DE69016079T2 - Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. - Google Patents
Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut.Info
- Publication number
- DE69016079T2 DE69016079T2 DE69016079T DE69016079T DE69016079T2 DE 69016079 T2 DE69016079 T2 DE 69016079T2 DE 69016079 T DE69016079 T DE 69016079T DE 69016079 T DE69016079 T DE 69016079T DE 69016079 T2 DE69016079 T2 DE 69016079T2
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- extracting
- amplifying
- fraction
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70539—MHC-molecules, e.g. HLA-molecules
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Perforating, Stamping-Out Or Severing By Means Other Than Cutting (AREA)
- Manufacture Of Macromolecular Shaped Articles (AREA)
- Vehicle Body Suspensions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33943789A | 1989-04-17 | 1989-04-17 | |
US07/406,222 US5334499A (en) | 1989-04-17 | 1989-09-12 | Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69016079D1 DE69016079D1 (de) | 1995-03-02 |
DE69016079T2 true DE69016079T2 (de) | 1995-09-14 |
Family
ID=26991623
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69016079T Expired - Lifetime DE69016079T2 (de) | 1989-04-17 | 1990-04-09 | Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. |
DE69031957T Expired - Lifetime DE69031957T2 (de) | 1989-04-17 | 1990-04-09 | Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69031957T Expired - Lifetime DE69031957T2 (de) | 1989-04-17 | 1990-04-09 | Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut |
Country Status (9)
Country | Link |
---|---|
US (1) | US5334499A (de) |
EP (2) | EP0393744B1 (de) |
JP (1) | JPH072120B2 (de) |
KR (1) | KR900016463A (de) |
AT (2) | ATE162222T1 (de) |
CA (1) | CA2013316A1 (de) |
DE (2) | DE69016079T2 (de) |
DK (1) | DK0620282T3 (de) |
FI (1) | FI901917A0 (de) |
Families Citing this family (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
EP0504278B1 (de) * | 1989-11-30 | 1997-01-15 | Pharmacia Biotech AB | Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe |
CA2065719A1 (en) | 1991-04-30 | 1992-10-31 | John B. Findlay | Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle |
ATE143700T1 (de) * | 1991-09-23 | 1996-10-15 | Pfizer | Verfahren für die detektion von spezifische mrns und dns in zellen |
AU664050B2 (en) * | 1991-12-18 | 1995-11-02 | Becton Dickinson & Company | Process for lysing mycobacteria |
US5686238A (en) * | 1992-02-10 | 1997-11-11 | Baxter International Inc. | Method and device for testing blood units for viral contamination |
US5837453A (en) * | 1992-05-13 | 1998-11-17 | Geron Corporation | Telomerase activity assays |
US5629154A (en) * | 1993-11-12 | 1997-05-13 | Geron Corporation | Telomerase activity assays |
DK0590327T3 (da) * | 1992-09-11 | 2003-07-28 | Hoffmann La Roche | Påvisning af nukleinsyrer i blod |
IL110463A0 (en) * | 1993-08-13 | 1994-10-21 | Du Pont | In situ extraction of microbial DNA |
JP3451667B2 (ja) * | 1993-08-24 | 2003-09-29 | 東ソー株式会社 | 核酸抽出及び特定核酸配列の検出方法 |
US6043032A (en) * | 1993-09-22 | 2000-03-28 | Tosoh Corporation | Method of extracting nucleic acids and method of detecting specified nucleic acid sequences |
GB2282138B (en) * | 1993-09-28 | 1997-09-03 | Tosoh Corp | Method of extracting nucleic acids and method of detecting specified nucleic acid sequences |
GB9323305D0 (en) * | 1993-11-11 | 1994-01-05 | Medinnova Sf | Isoaltion of nucleic acid |
US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
US5863726A (en) * | 1993-11-12 | 1999-01-26 | Geron Corporation | Telomerase activity assays |
US5622822A (en) | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
US5705366A (en) | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
DE19530132C2 (de) * | 1995-08-16 | 1998-07-16 | Max Planck Gesellschaft | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
EP0904409A1 (de) * | 1996-05-03 | 1999-03-31 | Institut für Medizinische Molekulardiagnostik GbRmbH | Verfahren zur dns-analytik von blut und mittel zur durchführung des verfahrens |
US6322981B1 (en) * | 1996-11-27 | 2001-11-27 | The United States Of America As Represented By The Department Of Health And Human Services | Rapid method for diagnosing the various forms of alpha-thalassemia |
