP (4) | EP3456821B2 (es) |
| JP (3) | JP7189943B2 (es) |
| KR (2) | KR20200054278A (es) |
| CN (2) | CN111492056A (es) |
| AU (2) | AU2018337672A1 (es) |
| CA (2) | CA3017658A1 (es) |
| DK (3) | DK3456821T4 (es) |
| ES (3) | ES2821655T3 (es) |
| FI (2) | FI3456821T4 (es) |
| HU (1) | HUE060893T2 (es) |
| IL (2) | IL273267A (es) |
| LT (1) | LT3684924T (es) |
| MX (1) | MX2020003092A (es) |
| PL (1) | PL3684924T3 (es) |
| PT (1) | PT3684924T (es) |
| SG (2) | SG11202002367WA (es) |
| WO (2) | WO2019060253A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| CA3085784A1 (en) | 2017-12-14 | 2019-06-20 | Flodesign Sonics, Inc. | Acoustic transducer driver and controller |
| EP3942035A1 (en) * | 2019-03-18 | 2022-01-26 | Deutsches Krebsforschungszentrum | Expression constructs for the genetic modification of cells |
| CN115066255A (zh) | 2019-09-18 | 2022-09-16 | 星际治疗有限公司 | 合成的dna载体和使用方法 |
| WO2022018143A1 (en) | 2020-07-22 | 2022-01-27 | Deutsches Krebsforschungszentrum | 5' s/mar applications |
| CN113025651B (zh) * | 2021-03-31 | 2023-03-24 | 重庆医科大学 | 靶向HBV核心启动子的药物筛选细胞模型、Triciribine及结构类似物新应用 |
| AU2022299552A1 (en) | 2021-06-25 | 2024-01-04 | Oxford Biomedica (Us) Llc | Adeno-associated virus packaging systems |
| WO2023083985A1 (en) | 2021-11-11 | 2023-05-19 | Patrick Schmidt | Immunoreactive molecules and uses thereof |
| WO2023083982A1 (en) | 2021-11-11 | 2023-05-19 | Universitaet Heidelberg | Immunoreactive molecules and uses thereof |
| WO2023114901A2 (en) | 2021-12-15 | 2023-06-22 | Oxford Biomedica Solutions Llc | Methods and compositions for the production of adeno-associated virus |
| CN115896172A (zh) * | 2022-09-29 | 2023-04-04 | 深圳盛源生物技术有限公司 | 一种快速建立果蝇细胞稳定表达株的共转染载体及其制备方法和应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5888774A (en) | 1994-12-19 | 1999-03-30 | Cangene Corporation | Recombinant DNA molecules and expression vectors for erythropoietin |
| FR2738842B1 (fr) | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique |
| US5922583A (en) | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
| DE19848017A1 (de) | 1998-10-17 | 2000-04-20 | Multigene Biotech Gmbh | Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen |
| FR2821855B1 (fr) | 2001-03-09 | 2004-04-02 | Cayla | Genes synthetiques et plasmides bacteriens depourvus de cpg |
| GB0327056D0 (en) | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
| TWI311152B (en) | 2004-09-17 | 2009-06-21 | Boehringer Ingelheim Rcv Gmbh & Co K | Host-vector system for antibiotic-free cole1 plasmid propagation |
| CA2586201A1 (en) * | 2004-11-03 | 2006-05-11 | Almac Diagnostics Limited | Transcriptome microarray technology and methods of using the same |
| ATE504655T1 (de) * | 2005-10-05 | 2011-04-15 | Univ Milano Bicocca | Verfahren zur übertragung episomaler vektoren in tierzellen |
| EP2152889B1 (en) | 2007-05-29 | 2017-03-15 | Nature Technology Corporation | Vectors and methods for genetic immunization |
| CA2726862A1 (en) | 2008-06-30 | 2010-01-21 | Atgcell Inc. | Mammalian cell expression vectors and utilization |
| EP3093351B1 (en) * | 2008-07-09 | 2018-04-18 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
| KR20110044769A (ko) | 2008-08-12 | 2011-04-29 | 아베스타겐 리미티드 | 발현 벡터 및 그의 방법 |
| US9045759B2 (en) | 2009-01-21 | 2015-06-02 | James Arthur Williams | DNA plasmids with improved copy number |
| EP2476755A1 (en) | 2011-01-17 | 2012-07-18 | Helmholtz-Zentrum für Infektionsforschung GmbH | S/MAR containing expression vectors, cell and cell lines as well as methods using the same |
