DE69016079D1 - Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. - Google Patents

Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut.

Info

Publication number
DE69016079D1
DE69016079D1 DE69016079T DE69016079T DE69016079D1 DE 69016079 D1 DE69016079 D1 DE 69016079D1 DE 69016079 T DE69016079 T DE 69016079T DE 69016079 T DE69016079 T DE 69016079T DE 69016079 D1 DE69016079 D1 DE 69016079D1
Authority
DE
Germany
Prior art keywords
nucleic acid
extracting
amplifying
fraction
detecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69016079T
Other languages
English (en)
Other versions
DE69016079T2 (de
Inventor
Brent A Burdick
Tobias D Ekeze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Application granted granted Critical
Publication of DE69016079D1 publication Critical patent/DE69016079D1/de
Publication of DE69016079T2 publication Critical patent/DE69016079T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Perforating, Stamping-Out Or Severing By Means Other Than Cutting (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Vehicle Body Suspensions (AREA)
DE69016079T 1989-04-17 1990-04-09 Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. Expired - Lifetime DE69016079T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33943789A 1989-04-17 1989-04-17
US07/406,222 US5334499A (en) 1989-04-17 1989-09-12 Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction

Publications (2)

Publication Number Publication Date
DE69016079D1 true DE69016079D1 (de) 1995-03-02
DE69016079T2 DE69016079T2 (de) 1995-09-14

Family

ID=26991623

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69016079T Expired - Lifetime DE69016079T2 (de) 1989-04-17 1990-04-09 Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut.
DE69031957T Expired - Lifetime DE69031957T2 (de) 1989-04-17 1990-04-09 Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69031957T Expired - Lifetime DE69031957T2 (de) 1989-04-17 1990-04-09 Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut

Country Status (9)

Country Link
US (1) US5334499A (de)
EP (2) EP0393744B1 (de)
JP (1) JPH072120B2 (de)
KR (1) KR900016463A (de)
AT (2) ATE162222T1 (de)
CA (1) CA2013316A1 (de)
DE (2) DE69016079T2 (de)
DK (1) DK0620282T3 (de)
FI (1) FI901917A0 (de)

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CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
ATE143700T1 (de) * 1991-09-23 1996-10-15 Pfizer Verfahren für die detektion von spezifische mrns und dns in zellen
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
US5686238A (en) * 1992-02-10 1997-11-11 Baxter International Inc. Method and device for testing blood units for viral contamination
US5837453A (en) * 1992-05-13 1998-11-17 Geron Corporation Telomerase activity assays
US5629154A (en) * 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
DK0590327T3 (da) * 1992-09-11 2003-07-28 Hoffmann La Roche Påvisning af nukleinsyrer i blod
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
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US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
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US6322981B1 (en) * 1996-11-27 2001-11-27 The United States Of America As Represented By The Department Of Health And Human Services Rapid method for diagnosing the various forms of alpha-thalassemia
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Also Published As

Publication number Publication date
ATE162222T1 (de) 1998-01-15
JPH072120B2 (ja) 1995-01-18
JPH02292298A (ja) 1990-12-03
EP0620282A1 (de) 1994-10-19
DE69016079T2 (de) 1995-09-14
EP0393744B1 (de) 1995-01-18
US5334499A (en) 1994-08-02
DE69031957T2 (de) 1998-06-10
KR900016463A (ko) 1990-11-13
DK0620282T3 (da) 1998-09-14
EP0393744A1 (de) 1990-10-24
ATE117377T1 (de) 1995-02-15
CA2013316A1 (en) 1990-10-17
FI901917A0 (fi) 1990-04-17
DE69031957D1 (de) 1998-02-19
EP0620282B1 (de) 1998-01-14

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