US5922288A (en) * | 1997-05-29 | 1999-07-13 | Herst; C. V. Taylor | Device for isolating a component of a physiological sample |
US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
KR20010032806A (ko) * | 1997-12-06 | 2001-04-25 | 매튜 존 베이커 | 핵산 분리방법 |
EP2267127A1 (de) * | 1998-04-28 | 2010-12-29 | Ortho-Clinical Diagnostics, Inc. | Verbesserte Verfahren zur Gewinnung von Nukleinsäuren aus Gewebeproben und aus paraffin-eingebetteten Gewebe |
AU2862100A (en) * | 1999-01-27 | 2000-08-18 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
WO2000050564A2 (en) * | 1999-02-26 | 2000-08-31 | Myriad Genetics, Inc. | Method for extracting dna from dried specimens |
US6469159B1 (en) | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
DE60014399T2 (de) * | 1999-05-04 | 2005-12-08 | Ortho-Clinical Diagnostics, Inc. | Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien |
US7262006B1 (en) | 2000-05-01 | 2007-08-28 | Ortho-Clinical Diagnostics, Inc. | Rapid and efficient capture of DNA from sample without using cell lysing reagent |
FR2822477B1 (fr) * | 2001-03-23 | 2003-08-08 | Inst Nat Sante Rech Med | Methode de genotypage sans extraction de l'adn |
US20040106109A1 (en) * | 2001-10-02 | 2004-06-03 | Belly Robert T | Detection of ras mutations |
WO2003062462A2 (en) | 2002-01-16 | 2003-07-31 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
US20040014097A1 (en) * | 2002-05-06 | 2004-01-22 | Mcglennen Ronald C. | Genetic test apparatus and method |
US8594948B2 (en) * | 2002-09-18 | 2013-11-26 | Ronald C. McGlennen | Apparatus and methods for medical testing |
US20060278242A1 (en) * | 2005-03-23 | 2006-12-14 | Mcglennen Ronald C | Apparatus and methods for medical testing |
US20060210988A1 (en) * | 2003-04-22 | 2006-09-21 | Ken Inose | Method for isolating nucleic acid and, for nucleic acid isolation, kit and apparatus |
EP2402089A1 (de) | 2003-07-31 | 2012-01-04 | Handylab, Inc. | Verarbeitung partikelhaltiger Proben |
US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
WO2006004611A2 (en) * | 2004-06-25 | 2006-01-12 | Invitrogen Corporation | Separation of nucleic acid |
US20080118926A1 (en) * | 2004-12-13 | 2008-05-22 | National University Corporation Okayama University | Method For Detecting Methylation In Genes And Method For Examining Neoplasm Through Detecting Methylation In Genes |
JP4765058B2 (ja) * | 2004-12-13 | 2011-09-07 | 国立大学法人 岡山大学 | 核酸修飾前の検体の前処理方法 |
US9505846B2 (en) | 2005-01-28 | 2016-11-29 | Life Technologies Corporation | Multi-component inhibitors of nucleic acid polymerases |
US20060264714A1 (en) * | 2005-03-23 | 2006-11-23 | Mcglennen Ronald C | Apparatus and methods for medical testing |
WO2006122002A2 (en) * | 2005-05-09 | 2006-11-16 | Panomics, Inc. | Multiplex capture of nucleic acids |
US8632970B2 (en) | 2005-05-09 | 2014-01-21 | Affymetrix, Inc. | Multiplex capture of nucleic acids |
CA2607221A1 (en) | 2005-05-12 | 2006-11-23 | Panomics, Inc. | Multiplex branched-chain dna assays |
US20080038725A1 (en) * | 2005-06-20 | 2008-02-14 | Yuling Luo | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
WO2007044427A2 (en) * | 2005-10-05 | 2007-04-19 | Panomics, Inc. | Detection of nucleic acids from whole blood |
ES2276630B1 (es) * | 2005-12-13 | 2008-06-16 | Universitat Autonoma De Barcelona | Metodo y kit para genotipificacion de hla-b basados en la pcr en tiempo real. |
US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
EP2001990B1 (de) | 2006-03-24 | 2016-06-29 | Handylab, Inc. | Integriertes system zur verarbeitung von mikrofluidischen proben und verwendungsverfahren |
US8039234B1 (en) * | 2006-05-22 | 2011-10-18 | Applied Biosystems, Llc | Methods, panels of identification markers, and kits for identifying forensic samples |
KR100785017B1 (ko) * | 2006-06-05 | 2007-12-12 | 삼성전자주식회사 | 혈액으로부터 핵산을 증폭하는 방법 |
EP2035836B1 (de) * | 2006-07-03 | 2013-02-27 | Thermo Finnigan LLC | Vorrichtung zur behandlung von vollblut für die bestimmung von analyten |
WO2008060604A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
JP2008167722A (ja) | 2007-01-15 | 2008-07-24 | Konica Minolta Medical & Graphic Inc | 磁性支持体上での加熱による核酸単離方法 |
