DK164407B - Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre - Google Patents

Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre Download PDF

Info

Publication number
DK164407B
DK164407B DK171382A DK171382A DK164407B DK 164407 B DK164407 B DK 164407B DK 171382 A DK171382 A DK 171382A DK 171382 A DK171382 A DK 171382A DK 164407 B DK164407 B DK 164407B
Authority
DK
Denmark
Prior art keywords
complex
double
purine
polynucleotide
compound
Prior art date
Application number
DK171382A
Other languages
Danish (da)
English (en)
Other versions
DK171382A (da
Inventor
David C Ward
Pennina R Langer-Safer
Iii Alexander A Waldrop
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22967380&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK164407(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Yale filed Critical Univ Yale
Publication of DK171382A publication Critical patent/DK171382A/da
Publication of DK164407B publication Critical patent/DK164407B/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18Testing for antimicrobial activity of a material
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK171382A 1981-04-17 1982-04-16 Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre DK164407B (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25522381A 1981-04-17 1981-04-17
US25522381 1981-04-17

Publications (2)

Publication Number Publication Date
DK171382A DK171382A (da) 1982-10-18
DK164407B true DK164407B (da) 1992-06-22

Family

ID=22967380

Family Applications (3)

Application Number Title Priority Date Filing Date
DK171382A DK164407B (da) 1981-04-17 1982-04-16 Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre
DK160591A DK171822B1 (da) 1981-04-17 1991-09-16 Modificerede nucleotider og fremgangsmåder til deres fremstilling og påvisning
DK046797A DK46797A (da) 1981-04-17 1997-04-28 Modificerede oligo- og polynucleotider og fremgangsmåder til deres fremstilling og påvisning

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK160591A DK171822B1 (da) 1981-04-17 1991-09-16 Modificerede nucleotider og fremgangsmåder til deres fremstilling og påvisning
DK046797A DK46797A (da) 1981-04-17 1997-04-28 Modificerede oligo- og polynucleotider og fremgangsmåder til deres fremstilling og påvisning

Country Status (8)

Country Link
EP (1) EP0063879B1 (ja)
JP (6) JPS57209297A (ja)
AT (1) ATE167189T1 (ja)
AU (1) AU560651B2 (ja)
CA (1) CA1219824A (ja)
DE (2) DE3280032D1 (ja)
DK (3) DK164407B (ja)
IL (1) IL65447A (ja)

