DK0620282T3 - Fremgangsmåde til ekstraktion, amplificering og påvisning af en nukleinsyre fra helblod - Google Patents

Fremgangsmåde til ekstraktion, amplificering og påvisning af en nukleinsyre fra helblod

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Publication number
DK0620282T3
DK0620282T3 DK94200344T DK94200344T DK0620282T3 DK 0620282 T3 DK0620282 T3 DK 0620282T3 DK 94200344 T DK94200344 T DK 94200344T DK 94200344 T DK94200344 T DK 94200344T DK 0620282 T3 DK0620282 T3 DK 0620282T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
detection
whole blood
amplification
extraction
Prior art date
Application number
DK94200344T
Other languages
English (en)
Inventor
Brent Arthur Burdick
Tobias Davis Ekeze
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Application granted granted Critical
Publication of DK0620282T3 publication Critical patent/DK0620282T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Perforating, Stamping-Out Or Severing By Means Other Than Cutting (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Vehicle Body Suspensions (AREA)
DK94200344T 1989-04-17 1990-04-09 Fremgangsmåde til ekstraktion, amplificering og påvisning af en nukleinsyre fra helblod DK0620282T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33943789A 1989-04-17 1989-04-17
US07/406,222 US5334499A (en) 1989-04-17 1989-09-12 Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction

Publications (1)

Publication Number Publication Date
DK0620282T3 true DK0620282T3 (da) 1998-09-14

Family

ID=26991623

Family Applications (1)

Application Number Title Priority Date Filing Date
DK94200344T DK0620282T3 (da) 1989-04-17 1990-04-09 Fremgangsmåde til ekstraktion, amplificering og påvisning af en nukleinsyre fra helblod

Country Status (9)