| GB201213117D0 (en) | 2012-07-24 | 2012-09-05 | Ucl Business Plc | Transgene expression |
| US9550998B2 (en) * | 2012-08-29 | 2017-01-24 | Nature Technology Corporation | DNA plasmids with improved expression |
| WO2014077863A1 (en) * | 2012-11-19 | 2014-05-22 | Nature Technology Corporation | Replicative minicircle vectors with improved expression |
| EP2878674A1 (en) | 2013-11-28 | 2015-06-03 | Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) | Stable episomes based on non-integrative lentiviral vectors |
| JP6544565B2 (ja) | 2015-04-28 | 2019-07-17 | 国立大学法人広島大学 | 哺乳動物細胞内で目的遺伝子の発現を高める方法およびキット、並びに、その利用 |
| EP3942035A1 (en) * | 2019-03-18 | 2022-01-26 | Deutsches Krebsforschungszentrum | Expression constructs for the genetic modification of cells |
-
2017
- 2017-09-19 ES ES17191829T patent/ES2821655T3/es active Active
- 2017-09-19 EP EP17191829.5A patent/EP3456821B2/en active Active
- 2017-09-19 DK DK17191829.5T patent/DK3456821T4/da active
- 2017-09-19 FI FIEP17191829.5T patent/FI3456821T4/fi active
-
2018
- 2018-09-17 WO PCT/US2018/051381 patent/WO2019060253A1/en active Application Filing
- 2018-09-17 US US16/648,885 patent/US20200277624A1/en active Pending
- 2018-09-18 CA CA3017658A patent/CA3017658A1/en active Pending
- 2018-09-19 SG SG11202002367WA patent/SG11202002367WA/en unknown
- 2018-09-19 HU HUE18769377A patent/HUE060893T2/hu unknown
- 2018-09-19 DK DK18769378.3T patent/DK3684925T3/da active
- 2018-09-19 EP EP18769378.3A patent/EP3684925B1/en active Active
- 2018-09-19 EP EP22201555.4A patent/EP4186975A1/en active Pending
- 2018-09-19 US US16/648,768 patent/US20210403941A1/en active Pending
- 2018-09-19 CN CN201880061037.1A patent/CN111492056A/zh active Pending
- 2018-09-19 MX MX2020003092A patent/MX2020003092A/es unknown
- 2018-09-19 LT LTEPPCT/EP2018/075353T patent/LT3684924T/lt unknown
- 2018-09-19 ES ES18769378T patent/ES2887776T3/es active Active
- 2018-09-19 US US16/649,064 patent/US20200263195A1/en not_active Abandoned
- 2018-09-19 EP EP18769377.5A patent/EP3684924B1/en active Active
- 2018-09-19 WO PCT/EP2018/075353 patent/WO2019057773A1/en unknown
- 2018-09-19 JP JP2020516915A patent/JP7189943B2/ja active Active
- 2018-09-19 KR KR1020207010863A patent/KR20200054278A/ko not_active Application Discontinuation
- 2018-09-19 DK DK18769377.5T patent/DK3684924T3/da active
- 2018-09-19 KR KR1020207010722A patent/KR20200079244A/ko not_active Application Discontinuation
- 2018-09-19 AU AU2018337672A patent/AU2018337672A1/en active Pending
- 2018-09-19 AU AU2018337673A patent/AU2018337673A1/en active Pending
- 2018-09-19 ES ES18769377T patent/ES2942210T3/es active Active
- 2018-09-19 PL PL18769377.5T patent/PL3684924T3/pl unknown
- 2018-09-19 JP JP2020516608A patent/JP7263327B2/ja active Active
- 2018-09-19 CA CA3076285A patent/CA3076285A1/en active Pending
- 2018-09-19 PT PT187693775T patent/PT3684924T/pt unknown
- 2018-09-19 SG SG11202001941SA patent/SG11202001941SA/en unknown
- 2018-09-19 FI FIEP18769377.5T patent/FI3684924T3/fi active
- 2018-09-19 CN CN201880061036.7A patent/CN111278972B/zh active Active
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2020
- 2020-03-12 IL IL273267A patent/IL273267A/en unknown
- 2020-03-17 IL IL273362A patent/IL273362A/en unknown
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2023
- 2023-04-12 JP JP2023064820A patent/JP2023089132A/ja active Pending
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