Families Citing this family (186)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
DK105582A (da) * 1982-03-11 1983-09-12 Nordisk Insulinlab Fremgangsmaade til bestemmelse af humane hla-d(r) vaevstyper og gensonde til brug ved fremgangsmaaden
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US7220854B1 (en) 1982-06-23 2007-05-22 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Sugar moiety labeled nucleotide, and an oligo- or polynucleotide, and other compositions comprising such sugar moiety labeled nucleotides
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
DE3382053D1 (de) * 1982-09-30 1991-01-17 Iro Ab Garnspeicher- und zufuehrvorrichtung.
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
FR2540122B1 (fr) * 1983-01-27 1985-11-29 Centre Nat Rech Scient Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
IL70765A (en) * 1983-01-27 1988-07-31 Enzo Biochem Inc Substrates containing non-radioactive chemically-labeled polynucleotides and methods using them
US4605735A (en) * 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
JPS59148798A (ja) * 1983-02-14 1984-08-25 Wakunaga Seiyaku Kk ビオチンヌクレオチド誘導体
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
AU596068B2 (en) * 1983-02-22 1990-04-26 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
CA1254525A (en) * 1983-04-13 1989-05-23 Christine L. Brakel Kit for terminally chemically labeling dna
IL71699A0 (en) * 1983-05-02 1984-07-31 Enzo Biochem Inc Methods and kits for the analysis and detection of biological or genetic material
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US5221609A (en) * 1983-05-31 1993-06-22 Orgenics Ltd. Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
ATE78301T1 (de) * 1983-05-31 1992-08-15 Orgenics Ltd Molekulare genetische sonde und verfahren zu ihrer herstellung, testmethode und satz in denen diese molekulare genetische sonde gebraucht wird.
CA1222705A (en) * 1983-07-14 1987-06-09 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US4959309A (en) * 1983-07-14 1990-09-25 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US4623627A (en) 1983-08-19 1986-11-18 Cetus Corporation Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
WO1985002415A1 (fr) * 1983-11-24 1985-06-06 Institut Pasteur Sonde non radioactive et procede pour la caracterisation d'une sequence nucleotidique determinee et moyens pour la production de cette sonde non radioactive
FR2555606B1 (fr) * 1983-11-24 1986-10-24 Pasteur Institut Sonde non radio-active et procede pour la caracterisation d'une sequence nucleotidique determinee et moyens pour la production de cette sonde non radio-active
IL73578A (en) * 1983-12-12 1989-09-28 Miles Lab Method and reagent for the detection of polynucleotide sequence in sample of dna or rna by using a nucleic acid probe and contact of duplexes with immobilizing antibody
EP0144913A3 (en) * 1983-12-12 1986-08-20 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid
IL73580A0 (en) * 1983-12-12 1985-02-28 Miles Lab Hybridization assay employing labeled pairs of hybrid binding reagents
US4743535A (en) * 1984-11-07 1988-05-10 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid
EP0144914A3 (en) * 1983-12-12 1986-08-13 Miles Inc. Hybridization assay employing labeled pairs of hybrid binding reagents
EP0146039A3 (en) * 1983-12-12 1986-08-27 Miles Inc. Hybridization assay with immobilization of hybrids by antihybrid binding
AU583258B2 (en) * 1983-12-16 1989-04-27 Medisense Inc. Assay for nucleic acids
US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
JPH0690200B2 (ja) * 1984-01-16 1994-11-14 カリフオルニア・インステイテユ−ト・オブ・テクノロジ− オリゴヌクレオチドの分析法
AU582341B2 (en) * 1984-01-27 1989-03-23 Molecular Biosystems, Inc. Assay for immobilized reporter groups
US4699876A (en) * 1984-01-27 1987-10-13 E. I. Du Pont De Nemours And Company Nonradiometric polynucleotide probes
US4943523A (en) * 1984-01-30 1990-07-24 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5002885A (en) * 1984-01-30 1991-03-26 Enzo Biochem, Inc. Detectable molecules, method preparation and use
US4843122A (en) * 1984-01-30 1989-06-27 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4849505A (en) * 1984-01-30 1989-07-18 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4849208A (en) * 1984-01-30 1989-07-18 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
