DE60133992D1 - Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen - Google Patents

Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen

Info

Publication number
DE60133992D1
DE60133992D1 DE60133992T DE60133992T DE60133992D1 DE 60133992 D1 DE60133992 D1 DE 60133992D1 DE 60133992 T DE60133992 T DE 60133992T DE 60133992 T DE60133992 T DE 60133992T DE 60133992 D1 DE60133992 D1 DE 60133992D1
Authority
DE
Germany
Prior art keywords
nucleic acid
chimeric nucleic
polymerases
acid polymerases
thermostabile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60133992T
Other languages
English (en)
Inventor
Dirk Loeffert
Andreas Missel
Jie Kang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Application granted granted Critical
Publication of DE60133992D1 publication Critical patent/DE60133992D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
DE60133992T 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen Expired - Lifetime DE60133992D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50615300A 2000-02-17 2000-02-17
PCT/EP2001/001790 WO2001061015A2 (de) 2000-02-17 2001-02-16 Thermostable chimeric nucleic acid polymerases and uses thereof

Publications (1)

Publication Number Publication Date
DE60133992D1 true DE60133992D1 (de) 2008-06-26

Family

ID=24013413

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60133992T Expired - Lifetime DE60133992D1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
DE60143632T Expired - Lifetime DE60143632D1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre Nukleinsäurepolymerasen und ihre Verwendungen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE60143632T Expired - Lifetime DE60143632D1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre Nukleinsäurepolymerasen und ihre Verwendungen

Country Status (5)

Country Link
US (2) US8637288B2 (de)
EP (2) EP1925669B1 (de)
AT (2) ATE395420T1 (de)
DE (2) DE60133992D1 (de)
WO (1) WO2001061015A2 (de)

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US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
WO2004087868A2 (en) 2003-03-25 2004-10-14 Stratagene Dna polymerase fusions and uses thereof
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US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
US20090325169A1 (en) 2008-04-30 2009-12-31 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
ES2716402T3 (es) * 2008-11-03 2019-06-12 Kapa Biosystems Inc ADN polimerasas quiméricas
WO2010132508A2 (en) * 2009-05-11 2010-11-18 Biomatrica, Inc. Compositions and methods for biological sample storage
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
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CN106572650B (zh) 2014-06-10 2021-08-31 生物马特里卡公司 在环境温度下稳定凝血细胞
EP3387002A4 (de) 2015-12-07 2019-04-24 Bio-Rad Laboratories, Inc. Dimere umkehrtranskriptase
AU2016368265B2 (en) 2015-12-08 2021-10-28 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
WO2021127848A1 (zh) * 2019-12-23 2021-07-01 深圳华大生命科学研究院 嵌合dna聚合酶及其应用
CN117050967B (zh) * 2023-08-21 2024-04-09 南京诺唯赞生物科技股份有限公司 一种改善二代测序文库gc均衡性的方法

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Also Published As

Publication number Publication date
US20030138805A1 (en) 2003-07-24
EP1925669B1 (de) 2010-12-08
ATE491031T1 (de) 2010-12-15
EP1259624A2 (de) 2002-11-27
ATE395420T1 (de) 2008-05-15
DE60143632D1 (de) 2011-01-20
EP1259624B1 (de) 2008-05-14
US20140154748A1 (en) 2014-06-05
WO2001061015A2 (de) 2001-08-23
US8637288B2 (en) 2014-01-28
EP1925669A1 (de) 2008-05-28
WO2001061015A3 (de) 2002-04-25

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