DE69019770D1 - Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure. - Google Patents

Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure.

Info

Publication number
DE69019770D1
DE69019770D1 DE69019770T DE69019770T DE69019770D1 DE 69019770 D1 DE69019770 D1 DE 69019770D1 DE 69019770 T DE69019770 T DE 69019770T DE 69019770 T DE69019770 T DE 69019770T DE 69019770 D1 DE69019770 D1 DE 69019770D1
Authority
DE
Germany
Prior art keywords
nucleic acid
detection
purification
multiplication
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69019770T
Other languages
English (en)
Other versions
DE69019770T2 (de
Inventor
Fred Terry Oakes
Brent Arthur Burdick
Janet Linn Fyles
Chu-An Chang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Eastman Kodak Co
Original Assignee
Cetus Corp
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp, Eastman Kodak Co filed Critical Cetus Corp
Application granted granted Critical
Publication of DE69019770D1 publication Critical patent/DE69019770D1/de
Publication of DE69019770T2 publication Critical patent/DE69019770T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DE69019770T 1989-03-17 1990-03-14 Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure. Expired - Lifetime DE69019770T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32531189A 1989-03-17 1989-03-17
US47506890A 1990-02-05 1990-02-05

Publications (2)

Publication Number Publication Date
DE69019770D1 true DE69019770D1 (de) 1995-07-06
DE69019770T2 DE69019770T2 (de) 1995-11-30

Family

ID=26984879

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69019770T Expired - Lifetime DE69019770T2 (de) 1989-03-17 1990-03-14 Verfahren zur Reinigung, Vervielfachung und zum Nachweis einer Nukleinsäure.

Country Status (7)

Country Link
EP (1) EP0388171B1 (de)
JP (1) JP2609738B2 (de)
KR (1) KR920007664B1 (de)
AT (1) ATE123315T1 (de)
CA (1) CA2011818A1 (de)
DE (1) DE69019770T2 (de)
HK (1) HK58397A (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
CA2032203C (en) * 1989-12-29 2009-05-19 Christine L. Brakel Amplification capture assay
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
US5589333A (en) * 1992-02-03 1996-12-31 Thomas Jefferson University In situ polymerase chain reaction
US7166423B1 (en) 1992-10-21 2007-01-23 Miltenyi Biotec Gmbh Direct selection of cells by secretion product
EP0763135B1 (de) * 1994-05-28 2002-07-10 Tepnel Medical Limited Herstellung von nukleinsaeurekopien

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
EP0200113A3 (de) * 1985-04-30 1987-03-18 Pandex Laboratories, Inc. Verfahren der Nukleinsäuren-Hybridisierungsprobe unter Zusatz eines lumineszierenden Labels
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
EP0365595A4 (de) * 1987-06-26 1990-06-05 Du Pont Abtrennung kontaminierter sequenzen von rekombinanter geklonter dns unter verwendung von affinitätspartikeln.
CA1297432C (en) * 1987-07-29 1992-03-17 Gulilat Gebeyehu Nucleic acid capture reagent
FI80476C (fi) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.

Also Published As

Publication number Publication date
EP0388171B1 (de) 1995-05-31
DE69019770T2 (de) 1995-11-30
ATE123315T1 (de) 1995-06-15
EP0388171A2 (de) 1990-09-19
KR900014593A (ko) 1990-10-24
CA2011818A1 (en) 1990-09-17
JP2609738B2 (ja) 1997-05-14
KR920007664B1 (ko) 1992-09-14
EP0388171A3 (de) 1991-06-05
HK58397A (en) 1997-05-09
JPH0341087A (ja) 1991-02-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: BOEHMERT & BOEHMERT, 28209 BREMEN