AU3844485A (en) * 1984-02-09 1985-08-15 Enzo Biochem Inc. Heterologous detection of cabeled dna
US4803297A (en) * 1984-03-21 1989-02-07 Cetus Corporation Carbamic acid ester useful for preparing a nucleic acid probe
US4751313A (en) * 1984-03-21 1988-06-14 Cetus Corporation Precursor to nucleic acid probe
US4617261A (en) * 1984-03-21 1986-10-14 Cetus Corporation Process for labeling nucleic acids and hybridization probes
US4705886A (en) * 1984-03-21 1987-11-10 Cetus Corporation Precursor to nucleic acid probe
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
NZ211888A (en) * 1984-05-10 1987-08-31 Abbott Lab Biotin-antibiotin immunoassay for detecting ligands
CA1253777A (en) * 1984-06-01 1989-05-09 Robert J. Carrico Nucleic acid hybridization assay employing immobilized rna probes
JPH0658368B2 (ja) * 1984-06-28 1994-08-03 株式会社日立製作所 核酸塩基検出装置
JP2648082B2 (ja) * 1984-06-28 1997-08-27 株式会社日立製作所 核酸塩基検出方法
FR2567523B1 (fr) * 1984-07-12 1987-11-13 Pasteur Institut Polynucleotides lies de facon covalente a un support solide, et procede pour leur obtention
DE3431536A1 (de) * 1984-08-28 1986-03-13 Boehringer Mannheim Gmbh, 6800 Mannheim Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes
US4828979A (en) * 1984-11-08 1989-05-09 Life Technologies, Inc. Nucleotide analogs for nucleic acid labeling and detection
US4754065A (en) * 1984-12-18 1988-06-28 Cetus Corporation Precursor to nucleic acid probe
US4670379A (en) * 1984-12-19 1987-06-02 E. I. Du Pont De Nemours And Company Polynucleotide hydridization assays employing catalyzed luminescence
US4824775A (en) * 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
EP0187332B1 (en) * 1985-01-10 1989-02-01 Molecular Diagnostics, Inc. Photochemical method of labelling nucleic acids for detection in hybridization assays
ATE43348T1 (de) * 1985-01-24 1989-06-15 Neopharmed Spa Acylierte cytidin-diphosphat-choline, verfahren zu ihrer herstellung und ihre therapeutische verwendung.
US4735897A (en) * 1985-05-02 1988-04-05 Allied Corporation Method and kit for detecting polyriboadenosine segments and messenger RNA
US4767699A (en) * 1985-05-02 1988-08-30 Allied Corporation Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection
US4762779A (en) * 1985-06-13 1988-08-09 Amgen Inc. Compositions and methods for functionalizing nucleic acids
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4833251A (en) * 1985-06-25 1989-05-23 Siska Diagnostics, Inc. Compounds for tagging nucleic acid probes
JPS622164A (ja) * 1985-06-25 1987-01-08 シスカ・ダイアグノステイツクス・インコ−ポレ−テツド N4−(置換アミノ)シチジンを含む核酸プロ−ブ
US4780405A (en) * 1985-06-25 1988-10-25 Siska Diagnostics, Inc. Carbonic anhydrase inhibitor-tagged nucleic acid probes
EP0235301B1 (en) * 1985-09-09 1992-07-22 Teijin Limited Pyridopyrimidine nucleotide derivatives
US5595908A (en) * 1985-09-26 1997-01-21 University Of Southern Mississipi Piezoelectric device for detection of polynucleotide hybridization
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4822731A (en) * 1986-01-09 1989-04-18 Cetus Corporation Process for labeling single-stranded nucleic acids and hybridizaiton probes
US5348855A (en) * 1986-03-05 1994-09-20 Miles Inc. Assay for nucleic acid sequences in an unpurified sample
US5091519A (en) * 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
GB8621337D0 (en) * 1986-09-04 1986-10-15 Agricultural Genetics Co Non-radioactive nucleic acid hybridization probes
JPH0799353B2 (ja) * 1987-03-31 1995-10-25 株式会社島津製作所 塩基配列決定装置
US5384241A (en) * 1987-09-11 1995-01-24 Enzo Diagnostics, Inc. Specific binding assay compound with inhibitive self-quenching characteristics
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
DE3800644A1 (de) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung
DE3813278A1 (de) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum nachweis von nukleinsaeuren
FR2627590A1 (fr) * 1988-02-24 1989-08-25 Ire Celltarg Sa Sonde d'acides nucleiques comportant un nucleotide terminal modifie chimiquement en 5(prime) (oh) en vue de son marquage non radioactif et procedes de preparation
