DE69133582D1 - Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase - Google Patents

Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase

Info

Publication number
DE69133582D1
DE69133582D1 DE69133582T DE69133582T DE69133582D1 DE 69133582 D1 DE69133582 D1 DE 69133582D1 DE 69133582 T DE69133582 T DE 69133582T DE 69133582 T DE69133582 T DE 69133582T DE 69133582 D1 DE69133582 D1 DE 69133582D1
Authority
DE
Germany
Prior art keywords
ligase
detection
nucleic acid
thermostabile
dna amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69133582T
Other languages
English (en)
Other versions
DE69133582T2 (de
Inventor
Francis Barany
John Zebala
Deborah A Nickerson
Robert J Kaiser
Leroy Hood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Cornell Research Foundation Inc
Original Assignee
California Institute of Technology CalTech
Cornell Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology CalTech, Cornell Research Foundation Inc filed Critical California Institute of Technology CalTech
Application granted granted Critical
Publication of DE69133582D1 publication Critical patent/DE69133582D1/de
Publication of DE69133582T2 publication Critical patent/DE69133582T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
DE69133582T 1990-05-03 1991-04-29 Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase Expired - Lifetime DE69133582T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51844790A 1990-05-03 1990-05-03
US518447 1990-05-03
PCT/US1991/002968 WO1991017239A1 (en) 1990-05-03 1991-04-29 A thermostable ligase mediated dna amplification system for the detection of genetic diseases

Publications (2)

Publication Number Publication Date
DE69133582D1 true DE69133582D1 (de) 2007-11-22
DE69133582T2 DE69133582T2 (de) 2008-07-24

Family

ID=24063976

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69133629T Expired - Lifetime DE69133629D1 (de) 1990-05-03 1991-04-29 nssystem zur Bestimmung von genetischen Krankheiten
DE69133582T Expired - Lifetime DE69133582T2 (de) 1990-05-03 1991-04-29 Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69133629T Expired - Lifetime DE69133629D1 (de) 1990-05-03 1991-04-29 nssystem zur Bestimmung von genetischen Krankheiten

Country Status (7)

Country Link
EP (2) EP0528882B1 (de)
JP (3) JP3889807B2 (de)
AT (2) ATE459709T1 (de)
DE (2) DE69133629D1 (de)
DK (1) DK0528882T3 (de)
ES (1) ES2294777T3 (de)
WO (1) WO1991017239A1 (de)

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DE69322266T2 (de) * 1992-04-03 1999-06-02 Perkin Elmer Corp Proben zusammensetzung und verfahren
DE4217134A1 (de) * 1992-05-23 1993-11-25 Boehringer Mannheim Gmbh Thermostabile Ligase aus Archaebakterien
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
DE69329824T2 (de) * 1992-08-03 2001-08-09 Abbott Lab Nachweis und amplifikation bestimmter nukleinsäuren mittels exonukleolytischer aktivität
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
DE69506393T2 (de) * 1994-04-04 1999-07-01 Ciba Corning Diagnostics Corp Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen
WO1996012824A2 (en) * 1994-10-21 1996-05-02 Abbott Laboratories Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample
AU3943095A (en) * 1994-11-04 1996-05-31 Genis Hf. A gene from the bacterium rhodothermus marinus, coding for the amino acid sequence of dna-ligase, produced in escherichia coli or some other suitable host organism
EP0920440B1 (de) * 1996-02-09 2012-08-22 Cornell Research Foundation, Inc. Detektion von unterschieden in der sequenz von nukleinsäuren mittels der ligasedetektionsreaktion und adressierbaren anordnungen
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (de) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Erkennung von Nukleinsäuresequenz-Unterschieden mittels der gleichzeitigen Ligaseerkennungs- und Polymerasekettenreaktion
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
AU776810B2 (en) * 1996-07-19 2004-09-23 Cornell Research Foundation Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
CA2348776C (en) * 1998-10-30 2008-03-25 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
ATE237399T1 (de) 1999-09-29 2003-05-15 Tecan Trading Ag Thermocycler sowie hebeelement für mikrotiterplatte
US6272939B1 (en) 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
US7169355B1 (en) * 2000-02-02 2007-01-30 Applera Corporation Apparatus and method for ejecting sample well trays
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
US6719949B1 (en) 2000-06-29 2004-04-13 Applera Corporation Apparatus and method for transporting sample well trays
US6514750B2 (en) 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
US6942836B2 (en) 2001-10-16 2005-09-13 Applera Corporation System for filling substrate chambers with liquid
US7309575B2 (en) * 2002-01-16 2007-12-18 The University Of North Carolina At Chapel Hill Protein purification and detection methods
US20040258563A1 (en) 2003-06-23 2004-12-23 Applera Corporation Caps for sample wells and microcards for biological materials
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
WO2005108618A2 (en) 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
ATE489396T1 (de) 2004-08-24 2010-12-15 Cornell Res Foundation Inc Detektion von nucleinsäureunterschieden mit hilfe von endonucleasespaltungs-/ligaseabgabereaktionen und kapillarelektrophoresen oder mikroarrays
CA2619841C (en) 2005-08-16 2012-07-03 Merlogen, Llc Methods for identifying merle mutations in the silv gene
US7285388B1 (en) 2006-04-07 2007-10-23 Merlogen, Llc Methods for identification of alport syndrome
WO2009087686A2 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection methods
WO2009087687A1 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection process and uses thereof
CN113481281A (zh) 2014-10-08 2021-10-08 康奈尔大学 一种用于鉴定样品中的含有靶核苷酸序列的一个或多个核酸分子的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
AU3539089A (en) * 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles

Also Published As

Publication number Publication date
JPH05508764A (ja) 1993-12-09
JP2001269187A (ja) 2001-10-02
EP0528882A1 (de) 1993-03-03
EP1507000B1 (de) 2010-03-03
WO1991017239A1 (en) 1991-11-14
EP0528882B1 (de) 2007-10-10
DE69133582T2 (de) 2008-07-24
ATE459709T1 (de) 2010-03-15
EP0528882A4 (en) 1993-09-08
EP1507000A2 (de) 2005-02-16
DK0528882T3 (da) 2008-01-14
ES2294777T3 (es) 2008-04-01
EP1507000A3 (de) 2006-03-29
JP3889807B2 (ja) 2007-03-07
DE69133629D1 (de) 2010-04-15
ATE375392T1 (de) 2007-10-15
JP2004089204A (ja) 2004-03-25

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