ATE362986T1 - High fidelity thermostabile ligase und ihre verwendung - Google Patents

High fidelity thermostabile ligase und ihre verwendung

Info

Publication number
ATE362986T1
ATE362986T1 AT99956776T AT99956776T ATE362986T1 AT E362986 T1 ATE362986 T1 AT E362986T1 AT 99956776 T AT99956776 T AT 99956776T AT 99956776 T AT99956776 T AT 99956776T AT E362986 T1 ATE362986 T1 AT E362986T1
Authority
AT
Austria
Prior art keywords
ligase
thermostable ligase
high fidelity
reaction process
fidelity thermostable
Prior art date
Application number
AT99956776T
Other languages
English (en)
Inventor
Francis Barany
Weiguo Cao
Jie Tong
Original Assignee
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Res Foundation Inc filed Critical Cornell Res Foundation Inc
Application granted granted Critical
Publication of ATE362986T1 publication Critical patent/ATE362986T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Vessels, Lead-In Wires, Accessory Apparatuses For Cathode-Ray Tubes (AREA)
  • Confectionery (AREA)
  • Dowels (AREA)
AT99956776T 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung ATE362986T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10646198P 1998-10-30 1998-10-30

Publications (1)

Publication Number Publication Date
ATE362986T1 true ATE362986T1 (de) 2007-06-15

Family

ID=22311532

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99956776T ATE362986T1 (de) 1998-10-30 1999-10-29 High fidelity thermostabile ligase und ihre verwendung

Country Status (7)

Country Link
EP (1) EP1127135B1 (de)
JP (1) JP4598275B2 (de)
AT (1) ATE362986T1 (de)
AU (1) AU776820B2 (de)
CA (1) CA2348776C (de)
DE (1) DE69936147T2 (de)
WO (1) WO2000026381A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949370B1 (en) 1998-10-30 2005-09-27 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
WO2001080123A1 (en) * 2000-04-14 2001-10-25 Livecapital, Inc. Online credit services brokering
WO2005030995A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
KR20190116282A (ko) 2017-02-10 2019-10-14 지머젠 인코포레이티드 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략
JP2021533773A (ja) 2018-08-15 2021-12-09 ザイマージェン インコーポレイテッド ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用
US20230340449A1 (en) 2020-07-17 2023-10-26 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN117247911B (zh) * 2023-08-22 2024-06-07 武汉爱博泰克生物科技有限公司 大肠杆菌dna连接酶突变体及其在毕赤酵母中表达纯化方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0528882B1 (de) * 1990-05-03 2007-10-10 Cornell Research Foundation, Inc. Ein system der dna amplifikation zur detektion genetischer erkrankungen durch eine thermostabile ligase
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction

Also Published As

Publication number Publication date
WO2000026381A3 (en) 2000-11-09
JP4598275B2 (ja) 2010-12-15
DE69936147D1 (de) 2007-07-05
WO2000026381A2 (en) 2000-05-11
CA2348776C (en) 2008-03-25
DE69936147T2 (de) 2008-01-24
EP1127135B1 (de) 2007-05-23
CA2348776A1 (en) 2000-05-11
JP2002528121A (ja) 2002-09-03
AU1331000A (en) 2000-05-22
EP1127135A2 (de) 2001-08-29
AU776820B2 (en) 2004-09-23

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