FR2680374B1 (fr) * 1988-03-25 1993-11-12 Bioprobe Systems Sa Procede de coloration d'acides nucleiques detectes par marquage non radioactif et ensemble de reactifs pour la mise en óoeuvre de ce procede.
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5109124A (en) * 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
FR2632955B1 (fr) * 1988-06-20 1991-12-27 Oris Ind Derives de nucleosides utilisables pour la synthese d'oligonucleotides marques, oligonucleotides obtenus a partir de ces derives et leur synthese
US5489507A (en) * 1988-11-30 1996-02-06 Perkin-Elmer Corporation DNA detection by color complementation
DE3916595A1 (de) * 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
EP0407816A3 (en) * 1989-07-14 1993-01-27 Abbott Laboratories Base modified nucleosides
US6787338B2 (en) * 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5587472A (en) * 1991-08-13 1996-12-24 Bayer Corporation Coumarin-labeled nucleoside 5'-triphosphates
JP3390468B2 (ja) * 1991-10-16 2003-03-24 バイエル コーポレーション 新規の無水混合物法による遺伝子プローブの結合方法
WO1993011146A1 (en) * 1991-12-06 1993-06-10 University Of Miami Method and ring-opened purine compounds for labeling polynucleotides
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
DE4302459A1 (de) * 1993-01-29 1994-08-04 Bayer Ag Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) * 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5952172A (en) 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US5620850A (en) 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
DE4438918A1 (de) 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US5783387A (en) * 1995-02-06 1998-07-21 The Regents Of The University Of California Method for identifying and quantifying nucleic acid sequence aberrations
US5731153A (en) * 1996-08-26 1998-03-24 The Regents Of The University Of California Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions
EP0752474A1 (en) 1995-07-07 1997-01-08 Boehringer Mannheim Gmbh Nucleic acid coding for CMP-N-acetyl-neuraminic acid hydroxylase and its use for the production of modified glycoproteins
DE69637047T2 (de) * 1995-07-13 2007-12-27 Applera Corp., Foster City Unabhängiges gerät zur extraktion, amplifikation und nachweis von nukleinsäuren
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
US6444423B1 (en) 1996-06-07 2002-09-03 Molecular Dynamics, Inc. Nucleosides comprising polydentate ligands
DE19637042A1 (de) 1996-09-12 1998-03-19 Boehringer Mannheim Gmbh Heterocyclische Verbindungen und deren Verwendung in der Detektion von Nucleinsäuren
US7014992B1 (en) * 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7160678B1 (en) 1996-11-05 2007-01-09 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
AU764926B2 (en) 1998-01-27 2003-09-04 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
FR2780059B1 (fr) 1998-06-17 2002-10-11 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus
US6290839B1 (en) 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6761816B1 (en) 1998-06-23 2004-07-13 Clinical Micro Systems, Inc. Printed circuit boards with monolayers and capture ligands
US6335432B1 (en) * 1998-08-07 2002-01-01 Bio-Red Laboratories, Inc. Structural analogs of amine bases and nucleosides
US6740518B1 (en) 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
AU1241000A (en) 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
EP1006184A1 (en) 1998-12-03 2000-06-07 F. Hoffmann-La Roche Ag IGF-1 receptor interacting proteins (IIPs) genes coding therefor and uses thereof
US20020146728A1 (en) 1998-12-03 2002-10-10 Tanja Ligensa IGF-1 receptor interacting proteins
US6833267B1 (en) 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
ATE415409T1 (de) 1999-05-24 2008-12-15 Invitrogen Corp Ein verfahren zum entschützen markierter oligonukleotide
AU779689B2 (en) 1999-06-16 2005-02-03 Icos Corporation Human poly(ADP-ribose) polymerase 2 materials and methods
EP1218541B1 (en) 1999-07-26 2008-12-10 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6613516B1 (en) 1999-10-30 2003-09-02 Affymetrix, Inc. Preparation of nucleic acid samples
EP1099757B1 (en) 1999-11-11 2010-02-24 F. Hoffmann-La Roche Ag Process for the determination of CTp11 and for determining whether a tumor sample has metatastic potential