Country Link
US (1) US5334499A (da)
EP (2) EP0393744B1 (da)
JP (1) JPH072120B2 (da)
KR (1) KR900016463A (da)
AT (2) ATE162222T1 (da)
CA (1) CA2013316A1 (da)
DE (2) DE69016079T2 (da)
DK (1) DK0620282T3 (da)
FI (1) FI901917A0 (da)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
EP0504278B1 (en) * 1989-11-30 1997-01-15 Pharmacia Biotech AB A new method for detecting a specific nucleic acid sequence in a sample of cells
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
ATE143700T1 (de) * 1991-09-23 1996-10-15 Pfizer Verfahren für die detektion von spezifische mrns und dns in zellen
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
US5686238A (en) * 1992-02-10 1997-11-11 Baxter International Inc. Method and device for testing blood units for viral contamination
US5837453A (en) * 1992-05-13 1998-11-17 Geron Corporation Telomerase activity assays
US5629154A (en) * 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
DK0590327T3 (da) * 1992-09-11 2003-07-28 Hoffmann La Roche Påvisning af nukleinsyrer i blod
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB2282138B (en) * 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB9323305D0 (en) * 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
US5804380A (en) * 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
US5863726A (en) * 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
EP0904409A1 (de) * 1996-05-03 1999-03-31 Institut für Medizinische Molekulardiagnostik GbRmbH Verfahren zur dns-analytik von blut und mittel zur durchführung des verfahrens
US6322981B1 (en) * 1996-11-27 2001-11-27 The United States Of America As Represented By The Department Of Health And Human Services Rapid method for diagnosing the various forms of alpha-thalassemia
US5922288A (en) * 1997-05-29 1999-07-13 Herst; C. V. Taylor Device for isolating a component of a physiological sample
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
KR20010032806A (ko) * 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
EP2267127A1 (en) * 1998-04-28 2010-12-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
AU2862100A (en) * 1999-01-27 2000-08-18 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
WO2000050564A2 (en) * 1999-02-26 2000-08-31 Myriad Genetics, Inc. Method for extracting dna from dried specimens
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
DE60014399T2 (de) * 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
FR2822477B1 (fr) * 2001-03-23 2003-08-08 Inst Nat Sante Rech Med Methode de genotypage sans extraction de l'adn
US20040106109A1 (en) * 2001-10-02 2004-06-03 Belly Robert T Detection of ras mutations
WO2003062462A2 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US20040014097A1 (en) * 2002-05-06 2004-01-22 Mcglennen Ronald C. Genetic test apparatus and method
US8594948B2 (en) * 2002-09-18 2013-11-26 Ronald C. McGlennen Apparatus and methods for medical testing
US20060278242A1 (en) * 2005-03-23 2006-12-14 Mcglennen Ronald C Apparatus and methods for medical testing
US20060210988A1 (en) * 2003-04-22 2006-09-21 Ken Inose Method for isolating nucleic acid and, for nucleic acid isolation, kit and apparatus
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2006004611A2 (en) * 2004-06-25 2006-01-12 Invitrogen Corporation Separation of nucleic acid
US20080118926A1 (en) * 2004-12-13 2008-05-22 National University Corporation Okayama University Method For Detecting Methylation In Genes And Method For Examining Neoplasm Through Detecting Methylation In Genes
JP4765058B2 (ja) * 2004-12-13 2011-09-07 国立大学法人 岡山大学 核酸修飾前の検体の前処理方法
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US20060264714A1 (en) * 2005-03-23 2006-11-23 Mcglennen Ronald C Apparatus and methods for medical testing
WO2006122002A2 (en) * 2005-05-09 2006-11-16 Panomics, Inc. Multiplex capture of nucleic acids
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
CA2607221A1 (en) 2005-05-12 2006-11-23 Panomics, Inc. Multiplex branched-chain dna assays
US20080038725A1 (en) * 2005-06-20 2008-02-14 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
ES2276630B1 (es) * 2005-12-13 2008-06-16 Universitat Autonoma De Barcelona Metodo y kit para genotipificacion de hla-b basados en la pcr en tiempo real.
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US8039234B1 (en) * 2006-05-22 2011-10-18 Applied Biosystems, Llc Methods, panels of identification markers, and kits for identifying forensic samples
KR100785017B1 (ko) * 2006-06-05 2007-12-12 삼성전자주식회사 혈액으로부터 핵산을 증폭하는 방법
EP2035836B1 (en) * 2006-07-03 2013-02-27 Thermo Finnigan LLC Device for processing whole blood for analyte determination
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
JP2008167722A (ja) 2007-01-15 2008-07-24 Konica Minolta Medical & Graphic Inc 磁性支持体上での加熱による核酸単離方法
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
AU2008276211B2 (en) 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8895240B2 (en) * 2008-05-19 2014-11-25 E I Du Pont De Nemours And Company Method, kit and system for collecting and processing samples for DNA and RNA detection
CN103119178B (zh) 2010-09-22 2017-05-17 株式会社钟化 核酸的检测方法以及装置、试剂盒
ES2648564T3 (es) 2010-10-21 2018-01-04 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
JP5624487B2 (ja) * 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
US20180135108A1 (en) * 2014-01-20 2018-05-17 Board Of Trustees Of Michigan State University Method for detecting bacterial and fungal pathogens
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
ES2893100T3 (es) 2015-10-12 2022-02-08 Advanced Cell Diagnostics Inc Detección in situ de variantes de nucleótidos en muestras con un elevado nivel de ruido, y composiciones y métodos relacionados con ésta
BR102018003245A2 (pt) 2018-02-20 2019-09-10 Fundação Oswaldo Cruz oligonucleotídeo, conjunto de oligonucleotídeos, método para detecção simultânea de neisseria meningitidis, streptococcus pneumoniae e haemophilus influenzae, e, kit.
WO2021061797A1 (en) * 2019-09-24 2021-04-01 Genepath Diagnostics Inc. Composition and method for improving detection of biomolecules in biofluid samples
CN112458051A (zh) * 2020-11-11 2021-03-09 海南优尼科尔生物科技有限公司 一种外周血单个核细胞的提取收集方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1202743B (de) * 1960-12-28 1965-10-14 Toyo Boseki Verfahren zur Extraktion von Ribonucleinsaeure aus Hefe
US4515890A (en) * 1982-11-08 1985-05-07 Abbott Laboratories Immunoassay of terminal deoxynucleotidyl transferase
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
US4750982A (en) * 1985-03-21 1988-06-14 Lifecodes Corp. Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
EP0240191A3 (en) * 1986-03-13 1989-10-25 Seiko Instruments Inc. Method of isolating dna contained in a virus or cell
EP0245945B1 (en) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automated nucleic acid extractor

Also Published As

Publication number Publication date
ATE162222T1 (de) 1998-01-15
JPH072120B2 (ja) 1995-01-18
JPH02292298A (ja) 1990-12-03
EP0620282A1 (en) 1994-10-19
DE69016079T2 (de) 1995-09-14
EP0393744B1 (en) 1995-01-18
US5334499A (en) 1994-08-02
DE69031957T2 (de) 1998-06-10
KR900016463A (ko) 1990-11-13
DE69016079D1 (de) 1995-03-02
EP0393744A1 (en) 1990-10-24
ATE117377T1 (de) 1995-02-15
CA2013316A1 (en) 1990-10-17
FI901917A0 (fi) 1990-04-17
DE69031957D1 (de) 1998-02-19
EP0620282B1 (en) 1998-01-14

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