ATE375392T1 - Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase - Google Patents

Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase

Info

Publication number
ATE375392T1
ATE375392T1 AT91909119T AT91909119T ATE375392T1 AT E375392 T1 ATE375392 T1 AT E375392T1 AT 91909119 T AT91909119 T AT 91909119T AT 91909119 T AT91909119 T AT 91909119T AT E375392 T1 ATE375392 T1 AT E375392T1
Authority
AT
Austria
Prior art keywords
nucleic acid
amplification system
dna amplification
genetic diseases
thermostable ligase
Prior art date
Application number
AT91909119T
Other languages
English (en)
Inventor
Francis Barany
John Zebala
Deborah Nickerson
Robert Kaiser
Leroy Hood
Original Assignee
Cornell Res Foundation Inc
California Inst Of Techn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc, California Inst Of Techn filed Critical Cornell Res Foundation Inc
Application granted granted Critical
Publication of ATE375392T1 publication Critical patent/ATE375392T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT91909119T 1990-05-03 1991-04-29 Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase ATE375392T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US51844790A 1990-05-03 1990-05-03

Publications (1)

Publication Number Publication Date
ATE375392T1 true ATE375392T1 (de) 2007-10-15

Family

ID=24063976

Family Applications (2)

Application Number Title Priority Date Filing Date
AT91909119T ATE375392T1 (de) 1990-05-03 1991-04-29 Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase
AT04077667T ATE459709T1 (de) 1990-05-03 1991-04-29 Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT04077667T ATE459709T1 (de) 1990-05-03 1991-04-29 Hitzestabiles ligase enthaltendes dns- amplifikationssystem zur bestimmung von genetischen krankheiten

Country Status (7)

Country Link
EP (2) EP0528882B1 (de)
JP (3) JP3889807B2 (de)
AT (2) ATE375392T1 (de)
DE (2) DE69133629D1 (de)
DK (1) DK0528882T3 (de)
ES (1) ES2294777T3 (de)
WO (1) WO1991017239A1 (de)

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EP0636186B1 (de) 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Proben zusammensetzung und verfahren
DE4217134A1 (de) * 1992-05-23 1993-11-25 Boehringer Mannheim Gmbh Thermostabile Ligase aus Archaebakterien
US5700672A (en) * 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
JP3516953B2 (ja) * 1992-08-03 2004-04-05 アボツト・ラボラトリーズ エキソヌクレオリチック活性を用いた標的核酸の検出及び増幅
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
WO1995027078A1 (en) * 1994-04-04 1995-10-12 Ciba Corning Diagnostics Corp. Hibridization-ligation assays for the detection of specific nucleic acid sequences
CA2202990A1 (en) * 1994-10-21 1996-05-02 Alan H. Davis Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample
WO1996014403A1 (en) * 1994-11-04 1996-05-17 Genis Hf. A gene from the bacterium rhodothermus marinus, coding for the amino acid sequence of dna-ligase, produced in escherichia coli or some other suitable host organism
EP2332958B1 (de) * 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detektion von Unterschieden in der Sequenz von Nukleinsäuren mittels der Ligasedetektionsreaktion und adressierbaren Arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AU730633B2 (en) 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
AU776810B2 (en) * 1996-07-19 2004-09-23 Cornell Research Foundation Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
ATE362986T1 (de) * 1998-10-30 2007-06-15 Cornell Res Foundation Inc High fidelity thermostabile ligase und ihre verwendung
US7014994B1 (en) 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
ATE237399T1 (de) 1999-09-29 2003-05-15 Tecan Trading Ag Thermocycler sowie hebeelement für mikrotiterplatte
US6272939B1 (en) 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
US7169355B1 (en) 2000-02-02 2007-01-30 Applera Corporation Apparatus and method for ejecting sample well trays
JP5508654B2 (ja) 2000-04-14 2014-06-04 コーネル・リサーチ・ファンデーション・インコーポレイテッド リガーゼ検出反応を用いた核酸配列の違いの検出のための位置特定可能なアレイの設計方法
US6719949B1 (en) 2000-06-29 2004-04-13 Applera Corporation Apparatus and method for transporting sample well trays
US6514750B2 (en) 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
US6942836B2 (en) 2001-10-16 2005-09-13 Applera Corporation System for filling substrate chambers with liquid
US7309575B2 (en) * 2002-01-16 2007-12-18 The University Of North Carolina At Chapel Hill Protein purification and detection methods
US20040258563A1 (en) 2003-06-23 2004-12-23 Applera Corporation Caps for sample wells and microcards for biological materials
US7427479B2 (en) 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7364855B2 (en) 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
WO2006023919A2 (en) 2004-08-24 2006-03-02 Cornell Research Foundation, Inc. Detection of nucleic acid differences using endonuclease cleavage/ligas releasing reactions and capillary electrophoresis or microarrays
CA2619841C (en) 2005-08-16 2012-07-03 Merlogen, Llc Methods for identifying merle mutations in the silv gene
US7285388B1 (en) 2006-04-07 2007-10-23 Merlogen, Llc Methods for identification of alport syndrome
WO2009087686A2 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection methods
WO2009087687A1 (en) * 2008-01-04 2009-07-16 Premas Biotech Pvt.Ltd Nucleic acid based detection process and uses thereof
CA2963687C (en) 2014-10-08 2023-03-28 Cornell University Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles

Also Published As

Publication number Publication date
ATE459709T1 (de) 2010-03-15
JP2001269187A (ja) 2001-10-02
DE69133629D1 (de) 2010-04-15
EP1507000A3 (de) 2006-03-29
WO1991017239A1 (en) 1991-11-14
JP3889807B2 (ja) 2007-03-07
EP1507000A2 (de) 2005-02-16
ES2294777T3 (es) 2008-04-01
DK0528882T3 (da) 2008-01-14
DE69133582T2 (de) 2008-07-24
DE69133582D1 (de) 2007-11-22
EP0528882A1 (de) 1993-03-03
EP0528882A4 (en) 1993-09-08
EP0528882B1 (de) 2007-10-10
JPH05508764A (ja) 1993-12-09
EP1507000B1 (de) 2010-03-03
JP2004089204A (ja) 2004-03-25

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