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
EP1111069A1 (en) * 1999-12-22 2001-06-27 BioChip Technologies GmbH Modified nucleic acids and their use
JP2001288197A (ja) 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd 蛍光ヌクレオチド
US6753143B2 (en) 2000-05-01 2004-06-22 Clinical Micro Sensors, Inc. Target analyte detection using asymmetrical self-assembled monolayers
EP1287129A1 (en) 2000-05-19 2003-03-05 F.Hoffmann-La Roche Ag A process for determing the tumoricidal potential of a sample by the use of a nucleic acid which is downregulated in human tumor cells
US7060441B2 (en) 2001-05-04 2006-06-13 Biomerieux Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
US7338805B2 (en) 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
US6893822B2 (en) 2001-07-19 2005-05-17 Nanogen Recognomics Gmbh Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
US7622544B2 (en) 2002-11-29 2009-11-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem Process for preparation of biodegradable polymers and resins from proteins, and plastics obtained thereby
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
FR2868071B1 (fr) 2004-03-26 2006-06-09 Biomerieux Sa Reactifs de marquage, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
JP4886679B2 (ja) * 2004-06-14 2012-02-29 マックス−プランク−ゲゼルシャフト ツール フォーデルング デル ヴィッセンシャフテン エー.ヴェー. 生体分子中のメチル化の配列特異的検出
US7928207B2 (en) * 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
DE602006014106D1 (de) 2005-03-14 2010-06-17 Link Genomics Inc Verfahren zur diagnose von prostatakrebs
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP1883709A4 (en) 2005-04-29 2010-10-13 Univ Rockefeller HUMAN MICRO-RNA AND METHODS OF INHIBITING THEREOF
FR2886735B1 (fr) 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
JP2007215496A (ja) * 2006-02-17 2007-08-30 Filgen Inc 核酸解析法におけるプローブ核酸の蛍光標識方法
CN101460619B (zh) 2006-06-06 2012-07-25 松下电器产业株式会社 核苷酸链的修饰方法
FR2917090B1 (fr) 2007-06-11 2012-06-15 Biomerieux Sa Reactifs de marquage portant des fonctions diazo et nitro, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2934595B1 (fr) 2008-07-29 2013-04-05 Biomerieux Sa Reactifs de marquage ayant un noyau pyridine portant une fonction diazomethyle, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
US20110262485A1 (en) 2008-08-04 2011-10-27 University Of Miami Sting (stimulator of interferon genes), a regulator of innate immune responses
WO2010101931A2 (en) 2009-03-03 2010-09-10 Access Medical System Co., Ltd. Detection system and method for high sensitivity fluorescent assays
US7928067B2 (en) 2009-05-14 2011-04-19 Ischemix Llc Compositions and methods for treating ischemia and ischemia-reperfusion injury
WO2011091435A2 (en) 2010-01-25 2011-07-28 Mount Sinai School Of Medicine Methods of treating liver disease
SG190794A1 (en) 2010-11-18 2013-07-31 Ischemix Llc Lipoyl compounds and their use for treating ischemic injury
FR2968302B1 (fr) 2010-12-06 2012-11-30 Biomerieux Sa Procedes de fonctionalisation et reactifs utilises dans de tels procedes utilisant un anhydride isatoique ou un de ses derives, molecules biologiques ainsi traitees et kits
CN105181659A (zh) 2011-04-20 2015-12-23 万迈医疗仪器有限公司 发光聚合物循环扩增
WO2013123305A1 (en) 2012-02-16 2013-08-22 The Penn State Research Foundation Modulators of acyl-coa lysocardiolipin acyltransferase 1 ( alcat1) and uses thereof
US9568431B2 (en) 2012-04-16 2017-02-14 Access Medical Systems, Ltd. Luminescent immunoassays for quantitating analytes having a wide concentration range
FR2994184B1 (fr) 2012-08-02 2017-12-08 Biomerieux Sa Procedes de fonctionalisation et reactifs utilises dans de tels procedes utilisant un anhydride aza-isatoique ou un de ses derives, molecules biologiques ainsi traitees et kits
CN107530399B (zh) 2014-10-10 2022-03-18 马萨诸塞眼科耳科诊所 治疗分子在体外和体内的有效递送
EP3212773B1 (en) 2014-10-29 2021-09-15 Massachusetts Eye and Ear Infirmary Efficient delivery of therapeutic molecules to cells of the inner ear
WO2016069912A1 (en) 2014-10-29 2016-05-06 Massachusetts Eye And Ear Infirmary Efficient delivery of therapeutic molecules in vitro and in vivo
US10744115B2 (en) 2017-04-25 2020-08-18 Ischemix Llc Compositions and methods for treating traumatic brain injury

