ATE348881T1 - Thermostabiles taq polymerase fragment - Google Patents

Thermostabiles taq polymerase fragment

Info

Publication number
ATE348881T1
ATE348881T1 AT04018913T AT04018913T ATE348881T1 AT E348881 T1 ATE348881 T1 AT E348881T1 AT 04018913 T AT04018913 T AT 04018913T AT 04018913 T AT04018913 T AT 04018913T AT E348881 T1 ATE348881 T1 AT E348881T1
Authority
AT
Austria
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
AT04018913T
Other languages
English (en)
Inventor
Waltraud Dr Ankenbauer
Thomas Dr Meier
Annette Dr Deufel
Dieter Dr Heindl
Gisela Dr Betzl
Rainer Dr Schmuck
Bernd Dr Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE348881T1 publication Critical patent/ATE348881T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT04018913T 2003-08-12 2004-08-10 Thermostabiles taq polymerase fragment ATE348881T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
ATE348881T1 true ATE348881T1 (de) 2007-01-15

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04018913T ATE348881T1 (de) 2003-08-12 2004-08-10 Thermostabiles taq polymerase fragment

Country Status (15)

Country Link
US (1) US7972830B2 (de)
EP (1) EP1507002B1 (de)
JP (1) JP3929061B2 (de)
KR (1) KR100626578B1 (de)
CN (1) CN1590543B (de)
AT (1) ATE348881T1 (de)
AU (1) AU2004203649B2 (de)
BR (1) BRPI0403221A (de)
CA (1) CA2474734C (de)
DE (1) DE602004003755T2 (de)
DK (1) DK1507002T3 (de)
ES (1) ES2278249T3 (de)
HK (1) HK1074458A1 (de)
MX (1) MXPA04007721A (de)
SG (1) SG109552A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006074217A2 (en) * 2005-01-04 2006-07-13 Stratagene California Hot start polymerase reaction using a thermolabile blocker
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (de) 2008-04-28 2009-11-04 Biotype AG Stoffe und Verfahren für Profilierungstest auf DNA-Basis
JP5539325B2 (ja) 2008-04-30 2014-07-02 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 修飾されたｒｎａ単量体を用いるｒｎアーゼｈを基礎とするアッセイ
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
EP2501716B1 (de) * 2009-11-19 2015-01-21 Solis Biodyne Zusammensetzungen zur erhöhung der Polypeptidstabilität und -aktivität sowie zugehörige Verfahren
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
WO2012170908A1 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
WO2015061714A1 (en) 2013-10-25 2015-04-30 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP3795681B1 (de) * 2014-12-16 2024-09-18 Life Technologies Corporation Polymerasezusammensetzungen und verfahren zur herstellung und verwendung davon
CN107636164B (zh) 2015-05-29 2022-02-18 豪夫迈·罗氏有限公司 用柠康酸酐灭活微生物的方法
WO2017090684A1 (ja) * 2015-11-27 2017-06-01 国立大学法人九州大学 Ｄｎａポリメラーゼ変異体
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
US20200181666A1 (en) * 2017-03-29 2020-06-11 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
EP3740591A4 (de) * 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutierte dna-polymerasen
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
BR112022021086A2 (pt) 2020-04-30 2022-12-13 Stab Vida Investig E Servicos Em Ciencias Biologicas Lda Sistema integrado para a detecção e identificação de sequências específicas de ácidos nucleicos e método de utilização do sistema integrado
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
FR2652520B1 (fr) 1989-10-02 1992-02-07 Sgn Soc Gen Tech Nouvelle Procede et dispositif pour maintenir une atmosphere propre a temperature regulee sur un poste de travail.
WO1991005753A1 (en) 1989-10-16 1991-05-02 E.I. Du Pont De Nemours And Company Process for chlorofluoropropanes
ES2134198T3 (es) * 1990-09-28 1999-10-01 Hoffmann La Roche Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.
WO1992006188A2 (en) 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5885813A (en) 1995-05-31 1999-03-23 Amersham Life Science, Inc. Thermostable DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
DK1069131T3 (da) 1999-07-15 2006-06-26 Qiagen Gmbh Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler
GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
JP4768155B2 (ja) 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro

Also Published As

Publication number Publication date
ES2278249T3 (es) 2007-08-01
DK1507002T3 (da) 2007-04-10
US7972830B2 (en) 2011-07-05
AU2004203649B2 (en) 2006-01-12
SG109552A1 (en) 2005-03-30
CN1590543B (zh) 2010-05-12
KR100626578B1 (ko) 2006-09-25
AU2004203649A1 (en) 2005-03-03
DE602004003755D1 (de) 2007-02-01
CA2474734A1 (en) 2005-02-12
CN1590543A (zh) 2005-03-09
JP3929061B2 (ja) 2007-06-13
EP1507002B1 (de) 2006-12-20
BRPI0403221A (pt) 2005-07-12
US20050037412A1 (en) 2005-02-17
DE602004003755T2 (de) 2007-10-11
HK1074458A1 (en) 2005-11-11
EP1507002A3 (de) 2005-07-27
EP1507002A2 (de) 2005-02-16
MXPA04007721A (es) 2005-07-12
CA2474734C (en) 2010-07-13
JP2005058236A (ja) 2005-03-10
KR20050025266A (ko) 2005-03-14

Similar Documents

Publication Publication Date Title
HK1074458A1 (en) Thermostable taq polymerase fragment
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DE602004025801D1 (de) Dna-polymerasefusionen und deren verwendungen
DE69426400D1 (de) Thermostable Nukleinsäure Polymerase
ATE364718T1 (de) Verfahren zur vervielfältigung von nukleinsäure
DK0550687T3 (da) 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser
ATE325877T1 (de) Mutierte chimäre dns-polymerasen
ATE302279T1 (de) Kälteempfindliche dna-polymerasemutanten
DE60110564D1 (en) Rna polymerase mutanten mit erhöhter thermostabilität
HUP0303517A2 (hu) Hímfertilitást közvetítő nukleotidszekvenciák és alkalmazásuk
DE60301325D1 (de) Mutation von dna polymerasen aus archaeobakterien
DE60332781D1 (de) Verfahren und Zusammensetzungen zur Identifizierung und Charakterisierung von Hepatitis C
DE602005023586D1 (de) Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'-nichttranslatierten bereichs von hcv
DE50115782D1 (de) THERMOSTABILE POLYMERASE AUS i THERMOCOCCUS PACIFICUS /i
Ikeuchi et al. The enhancing of a cysteine proteinase activity at acidic pH by protein engineering, the role of glutamic 50 in the enzyme mechanism of caricain
DK2186898T3 (da) Blomstrings-induktion
IL195194A0 (en) Thermostable dna polymerase of the archaeal ampullavirus abv and its applications
DK1789541T3 (da) Overeksprimeret og renset Aspergillus ficuum-oxidase og nukleinsyre, der koder derfor
ATE426817T1 (de) Verfahren zur identifizierung von proteinfaltungsinhibitoren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties