DE60301325D1 - Mutation von dna polymerasen aus archaeobakterien - Google Patents

Mutation von dna polymerasen aus archaeobakterien

Info

Publication number
DE60301325D1
DE60301325D1 DE60301325T DE60301325T DE60301325D1 DE 60301325 D1 DE60301325 D1 DE 60301325D1 DE 60301325 T DE60301325 T DE 60301325T DE 60301325 T DE60301325 T DE 60301325T DE 60301325 D1 DE60301325 D1 DE 60301325D1
Authority
DE
Germany
Prior art keywords
polymerase
wild
variant
type
archaeobacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60301325T
Other languages
English (en)
Other versions
DE60301325T2 (de
Inventor
Bernard Connolly
Mark Fogg
Laurence Pearl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Newcastle, The
Newcastle University of Upon Tyne
Original Assignee
University of Newcastle, The
Newcastle University of Upon Tyne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Newcastle, The, Newcastle University of Upon Tyne filed Critical University of Newcastle, The
Publication of DE60301325D1 publication Critical patent/DE60301325D1/de
Application granted granted Critical
Publication of DE60301325T2 publication Critical patent/DE60301325T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60301325T 2002-04-17 2003-04-15 Mutation von dna polymerasen aus archaeobakterien Expired - Lifetime DE60301325T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0208768.2A GB0208768D0 (en) 2002-04-17 2002-04-17 DNA polymerases
GB0208768 2002-04-17
PCT/GB2003/001623 WO2003089637A1 (en) 2002-04-17 2003-04-15 Mutation of dna polymerases from archaeobacteria

Publications (2)

Publication Number Publication Date
DE60301325D1 true DE60301325D1 (de) 2005-09-22
DE60301325T2 DE60301325T2 (de) 2006-05-24

Family

ID=9934981

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60301325T Expired - Lifetime DE60301325T2 (de) 2002-04-17 2003-04-15 Mutation von dna polymerasen aus archaeobakterien

Country Status (10)

Country Link
US (1) US8557554B2 (de)
EP (1) EP1463809B1 (de)
JP (1) JP4395377B2 (de)
AT (1) ATE302270T1 (de)
AU (1) AU2003226543B2 (de)
CA (1) CA2475418C (de)
DE (1) DE60301325T2 (de)
DK (1) DK1463809T3 (de)
GB (1) GB0208768D0 (de)
WO (1) WO2003089637A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507012A (ja) * 2002-10-25 2006-03-02 ストラタジーン カリフォルニア 減少した塩基類似体検出活性を有するｄｎａポリメラーゼ
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
CN101368214B (zh) * 2008-09-24 2010-09-15 上海大学 用于古细菌多样性分析的针对16S rRNA基因的引物
JP5258512B2 (ja) * 2008-10-30 2013-08-07 株式会社日立製作所 エキソヌクレアーゼ活性増強ｄｎａポリメラーゼ変異体
CN103857805B (zh) * 2011-08-10 2017-07-18 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
JP2014000015A (ja) * 2012-06-15 2014-01-09 Kaneka Corp 核酸解析法
JP6478444B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
JP6493209B2 (ja) * 2013-08-06 2019-04-03 東洋紡株式会社 核酸増幅法
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
WO2015085230A1 (en) 2013-12-06 2015-06-11 Bio-Rad Laboratories, Inc. Fusion polymerases
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
WO2017058810A2 (en) 2015-10-01 2017-04-06 Life Technologies Corporation Polymerase compositions and kits, and methods of using and making the same
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
CN112899353A (zh) * 2021-01-05 2021-06-04 南京普济生物有限公司 一种用于dna聚合酶识别特异性的缓冲液及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
EP1088891B1 (de) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation
EP1232270A1 (de) 1999-11-23 2002-08-21 Amersham Biosciences Corp. Verbesserter dideoxynucleotidtriphosphatverbrauch durch hyperthermophile dna polymerase aus dem archaebakterium i(pyrococcus furiosus)

Also Published As

Publication number Publication date
DK1463809T3 (da) 2005-12-19
EP1463809A1 (de) 2004-10-06
AU2003226543A1 (en) 2003-11-03
CA2475418C (en) 2013-06-11
AU2003226543B2 (en) 2004-11-04
US8557554B2 (en) 2013-10-15
US20060057682A1 (en) 2006-03-16
JP4395377B2 (ja) 2010-01-06
ATE302270T1 (de) 2005-09-15
GB0208768D0 (en) 2002-05-29
JP2005526510A (ja) 2005-09-08
DE60301325T2 (de) 2006-05-24
EP1463809B1 (de) 2005-08-17
CA2475418A1 (en) 2003-10-30
WO2003089637A1 (en) 2003-10-30

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