US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
AU2008276211B2 (en) | 2007-07-13 | 2015-01-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
US8895240B2 (en) * | 2008-05-19 | 2014-11-25 | E I Du Pont De Nemours And Company | Method, kit and system for collecting and processing samples for DNA and RNA detection |
CN103119178B (zh) | 2010-09-22 | 2017-05-17 | 株式会社钟化 | 核酸的检测方法以及装置、试剂盒 |
ES2648564T3 (es) | 2010-10-21 | 2018-01-04 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
JP5624487B2 (ja) * | 2011-01-31 | 2014-11-12 | 横河電機株式会社 | 核酸抽出方法 |
ES2769028T3 (es) | 2011-04-15 | 2020-06-24 | Becton Dickinson Co | Termociclador microfluídico de barrido en tiempo real |
WO2013067202A1 (en) | 2011-11-04 | 2013-05-10 | Handylab, Inc. | Polynucleotide sample preparation device |
US20180135108A1 (en) * | 2014-01-20 | 2018-05-17 | Board Of Trustees Of Michigan State University | Method for detecting bacterial and fungal pathogens |
US10260111B1 (en) | 2014-01-20 | 2019-04-16 | Brett Eric Etchebarne | Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample |
ES2893100T3 (es) | 2015-10-12 | 2022-02-08 | Advanced Cell Diagnostics Inc | Detección in situ de variantes de nucleótidos en muestras con un elevado nivel de ruido, y composiciones y métodos relacionados con ésta |
BR102018003245A2 (pt) | 2018-02-20 | 2019-09-10 | Fundação Oswaldo Cruz | oligonucleotídeo, conjunto de oligonucleotídeos, método para detecção simultânea de neisseria meningitidis, streptococcus pneumoniae e haemophilus influenzae, e, kit. |
WO2021061797A1 (en) * | 2019-09-24 | 2021-04-01 | Genepath Diagnostics Inc. | Composition and method for improving detection of biomolecules in biofluid samples |
CN112458051A (zh) * | 2020-11-11 | 2021-03-09 | 海南优尼科尔生物科技有限公司 | 一种外周血单个核细胞的提取收集方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1202743B (de) * | 1960-12-28 | 1965-10-14 | Toyo Boseki | Verfahren zur Extraktion von Ribonucleinsaeure aus Hefe |
US4515890A (en) * | 1982-11-08 | 1985-05-07 | Abbott Laboratories | Immunoassay of terminal deoxynucleotidyl transferase |
GB8331071D0 (en) * | 1983-11-22 | 1983-12-29 | Karayiannis P | Assay for dna/rna |
US4750982A (en) * | 1985-03-21 | 1988-06-14 | Lifecodes Corp. | Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
CA1284931C (en) * | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
EP0240191A3 (de) * | 1986-03-13 | 1989-10-25 | Seiko Instruments Inc. | Verfahren zur Isolierung von in einem Virus oder einer Zelle enthaltender DNS |
EP0245945B1 (de) * | 1986-04-11 | 1993-11-10 | Applied Biosystems, Inc. | Automatisches Gerät zum Extrahieren von Nukleinsäuren |
-
1989
- 1989-09-12 US US07/406,222 patent/US5334499A/en not_active Expired - Lifetime
-
1990
- 1990-03-28 CA CA002013316A patent/CA2013316A1/en not_active Abandoned
- 1990-04-09 EP EP90200844A patent/EP0393744B1/de not_active Expired - Lifetime
- 1990-04-09 AT AT94200344T patent/ATE162222T1/de not_active IP Right Cessation
- 1990-04-09 DE DE69016079T patent/DE69016079T2/de not_active Expired - Lifetime
- 1990-04-09 EP EP94200344A patent/EP0620282B1/de not_active Expired - Lifetime
- 1990-04-09 AT AT90200844T patent/ATE117377T1/de not_active IP Right Cessation
- 1990-04-09 DE DE69031957T patent/DE69031957T2/de not_active Expired - Lifetime
- 1990-04-09 DK DK94200344T patent/DK0620282T3/da active
- 1990-04-16 JP JP2097789A patent/JPH072120B2/ja not_active Expired - Lifetime
- 1990-04-17 FI FI901917A patent/FI901917A0/fi not_active IP Right Cessation
- 1990-04-17 KR KR1019900005347A patent/KR900016463A/ko not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
ATE162222T1 (de) | 1998-01-15 |
JPH072120B2 (ja) | 1995-01-18 |
JPH02292298A (ja) | 1990-12-03 |
EP0620282A1 (de) | 1994-10-19 |
EP0393744B1 (de) | 1995-01-18 |
US5334499A (en) | 1994-08-02 |
DE69031957T2 (de) | 1998-06-10 |
KR900016463A (ko) | 1990-11-13 |
DK0620282T3 (da) | 1998-09-14 |
DE69016079D1 (de) | 1995-03-02 |
EP0393744A1 (de) | 1990-10-24 |
ATE117377T1 (de) | 1995-02-15 |
CA2013316A1 (en) | 1990-10-17 |
FI901917A0 (fi) | 1990-04-17 |
DE69031957D1 (de) | 1998-02-19 |
EP0620282B1 (de) | 1998-01-14 |
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