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN142734B (ja) * 1975-04-28 1977-08-20 Miles Lab
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
FR2519004B1 (fr) * 1981-12-29 1985-09-27 Pasteur Institut Acide adenosine 5'-triphosphorique modifie et procede de dosage de substances biologiques susceptibles de fixer des produits de degradation de l'acide adenosine 5'-triphosphorique
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn

Also Published As

Publication number Publication date
DE3280478D1 (de) 1998-07-16
EP0063879A2 (en) 1982-11-03
DK46797A (da) 1997-04-28
AU560651B2 (en) 1987-04-16
JPH03261798A (ja) 1991-11-21
DK171382A (da) 1982-10-18
EP0063879A3 (en) 1983-08-10
JP3279503B2 (ja) 2002-04-30
ATE167189T1 (de) 1998-06-15
EP0063879B1 (en) 1989-11-23
JP3279502B2 (ja) 2002-04-30
DE3280478T2 (de) 1999-02-18
JPH07107998A (ja) 1995-04-25
DK160591D0 (da) 1991-09-16
JPS57209297A (en) 1982-12-22
DK160591A (da) 1991-09-16
CA1219824A (en) 1987-03-31
JPH1033199A (ja) 1998-02-10
IL65447A0 (en) 1982-07-30
JPS6399093A (ja) 1988-04-30
JPH0375559B2 (ja) 1991-12-02
IL65447A (en) 1987-01-30
JPH1052271A (ja) 1998-02-24
DE3280032D1 (en) 1989-12-28
DK171822B1 (da) 1997-06-23
AU8257382A (en) 1982-10-21
JPH0694474B2 (ja) 1994-11-24
JPH0880B2 (ja) 1996-01-10

Similar Documents

Publication Publication Date Title
DK164407B (da) Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre
US5328824A (en) Methods of using labeled nucleotides
EP0457824B1 (en) Detection of a nucleic acid sequence or a change therein
US6090592A (en) Method for performing amplification of nucleic acid on supports
US5856092A (en) Detection of a nucleic acid sequence or a change therein
US5641658A (en) Method for performing amplification of nucleic acid with two primers bound to a single solid support
EP0232967B1 (en) Competitive homogeneous assay
US5427911A (en) Coupled amplification and sequencing of DNA
US6048687A (en) Cycling DNA/RNA amplification electrochemiluminescent probe assay
AU599628B2 (en) Amplified hybridization assay
KR101590220B1 (ko) 유일 특이적 핵산 프로브 제조 방법
EP0329198B1 (en) Base modified oligo- and polynucleotides and methods of preparing and using same
CA2041355A1 (en) Restriction amplification assay
EP0405913A2 (en) Hydrophobic nucleic acid probe
US6468751B1 (en) Method and apparatus for performing amplification of nucleic acid on supports
JP2540482B2 (ja) ポリヌクレオチド配列の生体内標識化
WO1990008838A1 (en) Labeling of nucleic acids with fluorescent markers
JP3001919B2 (ja) 蛍光標識dnaの調製方法及びキット
Hilborne et al. Diagnostic applications of recombinant nucleic acid technology: basic techniques
JPH03123500A (ja) 核酸の分別方法
Ward et al. Trize Biochemical Analysis" 1992
Mardis Automated methods for DNA isolation and sequencing and their implementation

Legal Events

Date Code Title Description
PPF Opposition filed
PPF Opposition filed
B1 Patent granted (law 1993)
PPF Opposition filed
PDU Decision on opposition: patent maintained as unamended
PUP Patent expired